The aim of the research was to create genetic variations in Jasminum sambac using oryzalin as a polyploidizing agent. Rooted stem cuttings of J. sambac were treated with oryzalin (0.005, 0.01 and 0.05%) and their vegetative and flowering parameters were recorded on 150 days after treatment. The study revealed that the higher concentration of oryzalin (0.05%) caused reduction in plant height, internodal length, number of flowering cymes per plant and number of flower buds per cyme. However, the same treatment significantly enhanced certain vegetative parameters viz., number of secondary branches, stem girth, number of leaves and leaf area, and flowering parameters namely flower bud length, corolla tube length, flower bud girth, diameter of open flower and hundred flower bud weight. High GCV was recorded in major yield attributing traits, i.e. number of flowering cymes per plant and hundred flower bud weight. High heritability and genetic advance registered for the traits viz., plant height, number of secondary branches, internodal length, number of flowering cymes, flower bud girth and hundred flower bud weight per plant indicates least influence by environment and selection such characters may be useful.