Orthogonal Settings Research Articles

Evaluation of Formulated Jatropha Oil as Cooling and Lubricating Cutting Fluids in Turning of AISI 1525 Steel Employing Taguchi-TOPSIS Technique

This study examined the impact of cutting parameters and fluids on machining performance metrics, such as machine vibration rate and sound level, when turning AISI 1525 steel using tungsten carbide tools. Jatropha oil was used in two forms, minimum quantity lubrication and emulsion. Jatropha MQL was applied directly to the cutting region without any additives or water. Jatropha emulsion was formulated based on 44 full factorial techniques. Jatropha emulsion was formulated by mixing water, biocide, anticorrosive agent, antifoam agent, and emulsifier. The pH of the emulsified sample was used to determine the best formulation through optimization. Jatropha emulsion and Jatropha MQL were compared with their mineral oil equivalent during machining under Taguchi L9 orthogonal array settings. The hardness of the workpiece was determined at every 5 mm diameter. Additionally, the microstructure of the workpiece was examined at 5 mm, 35 mm, and 70 mm diameters of the shaft. Multi-response optimization was performed using TOPSIS to determine optimal cutting parameters to minimize machine vibration rate and machine sound level. Results showed that jatropha MQL and jatropha emulsion reduced machine vibration rate drastically as compared to mineral oil counterparts. Jatropha MQL surpassed jatropha emulsion, mineral emulsion, and mineral MQL by 75.8%, 81.2%, and 90.5%, respectively. In terms of sound intensity, Jatropha oil MQL performed significantly better than other cooling and lubricating fluids based on general process parameter results. The hardness of the material increases as the diameter increases and it varies between 70.2 HBR and 150.4 HBR. Microstructural analysis showed the presence of pearlites and ferrites on the selected shaft diameters. Findings showed that the lowest machine vibration and machine sound values were achieved with experimental trial 1 such as spindle speed (355 rev/min), feed rate (0.10 mm/rev), and depth of cut (0.75 mm) in all cases of machining fluid. The optimal solutions of spindle speed, feed rate, and depth of cut were 355 rev/min, 0.10 mm/rev, and 0.75 mm; 355 rev/min, 0.15 mm/rev, and 1.00 mm for machine sound and machine vibrations, respectively.

The structure, centrality, and scale of urban street networks: Cases from Pre-Industrial Afro-Eurasia.

Cities and towns have often developed infrastructure that enabled a variety of socio-economic interactions. Street networks within these urban settings provide key access to resources, neighborhoods, and cultural facilities. Studies on settlement scaling have also demonstrated that a variety of urban infrastructure and resources indicate clear population scaling relationships in both modern and ancient settings. This article presents an approach that investigates past street network centrality and its relationship to population scaling in urban contexts. Centrality results are compared statistically among different urban settings, which are categorized as orthogonal (i.e., planned) or self-organizing (i.e., organic) urban settings, with places having both characteristics classified as hybrid. Results demonstrate that street nodes have a power law relationship to urban area, where the number of nodes increases and node density decreases in a sub-linear manner for larger sites. Most median centrality values decrease in a negative sub-linear manner as sites are larger, with organic and hybrid urban sites' centrality being generally less and diminishing more rapidly than orthogonal settings. Diminishing centrality shows comparability to modern urban systems, where larger urban districts may restrict overall interaction due to increasing transport costs over wider areas. Centrality results indicate that scaling results have multiples of approximately ⅙ or ⅓ that are comparable to other urban and road infrastructure, suggesting a potential relationship between different infrastructure features and population in urban centers. The results have implications for archaeological settlements where urban street plans are incomplete or undetermined, as it allows forecasts to be made on past urban sites' street network centrality. Additionally, a tool to enable analysis of street networks and centrality is provided as part of the contribution.

Development and Profiling of Inverse Agonist Tools for the Neuroprotective Transcription Factor Nurr1.

The ligand-sensing transcription factor nuclear receptor related 1 (Nurr1) evolves as an appealing target to treat neurodegenerative diseases. Despite its therapeutic potential observed in various rodent models, potent modulators for Nurr1 are lacking as pharmacological tools. Here, we report the structure-activity relationship and systematic optimization of indole-based inverse Nurr1 agonists. Optimized analogues decreased the receptor's intrinsic transcriptional activity by up to more than 90% and revealed preference for inhibiting Nurr1 monomer activity. In orthogonal cell-free settings, we detected displacement of NCoRs and disruption of the Nurr1 homodimer as molecular modes of action. The inverse Nurr1 agonists reduced the expression of Nurr1-regulated genes in T98G cells, and treatment with an inverse Nurr1 agonist mimicked the effect of Nurr1 silencing on interleukin-6 release from LPS-stimulated human astrocytes. The indole-based inverse Nurr1 agonists valuably extend the toolbox of Nurr1 modulators to further probe the role of Nurr1 in neuroinflammation, cancer, and beyond.

Abstract PD15-04: Trop-2 inactivation of E-cadherin drives triple negative breast cancer relapse

Abstract Hundreds of proteins/genes have been linked to the metastatic phenotype. However, consistent markers of tumour aggressiveness and metastatic potential in breast cancer patients were not identified, not even including proteomic analysis and large-scale genome sequencing.Metastasis-associated genes were predicted to include not only drivers of the metastatic phenotype, but also secondary events, together with adaptive, counterbalancing changes. Thus, to identify candidates with a required role in metastatic diffusion, we looked for genes that were concordantly dysregulated across orthogonal cancer metastasis settings. This led us to identify Trop-2 as uniquely upregulated and associated to metastasis in experimental models of breast cancer, as well as in other solid tumors. We identified functional inactivation of E-cadherin by Trop-2 as the main motor of metastatic diffusion of such metastatic systems. Trop-2 binding to E-cadherin inactivated its cell-cell adhesion function, through release from the cytoskeleton, for activation of β-catenin transcriptional activity. This led to anti-apoptotic signaling, increased cell migration capacity and enhanced cancer cell survival. We showed that this mechanism led to metastatic diffusion of xenotransplants growing in immunosuppressed mice. An E-cadherin-inactivation metastasis program was then shown to be recapitulated in breast cancer patients, as well as in other solid tumors, over 24 independent case series, encompassing 13,042 primary tumours. Aggressive triple-negative breast cancers were shown to be driven toward global relapse by Trop-2 overexpression, through E-cadherin inactivation and activation of β-catenin transcriptional activity. No disease recurrence was observed in control cases over +12 years of follow-up. These finding lead to a novel paradigm of a Trop-2-driven, E-cadherin-inactivation program as a main metastasis driver in solid tumors. This may open far-reaching perspectives in diagnostic procedures and anti-cancer therapies. Citation Format: Saverio Alberti, Marco Trerotola, Valeria Relli, Rossano Lattanzio, Martina Ceci, Khouloud Boujnah, Valeria Garbo, Antonino Moschella, Patrizia Querzoli, Massimo Pedriali, Laura Antolini, Emanuela Guerra. Trop-2 inactivation of E-cadherin drives triple negative breast cancer relapse [abstract]. In: Proceedings of the 2020 San Antonio Breast Cancer Virtual Symposium; 2020 Dec 8-11; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2021;81(4 Suppl):Abstract nr PD15-04.

Rift segment interaction in orthogonal and rotational extension experiments: Implications for the large-scale development of rift systems

During extension of the continental lithosphere, rift basins develop. These are often initially offset, and must interact and connect in order to create a continuous rift system that may ultimately achieve break-up. When simulating extensional tectonics and rift interaction structures, analogue and numerical modellers often apply a continuous extension rate along the strike of a rift or rift system. Yet in nature significant extension velocity variations occur along rifts and plate boundaries as a natural consequence of tectonic plates moving apart about a pole of rotation, resulting in rotational extension, and associated rift propagation and structural gradients. Here we present various analogue tectonic experiments to assess rift interaction structures forming in orthogonal extension settings versus rotational extension settings. Our modelling efforts show that rotational extension and orthogonal extension produce significantly different large-scale structures. Rotational extension can cause important variations in rift maturity between rift segments, delay rift interaction zone development, and make rift segments propagate in opposite directions. Still, local features in a rotational extension system can often be regarded as evolving in an orthogonal extension setting. Furthermore, we find that various degrees of rift underlap produce three basic modes of rift linkage structures. Low underlap distance (high angle φ) experiments develop rift pass structures. With increasing underlap distance (φ = ca. 40°), transfer zone basins develop. High degrees of underlap (φ ≤ 30°) tend to result in en echelon sub-basins. Our results match with data from previous modelling efforts and natural examples. We furthermore propose a large-scale tectonic scenario for the East African Rift System based on rotational extension and associated rift propagation. These insights may also be applicable when studying other large-scale rift systems.

A Method of High-Resolution Radiotherapy Delivery Fluences with a Pair of Fields with Orthogonal Collimator Settings: A Study on Ten Head-and-Neck Cancer Patients.

Context:Introduction of dual-layer multileaf collimator (MLC) radiotherapy linear accelerators into clinical practice is an important development in advanced external beam radiotherapy. A method of delivering comparable high-resolution fluences with a single-layer MLC is presented.Aims:The aims of this study are to present new algorithms and approaches to define high-resolution hypermodulated fluences, obtain orthogonal decomposition of fluences, and deliver them on a linear accelerator with single MLC from two perpendicular collimator settings.Materials and Methods:High-resolution fluences were defined using Monte Carlo (MC) calculation. A novel use of a limited-memory, bounded, Broyden–Fletcher–Goldfarb–Shanno algorithm was used to decompose such fluences to ones deliverable with a pair of fields with mutually orthogonal collimator settings. Such a technique, here named cross motion leaf calculator (XMLC), is compared against single sliding window (SSW) technique typically used in intensity-modulated radiation therapy (IMRT). An electronic portal imaging device (EPID) is used, and the results were compared with gamma analysis. Furthermore, MC was used to determine dose distributions for computed tomography images of ten head-and-neck cancer patients.Results:Gamma analysis (3%, 3 mm) against ideal fluence is considerably more favorable to XMLC (94% ± 4%) versus SSW (76% ± 5%). Furthermore, the dose–volume histogram (DVH) analysis showed that XMLC enables delivery of fluences superior to that of IMRT and these results in clinically relevant enhancements in DVH results.Conclusions:At the time of writing of this study, there were more than 12,000 medical linear accelerators in clinical use, and XMLC can prove itself useful wherever linac is equipped with MLC but cannot delivery latest techniques, such as volumetric modulated arc therapy.

Effects of sedimentation on rift segment evolution and rift interaction in orthogonal and oblique extensional settings: Insights from analogue models analysed with 4D X-ray computed tomography and digital volume correlation techniques

Abstract During the early evolution of rift systems, individual rift segments often develop along pre-existing crustal weaknesses that are frequently non-continuous and laterally offset. As extension progresses, these initial rift segments establish linkage in order to develop a continuous rift system that might eventually lead to continental break-up. Previous analogue and numerical modelling efforts have demonstrated that rift interaction structures are influenced by structural inheritances, detachment layers, magma bodies, rate and direction of extension, as well as the distance between rift segments. Yet to date, the effects of syn-tectonic sediments have been largely ignored or only modelled in 2D. In this study we therefore assess the influence of sedimentation on rift segment and rift interaction zone evolution in orthogonal and oblique extensional settings, by means of 3D brittle-ductile analogue models, analysed with 4D X-ray computed tomography (XRCT or CT) methods and digital volume correlation (DVC) techniques. Our models show that syn-rift sedimentation does not significantly influence the initial large-scale evolution of rift segments and rift interaction zones. Nevertheless, syn-rift sedimentation can strongly affect rift-internal structures: sedimentary loading reinforces the rift wedge, decreasing rift wedge faulting and increases subsidence within the rift basin. These effects are strongest in areas where most accommodation space is available, that is, along the main rift segments. In contrast, rift segments that undergo high degrees of oblique extension develop less accommodation space and are therefore less influenced by sedimentary loading. Rift interaction structures are least affected by sediment influx, as they experience relatively low amounts of subsidence so that little accommodation space is available. Our conclusions are valid for the early stages of rift development, when a high sediment influx could delay continental break-up, as other processes are likely to become dominant during later stages of continental extension. Finally, state-of-the-art DVC analysis of CT data proves to be a powerful tool to extract and fully quantify 3D internal model deformation in great detail and could be useful for comparing and calibrating analogue and numerical models.

Insights into the effects of oblique extension on continental rift interaction from 3D analogue and numerical models

Continental rifts often develop from linkage of distinct rift segments under varying degrees of extension obliquity. These rift segments arise from rift initiation at non-aligned crustal heterogeneities and need to interact to develop a full-scale rift system. Here, we test the effects of 1) oblique extension and 2) initial heterogeneity (seed) offset on continental rift interaction with the use of an improved analogue model set-up. X-ray computer tomography (CT) techniques are used to analyse the 3D models through time and the results are compared with additional numerical models and natural examples. The experimental results reveal that increasing extension obliquity strongly changes rift segment structures from wide rifts in orthogonal settings to narrower rifts with oblique internal structures under oblique extension conditions to narrow strike-slip dominated systems towards the strike-slip domain. We also find that both decreasing seed offset and increasing extension obliquity promote hard linkage of rift segments through the formation of continuous rift boundary faults at the surface. (Initial) soft linkage through the formation of relay ramps is more likely when seed offset increases or extension is more orthogonal. Rather than linking at depth, the rift boundary faults curve around each other at depth and merge towards the surface to form a continuous trough. Orthogonal extension promotes the formation of intra-rift horsts, which may provide hydrocarbon traps in nature.

ChemInform Abstract: Orthogonality in Discrete Self‐Assembly — Survey of Current Concepts

AbstractReview: <180 refs.

Orthogonality in discrete self-assembly – survey of current concepts

Over years, mathematicians, biologists and chemists have capitalised on the highly useful concept of orthogonality for developing sophisticated complex systems. The use of orthogonal pairs ensures that any modification made on one pair does not propagate any effect onto the other. While the concept equally pertains to dynamic supramolecular interactions, interference-free self-assembly built on multiple orthogonal interactions is still limited and the underlying notions are not yet firmly established. Herein, we identify, classify and evaluate dynamic interactions in various orthogonal settings in order to distill out general recommendations for reliable dynamic orthogonality. Our classification has to exclude templating, allosteric and/or cooperative effects as the latter are specific for individual cases only.

Phonological Mismatch Makes Aided Speech Recognition in Noise Cognitively Taxing: Retracted Article

The working memory framework for Ease of Language Understanding predicts that speech processing becomes more effortful, thus requiring more explicit cognitive resources, when there is mismatch between speech input and phonological representations in long-term memory. To test this prediction, we changed the compression release settings in the hearing instruments of experienced users and allowed them to train for 9 weeks with the new settings. After training, aided speech recognition in noise was tested with both the trained settings and orthogonal settings. We postulated that training would lead to acclimatization to the trained setting, which in turn would involve establishment of new phonological representations in long-term memory. Further, we postulated that after training, testing with orthogonal settings would give rise to phonological mismatch, associated with more explicit cognitive processing. Thirty-two participants (mean=70.3 years, SD=7.7) with bilateral sensorineural hearing loss (pure-tone average=46.0 dB HL, SD=6.5), bilaterally fitted for more than 1 year with digital, two-channel, nonlinear signal processing hearing instruments and chosen from the patient population at the Linköping University Hospital were randomly assigned to 9 weeks training with new, fast (40 ms) or slow (640 ms), compression release settings in both channels. Aided speech recognition in noise performance was tested according to a design with three within-group factors: test occasion (T1, T2), test setting (fast, slow), and type of noise (unmodulated, modulated) and one between-group factor: experience setting (fast, slow) for two types of speech materials-the highly constrained Hagerman sentences and the less-predictable Hearing in Noise Test (HINT). Complex cognitive capacity was measured using the reading span and letter monitoring tests. PREDICTION: We predicted that speech recognition in noise at T2 with mismatched experience and test settings would be associated with more explicit cognitive processing and thus stronger correlations with complex cognitive measures, as well as poorer performance if complex cognitive capacity was exceeded. Under mismatch conditions, stronger correlations were found between performance on speech recognition with the Hagerman sentences and reading span, along with poorer speech recognition for participants with low reading span scores. No consistent mismatch effect was found with HINT. The mismatch prediction generated by the working memory framework for Ease of Language Understanding is supported for speech recognition in noise with the highly constrained Hagerman sentences but not the less-predictable HINT.

Signal-averaged electrocardiography in normal newborn infants.

Signal-averaged electrocardiograms (SAECGs) were recorded with bipolar orthogonal and standard 12-lead settings in 29 term healthy newborn infants. SAECGs performed with the two lead configurations were similar in quality and quantity. For the duration of the QRS complex (QRSD), the upper limit of normality for newborn infants can be set to 100 ms. It is similar in individual bipolar orthogonal and individual standard 12-lead recordings as well as in filtered QRSD in the vectorcardiograms (the vector magnitude, VM, and the sum of extremity leads). In individual leads of bipolar orthogonal and standard 12-lead recordings, root-mean-square voltage of the terminal 40 ms of the QRS complex (RMS40) is widely scattered and unsuitable as discriminant variable. For VM and the sum of the extremity leads, the same reference values for RMS40 and duration of low-amplitude signal can be used as applied in adults. Individual lead recordings provide complementary information in addition to vectorcardiograms. There are practical advantages using standard 12-lead compared to conventional bipolar orthogonal configurations. The widespread use of standard 12-lead ECG in routine medical practice makes its utility advantageous also in performing signal-averaged electrocardiography.

Virtual micro-intensity modulated radiation therapy.

Virtual micro-intensity modulated radiation therapy (VMIMRT) combines a 10 x 5 mm2 intensity map with a 5 x 10 mm2 intensity map, delivered at orthogonal collimator settings. The superposition of these component maps (CM) yields a 5 x 5 mm2 virtual micro-intensity map (VMIM) that can be delivered with a 1 cm leaf width MLC. A pair of CMs with optimal delivery efficiency and quality must be chosen, since a given VMIM can be delivered using several different pairs. This is possible since, for each group of four VMIM cells that can be covered by an MLC leaf in either collimator orientation, the minimum intensity can be delivered from either collimator setting. By varying the proportions of the minimum values that go into each CM, one can simultaneously minimize the number of potential junction effects and the number of segments required to deliver the VMIM. The minimization is achieved by reducing high leaf direction gradients in the CMs. Several pseudoclinical and random VMIMs were studied to determine the applicability of this new technique. A nine level boost map was also studied to investigate dosimetric and spatial resolution issues. Finally, clinical issues for this technique are discussed.

Waves in elastic semiconductors in a bias electric field

Certain aspects of bulk and surface wave propagation are studied in elastic semiconductors on the basis of a previously given phenomenological, nonlinear, rotationally invariant, thermodynamically admissible theory of deformable semiconductors. More precisely, the paper aims at exhibiting the dynamical couplings between various elastic modes and electric properties when an elastic semiconductor has been subjected to an intense bias electric field which breaks the “natural” symmetry and thereby induces couplings (electromechanical couplings, elasto-resistance, influence of initial charges) which would be ignored, had the theory been linear from the start. Special attention is devoted to the coupling of bulk elastic and charge waves for both longitudinal and orthogonal settings of the bias field and to alterations brought by conduction and induced electromechanical couplings on surface modes when the bias field is set orthogonal to the sagittal plane. Then the influence of conduction on the main properties (dispersion, attenuation, penetration depth) of Bleustein-Gulyaev waves is exhibited. The study is analytical, using small characteristic parameters to study complex couplings when necessary. Numerical estimates, however, allow one to judge of the relative influence of conduction as compared to the more usual dielectric case.

Coupled acoustic–optic modes in deformable ferroelectrics

Based on a fully Galilean electrodynamics of ferroelectric dielectric deformable bodies and the equations for weak fields linearized about an initial rigid-body ferroelectric state with hexagonal symmetry deduced in a previous paper (in which a symmetry breaking was exhibited), a rather complete study of coupled, dispersive, and attenuated bulk wave-propagation modes is presented. Apart from usual optic modes, resonance couplings and repulsion of branches for acoustic modes and polaritons are exhibited for various directions of propagation (longitudinal, orthogonal, and arbitrary settings of the bias polarization field). A birefringence effect for polaritons and an acoustical activity are placed in evidence. In addition to the analytical study which leans on the use of small coupling parameters in order to allow for a manageable discussion of the dispersion behavior for both nondissipative and dissipative processes, and to illustrate these theoretical results, numerical estimates and plots of the most interesting coupling effects are given for a test material such as ferroelectric barium titanate.