Original Line Research Articles

The Effect of Changing the Beam of an Ancient Ship’s Hull on Its Capacity, Stability, and Performance

Wooden ships on the shipwreck sites are usually only partially preserved, and reconstructing the original hull lines requires considerable effort. The shape of the hull has a direct effect on the ship’s capacity to carry cargo, as well as on its speed and stability. When reconstructing the hull lines, the incomplete nature of the archaeological remains results in the interpretation of the available data. The outcome, therefore, depends on the assumptions and decisions associated with the reconstruction process. This paper examines how the variation in a single parameter, namely, the beam, affects the performance of the vessel. Considering the availability of the model, the Kyrenia ship from the fourth/third century BC is used as a case study. The scope of this paper is to demonstrate and quantify the effect of beam variation on ancient ship performance, namely, the ship cargo capacity, stability, and resistance. Kyrenia ship was used as a study case based on hull lines proposed by Steffy in 1985. The aim is not to modify Steffy’s original reconstruction but to demonstrate that small deviations could significantly affect the performance of the vessel. In addition, an increase in the height of the ship’s sides is proposed as a possible solution to increase the load capacity of the ship. The opportunity to explore a whole set of trials and reconstructive variations with naval engineering software can deepen our understanding of ship performance, allowing us to improve our approach to reconstruction, too.

Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture.

Chinese hamster ovary (CHO) cell lines, derived as subclones from the original CHO cell line, are widely used hosts for current biopharmaceutical productions. Recently, a highly proliferative host cell line, CHO-MK, was established from the Chinese hamster ovary tissue. In this study, we assessed the fundamental culture characteristics and capabilities of CHO-MK cells for monoclonal antibody (mAb) production using specified chemically defined media. To achieve this, we established fed-batch cultures of model CHO-MK cells in shake flasks and ambr15 and 2 L bioreactors under various conditions. The mAb-producing CHO-MK cell line A produced 12.6g/L of antibody within 7days in the fed-batch culture using a 2 L bioreactor, with a seeding density of 1 × 106 cells/mL. This performance corresponded to a space-time yield of 1.80g/L/day, representing a productivity level that could be challengingly attained in fed-batch cultures using conventional CHO cells. In addition, when we subjected six different mAb-producing CHO-MK cell lines to fed-batch culture in the ambr15 bioreactor for 7days, the antibody production ranged between 5.1 and 10.8g/L, confirming that combining CHO-MK cells and specified media leads to enhanced versatility. These discoveries underscore that CHO-MK cells combined with specified media might represent a next-generation production platform, which could potentially respond to an increasing demand for antibody drugs, reducing production costs, and shortening antibody drug development times. This study is expected to serve as a benchmark for future production process development using CHO-MK cells.

The Wnt1-Cre2 transgene causes aberrant recombination in non-neural crest cell types.

The Wnt1-Cre2 driver, designed to address the effect of Wnt1 overactivation in the ventral neural tube in the original Wnt1-Cre line, was recently shown to have ectopic expression in the male germline. When crossed with a reporter mouse, we observed fluorescent protein expression in non-neural-crest cell types in the gut. Here, we characterize the pattern of Cre-mediated recombination in the Wnt1-Cre2 driver using three transgenic reporter lines. We find aberrant reporter activation in the gut endoderm in embryonic and postnatal timepoints, starting as early as E8.5. This pattern of recombination was independent of the age, sex, and type of reporter line used, with the Wnt1-Cre2 allele inherited from either sires or dams resulting in ectopic fluorescence in the intestinal epithelium. We also detect reporter activity in the ventral neural tube. However, expression in the neural crest and its derivatives remained consistent with previous studies. We further quantify differences in the non-specific recombination observed across reporter lines using flow cytometry. Interestingly, the penetrance of reporter activation between reporter lines was different, with R26R mTmG showing less ectopic activation than the R26R tdTom and R26R eYFP lines. Finally, we propose a potential mechanism whereby genes surrounding the Wnt1-Cre2 insertion site on mouse chromosome 2 contribute to its Wnt1-independent activation in the endoderm. Taken together, our results suggest that users should exercise caution when using the Wnt1-Cre2 driver line for neural crest studies in the mouse.

A Recursive Optimization Approach for Buffer Allocation in Large Production Lines

Buffer allocation is a critical issue in the design stage of manufacturing systems, as buffer capacities may have a great impact on system performance. In this paper, we consider the problem of minimizing the total buffer capacity of a flow line to achieve a desired production rate. A recursive optimization approach is proposed to solve the problem in large production lines. Instead of optimizing a long line directly, the proposed approach decomposes it into two sub-lines, optimizes them recursively, and combines their solutions to find the optimal buffer distribution of the original line. Two different recursive algorithms are developed and their performance is demonstrated by comparing them with a gradient search algorithm. The numerical results show that the recursive algorithms are almost as accurate as the gradient algorithm, but much more efficient, especially for large production lines.

Generation of CRISPR/Cas9 modified human iPSC line with correction of heterozygous mutation in exon 6 of the CaSR gene.

The calcium-sensing receptor (CaSR) gene encodes a cell membrane G protein-coupled receptor (GPCR) which has a key role in maintaining the extracellular Ca2+ homeostasis. We aimed at correcting the compound heterozygous mutation in the 6th [c.1656delA, p.I554SfsX73] and 7th [c.2217T > A, p.C739X] exons of the CASR gene which the original patient-derived iPSC line had. The mutation is associated with neonatal severe primary hyperparathyroidism of the patient. We generated and characterized a CRISP/Cas9-edited hiPSC line with the restored sequence in the sixth exon of the CASR gene, bearing only heterozygous mutation in the 7th exon. The results showed that the new genetically modified cell line has karyotype without abnormalities, typical hiPSCs morphology, characteristic expression of pluripotency markers, and ability to develop into three germ layers, and differentiates in chondrogenic, adipogenic, osteogenic directions. This new cell line will complement the existing pool of CaSR-mutated cell lines, a valuable resource for in-depth understanding of neonatal severe primary hyperparathyroidism. This will allow further exploration of the application of pharmacological drugs in the context of personalized medicine to correct Ca-homeostasis disorders.

Discovery of genes that positively affect biomass and stress associated traits in poplar.

Woody biomass serves as a renewable resource for various industries, including pulp and paper production, construction, biofuels, and electricity generation. However, the molecular mechanisms behind biomass traits are poorly understood, which significantly curtails the speed and efficiency of their improvement. We used activation tagging to discover genes that can positively affect tree biomass-associated traits. We generated and screened under greenhouse conditions a population of 2,700 independent activation tagging lines. A total of 761 lines, which had significantly and positively affected at least one biomass-associated trait, were discovered. The tag was positioned in the genome for forty lines which were affected in multiple traits and activation of proximal genes validated for a subset. For two lines we fully recapitulated the phenotype of the original lines through overexpression. Moreover, the overexpression led to more pronounced and additional improvements, not observed in the original lines. Importantly, the overexpression of a Fasciclin-like gene (PtaFLA10) and a Patatin-like gene (PtaPAT) was found to substantially improve biomass, with a 40% increase in dry-stem weight, and enhance drought tolerance, respectively. Additionally, PtaPAT overexpression increased cellulose content, which is crucial for biofuel production. Our work shows that the activation tagging approach applied even on a non-genome saturation scale in a poplar tree can be successfully used for the discovery of genes positively modify biomass productivity. Such dominant forward genetics approaches can aid in biotechnological manipulation of woody biomass traits and help unravel the functions and mechanisms of individual genes, gene families, and regulatory modules.

Development characteristics and hazard analysis of debris flow along the Emei to Mianning section of the Chengdu–Kunming Railway

Geological disasters in the Emei to Mianning section of the Chengdu–Kunming Railway located in southwestern China occur frequently, and many debris flow gullies are distributed along the line. Debris flows often cause damage to the railway and highway that threatens the safe operation of the original line and the double-track line. Along the railway, the debris flow outbreak risks are analyzed for 53 debris flow gullies using field survey, remote sensing interpretation, and numerical simulation. Their developmental characteristics were judged according to the code. The results are used for numerical simulation of debris flow to further determine the movement characteristics and the accumulation range of debris flow. The results are combined with high-definition satellite images to analyze the form of the railway passing from the mouth of the ditch and the simulation results to determine the impact on the safe operation of the Chengdu–Kunming Railway when the debris flow erupts. Finally, disaster prevention and mitigation projects are proposed, and the prevention and control effects of the proposed works are validated.

The Panflute Technique: Novel 3D-Printed Patient Specific Instrumentation to Guide Curved Intra-Articular Osteotomies for Tibial Plateau Malunions.

Background/Objectives: 3D patient-specific corrective osteotomies are optimized for use with oscillating saws, thereby rendering it incapable of executing curved osteotomies. The aim of this technical note is to introduce and evaluate the Panflute technique, which facilitates curved osteotomies with precise depth control for intra-articular corrective osteotomies in posttraumatic tibial plateau malunions. Methods: A 33-year-old male patient with an intra-articular malunion was treated one year after index surgery of a lateral split-depression tibial plateau fracture with the Panflute technique. The guide design allowed for multiple drill trajectories in a curved path, recreating the original fracture lines. Cylindrical drill tubes in the guide were tailored to match bone trajectory length. This resulted in a patient-specific Panflute-like design enabling precise depth control, safeguarding posterior neurovascular structures. Secondly, the recreated fragment was reduced with a reduction guide, applied to the plate in situ, to facilitate reposition using the plate as tool and reference. Results: The procedure went without technical drawbacks or surgical complications. Postoperative assessment showed that repositioning of the osteotomized articular fragment was performed accurately: pre- to postoperative translational corrections were 5.4 to 0.5 mm posterior displacement for AP deformity (x-axis); 2.9 to 1.0 mm lateral to medial reduction (y-axis); and 5.9 to 0.6 mm cranial-caudal correction (z-axis). Clinically, at 3 months, the fracture united, the patient regained full flexion, and valgus defect-laxity resolved. Conclusions: The presented Panflute-osteotomy guide allows for a pre-planned curved osteotomy. Additionally, for every drill trajectory, the depth could be controlled. The proposed method may expand our surgical armamentarium of patient-specific 3D techniques and solutions for complex intra-articular osteotomies.

Searching medieval human remains using ground penetrating radar: A case study in Venosa (Basilicata, Southern Italy)

Controlled forensic geophysical research involving GPR has proven to be a valuable resource, and the information gathered from these studies has been applied to forensic casework. The probability of detecting a grave for a longer postmortem interval differs with the soil type and the materials added to the grave with the body. In the studied case a detailed GPR survey was conducted in the Basilica della Trinità at Venosa a village located about 40 km north from Potenza (Basilicata, Italy).Unfortunately during the restoration works of the Basilica, there was a cement spill inside a sarcophagus containing human remains. The necessity to perform the genetic analysis of medieval human remains to reconstruct the distribution of the original line of descent of the Norman noble families aimed the need to understand whether or not there was a body inside the sarcophagus and, if so, its exact position.The radar profiles from this survey showed the clear amplitude contrast anomalies, emanated from the corpses. The strongest amplitude contrasts are observed at around 0.2–0.5 m depth which is consistent with the depth of the buried corp.

Clinical significance of analysis of long non-coding RNA PSMB8-AS1, MBNL1-AS1, and OLMALINC expression by polymerase chain reaction in non-small cell lung cancer

Rationale: Long non-coding RNAs (lncRNAs) influence tumor cell properties during the onset and progression of lung malignancies; however, their diagnostic and prognostic significance has not been determined. We have previously shown that when non-small cell lung cancer (NSCLC) cells acquire a more malignant phenotype (under the influence of macrophagal cytotoxic activity) compared to the original cell lines, the expression of several lncRNAs, in particular PSMB8-AS1, MBNL1-AS1, and OLMALINC, is altered compared to the original cell lines. Aim: A comparative analysis of lncRNAs PSMB8-AS1, MBNL1-AS1, and OLMALINC expression in tissue samples from lung tumors and conditionally normal areas of the lungs and an assessment of the lncRNAs clinical significance. Methods: We have analyzed surgical samples of the tumor and conditionally normal tissue from 16 patients with a verified diagnosis of NSCLC. The expression level of lncRNAs PSMB8-AS1, MBNL1-AS1 and OLMALINC was assessed by real-time polymerase chain reaction. To analyze the long-term treatment results and clinical significance of the studied genes, the patients were divided into two comparison groups depending on the relative level of lncRNAs expression (above or below the median). Results: The expression of lncRNAs PSMB8-AS1, MBNL1-AS1 and OLMALINC in the lung tumor tissue was significantly reduced compared to the conditionally normal tissues (p = 0.0034; p = 0.002 and p = 0.0172, respectively). Analysis of the association between the expression of these lncRNAs with clinical and morphological characteristics, such as disease stage, tumor size, presence of regional and distant metastases was unable to identify any regular patterns. The expression of lncRNAs PSMB8-AS1, MBNL1-AS1 and OLMALINC was not a significant prognostic factor (p = 0.364; p = 0.759 and p = 0.184, respectively). However in the case of high OLMALINC and PSMB8-AS1 expression, median survival was 47 months, while in the case of their low expression, median survival was not achieved during the follow-up. The expression of lncRNA PSMB8-AS1 in NSCLC tumors positively correlated with the expression of lncRNA OLMALINC (r = 0.680, p = 0.007), which may indicate their functional interplay or the presence of common regulatory mechanisms. Conclusion: The NSCLC tumors demonstrated aberrant expression of PSMB8-AS1, MBNL1-AS1, and OLMALINC lncRNAs. A more detailed study of their expression in various cell types and their regulatory role would allow for validation of new therapeutic targets in NSCLC, as well as for development of alternative therapies.

Geophysical archaeology for the study of complex historical-period burial spaces: An example from the Fort Lewis military cemetery, Colorado

Geophysical methods have long been used to locate and map unmarked graves in cemeteries and burial spaces. Despite their prevalence, geophysical cemetery surveys can be extremely complex, particularly when dealing with historical-period cemeteries. Not only are there interpretive challenges from site formation processes and noncultural features, but there are also cultural challenges when seeking to study a cemetery within its historical context. Geophysical archaeology is a specialization that can guide this type of work by underscoring the cultural context for geophysical data. Beyond simply locating unmarked graves or other “anomalies,” geophysical archaeology emphasizes increasing the interpretive ability of geophysical data by situating these surveys in cultural theory. Using the example of the Fort Lewis military cemetery, which also served the Fort Lewis Indian Boarding School, in southwest Colorado, this paper highlights how geophysical archaeology was used to identify specific features about the cemetery, including the original fence line, number of likely graves, and two different burial contexts (casketed burials and exhumed graves).

Effect of mutations in the <i>Sym7</i>, <i>Sym19</i> and <i>Sym34</i> genes on the interaction of pea (<i>Pisum sativum</i> L.) with the arbuscular mycorrhizal fungus <i>Rhizophagus irregularis</i>

BACKGROUND: Within the pea (Pisum sativum L.) species, genotypes with high and low responsiveness to inoculation with arbuscular mycorrhizal fungi can be distinguished. AIM: The aim of this study was to test the hypothesis that pea responsiveness to arbuscular mycorrhizal fungi inoculation may be inversely correlated with root colonization levels. MATERIALS AND METHODS: The wild-type line Finale with low responsiveness to arbuscular mycorrhizal fungi inoculation and symbiotic mutants obtained on its basis were used. Plants were grown under controlled climatic conditions with a deficiency of available phosphorus; the fungus Rhizophagus irregularis was used for inoculation. Parameters of plant growth and development of reproductive organs were determined 52 and 71 days after inoculation, which corresponded to the flowering and pod filling stages, respectively. RESULTS: All mutant lines under conditions without inoculation had generally reduced parameters compared to the original line Finale. Inoculation led to a decrease in many parameters in the line Finale. Mutations in the Sym7 and Sym34 genes, which led to a decrease or delay in the start of mycorrhization, respectively, contributed to the manifestation of a positive plant response to inoculation. The mutant in the Sym19 gene almost completely lacked intrartadical colonization, while inoculation had no effect on the growth and development of above-ground organs. CONCLUSIONS: The study results support the idea that reducing mycorrhization levels can have a positive effect on pea plants.

Enhancing Photoswitchable Wetting Properties of Hydrophobic Porous Spiropyran Copolymer Surfaces Through Surface Roughness Engineering

AbstractSurfaces with photoswitchable wettability are of great interest for various applications such as smart coatings or liquid condensation. The photochromic ring opening reaction of spiropyran (SP) to merocyanine (MC) implies a high dipole moment change, making it interesting for photo‐controlled wetting properties. In addition to the material chemistry, surface wettability is influenced by the surface topography. Porous SP copolymers with various micro‐/nanostructures, that is, different submicron roughness, are fabricated via polymerization‐induced phase separation. The influence of the surface topography on the photoswitchable wetting properties is studied. Surfaces with arithmetic mean roughness (Sa) below 150 nm exhibited a maximum static contact angle (SCA) photoswitch up to 16° from 124 ± 6° to 108 ± 4° upon UV exposure. While superhydrophobic surfaces with higher Sa (157 – 608 nm) showed an insignificant SCA switch. The latter surfaces have a SCA above 150° and low CA hysteresis indicating small and insufficient contact with SP/MC surface asperities for the switch. With the optimized surfaces, photo‐controlled water condensation is studied on microscale and showed that the condensate droplets merged faster, and formed larger droplets pinned to the original contact lines on surfaces switched to the less hydrophobic state.

Interfacial microstructure evolution and mechanical response of TC19/Ti150 dissimilar joints obtained by diffusion bonding

The structure made by dissimilar titanium alloys can meet the differentiated requirements of performance for different parts, fully exerting their performance advantages during operation respectively, which holds significant technological and economic value. Here, we used diffusion bonding (DB) method to connect TC19 and Ti150 dissimilar titanium alloys in the temperature range of 810 °C–900 °C, and investigated the morphology, forming mechanism, deformation behavior and mechanical properties of the joints. The study showed that the interfacial voids disappeared with the increase of temperature, and the interfacial characteristics gradually evolved from the initial straight shape to a curved shape. The original bond line transformed into βTC19/αTi150 phase boundaries (PBs) and high-angle grain boundaries (HAGBs) formed by αTC19 and αTi150. No harmful intermetallic compounds were formed on the interface. The joint formation can be considered as a combination of three behaviors: plastic deformation, elemental diffusion and interfacial grain boundary migration (IGBM). In addition, it was observed that dislocation slip was the main deformation behavior of the joints. The dislocations generated in the both matrices were obviously accumulated at the interface during tensile deformation of the specimen, and the bonding interface hindered the dislocation transfer between two substrates. The room temperature uniaxial tensile results exhibited that the joints bonded at 810 °C and 840 °C were broken at the interface, showing poor mechanical properties caused by holes and straight interface. And yet the joints bonded at 870 °C and 900 °C occurred fracture on the Ti150 matrix. The strength of joints was comparable to that of the Ti150 matrix, indicating that the two dissimilar titanium alloys achieved a reliable bonding. This work provides valuable inspiration for the diffusion bonding process of titanium alloys with different microstructures.

MvGraphDTA: multi-view-based graph deep model for drug-target affinity prediction by introducing the graphs and line graphs

BackgroundAccurately identifying drug-target affinity (DTA) plays a pivotal role in drug screening, design, and repurposing in pharmaceutical industry. It not only reduces the time, labor, and economic costs associated with biological experiments but also expedites drug development process. However, achieving the desired level of computational accuracy for DTA identification methods remains a significant challenge.ResultsWe proposed a novel multi-view-based graph deep model known as MvGraphDTA for DTA prediction. MvGraphDTA employed a graph convolutional network (GCN) to extract the structural features from original graphs of drugs and targets, respectively. It went a step further by constructing line graphs with edges as vertices based on original graphs of drugs and targets. GCN was also used to extract the relationship features within their line graphs. To enhance the complementarity between the extracted features from original graphs and line graphs, MvGraphDTA fused the extracted multi-view features of drugs and targets, respectively. Finally, these fused features were concatenated and passed through a fully connected (FC) network to predict DTA.ConclusionsDuring the experiments, we performed data augmentation on all the training sets used. Experimental results showed that MvGraphDTA outperformed the competitive state-of-the-art methods on benchmark datasets for DTA prediction. Additionally, we evaluated the universality and generalization performance of MvGraphDTA on additional datasets. Experimental outcomes revealed that MvGraphDTA exhibited good universality and generalization capability, making it a reliable tool for drug-target interaction prediction.

Image restoration for digital line drawings using line masks

The restoration of digital images holds practical significance due to the fact that degradation of digital image data on the internet is common. State-of-the-art image restoration methods usually employ end-to-end trained networks. However, we argue that a network trained with diverse image pairs is not optimal for restoring line drawings which have extensive plain backgrounds. We propose a line-drawing restoration framework which takes a restoration neural network as backbone and processes an input degraded line drawing in two steps. First, a proposed mask-predicting network predicts a line mask which indicates the possible location of foreground and background in the potential original line drawing. Next, we feed the degraded input line drawing together with the predicted line mask into the backbone restoration network. The traditional L1 loss for the backbone restoration network is substituted with a masked Mean Square Error (MSE) loss. We test our framework on two classical image restoration tasks: JPEG restoration and super-resolution, and experiments demonstrate that our framework can achieve better quantitative and visual results in most cases.

Graphic Novel Adaptation as a Subgenre (Franco- and Anglophone Texts)

The objective of this study is to define the graphic novel adaptation as a distinct subgenre of the graphic novel and to develop a cohesive methodology for its examination. The research material comprises Franco- and Anglophone graphic novel adaptations published since the early 2000s. It is noted that this subgenre has become one of the most popular forms of mass literature, characteristic of the twenty-first century as an era of visual culture. Based on a review of theoretical studies, the fundamental characteristics of graphic novel adaptations are articulated. An analysis of works from the past decade demonstrates the evolution of the subgenre under consideration. Specifically, it is observed that (1) the original narrative line is modified through the incorporation of themes and issues relevant to twenty-first-century individuals; (2) there is an increased complexity in character development, structure, and chronotope; (3) a philosophical dimension is foregrounded. It is shown that graphic novel adaptations can be categorized into two types based on target audience: mass (“low brow”) and intellectual (“high brow”). The “low brow” type primarily aims to popularize the reading of classic texts, while the “high brow” type represents an authorial interpretation of a well-known literary work. The intellectual form (“high brow”) has become increasingly prevalent since the 2010s.

A complementary approach to quantify the basic GSI chart considering scale effect on rock structure

Geological strength index (GSI) has been widely used as an input parameter in predicting the strength and deformation properties of rock masses. This study derived a series of equations to satisfy the original GSI lines on the basic GSI chart. Two axes ranging from 0 to 100 were employed for surface conditions of the discontinuities and the structure of rock mass, which are independent of the input parameters. The derived equations can analyze GSI values ranging from 0 to 100 within ±5% error. The engineering dimensions (EDs) such as the slope height, tunnel width, and foundation width were used together with representative elementary volume (REV) in jointed rock mass to define scale factor (sf) from 0.2 to 1 in evaluating the rock mass structure including joint pattern. The transformation of GSI into a scale-dependent parameter based on engineering scale addresses a crucial requirement in various engineering applications. The improvements proposed in this study were applied to a real slope which was close to the time of failure. The results of stability assessments show that the new proposals have sufficient capability to define rock mass quality considering EDs.

Quantifying allele-specific CRISPR editing activity with CRISPECTOR2.0.

Off-target effects present a significant impediment to the safe and efficient use of CRISPR-Casgenome editing. Since off-target activity is influenced by the genomic sequence, the presence of sequence variants leads to varying on- and off-target profiles among different alleles or individuals. However, a reliable tool that quantifies genome editing activity in an allelic context is not available. Here, we introduce CRISPECTOR2.0, an extended version of our previously published software tool CRISPECTOR, with an allele-specific editing activity quantification option. CRISPECTOR2.0 enables reference-free, allele-aware, precise quantification of on- and off-target activity, by using de novo sample-specific single nucleotide variant (SNV) detection and statistical-based allele-calling algorithms. We demonstrate CRISPECTOR2.0 efficacy in analyzing samples containing multiple alleles and quantifying allele-specific editing activity, using data from diverse cell types, including primary human cells, plants, and an original extensive human cell line database. We identified instances where an SNV induced changes in the protospacer adjacent motif sequence, resulting in allele-specific editing. Intriguingly, differential allelic editing was also observed in regions carrying distal SNVs, hinting at the involvement of additional epigenetic factors. Our findings highlight the importance of allele-specific editing measurement as a milestone in the adaptation of efficient, accurate, and safe personalized genome editing.

GOOD MOANING! THE HUMOROUS ALTERATIONS AND TRANSLATION MECHANISMS IN THE LINES OF ‘ALLo ‘ALLo!’S OFFICER CRABTREE

Officer Crabtree is a memorable character from the British sitcom ‘Allo ‘Allo!, char- acterized by his pseudo-English speech. The ways in which his language deviates from the stan- dard English bring about a very humorous effect. This paper introduces the main tenets of the incongruity theory and the main types of wordplay through which Crabtree’s speech is delivered, before defining the relevant morphological and morphosyntactic shifts which dominate his lines in the series. Next, the paper collects all of his original lines from the show, analyzes them for different morphological changes and classifies them accordingly, while the Serbian translation of his lines is also taken into account and analyzed for further morphological and morphosyntactic shifts. Additionally, the direct translation mechanisms from English to Serbian are analyzed and classified as well. The results are first expressed in terms of percentage, and then compared be- tween the two languages, with the aim to discover which of the presented alterations dominate in creating the comical effect for the audience.