Original High-resolution Image Research Articles

Rectified Binary Network for Single-Image Super-Resolution.

Binary neural network (BNN) is an effective approach to reduce the memory usage and the computational complexity of full-precision convolutional neural networks (CNNs), which has been widely used in the field of deep learning. However, there are different properties between BNNs and real-valued models, making it difficult to draw on the experience of CNN composition to develop BNN. In this article, we study the application of binary network to the single-image super-resolution (SISR) task in which the network is trained for restoring original high-resolution (HR) images. Generally, the distribution of features in the network for SISR is more complex than those in recognition models for preserving the abundant image information, e.g., texture, color, and details. To enhance the representation ability of BNN, we explore a novel activation-rectified inference (ARI) module that achieves a more complete representation of features by combining observations from different quantitative perspectives. The activations are divided into several parts with different quantification intervals and are inferred independently. This allows the binary activations to retain more image detail and yield finer inference. In addition, we further propose an adaptive approximation estimator (AAE) for gradually learning the accurate gradient estimation interval in each layer to alleviate the optimization difficulty. Experiments conducted on several benchmarks show that our approach is able to learn a binary SISR model with superior performance over the state-of-the-art methods. The code will be released at https://github.com/jwxintt/Rectified-BSR.

Attention-Based MultiOffset Deep Learning Reconstruction of Chemical Exchange Saturation Transfer (AMO-CEST) MRI.

One challenge of chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) is the long scan time due to multiple acquisitions of images at different saturation frequency offsets. k-space under-sampling strategy is commonly used to accelerate MRI acquisition, while this could introduce artifacts and reduce signal-to-noise ratio (SNR). To accelerate CEST-MRI acquisition while maintaining suitable image quality, we proposed an attention-based multioffset deep learning reconstruction network (AMO-CEST) with a multiple radial k-space sampling strategy for CEST-MRI. The AMO-CEST also contains dilated convolution to enlarge the receptive field and data consistency module to preserve the sampled k-space data. We evaluated the proposed method on a mouse brain dataset containing 5760 CEST images acquired at a pre-clinical 3 T MRI scanner. Quantitative results demonstrated that AMO-CEST showed obvious improvement over zero-filling method with a PSNR enhancement of 11 dB, a SSIM enhancement of 0.15, and a NMSE decrease of [Formula: see text] in three acquisition orientations. Compared with other deep learning-based models, AMO-CEST showed visual and quantitative improvements in images from three different orientations. We also extracted molecular contrast maps, including the amide proton transfer (APT) and the relayed nuclear Overhauser enhancement (rNOE). The results demonstrated that the CEST contrast maps derived from the CEST images of AMO-CEST were comparable to those derived from the original high-resolution CEST images. The proposed AMO-CEST can efficiently reconstruct high-quality CEST images from under-sampled k-space data and thus has the potential to accelerate CEST-MRI acquisition.

Super-resolution reconstruction of 3D digital rocks by deep neural networks

Digital rock technology provides valuable insights into the pore structure and fluid flow properties of geoenergy resources. Artificial intelligence technology in vision and image processing, especially the image super-resolution, has great potential for digital rock reconstruction and resolution enhancement. However, the analyzed core samples are typically sandstones/carbonates in micro-scale resolutions and in two-dimensional (2D) space, whereas the shale rocks in nano-scale resolutions for unconventional resources or three-dimensional (3D) digital cores are rarely investigated. Additionally, previous studies primarily emphasized image quality from a computer vision perspective, with little consideration given to estimating physical properties of digital rocks using super-resolution techniques.This study presents a very deep super-resolution (VDSR) algorithm, specifically designed to generate high-resolution 3D digital rock images, for nano-scale shale matrix and micro-scale hydraulic fractures. We compare both image quality and permeability accuracy between the original high-resolution images and the super-resolution images reconstructed by the proposed method. The results reveal that the reconstructed images using the proposed method closely resemble the actual images, and effectively reduce errors in permeability computations. This study highlights the applicability of the proposed VDSR algorithm in establishing the detailed structures of 3D nano-scale shale matrix and hydraulic fractured rocks, thus advancing super-resolution techniques in digital core analysis for geoenergy resources development.

Rethinking Prior-Guided Face Super-Resolution: A New Paradigm With Facial Component Prior.

Recently, facial priors (e.g., facial parsing maps and facial landmarks) have been widely employed in prior-guided face super-resolution (FSR) because it provides the location of facial components and facial structure information, and helps predict the missing high-frequency (HF) information. However, most existing approaches suffer from two shortcomings: 1) the extracted facial priors are inaccurate since they are extracted from low-resolution (LR) or low-quality super-resolved (SR) face images and 2) they only consider embedding facial priors into the reconstruction process from LR to SR face images, thus failing to explore facial priors to generate LR face image. In this article, we propose a novel pre-prior guided approach that extracts facial prior information from original high-resolution (HR) face images and embeds them into LR ones to obtain HF information-rich LR face images, thereby improving the performance of face reconstruction. Specifically, a novel component hybrid method is proposed, which fuses HR facial components and LR facial background to generate new LR face images (namely, LRmix) via facial parsing maps extracted from HR face images. Furthermore, we design a component hybrid network (CHNet) that learns the LR to LRmix mapping function to ensure that the LRmix can be obtained from LR face images in testing and real-world datasets. Experimental results show that our proposed scheme significantly improves the reconstruction performance for FSR.

Enhancing Knee MR Image Clarity through Image Domain Super-Resolution Reconstruction.

This study introduces a hybrid analytical super-resolution (SR) pipeline aimed at enhancing the resolution of medical magnetic resonance imaging (MRI) scans. The primary objective is to overcome the limitations of clinical MRI resolution without the need for additional expensive hardware. The proposed pipeline involves three key steps: pre-processing to re-slice and register the image stacks; SR reconstruction to combine information from three orthogonal image stacks to generate a high-resolution image stack; and post-processing using an artefact reduction convolutional neural network (ARCNN) to reduce the block artefacts introduced during SR reconstruction. The workflow was validated on a dataset of six knee MRIs obtained at high resolution using various sequences. Quantitative analysis of the method revealed promising results, showing an average mean error of 1.40 ± 2.22% in voxel intensities between the SR denoised images and the original high-resolution images. Qualitatively, the method improved out-of-plane resolution while preserving in-plane image quality. The hybrid SR pipeline also displayed robustness across different MRI sequences, demonstrating potential for clinical application in orthopaedics and beyond. Although computationally intensive, this method offers a viable alternative to costly hardware upgrades and holds promise for improving diagnostic accuracy and generating more anatomically accurate models of the human body.

MT-Det: A novel fast object detector of maize tassel from high-resolution imagery using single level feature

Accurately and quickly detecting maize tassels is crucial for successful maize breeding and seed production. However, the complex planting environment in the field, including unsynchronized growth stages, cluttered backgrounds, severe occlusions, and varying tassel sizes and shapes, presents a challenge for detection methods. Current methods either use mainstream object detectors off-the-shelf or employ shallow adaptations on the modular level, lacking a systematic design that balances both speed and accuracy. Furthermore, most studies only report evaluation results on sliced image patches instead of original high-resolution images, which can offer limited guidance for large-scale crop management. In this study, we propose a novel one-stage anchor-free maize tassel detector (MT-Det), which is based on a single level feature and designed to be simple yet effective. Extensive comparisons with both one-level counterparts and feature pyramid detectors demonstrate that MT-Det outperforms them in both detection accuracy and inference speed. To address the issue of significant accuracy drop when directly inferring on high-resolution images, we introduce a bundle of slicing-aided hyper inference technologies to our proposed MT-Det, which results in a 13% and 38% improvement of mean average precision (mAP) on proximal and unmanned aerial vehicle (UAV) high-resolution images, respectively. We believe that MT-Det provides a promising high-throughput solution for accurate and efficient detection and counting of maize tassels in real-world field conditions.

Adaptive Filter in Single Image SRGAN

Abstract: SRGAN is a generative adversarial network for super-resolution of a single picture. In this paper an adaptive filter in single image SRGAN which is a Super-Resolution Generative Adversarial Network (SRGAN), is a deep learning-based approach that is used to generate high-resolution images from low-resolution ones. The SRGAN model is based on the Generative Adversarial Network (GAN) architecture, which consists of two deep neural networks: a generator network and a discriminator network. The generator network takes a low-resolution image as input and tries to generate a high-resolution image that is similar to the original high-resolution image. The discriminator network, on the other hand, tries to distinguish between the high-resolution images generated by the generator network and the original high-resolution images. The SRGAN model has been shown to be very effective in generating high-quality, realistic images from low-resolution inputs, and it has been applied in various applications, including image and video upscaling, medical imaging, and satellite imaging

A Secure Cooperative Transmission of Image Super-Resolution in Wireless Relay Networks

The image transmission over wireless media experiences not only unavailable performance loss caused by the environment and hardware issues, but also information leakage to eavesdroppers who can overhear and attempt to recover the images. This paper proposes a secure cooperative relaying (SCR) protocol for the image communications in wireless relay networks (WRNs) where Alice sends high-resolution (HR) images to Bob with the assistance of a relaying user named Relay, and in the presence of an eavesdropper named Eve. In order to enhance the security of the image communications, random linear network coding (RLNC) is employed at both Alice and Relay to conceal the original images from Eve with RLNC coefficient matrices and reference images in the shared image datastore. Furthermore, the original HR images are downscaled at Alice to save transmission bandwidth and image super-resolution (ISR) is adopted at Bob due to its capability to recover the HR images from their low-resolution (LR) version, while still maintaining the image quality. In the proposed SCR protocol, Bob can decode both the original images transmitted from Alice over the direct link and the images forwarded by Relay over the relaying links. Simulation results show that the SCR protocol achieves a considerably higher performance at Bob than at Eve since Eve does not know the coefficient matrices and reference images used at Alice and Relay for the RLNC. The SCR protocol is also shown to outperform the counterpart secure direct transmission protocol without the relaying links and secure relaying transmission without the direct link. Additionally, an increased scaling factor can save the transmission bandwidth for a slight change in the image quality. Moreover, the impacts of direct, relaying and wiretap links are evaluated, verifying the effectiveness of the SCR protocol with the employment of Relay to assist the image communications between Alice and Bob in the WRNs.

IESRGAN: Enhanced U-Net Structured Generative Adversarial Network for Remote Sensing Image Super-Resolution Reconstruction

With the continuous development of modern remote sensing satellite technology, high-resolution (HR) remote sensing image data have gradually become widely used. However, due to the vastness of areas that need to be monitored and the difficulty in obtaining HR images, most monitoring projects still rely on low-resolution (LR) data for the regions being monitored. The emergence of remote sensing image super-resolution (SR) reconstruction technology effectively compensates for the lack of original HR images. This paper proposes an Improved Enhanced Super-Resolution Generative Adversarial Network (IESRGAN) based on an enhanced U-Net structure for a 4× scale detail reconstruction of LR images using NaSC-TG2 remote sensing images. In this method, in-depth research has been performed and consequent improvements have been made to the generator and discriminator within the GAN network. Specifically, before introducing Residual-in-Residual Dense Blocks (RRDB), in the proposed method, input images are subjected to reflective padding to enhance edge information. Meanwhile, a U-Net structure is adopted for the discriminator, incorporating spectral normalization to focus on semantic and structural changes between real and fake images, thereby improving generated image quality and GAN performance. To evaluate the effectiveness and generalization ability of our proposed model, experiments were conducted on multiple real-world remote sensing image datasets. Experimental results demonstrate that IESRGAN exhibits strong generalization capabilities while delivering outstanding performance in terms of PSNR, SSIM, and LPIPS image evaluation metrics.

Age and gender classification with bone images using deep learning algorithms

In paediatrics, bone age is a crucial indicator of how a child's skeleton is developing. They have had great success ever since the creation of deep learning (DL)-based bone age prediction tools. Deep features learning, however, has a significant computing overhead problem. Deep convolution layers are used in this technique to learn representative features in the small yet useful regions that are extracted for feature learning. This work suggests using an extreme learning machine algorithm as the fundamental architecture in the final bone age assessment study to realise the rapid computation speed and feature interaction. The viability and efficacy of the suggested strategy have been verified by experiments using data that is openly accessible. The suggested model is explicitly trained using a cutting-edge end-to-end learning architecture employing bone scans to extract the most discriminative patches from the original high-resolution image. The bone picture is the foundation of the procedure. Our main objective is to categorize individuals by age using convolution neural network (CNN) classification models, such as the Xception and Mobile Net models. As a result, we have achieved results that are 90% and 94% accurate in classifying people by age using CNN models.

Lucy Richardson and Mean Modified Wiener Filter for Construction of Super-Resolution Image

The ultimate goal of the Super-Resolution (SR) technique is to generate the High-Resolution (HR) image by combining the corresponding images with Low-Resolution (LR), which is utilized for different applications such as surveillance, remote sensing, medical diagnosis, etc. The original HR image may be corrupted due to various causes such as warping, blurring, and noise addition. SR image reconstruction methods are frequently plagued by obtrusive restorative artifacts such as noise, stair casing effect, and blurring. Thus, striking a balance between smoothness and edge retention is never easy. By enhancing the visual information and autonomous machine perception, this work presented research to improve the effectiveness of SR image reconstruction The reference image is obtained from DIV2K and BSD 100 dataset, these reference LR image is converted as composed LR image using the proposed Lucy Richardson and Modified Mean Wiener (LR-MMWF) Filters. The possessed LR image is provided as input for the stage of bicubic interpolation. Afterward, the initial HR image is obtained as output from the interpolation stage which is given as input for the SR model consisting of fidelity term to decrease residual between the projected HR image and detected LR image. At last, a model based on Bilateral Total Variation (BTV) prior is utilized to improve the stability of the HR image by refining the quality of the image. The results obtained from the performance analysis show that the proposed LR-MMW filter attained better PSNR and Structural Similarity (SSIM) than the existing filters. The results obtained from the experiments show that the proposed LR-MMW filter achieved better performance and provides a higher PSNR value of 31.65dB whereas the Filter-Net and 1D,2D CNN filter achieved PSNR values of 28.95dB and 31.63dB respectively.

Scale-Arbitrary Invertible Image Downscaling.

Conventional social media platforms usually downscale high-resolution (HR) images to restrict their resolution to a specific size for saving transmission/storage cost, which makes those visual details inaccessible to other users. To bypass this obstacle, recent invertible image downscaling methods jointly model the downscaling/upscaling problems and achieve impressive performance. However, they only consider fixed integer scale factors and may be inapplicable to generic downscaling tasks towards resolution restriction as posed by social media platforms. In this paper, we propose an effective and universal Scale-Arbitrary Invertible Image Downscaling Network (AIDN), to downscale HR images with arbitrary scale factors in an invertible manner. Particularly, the HR information is embedded in the downscaled low-resolution (LR) counterparts in a nearly imperceptible form such that our AIDN can further restore the original HR images solely from the LR images. The key to supporting arbitrary scale factors is our proposed Conditional Resampling Module (CRM) that conditions the downscaling/upscaling kernels and sampling locations on both scale factors and image content. Extensive experimental results demonstrate that our AIDN achieves top performance for invertible downscaling with both arbitrary integer and non-integer scale factors. Also, both quantitative and qualitative evaluations show our AIDN is robust to the lossy image compression standard. The source code and trained models will be publicly available upon publication.

Image interpolation by direct cross downsampling

Direct downsampling (DD) based interpolation system can be used as an encoder-decoder framework for secure data transmission, while DD is often used as a data compression way and interpolation is used for decompression. In this paper, we propose a new direct cross downsampling (DCD) algorithm, which has the same function as DD. Compared to DD, the DCD has three main differences from the angle of image interpolation: 1) DCD can use the nearest neighboring pixels for image interpolation to effectively reduce the interpolation error; 2) More pixels can be interpolated in the first step in those two-step interpolation methods with the help of the DCD; 3) The DCD preserves the information of each column or each row in the original high-resolution (HR) image while DD discards the even columns and rows. On the basis of the proposed DCD algorithm, we further propose four DCD-based interpolation methods: DCD-based bilinear interpolation, DCD-based bicubic interpolation, DCD-based content-adaptive interpolation (CAI) and DCD-based edge-oriented interpolation (EOI). By comparing the proposed four DCD-based image interpolation ways with those corresponding DD-based interpolation strategies in both experimental and analytical ways, we have proved the superiority of the DCD algorithm. Furthermore, we compared the proposed DCD-based EOI with three previous excellent DD-based interpolation works and experimental results demonstrate that the proposed DCD-based EOI has the best performance on PSNR and SSIM.

Lung Field Segmentation in Chest X-ray Images Using Superpixel Resizing and Encoder-Decoder Segmentation Networks.

Lung segmentation of chest X-ray (CXR) images is a fundamental step in many diagnostic applications. Most lung field segmentation methods reduce the image size to speed up the subsequent processing time. Then, the low-resolution result is upsampled to the original high-resolution image. Nevertheless, the image boundaries become blurred after the downsampling and upsampling steps. It is necessary to alleviate blurred boundaries during downsampling and upsampling. In this paper, we incorporate the lung field segmentation with the superpixel resizing framework to achieve the goal. The superpixel resizing framework upsamples the segmentation results based on the superpixel boundary information obtained from the downsampling process. Using this method, not only can the computation time of high-resolution medical image segmentation be reduced, but also the quality of the segmentation results can be preserved. We evaluate the proposed method on JSRT, LIDC-IDRI, and ANH datasets. The experimental results show that the proposed superpixel resizing framework outperforms other traditional image resizing methods. Furthermore, combining the segmentation network and the superpixel resizing framework, the proposed method achieves better results with an average time score of 4.6 s on CPU and 0.02 s on GPU.

Unsupervised Super Resolution Network for Hyperspectral Histologic Imaging.

Hyperspectral imaging (HSI) has many advantages in microscopic applications, including high sensitivity and specificity for cancer detection on histological slides. However, acquiring hyperspectral images of a whole slide with a high image resolution and a high image quality can take a long scanning time and require a very large data storage. One potential solution is to acquire and save low-resolution hyperspectral images and reconstruct the high-resolution ones only when needed. The purpose of this study is to develop a simple yet effective unsupervised super resolution network for hyperspectral histologic imaging with the guidance of RGB digital histology images. High-resolution hyperspectral images of hemoxylin & eosin (H&E) stained slides were obtained at 10× magnification and down-sampled 2×, 4×, and 5× to generate low-resolution hyperspectral data. High-resolution digital histologic RGB images of the same field of view (FOV) were cropped and registered to the corresponding high-resolution hyperspectral images. A neural network based on a modified U-Net architecture, which takes the low-resolution hyperspectral images and high-resolution RGB images as inputs, was trained with unsupervised methods to output high-resolution hyperspectral data. The generated high-resolution hyperspectral images have similar spectral signatures and improved image contrast than the original high-resolution hyperspectral images, which indicates that the super resolution network with RGB guidance can improve the image quality. The proposed method can reduce the acquisition time and save storage space taken up by hyperspectral images without compromising image quality, which will potentially promote the use of hyperspectral imaging technology in digital pathology and many other clinical applications.

RRSGAN: Reference-Based Super-Resolution for Remote Sensing Image

Remote sensing image super-resolution (SR) plays an important role by supplementing the lack of original high-resolution (HR) images in the study scenarios of large spatial areas or long time series. However, due to the lack of imagery information in low-resolution (LR) images, single-image super-resolution (SISR) is an inherently ill-posed problem. Especially, it is difficult to reconstruct the fine textures of HR images at large upscaling factors (e.g., four times). In this work, based on Google Earth HR images, we explore the potential of the reference-based super-resolution (RefSR) method on remote sensing images, utilizing rich texture information from HR reference (Ref) images to reconstruct the details in LR images. This method can use existing HR images to help reconstruct the LR images of long time series or a specific time. We build a reference-based remote sensing SR data set (RRSSRD). Furthermore, by adopting the generative adversarial network (GAN), we propose a novel end-to-end reference-based remote sensing GAN (RRSGAN) for SR. RRSGAN can extract the Ref features and align them to the LR features. Eventually, the texture information in the Ref features can be transferred to the reconstructed HR images. In contrast to the existing RefSR methods, we propose a gradient-assisted feature alignment method that adopts the deformable convolutions to align the Ref and LR features and a relevance attention module (RAM) to improve the robustness of the model in different scenarios (e.g., land cover changes and cloud coverage). The experimental results demonstrate that RRSGAN is robust and outperforms the state-of-the-art SISR and RefSR methods in both quantitative evaluation and visual results, which indicates the great potential of the RefSR method for remote sensing tasks. Our code and data are available at <uri xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">https://github.com/dongrunmin/RRSGAN</uri> .

Hierarchical object detection for very high-resolution satellite images

Object detection from satellite images is challenging and either computationally expensive or labor intense. Satellite images often cover large areas of more than 10km×10km. They include objects of different scales, which makes it hard to detect all of them at the same image resolution. Considering that airplanes are usually located in airports, ships are often distributed in ports and sea areas, and that oil depots are typically found close to airports or ports, we propose a new hierarchical object detection framework for very high-resolution satellite images. Our framework prescribes two stages: (1) detecting airports and ports in down-sampled satellite images and (2) mapping the detected object back to the original high-resolution satellite images for detecting the smaller objects near them. In order to improve the efficiency of object detection, we further propose a contextual information based deep feature extraction approach for both of the hierarchical detection steps, as well as an inclined bounding box based arbitrarily-oriented object location mechanism suitable especially for the smaller objects. Comprehensive experiments on a public dataset and two self-assembled datasets (which we made publicly available) show the superior performance of our method compared to standalone state-of-the-art object detectors.

Selective region enlargement network for fast object detection in high resolution images

Detecting objects of varied sizes in high resolution images is difficult due to the challenges of high memory requirement and huge computation burden. Existing state-of-the-art detectors perform well on low resolution images. However, its performance is greatly limited on high resolution images. In this paper, we propose a selective region enlargement network, called SRENet, which significantly reduces processing time and memory requirement while remaining high detection accuracy. The proposed SRENet does not need to conduct detection on original high resolution images but only needs to conduct detection on down-sampled images and some zoom-in regions selected from high resolution images. SRENet first conducts coarse detection on a low resolution image, and then sequentially selects promising regions that are expected to be analyzed at a higher resolution. Specifically, SRENet is built upon Deep Q-learning Network (DQN) and it outputs an action-reward map. The value of the reward map indicates the possibility that the action can improve detection accuracy. The region selected by the action with the maximum reward value will be analyzed further at a higher resolution. Extensive experiments are conducted to demonstrate SRENet on two challenging datasets obtaining high resolution images. Experimental results show that SRENet achieves state-of-the-art detection performance with high efficiency.

Super Resolution of Magnetic Resonance Images.

In this work, novel denoising and super resolution (SR) approaches for magnetic resonance (MR) images are addressed, and are integrated in a unified framework, which do not require example low resolution (LR)/high resolution (HR)/cross-modality/noise-free images and prior information of noise–noise variance. The proposed method categorizes the patches as either smooth or textured and then denoises them by deploying different denoising strategies for efficient denoising. The denoising algorithm is integrated into the SR approach, which uses a gradient profile-based constraint in a sparse representation-based framework to improve the resolution of MR images with reduced smearing of image details. This constraint regularizes the estimation of HR images such that the estimated HR image has gradient profiles similar to the gradient profiles of the original HR image. For this, the gradient profile sharpness (GPS) values of an unknown HR image are estimated using an approximated piece-wise linear relation among GPS values of LR and upsampled LR images. The experiments are performed on three different publicly available datasets. The proposed SR approach outperforms the existing unsupervised SR approach addressed for real MR images that exploits low rank and total variation (LRTV) regularization, by an average peak signal to noise ratio (PSNR) of 0.73 dB and 0.38 dB for upsampling factors 2 and 3, respectively. For the super resolution of noisy real MR images (degraded with 2% noise), the proposed approach outperforms the LRTV approach by an average PSNR of 0.54 dB and 0.46 dB for upsampling factors 2 and 3, respectively. The qualitative analysis is shown for real MR images from healthy subjects and subjects with Alzheimer’s disease and structural deformity, i.e., cavernoma.

Deep learning-enabled resolution-enhancement in mini- and regular microscopy for biomedical imaging

Artificial intelligence algorithms that aid mini-microscope imaging are attractive for numerous applications. In this paper, we optimize artificial intelligence techniques to provide clear, and natural biomedical imaging. We demonstrate that a deep learning-enabled super-resolution method can significantly enhance the spatial resolution of mini-microscopy and regular-microscopy. This data-driven approach trains a generative adversarial network to transform low-resolution images into super-resolved ones. Mini-microscopic images and regular-microscopic images acquired with different optical microscopes under various magnifications are collected as our experimental benchmark datasets. The only input to this generative-adversarial-network-based method are images from the datasets down-sampled by the Bicubic interpolation. We use independent test sets to evaluate this deep learning approach with other deep learning-based algorithms through qualitative and quantitative comparisons. To clearly present the improvements achieved by this generative-adversarial-network-based method, we zoom into the local features to explore and highlight the qualitative differences. We also employ the peak signal-to-noise ratio and the structural similarity, to quantitatively compare alternative super-resolution methods. The quantitative results illustrate that super-resolution images obtained from our approach with interpolation parameter α = 0.25 more closely match those of the original high-resolution images than to those obtained by any of the alternative state-of-the-art method. These results are significant for fields that use microscopy tools, such as biomedical imaging of engineered living systems. We also utilize this generative adversarial network-based algorithm to optimize the resolution of biomedical specimen images and then generate three-dimensional reconstruction, so as to enhance the ability of three-dimensional imaging throughout the entire volumes for spatial-temporal analyses of specimen structures.