In two experiments using the shuttlebox apparatus and negative (shock) reinforcement, goldfish learned to discriminate between same color signals differing only in brightness and to choose the brightness level paired with shock-omission to avoid shock. Experiment 1 compared two signal presentation procedures, one presenting only one signal at a time but allowing for successive alternation of signals within a trial (SUC) and the other presenting both signals simultaneously (SIM). SIM performance was superior to SUC in acquisition and showed more positive transfer with signal color changed (CC), more negative transfer with brightness-level—shock pairing reversed (R), and, although attenuated, more negative transfer with both signal color changed and shock pairing reversed (CC + R). Further, SIM relearning following reversal was faster than SUC. In Experiment 2, using the SIM procedure, eight transfer groups were tested for transposition with one of their original acquisition signals replaced in transfer by a signal bearing either the same or the opposite brightness relationship to the retained signal. Four transfer groups showed positive and four showed negative transfer effects, but all eight groups showed transfer performances consistent with a transpositional expectation. The present two experiments alone do not elucidate the mechanisms accounting for either the SIM superiority over SUC in Experiment 1 or the transposition effect in Experiment 2. These experiments show only that the transposition phenomenon can be supported by negative reinforcement procedures, which has not been demonstrated before. Now that techniques exist for producing negatively reinforced transposition, work investigating the underlying mechanisms and comparing them with the mechanisms of positively reinforced transposition can begin.