Wall Organs Research Articles

Histological and morphometrical evaluation of the urethral wall after bioresorbable stent implantation in male New Zealand White Rabbits: A preliminary study.

The aim of the study was the histological and morphometrical evaluation of the urethral wall at three time points after bioresorbable stent implantation in male New Zealand White Rabbits. The research was performed on 26 male New Zealand White rabbits aged 3-4 months and weighing 2.1-3.0 kg. Two models of bioresorbable sodium alginate-based stents were developed and implanted into the urethral lumen for one (T1), three (T3), and six weeks (T6). Sections of 5 µm thickness were cut from the urethra at intervals of 2 mm. The sliced sections were stained with hematoxylin-eosin (H&E), Van Gieson's (VG), Von Kossa, and Movat-Russell modified pentachrome (MOVAT) staining methods. The study provided valuable information for future models of urethral stents. The first model of the stent failed to fit the requirements due to inadequate mechanical properties. It curled up on itself losing the ability to adhere to the animals' urethra and was bioresorbed three weeks after implantation. The more rigid no. 2 stent was effective in widening the urethral lumen but did not biodegrade during the experiment. A comprehensive assessment of the second model's properties of biosorption and biointegration requires an extended observation of at least 12 months for an in depth morphological analysis. Stent migration is not likely to be caused solely by the mechanical properties of the urethra or urinary flow but mainly by muscle contraction of the organ wall.

Morphology of the Digestive Tube of the Amazonian Freshwater Stingray Potamotrygon wallacei (Elasmobranchii: Potamotrygonidae): A Stereological Approach.

This work aimed to describe and quantify the tissue components of the digestive tube of the neotropical freshwater stingray, Potamotrygon wallacei. For this, conventional histology and stereological methods were used to estimate tissue volume. The volumes of the four fundamental layers and the tissue components in the stomach (cardiac and pyloric) and spiral intestine were also estimated. In the cardiac stomach, the mucosa layer occupies 44.7% of the total volume of the organ wall. The gastric glands are the main components, and these structures alone represent 49.7% of this layer. This large number of gastric glands suggests a high potential for processing food items with a high protein content. The stereological methods were sensitive enough to show a reduction in the volume of the gastric glands from the cardiac region toward the pyloric region. Gastric glands are absent in the pyloric region of the stomach. However, the muscularis becomes thicker towards the pyloric region. The increase in smooth muscle thickness is due to the thickening of the inner muscular layer. This suggests that the role of the pyloric stomach may be related to the mixing of the chyme and assisting its passage to the spiral intestine. In the spiral intestine, data on the volume of the mucosa layer (and epithelial lining) suggest that the spiral valve has a large absorptive area. In several respects, the morphology of the digestive tube of P. wallacei is similar to that of other batoids. However, its slight morphological variations may be related to the habitat specificity of this species.

Free radical fragmentation and oxidation in the polar part of lysophospholipids: Results of the study of blood serum of healthy donors and patients with acute surgical pathology

The interaction of reactive oxygen species with cell membrane lipids is usually considered in the context of lipid peroxidation in the nonpolar component of the membrane. In this work, for the first time, data were obtained indicating that damage to human cell membranes can occur in the polar part of lysophospholipids at the interface with the aqueous environment due to free radical fragmentation (FRF) processes. FRF products, namely 1-hexadecanoyloxyacetone (PAc) and 1-octadecanoyloxyacetone (SAc), were identified in human serum, and a GC-MS method was developed to quantify PAc and SAc.The content of FRF products in serum samples of 52 healthy donors was found to be in the range of 1.98–4.75 μmol/L. A linear regression equation, CPAc&SAc (μmol/L) = 0.51 + 0.064 × years, was derived to describe the relationship between age and content of FRF products. In 70 patients with acute surgical pathology in comparison with the control group of healthy donors, two distinct clusters with different concentration levels of FRF products were revealed. The first cluster: groups of 43 patients with various localized inflammatory-destructive lesions of hollow organ walls and bacterial translocation (septic inflammation) of abdominal cavity organs. These patients showed a 1.5–1.9-fold (p = 0.012) decrease in the total concentration of PAc and SAc in serum. In the second cluster: groups of 27 patients with ischemia-reperfusion tissue damage (aseptic inflammation), – a statistically significant increase in the concentration of FRF products was observed: in 2.2–4.0 times (p = 0.0001).The obtained data allow us to further understand the role of free-radical processes in the damage of lipid molecules. FRF products can potentially be used as markers of the degree of free-radical damage of hydroxyl containing phospholipids.

ГИПЕРКАЛЬЦИЕМИЯ В ПРАКТИКЕ ИНТЕРНИСТА

The relevance of the study of hypercalcemia is determined by its prevalence among the population in different age groups which varies from 0.17 to 3.9 cases per 100 people. Most often, hypercalcemia is asymptomatic, but at the same time, this process of calcification of the walls of blood vessels and internal organs as well as bone resorption lead to the development of arterial hypertension, atherosclerosis, osteoporosis, which increase the risk of poor prognosis, especially in the group of elderly patients. Considering that hypercalcemia is believed to be a life-threating condition, medical specialists are challenged to timely diagnose this condition in order to prescribe adequate treatment for the underlying disease and avoid clinical manifestations of both chronic and acute hypercalcemia.

FEATURES OF THE METABOLISM OF SIALOGLYCOPROTEINS IN THE TISSUES OF THE LIVER, STOMACH AND SMALL INTESTINE OF RATS UNDER IMMOBILIZATION STRESS

Introduction. Immobilization stress leads to the intensification of free-radical reactions leading to the destruction of sialoglycoproteins of the gastroduodenal zone. The aim of the study. To evaluate the features of the metabolism of sialoglycoproteins in the tissues of the liver, stomach and small intestine of rats under immobilization stress. Methods. Total sialic acids (TSA) and levels of sialidase activity (SA) were determined in the serum, liver, stomach, and small intestine tissues of rats by standard testing systems in dynamics on the 21st, 35th, 60th and 90th days of observation. Results. The blood serum of laboratory rats showed a maximum increase in total sialic acids and sialidases by 35 days of stress exposure, while in the liver, on the contrary, a decrease in the above-mentioned indicators. In the mucous overlays of the stomach and small intestine, the number of TSA increased throughout the dynamics of observation, and the activity of sialidase decreased by day 21 and increased in the following days of the experiment. In the wall of the stomach and small intestine of experimental rodents, the level of TSA increased by the 35th day of observation. Conclusion. During immobilization stress in the exchange of sialoglycoproteins, a compensatory predominance of anabolic processes in liver tissues was noted against the background of the prevalence of catabolism in the mucosal layer of the stomach and small intestine on days 21, 35 of observation, changes in the walls of the above organs are less pronounced and manifest themselves at a later date.

Botyroid Rhabdomyosarcoma of the Vagina in A Two-month-old Girl

Botyroid rhabdomyosarcoma is the most common malignant mesencymal tumor of the vagina in children and infants. It is a malignant tumor that arises from immature skeletal muscle cells (i.e., rhabdomyoblasts) lining the walls of hollow organs, including the vagina, bladder, and biliary tract. This cancer most commonly affects children under the age of 4, although it can sometimes present in older individuals. Botyroid sarcoma is usually found in the vagina during infancy and early childhood, in the cervix during reproductive years, and in the corpus uteri during postmenapausal years. Treatment is combination of surgery with adjuvants chemotherapy and radiotherapy. We report a case of two-month-old girl presented with a history of vaginal bleeding and a mass protruding from vagina. Diffusion Magnetic Resonance Imaging (MRI) showed an approximately 6 cm mass in rectovaginal septum. Excisional biopsy from the vagina confirmed the diagnosis of botyroid rhabdomyosarcoma. After biopsy the patient received multiple cycles of chemotherapy. The patient is disease-free on remission at this time.

Comparative Evaluation of the Results of Radical Surgical Treatment of Patients with Muscle-Invasive Bladder Cancer

Malignant neoplasms of the bladder with invasion into the muscle layer of the organ wall (MIBC) are recognized as a highly aggressive pathology with a poorly predicted outcome, a high mortality rate, involving patients of any age without gender differences. Radical surgery’s in patients with MIBC has long and highly established itself as one of the main methods of treatment. However, the results of open surgical interventions are still accompanied by a high level of complications. The materials of medical literature (PubMed, CrossRef) for 2000–2023 were studied, with the issues of comparative evaluation of the results of surgical treatment of patients with MIBC, complications of the postoperative period. The search was conducted on the key phrases “bladder cancer”, “open cystectomy”, “robot-assisted cystectomy”, “cancer-specific survival”. The widespread introduction of video-endoscopic methods of treating patients with this disease into clinical practice has made it possible to speak about a decrease in the volume of intraoperative blood loss, the frequency of infectious complications of the wound and inpatient stay. The development of robot-assisted bladder surgery (RARC) demonstrates a number of advantages of using the daVinci robot in terms of reducing the number of postoperative complications during the traditional to study the 30–60–90-day follow-up periods. The issues of rehabilitation of patients after various methods of urine diversion, the medical-economic aspects for RARC in expert urological centers and the feasibility of the widespread introduction of this technique in medical institutions remain unclear. The coverage of these issues in the medical scientific literature is ambiguous, and therefore requires additional analysis.

Tissue structure transformations of the gallbladder in chronic calculous cholecystitis

Aim. To determine the morphofunctional characteristics of tissue changes in the structure of the gallbladder in chronic calculous cholecystitis.Materials and Methods. The macro- and microscopic structural walls of the gallbladder were studied using the method of injection anatomical dissection, histology, and morphometry in 61 patients (aged 36 to 60) with chronic calculous cholecystitis.Results and Discussion. The results of a morphological study show that in chronic calculous cholecystitis, changes are always observed in all layers of the gallbladder wall. The most severe dystrophic and sclerotic changes are observed in the mucous membrane and submucosal base, since, this layer has in functional, topographical and quantitative features, as well as a direct contact with stones (mechanical impact).Conclusion. Transformation of the gallbladder in chronic calculous cholecystitis are characterized by vascular tissue restructuring of the structures of the organ wall, accompanied by the phenomena of adaptive, exudative and proliferative transformations, and in the later stages (5-6 years) undergo sclerotic changes in the gallbladder wall.

Proventricular and ventricular gastrointestinal stromal tumor (GIST) in a scarlet macaw (Ara chloropterus)

GISTs can be benign or malignant, and they have been reported in a wide variety of species, including humans, non-human primates, dogs, and cats; however, reports of this tumor in birds are rare. These neoplasms are seen in the gastrointestinal tract and are derived from the Cajal Cells responsible for regulating gastrointestinal motility. We report a case of a gastrointestinal stromal tumor in a scarlet macaw (Ara chloropterus) that was referred to necropsy at the Veterinary Hospital of the Federal University of Paraíba (UFPB), Northeastern Brazil. Macroscopically, the proventriculus and ventriculus were dilated, and the walls of both organs were thickened by a firm, ulcerated mass covered by fibrin strands. In the histopathology, the proventriculus presented an expansive, non-encapsulated neoplasm formed by homogeneous spindle cells, with eosinophilic cytoplasm, indistinct borders, mild to moderate pleomorphism, and rare mitotic figures, being suggestive of a leiomyoma. In immunohistochemistry, a positive multifocal immunolabeling for the C-KIT proto-oncogene was observed in the cytoplasm of neoplastic spindle cells, thus compatible with a gastrointestinal stromal tumor (GIST). The occurrence of GISTs in avian species might have been overlooked, and the authors recommend that previously reported gastrointestinal leiomyomas or leiomyosarcomas be tested for this immunomarker to rule out the possibility of GISTs.

EVOLUTION OF MORPHOLOGICAL CHANGES OF THE GALLBLADDER WALL AT DIFFERENT TERMS OF PERFORMANCE OF LAPAROSCOPIC CHOLECYSTECTOMY

Gallstones are present in approximately 10-15 % of the adult population in Western countries, with 1-4 % of patients experiencing symptoms each year. Recent guidelines have confi rmed that laparoscopic cholecystectomy is the mainstay of treatment for acute calculous cholecystitis, but the optimal timing of cholecystectomy is still a matter of debate and research. The purpose of the study is to improve the results of surgical treatment of patients with acute obturation calculous cholecystitis by clinical and morphological substantiation of the optimal terms of laparoscopic cholecystectomy.Material and methods. To increase the safety of laparoscopic cholecystectomy, a histological study of micropreparations of the gallbladder wall after laparoscopic cholecystectomy was conducted in 30 patients, who were divided into 2 groups: 1 group – features of morphological and structural changes in the gallbladder wall up to 7 days after the onset of an attack of biliary colic (18 patients; 60 %) and group 2 – peculiarities of the morpho- structural changes of the gallbladder after 7 days from the onset of an acute attack of biliary colic (12 patients, 40 %);The results. On the basis of microscopic examination of histological preparations, it can be stated that in the early stages of the fi rst attack of cholecystitis, acute infl ammation of various morphological types (non-destructive and destructive) occurs in the wall of the gallbladder. In the late period (after 7 days), sclerotic changes develop both in the organ wall and perivesical. The main role in the pathogenesis of the remodeling of the structural components of the gallbladder is played by deep hemodynamic disorders and the prolongation of the infl ammatory process. Circulatory hypoxia, increasing under such conditions, activates the sclerosing process.

Radiomics-based T-staging of hollow organ cancers.

Cancer growing in hollow organs has become a serious threat to human health. The accurate T-staging of hollow organ cancers is a major concern in the clinic. With the rapid development of medical imaging technologies, radiomics has become a reliable tool of T-staging. Due to similar growth characteristics of hollow organ cancers, radiomics studies of these cancers can be used as a common reference. In radiomics, feature-based and deep learning-based methods are two critical research focuses. Therefore, we review feature-based and deep learning-based T-staging methods in this paper. In conclusion, existing radiomics studies may underestimate the hollow organ wall during segmentation and the depth of invasion in staging. It is expected that this survey could provide promising directions for following research in this realm.

Features of diagnosis and treatment of false post-traumatic diaphragmatic hernias

Damage to the diaphragm domes followed by the formation of false diaphragmatic hernias (FDH) occurs with closed and open trauma to the chest and/or abdomen. In this case, the mobile organs of the abdominal cavity, being uncovered by the parietal peritoneum, move to the pleural cavity and, with a small defect in the diaphragm, can be infringed up to necrosis of the organ wall. The rare occurrence of such pathology, the paucity of clinical symptoms, explains the high error rate in diagnosis, especially in primary health care. We have experience in two clinics over 34 years in the treatment of 12 patients with FDH. The left dome was damaged in 10 patients, the right dome in two. The age of damage to the diaphragm ranged from several hours to 7 years. Five clinical examples are presented, combined with the late diagnosis of the disease, the difficulties of diagnosis, even in a clinic using modern imaging tools. All patients are operated on both as planned and as an emergency. Endovideosurgical technologies for diagnosis and treatment are used only in 2 patients. 2 (16.67 %) patients died with a false diaphragmatic hernia due to a severe closed injury against the background of traumatic and hemorrhagic shocks. Conclusions. A clear explanation of the origin of scars on the skin of the chest and abdomen, clarification of the mechanisms and severity of closed injuries in the https://panor.ru/articles/osobennosti-diagnostiki-i-lecheniya-lozhnykh-posttravmaticheskikh-diafragmalnykh-gryzh/92437.html

The Efficacy and Results of Medical Treatment in Postoperative Ileus

ABSTRACT Background: Postoperative paralytic ileus refers to the disruption of the normal coordinated propulsive motor activity of the gastrointestinal system following surgery. Surgery causes inflammation in the muscle walls of organs with an intestinal lumen that, in turn, leads to a decrease in intestinal motility. Aim: The aim of this study was to investigate the efficacy of gastrografin, neostigmine, and their combined administration in patients diagnosed with paralytic ileus in the postoperative period. Patients and Methods: One-hundred twelve patients were included from January 2017 and November 2019. The retrospective study is involving prolonged postoperative ileus cases following colorectal surgery. The effect of gastrografin, neostigmine, and gastrografin neostigmine combination was compared retrospectively in the treatment of prolonged ileus after surgery. Results: The study covered 112 patients. Gastrografin was administered to 63 patients; neostigmine was administered to 29, while 20 patients received the combination of the two. Data pertaining to the comparison of the two groups revealed that patients in the gastrografin group were discharged earlier than those in the neostigmine group. Further, patients in the combined group had earlier gas and/or stool discharge and were also discharged from the hospital earlier than those in the neostigmine group. Conclusion: Gastrografin and combined use of gastrografin and neostigmine are effective and viable methods for postoperative ileus cases. Gastrografin can safely be used in patients with anastomoses.

Wnt Signaling in the Gastrointestinal Tract in Health and Disease

Wnt signaling involves multiple pathways that contribute to organ development, cell fate, inflammation, and normal stem cell renewal and maintenance. Although the homeostasis of stem cells in the gastrointestinal (GI) tract highly depends on the Wnt signaling pathway, this regulation is impaired in cancers and in aging. Overactive (uncontrolled) Wnt signaling can induce GI epithelial cancers such as colon and gastric cancer. Overactive Wnt signaling can also contribute to the initiation and progression of gastrointestinal stromal tumor, which is the most common human sarcoma occurring in the walls of the digestive organs, mainly the stomach and small intestine. Wnt expression is positively associated not only with the progression of oncogenesis but also with resistance to chemotherapy and radiotherapy. Of note, recent reports show that decreased Wnt signaling is related to intestinal stem cell aging and that overactivated Wnt signaling leads to gastric pacemaker stem cell aging in tunica muscularis. These findings indicate that Wnt signaling has different crucial aspects of cell fate determination with age in GI tunica mucosa and muscularis. In this review, we summarize the most recent advances in our understanding of Wnt signaling pathways and their role in regulating key aspects during development, carcinogenesis, inflammation, and aging, with the ultimate goal of identifying novel therapies.

Morphometric indicators of the wall of the crop of broiler chickens when using some biologically active feed additives

Purpose: describe the histological structure and morphometric parameters of the crop of broiler chickens and determine the effect of the multienzyme preparation and humic substances on its structure.Materials and methods. The multienzyme preparation contained beta-gluconase, pentosanase, proteolytic, lipolytic, cellulolytic and amylolytic enzymes. Group 1 received the main diet, balanced in all respects. Group 2 received an enzyme preparation 1 g/kg of feed in addition to the main ration. Group 3 received 1 g/kg of sodium humate feed in addition to the main diet. Group 4 received both an enzyme preparation and sodium humate, 1 g/kg of compound feed, in addition to the main diet. Withdrawal from the experiment occurred at 28 and 49 days of age. For research, 5 samples of crop from the group were selected.Results. Studies have shown ongoing processes of growth and differentiation of structures within 49 days of cultivation. The thickness of the crop wall in birds of the control group decreases by 11.8 %, which may be due to an increase in the size of the organ, its fullness and the mechanical effect of the feed components on its wall. The decrease in the thickness of the wall of the crop by 49 days of age occurs mainly due to a decrease in the thickness of the muscular membrane by 38.5 %. The relative mucosal height increases with bird age from 44 % at 4 weeks of age to 53 % at 49 days of age. An increase in the height of the epithelium by 6.4 % is observed, and the thickness of the lamina propria decreases with age. By 49 days of age, in all experimental groups, the muscular coat is significantly larger, and the submucosa is smaller than in the control group. The maximum thickness of the muscular membrane in group 2 is 1.6 times more than in group 1. The relative height of the epithelium is minimal in groups 2 and 3 - 44 %. In these groups, a significantly greater wall thickness was observed than in the control group, mainly due to the muscular layer. Sodium humate does not adversely affect the structure of the crop. Long-term use of the multi-enzyme complex irritates the mucous membrane in the first period of cultivation. This is expressed in an increase in the thickness of the epithelium by 20%. Further, the wall adapts to the irritating effect of the drug, and in the second period of cultivation, no negative effect on the crop mucosa was found in this group. The complex use of additives in the first period of cultivation leads to inhibition of the development of all structures of the mucosa, except for the epithelium. Apparently, the negative effect of the enzyme preparation is affecting. But at the same time, the irritating effect of additives on the mucous membrane is not observed, since the height of the epithelium does not change significantly. Apparently, in this case, we see a positive adaptogenic effect of humic substances on the organ wall. In the second growing period, no negative effect of additives on the crop wall was found.Conclusion. The wall thickness of the crop decreases with age, due to a decrease in the thickness of the muscular membrane. The height of the epithelium in the mucosa increases. Long-term use of sodium humate does not adversely affect the histological structure of the crop. The continuous use of enzyme preparations irritates the mucous membrane of the crop in the first period of cultivation, in the second period of such an effect of the additive is not observed. The combined use of these additives does not irritate the wall of the crop.

Changes in the content of fucoglycoproteins in the mucous membranes and walls of the stomach and small intestine of alcoholized rats under immobilization stress

The article highlights the features of the metabolism of fucoglycoproteins in liver homogenates, the wall and mucous membrane of the stomach and small intestine in experimental models: alcoholic liver disease and prolonged alcoholization of rats under immobilization stress. Fucoglycoprotein metabolism was assessed for 90 days by the level of total fucose and fucosidase activity. To evaluate the implementation of the experiments, a biochemical analysis of markers of liver pathology was carried out. In the dynamics of experiments, there was an increase in transaminases and dyslipidemia in the blood serum of rats, as well as an increase in the level of corticosterone, especially in rats against the background of immobilization stress. In the exchange of fucose-containing glycoproteins in the blood serum, in the tissues of the liver, stomach and small intestine of experimental animals, multidirectional metabolic changes were monitored, namely, an increase in catabolism processes in the first days of observations in alcoholized rodents, which may be associated with the cytolytic effect of ethanol in the studied tissues. In these organs, the total amount of fucose is higher in the mucosa layer than in the organ wall. At the same time, in a series of experiments with immobilization stress against the background of ethanol administration, activation of anabolism of oligosaccharide fragments for the synthesis of fucoglycoproteins was noted in the second half of the experiment, associated with compensatory adaptive mechanisms.

Retraction of the article “The problems of classification and specific features of morphogenesis of glands located in the walls of hollow internal organs”

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Tumor traction techniques in intraluminal endoscopic surgery of the digestive tract

Endoscopic submucosal dissection (ESD) is currently an advanced method of treating early cancer of the upper and lower gastrointestinal tract. The ESD allows to remove tumors of any size in an en-bloc, unlike endoscopic mucosal resection (EMR), when we can remove lesions completely only less than 20 mm in size. Despite this advantage, ESD is accompanied by more perforations and bleeding than EMR. Difficult anatomical localization, an unexpressed muscle layer of the organ wall, fibrosis of the submucosal layer and insufficient experience of the operator are the main causes of incomplete resections of the tumor, the development of complications and relapses. The described methods of tumor traction during submucosal dissection allow increasing the value of en-bloc resection and achieving histologically complete resection. We have presented a wide variety of different methods of tumor traction when performing ESD. We have noted both advantages and disadvantages of traction techniques in endoscopic intraluminal surgery.

Фенотипическая трансформация гладких миоцитов стенки желчного пузыря при развитии некалькулезного холецистита

The aim of the study was to analyse ultrastructural changes in the smooth muscle tissue of different gallbladder sections during the development of experimental acalculous cholecystitis. Materials and methods. The research was performed on 20 guinea pigs. The duration of the experiment ranged from 4 to 15 days. Each experimental and control group included 5 animals. A standard model of chronic acalculous cholecystitis was used, in which ligation of the proximal part of the common bile duct is accompanied by inflammation and impaired gallbladder motility. Results. In guinea pigs with cholecystitis, on day 15 of the experiment using electron microscopic examination we revealed cells in the smooth muscle tissue that retain a well-structured contractile apparatus, represented by separate thin bundles of myofilaments, and a developed synthetic apparatus. The ultrastructural organization of this type of cells is quite characteristic of myofibroblasts. Studying the modulation of gallbladder smooth muscle cells in an experiment, we need to take into account that in the conditions of inflammation and impaired gallbladder motility, during the restructuring of the intercellular matrix, myofibroblasts can appear, causing the development of sclerotic processes in the organ wall due to their ability to express high levels of collagen and glycosaminoglycans, as well as numerous other extracellular matrix molecules and fibrogenic cytokines. The research showed that adenomyomatous hyperplasia of the gallbladder wall is accompanied by proliferation of myofibroblasts and smooth muscle cells. Thus, impaired stromal-epithelial interactions can be assumed to underlie the pathology in adenomyomatous hyperplasia of the gallbladder.

Comparison of different staining methods for the nematode Strongyloides papillosus isolated from rabbits

Strongyloidiasis in rabbits is a disease caused by Strongyloides papillosus helminths, manifested by skin inflammation, diarrhea, weight loss, and stunting of animals in growth and development. Confirmation of invasion is carried out mainly by required methods of helminthoovoscopy and helmintholarvoscopy. The most well-known methods of helmintholarvoscopic diagnosis of feces for strongyloidiasis are the method of D. Baermann (1917) and its modifications according to V. I. Shilnikov (1983), the method using the “asterisk” device (according to V. F. Nikitin and T. Pavlasek, 1988), the method of coprohelmintho-larvoscopic rings (according to S. I. Ponomar, N. M. Soroka, 2007), the method of T. I. Popova, standardized by S. I. Ponomar (2003). There are no laboratory methods with 100 % reliability at the present stage. Therefore, our studies on improving the lifelong helminthocoprological diagnosis of strongyloidiasis in rabbits are relevant. Our work aimed to compare different methods of staining the pathogen S. papillosus. Laboratory studies were carried out in the laboratories of the Department of Parasitology and Veterinary Sanitary Expertise of the Dnipro State Agrarian and Economic University. For staining S. papillosus, 20 dyes were used: 1 % alizarin red, 1 % brilliant blue, Turk's solution, 1 % brilliant green, 1 % methylene blue, 1 % amido black 10B, 1 % eosin, Ziehl's solution, Lugol's solution, 1 % сarbolic gentian violet, Romanowsky-type, 1 % Sudan, 1 % bromophenol blue, 1 % orange G, 1 % bromocresol green, 1 % safranin, Zadorozhny-Dozmorov's-type, Mikhin's-type, Muromtsev's-type. We have proposed 20 dyes for staining the internal organs of larvae and free-living males and females pathogens of Strongyloides without or with temperature fixation. Live pathogens S. papillosus, without temperature fixation, pass only Lugol's and safranin's solutions. All other dyes showed the highest staining, but only when the preparation was heated for 2–3 seconds to 60 °C. However, the dyes showed different degrees of coloration. Bromocresol green stained the walls of the internal organs of the helminth. Namely, the nematodes acquired a green color, and the membranes of a part of the esophagus and intestines became dark green. During microscopy, this allowed accurate identification and differentiation into larvae and free-living males and females of S. papillosus and simplified their counting.