Determination Of Organochlorine Pesticides Research Articles

Vortex-assisted dispersive micro-solid-phase extraction using silica-supported Fe2O3-modified khat (Catha edulis) biochar nanocomposite followed by GC-MS for the determination of organochlorine pesticides in juice samples

In this paper, dispersive micro-solid phase extraction technique was developed for the purpose of extracting and preconcentrating organochlorine pesticide residues in juice samples before their separation and quantitative analysis by gas chromatography-mass spectrometry. A sorbent composed of a silica-supported Fe2O3-modified khat leftover biochar nanocomposite (SiO2-Fe2O3-KLBNC) was implemented in the process. To improve the dispersion of the sorbent in the solution, vortex mixer was employed. Experimental parameters influencing the performance of the method were optimized, and the optimal conditions were established. With these conditions, linear dynamic ranges ranged from 0.003 to 100.0 ng/mL were achieved, with a correlation coefficient (r2) ≥ 0.9981. The limits of detection and quantification, determined by signal-to-noise ratios of 3 and 10, respectively, were found to be in the ranges of 0.001–0.006 ng/mL and 0.003–0.020 ng/mL. Intra- and inter-day precision, values ranging from 0.3–4.8% and 1.7–5.2% were obtained, respectively. The matrix-matched extraction recoveries demonstrated favorable outcomes, falling within the range of 83.4–108.3%. The utilization of khat leftover as an adsorbent in contemporary sample preparation methodologies offers a cost-effective alternative to the currently available, yet expensive, adsorbents. This renders it economically viable, particularly in resource-constrained regions, and is anticipated to witness widespread adoption in the coming future.

Gas Chromatographic Determination of Residual Concentrations of a Mixture of Organochlorine Pesticides in Food Products

Purpose of research. The aim of the work is to optimize the conditions for gas chromatographic determination of a mixture of eight organochlorine pesticides: aldrin, hexachlorobenzene, heptachlor, α-hexachlorocyclohexane, γ-hexachlorocyclohexane, dichlorodiphenyltrichloromethylmethane, dichlorodiphenyldichloroethane and dichlorodiphenyldichloroethylene.Methods. For chromatographic studies of organochlorine pesticides we used a Shimadzu GC-2010Plus gas chromatograph (Shimadzu, Japan) equipped with an automatic equilibrium vapor sample dispenser NT200N, electron-capture detector based on 63Ni and quartz capillary nonpolar column "Chromatek" SPB-5 (30 m, 0.32 mm, film thickness on the basis of phenyl(5%) and dimethyl(95%) polysiloxanes 0.5 μm) with an operating temperature range of 60°C 320/340°C. The chromatograms were processed in the application LabSolutions GPC.Results. On the basis of experimentally obtained data the optimal conditions for gas chromatographic determination and separation of eight-component mixture of organochlorine pesticides were selected, namely: the value of mobile phase flow division 1:10, carrier gas flow rate – 1.0 ml/min, two-stage temperature programmable column mode from 160 to 240°C with the rate of increase of 5°C/min. The metrological characteristics of the analysis and validation of the results of the proposed technique with the method of high-performance liquid chromatography were evaluated. Good reproducibility of chromatography results with the possibility of detection of organochlorine pesticides at the level of 10-6 – 10-5 μg/cm3 was proved. Application of Fisher's test has shown absence of significant differences in reproducibility of sampled populations the investigated methods of chromatographic analysis of COPCs are equally accurate. Extraction sample preparation of samples of real objects was proposed, the calculated extraction degrees >84% allow to recommend the technique for quantitative determination of analytes.Conclusion. The method of gas chromatographic analysis was tested for determination of organochlorine pesticides in samples of drinking water and baby puree (Russia) by additive method. Exceeding the norms of residual contents of organochlorine pesticides in the samples of the food products under study was not detected.

Dispersive solid‐phase extraction for the determination of organochlorine pesticides in fruit juice samples using iron‐doped zinc oxide nanoparticles supported with silica as a sorbent

AbstractAn efficient analytical method has been developed based on dispersive solid‐phase extraction followed by gas chromatography‐mass spectrometry for the determinations of 13 organochlorine pesticides in fruit juice samples. In this method, dispersive solid phase extraction was used for the extraction of target analye using iron‐doped zinc oxide nanoparticles supported with silica as a sorbent. Different experimental parameters affecting the extraction efficiencies the proposed method were carefully optimized. Under the optimum conditions, the calibration graphs were linear in the concentration range of 0.6–100 ng/mL with coefficients of determinations in the range of 0.9927–0.9991. The limits of detection and quantification determined as 3 and 10 times the signal‐to‐noise ratio were in the range of 0.01–0.03 and 0.6–1.0 ng/mL, respectively. Intra‐ and inter‐day precision studies of the proposed method, expressed as relative standard deviations, were in the range of 2.1%–9.2% and 2.2%–9.7%, respectively. The recoveries of the spiked fruit juices samples were in the range of 81.4%–105.1% with the corresponding relative standard deviations ranging from 1.0%–8.8%. In general, the proposed method demonstrated satisfactory analytical performance. Thus,it could be used as simple and attracive alternative method for the extraction of organochlorine pesticides from fruit juice sample and other related matrices.

Establishment of a GC-HRMS-IDMS-based modified QuEChERS approach for rapid, reliable, and simultaneous determination of organochlorine pesticides in soil

Organochlorine pesticides (OCPs), a class of organic pollutants, are highly toxic and persistent in soil and can have harmful effects on humans, organisms, and agricultural environments when bioaccumulated through the food chain. The existing methods for OCP analysis are complex and time consuming; thus, developing a rapid and reliable method for quantifying and monitoring soil-bound OCPs is essential. This study demonstrates the feasibility of replacing the existing official method for OCP analysis with a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method based on GC-HRMS-IDMS. In this modified QuEChERS method, acetonitrile was replaced with ethyl acetate as the extraction solvent. We also assessed the optimal extraction salt (Original, AOAC, and EN) and dispersive solid-phase extraction clean-up sorbent. The limits of detection (LODs) and quantification (LOQs) of the modified QuEChERS method for 24 OCPs ranged from 0.02 to 0.24 ng/g and from 0.06 to 0.3 ng/g, respectively. The recovery rates at the LOQ level were 59.6–115.9 % for the Original method, 60.5–112.5 % for the AOAC method, and 59.6–115.9 % for the EN method. Furthermore, soil samples contaminated with endosulfan were analyzed using the three QuEChERS methods to compare their contents and deviation. EN exhibited more accurate results than Original and AOAC. Thus, the sample processing method using QuECHERS can replace the existing method.

Dispersive solid phase extraction using zinc oxide graphitic carbon nitride as sorbent followed by dispersive liquid–liquid microextraction for the determination of organochlorine pesticides from fruit juice samples

ABSTRACT In the present work, we proposed a novel method; dispersive solid-phase extraction followed by dispersive liquid–liquid microextraction for selective extraction and preconcentration of organochlorine pesticides in fruit juice samples. Parameters affecting the performances of both extraction steps have been rigorously studied and optimized. For the dispersive solid-phase extraction, zinc oxide-graphite carbon nitride was used as a sorbent for the first time. In the extraction, 100 mg of the sorbent was added to an aqueous solution. The content was then vortexed thoroughly to disperse the sorbent into the sample solution and to enhance the transfer of the analytes to the extraction phase (sorbent). The extracted analytes were then desorbed using 500 μL methanol. Subsequently, for the dispersive liquid-liquid microextraction, 50 μL of chloroform (as an extractant) was added to methanol-desorbed analytes and then rapidly injected into 5 mL deionised water. After centrifuging, 35 μL of the sedimented phase was withdrawn into an auto-sampler vial, and then 1 μL was injected into a gas chromatography-mass spectroscopy analysis. Under the optimum conditions, the proposed method showed satisfactory analytical performance characteristics: linearity ranging from 0.1–10.24 µg/L with coefficients of determinations (R2) from 0.9948–0.9995; The limit of detections ranging from 0.004–0.01 µg/L; intra- and inter-day precisions expressed as relative standard deviations ranging from 1.1–7.8%, and extraction recoveries varying from 80.8–109.2%. Generally, the proposed method is selective and efficient for the extraction and preconcentration of the target analytes from fruit juices and related matrices.

Determination of organochlorine pesticides from juice samples using magnetic biochar-based dispersive micro-solid phase extraction in combination with dispersive liquid-liquid microextraction

Analysis of pesticide residue levels in juice beverages is important to ensure safe consumption and avoid global trade concerns associated to pesticide contaminations. A simple, inexpensive and effective method was developed for the determination of organochlorine pesticides (OCPs) in bottled juice drinks using GC-MS. Sample pretreatment was performed using dispersive solid-phase microextraction (D-μ-SPE) for matrix desorption and dispersive liquid-liquid microextraction (DLLME) for analyte enrichment. In this study, an affordable and effective sorbent for the adsorption of OCPs from juice samples was synthesized from avocado seeds mixed with magnetic precursors for D-μSPE. The ground avocado seeds combined with a magnetic precursor nanocomposite were characterized using various instruments including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Brunauer-Emmett-Teller (BET) analysis. The solution obtained from D-μ-SPE desorption was used as a dispersant for the subsequent DLLME, which made the combination of D-μ-SPE with DLLME much easier. The effectiveness of the method was enhanced by optimizing the influential parameters in both D-μ-SPE and DLLME. Then after, the optimal values were determined for the real sample analysis. Accordingly, there was good linear dynamic range with a coefficient of determination (r2) ≥ 0.9989. The limit of detection and quantification were 0.02–0.69 and 0.06–2.10 ng/L respectively. The method showed high enrichment factors ranging from 96 to 313 with recoveries of 87–100 %. Intraday and interday precisions were ≤4 %. Compared with other reported methods, this method is a one-step, simple, cheap, fast, and environmentally friendly alternative and straightforward method for adsorbing organochlorine pesticides from sample solutions. These results demonstrates the high potential of the proposed method for the extraction and cleanup of contaminants in selected juices and other related samples.

New approaches to the determination of organochlorine pesticides in food products and food raw materials

Introduction. The current methods for determining the residual amounts of organochlorine pesticides – 4,4-dichlorodiphenyltrichloromethylmethane (DDT) and its metabolites, hexachlorocyclohexane (HCH) (α, β, γ isomers) in food products and food raw materials, subject to mandatory declaration for their content, despite a fairly high sensitivity, are characterized by low measurement selectivity and are based on the use of obsolete equipment, time-consuming sample preparation. 
 Material and methods. In the work, the method of gas chromatography with mass spectrometric detection on a gas chromatograph “Khromatek-Kristall 5000.2” was used. Sample preparation was carried out by the method of matrix solid-phase dispersion, based on the destruction and dispersion of the analysed sample together with florisil in order to obtain a free-flowing dry mixture.
 Results. The effectiveness of the method has been proven by validation on 16 model samples of food products of animal origin of various compositions. The versatility of the analysis procedure is ensured by the systematization of food products according to their structural and mechanical properties into groups: liquid, having a finely dispersed structure and a high water content (group A); viscous and pasty products having a fine structure (group B); products of animal origin requiring grinding and dispersion (semi-solid and solid), having a high water and/or fat content, not free-flowing (Group B); dry products of animal origin (dried or freeze-dried), and/or free-flowing powdered products with a low water content that require wetting, grinding and dispersion (Group D). Ingredient composition (water, fat, protein, hydrocarbons) does not significantly affect the stability and reproducibility of the developed method.
 Limitations. The method has been tested and validated on samples of food products of animal origin. 
 Conclusion. The developed method for the determination of DDT and its metabolites, HCH (isomers α, β, γ), providing a range of measured concentrations (0.01–1.0 mg/kg) will make it possible to control the content of substances in all groups of food products of animal origin identified in the TR CU 021/11 “On food safety”, including products for baby food.

Deep eutectic solvent-based dispersive liquid–liquid microextraction for determination of organochlorine pesticides in water and apple juice samples

In this work, we developed a deep eutectic solvent-based dispersive liquid–liquid microextraction (DES-based DLLME) method for the extraction and preconcentration of organochlorine pesticides (OCPs) from water and apple juice samples. Different DESs were synthesized by mixing menthol with carboxylic acids such as acetic, formic, and lactic acids. Among them, DES synthesized by mixing menthol with formic acid at a molar ratio of 1:1 showed the highest extraction efficiency for the target OCPs compared to other DESs. Various parameters affecting the method's extraction efficiency, including volume of DES, ionic strength, and centrifugation rate and time, were optimized. Under optimum conditions, matrix-matched calibration curves were separately constructed using water and apple juice samples. The obtained calibration curves have exhibited wide linearity ranges for both matrices. The limits of detections (LODs) and quantifications (LOQs) of the method ranged from 0.01 to 0.04, and 0.10–0.14 ng mL−1 for water and 0.05–0.09 and 0.22–0.41 ng mL−1 for apple juice samples, respectively. Similarly, the trueness of the method studied by spiking the samples at three concentration levels showed recovery values ranging from 80.1 to 112.5 % for both water and apple juice matrices. The findings demonstrated that the proposed DES-based DLLME method is rapid, simple, and environmentally benign. Thus, it could be used as an alternative method for determining OCPs in water, apple juice, and other related matrices.

Optimising the Operating Parameters of Oil Palm Frond Silica-based Dispersive Solid Phase Extraction for Determination of Organochlorine Pesticides

Optimising the Operating Parameters of Oil Palm Frond Silica-based Dispersive Solid Phase Extraction for Determination of Organochlorine Pesticides

Effervescent powder-assisted floating organic solvent-based dispersive liquid-liquid microextraction for determination of organochlorine pesticides in water by GC–MS

An effervescent powder-assisted floating organic solvent-based dispersive liquid-liquid microextraction was introduced for determination of 13 organochlorine pesticides in water samples. In this method, a less toxic low-density organic solvent was used as extraction solvent. The extraction solvent was dispersed in to the aqueous sample via CO2 bubbles, in-situ generated up on addition of water to a falcon tube containing the mixture of effervescent powder precursors as well as the extraction solvent. Various experimental parameters such as effervescent and its weight fractions, extraction solvent type and its volume, the total mass of effervescent precursors, and the effect of salt were investigated and the optimal conditions were established. Under the optimum conditions, the proposed method exhibited good linearity for all target pesticides with the coefficient of determinations varying from 0.9981 to 0.9997. The limits of detection and quantification were within the range of 0.03–0.24 and 0.26–0.75 μg/L, respectively. The intra- and inter-day precisions which were expressed in terms of the relative standard deviation ranged from 0.33 to 4.47 and 0.51–5.52%, respectively. The enrichment factors and recoveries ranged from 24 to 293 and 76–116%, respectively. The proposed method could be used simple, cheap, fast, and environmentally friendly alternative for analysis of organochlorine pesticides from environmental water and other similar matrices.

Monolithic capsule phase microextraction prior to gas chromatography-mass spectrometry for the determination of organochlorine pesticides in environmental water samples

In this study, a capsule phase microextraction (CPME) protocol followed by gas chromatography-mass spectrometry is proposed for the accurate and sensitive monitoring of organochlorine pesticides (OCPs) in environmental water samples. Different monolithic sol–gel encapsulated sorbents were compared and monolithic sol–gel poly(ethylene glycol)-based sorbent incorporated into porous microextraction capsules resulted in the highest extraction efficiency. Following the selection of the microextraction device, the CPME conditions were optimized, while linearity, limits detection (LODs), limits of quantification (LOQs), accuracy, and precision were the figures-of-merit measured. Under optimum conditions the LODs for the OCPs ranged between 0.01 ng mL−1 and 0.03 ng mL−1. The relative standard deviations were better than 5.3 % and 9.2 % for intra-day and inter-day study, respectively. The relative recoveries ranged between 90.5 and 105.2 % (intra-day) and 93.0–105.0 % (inter-day), demonstrating good method trueness. Finally, the proposed protocol was implemented for the monitoring of OCPs in tap, river, pond, and lake water. The developed method presents better analytical features than existing methods.

Miniaturized kapok fiber-supported liquid extraction for convenient extraction of pesticide residues in vegetable oils: Determination of organochlorine pesticides as a proof-of-concept study

In this paper, a miniaturized kapok fiber-supported liquid extraction (mini-KF-SLE) method was proposed for selective extraction of pesticide residues in vegetable oils. The natural kapok fiber was used as an inert oil support material based on its hydrophobic and lipophilic properties, and the extraction device was conveniently constructed by loading 15 mg of kapok fiber at the lower middle part of a 1-mL pipette tip. The vegetable oil sample (150 mg) without any pretreatment was directly loaded, followed by the addition of 150 μL of acetonitrile (ACN) as the extractant. After static extraction for 30 min, the extractant was pipetted out with a pipettor. As the proof of concept, it was applied for extracting eight organochlorine pesticides (OCPs) from vegetable oils and the eluate was analyzed by gas chromatography-electron capture detector (GC-ECD). Under optimized conditions, the extraction recoveries of OCPs were calculated to be in ranges of 35.8–79.5%. The satisfied quantitation ability was verified by the established method with coefficients of determination (R2) being greater than 0.99. The limits of detection (LODs) were in ranges of 2.0–50.0 ng/g. The relative recoveries were in ranges of 78.3–117.0% with the inter-/intra-day relative standard deviation (RSD) both being less than 13.3%. The potential of mini-KF-SLE to extract other kinds of pesticides was further verified by the successful extracting three triazole pesticides in vegetable oils with good extraction recoveries (>41.4%). The proposed mini-KF-SLE in combination with instrument detection techniques has the great potential in the low-cost and high-throughput determination of various pesticide residues in vegetable oils.

Bursting bubble flow microextraction combined with gas chromatography for determination of organochlorine pesticides in aqueous samples

Bubble-bursting flow microextraction (BBFME) is a simple and powerful microextraction method that conforms to the principles of green analytical chemistry (GAC). This method, combined with GC, is used for extraction, pre-concentration, and determination of some organochlorine pesticides (OCPs) in aqueous samples. In this method, the target analytes are transferred to the surface of the sample solution by the flow of gas bubbles. After the bubbles burst, the analytes disperse into the upper space and are finally collected and extracted. One of the unique properties of gas bubbles is the creation of a two-phase liquid–gas system, whereby the extraction process occurs at the liquid–gas interface where analytes interact with gas molecules in the bubble. In this study, a new method based on the availability of gas bubbles was proposed and investigated. The conditions affecting BBFME performance, including extraction time, the effect of ionic strength, and sample pH were investigated. Under optimal conditions, the dynamic linear range was 0.74–100.0 μg L-1, and the limit of detection and limit of quantification were 0.24–0.33 and 0.74–0.98 μg L-1, respectively. The range of enrichment factor and recovery under optimal conditions was between 228 and 273 and between 82.1 % and 98.2 %, respectively. Finally, using the BBFME method, the toxins simazine, γ-BHC, alachlor, aldrin and dieldrin were successfully extracted from various aqueous samples with satisfactory relative recoveries ranging from 95.3 to 105.1 %.

Analytical Determination of Organochlorine Pesticides in Soils and Foods

Owing to the widespread use of organochlorine pesticides in the past, significant amounts of them still occur in nature, mainly in the form of byproducts, which, however, often present a greater risk than the primary product used. Danger comes mainly from their ability to accumulate along the food chain and because they can enter the human body in different ways – through inhalation, penetration through the skin, through ingestion, etc. The current study on the content of this group of pesticides and their metabolites in soil and meat samples is an up-to-date development to analyse the potential hazards for environment and people. Eight soil samples and three types of meat foods were tested for the content of organochlorides and their metabolites. The results presented show that although these substances were found in some of the samples, their concentrations were below the regulatory permissible values.

Assessment of genotoxicity and oxidative stress in pregnant women contaminated to organochlorine pesticides and its correlation with pregnancy outcome

The present study was aimed to assess the correlation between transplacental transfer of xenobiotics and resulting biochemical alterations (including genotoxicity and oxidative stress) in non-occupational pregnant women of North India along with the effect on pregnancy outcomes. Maternal and cord blood samples were collected from 221 healthy mother-infant couples and divided according to their gestational age and birth weight. Genotoxic effects in mother and cord blood were examined using comet assay. The quantitative determination of Organo-chlorine pesticides in blood serum of study population was carried out using gas chromatography-mass spectrometry (GC-MS). Notably higher Organo-chlorine pesticides levels were observed in maternal blood of preterm than term subjects for almost all of the compounds detected, with the maximum concentration found for aldrin (3.26mg/l) in maternal blood and dieldrin (2.69mg/l) in cord blood. The results showed a significant increment in olive tail moment, tail full length, catalase, super-oxide dismutase, and malondialdehyde levels whereas lower glutathione reductase and peroxidase were found in preterm babies when compared with term group and it varied in the order: maternal blood>cord blood. A clear trend was observed for preterm babies with their lower birth weight and cesarean mode of delivery. Therefore, reduction in birth weight in newborns may be the consequence of increased oxidative damage and genotoxicity brought about by pesticides and these markers could be employed for early detection of pesticides related ailments and toxicities. To the best of our knowledge, this was a pioneering study and it may help to increase our knowledge with regard to xenobiotic exposure in biological system and the need for stringent guidelines for agricultural use of pesticides.

Effervescent Powder-Assisted Dispersive Liquid-Liquid Microextraction Using Floating Organic Solvents for Determination of Organochlorine Pesticides in Water by GC-MS

Effervescent Powder-Assisted Dispersive Liquid-Liquid Microextraction Using Floating Organic Solvents for Determination of Organochlorine Pesticides in Water by GC-MS

Utilization of montmorillonite nanocomposite incorporated with natural biopolymers and benzyl functionalized dicationic imidazolium based ionic liquid coated fiber for solid-phase microextraction of organochlorine pesticides prior to GC/MS and GC/ECD

A novel montmorillonite clay (MMT) bionanocomposite modified with chitosan (CH), carboxymethyl cellulose (CMC), and benzylimidazolium based dicationic ionic liquid with tetraethylene glycol linker (DIL) was fabricated on stainless steel wire by in situ process. The MMT–CH–CMC-DIL coated solid-phase microextraction (SPME) fiber was examined for the determination of organochlorine pesticides (OCPs) in real samples by HS-SPME-GC method using mass spectrometry (MS) and electron capture detector (ECD). Under optimized conditions, the proposed method exhibited low limits of detection (0.5 ng L−1 with MS and 0.1 ng L−1 with ECD detection), good linearities (R2 = 0.9972–0.9993 with MS and 0.9987–0.9998 with ECD detection), favorable single-fiber repeatability, and fiber-to-fiber reproducibility (less than 8.2% and 9.9% for both types of detection) and high reusability around 125 cycles. Recovery studies were carried out for OCPs in tap water, green tea, and milk samples to verify the applicability of the developed SPME-GC method.

Au@Ag nanoflowers based SERS coupled chemometric algorithms for determination of organochlorine pesticides in milk

In this study, SERS coupled with chemometric algorithms like PLS (partial least square), Si-PLS (synergy interval-PLS), GA-PLS (genetic algorithm-PLS) and UVE-PLS (uninformative variable elimination-PLS) were applied for the detection of 2,4-D and imidacloprid in milk. The fabricated gold and silver core-shell nanoflowers (Au@Ag NFs) were used as SERS nanosensor which exhibited strong signal over the concentration range of 1.0 × 10−3 to 1.0 × 102 ng/mL. Performances of the models were evaluated based on the attained correlation coefficients of the prediction (Rp) and calibration (Rc), root mean square error in calibration (RMSECV) and prediction (RMSEP), and the ratio of sample standard deviation to RMSEP (RPD). The models exhibited improved results in the order of PLS < Si-PLS < GA-PLS < UVE-PLS. The UVE-PLS model showed superior performance, yielded Rc = 0.9801 and Rp = 0.9878 with RPD = 6.32 for 2,4-D, and Rc = 0.9732 and Rp = 0.9726 with RPD = 4.30 for imidacloprid. The strategy could be employed for safety and quality monitoring of milk.

Development and Optimization of a Method for Organochlorine Pesticides Determination in Mussels Based on Miniaturized Matrix Solid-Phase Dispersion Combined with Gas Chromatography–Tandem Mass Spectrometry

Development and Optimization of a Method for Organochlorine Pesticides Determination in Mussels Based on Miniaturized Matrix Solid-Phase Dispersion Combined with Gas Chromatography–Tandem Mass Spectrometry

Dynamic microwave-assisted extraction combined with liquid phase microextraction based on the solidification of a floating drop for the analysis of organochlorine pesticides in grains followed by GC

A convenient, cost-effective and fast method using dynamic microwave-assisted extraction and liquid phase microextraction based on the solidification of a floating drop was proposed to analyze organochlorine pesticides in grains including rice, maize and millet. Twelve samples can be processed simultaneously in the method. During the extraction process, 10% acetonitrile-water solutions containing 110μL of n-hexadecane were used to extract organochlorine pesticides. Subsequently, 1.0g sodium chloride was placed in the extract, and then centrifuged and cooled. The n-hexadecane drops containing the analytes were solidified and transferred for determination by gas chromatography-electron capture detector without any further filtration or cleaning process. Limits of detection for organochlorine pesticides were 0.97–1.01μg/kg and the RSDs were in the range of 2.6%–8.5%. The developed technology has succeeded in analyzing six real grains samples and the recoveries of the organochlorine pesticides were 72.2%–94.3%. Compared with the published extraction methods, the developed method was used to analyze organochlorine pesticides in grains, being more environmentally friendly, which is suitable for the daily determination of organochlorine pesticides.