Growth Of Organisms Research Articles

Prediction of the Dissolved Oxygen Content in Aquaculture Based on the CNN-GRU Hybrid Neural Network

The dissolved oxygen (DO) content is one of the important water quality parameters; it is crucial for assessing water body quality and ensuring the healthy growth of aquatic organisms. To enhance the prediction accuracy of DO in aquaculture, we propose a fused neural network model integrating a convolutional neural network (CNN) and a gated recurrent unit (GRU). This model initially employs a CNN to extract primary features from water quality parameters. Subsequently, the GRU captures temporal information and long-term dependencies, while a temporal attention mechanism (TAM) is introduced to further pinpoint crucial information. By optimizing model parameters through an improved particle swarm optimization (IPSO) algorithm, we develop a comprehensive IPSO-CNN-GRU-TAM prediction model. Experiments conducted using water quality datasets collected from Eagle Mountain Lake demonstrate that our model achieves a root mean square error (RMSE) of 0.0249 and a coefficient of determination (R2) of 0.9682, outperforming other prediction models with high precision. The model exhibits stable performance across fivefold cross-validation and datasets of varying depths, showcasing robust generalization capabilities. In summary, this model allows aquaculturists to precisely regulate the DO content, ensuring fish health and growth while achieving energy conservation and carbon reduction, aligning with the practical demands of modern aquaculture.

Copper Ion-Chelated and Polydopamine-Modified Hydroxypropyl Methylcellulose Nanoparticles with Multiple Responses for Intelligent Pesticide Release and Improved Foliar Deposition.

Intelligent controlled-release nanopesticides have been a crucial tactic in advanced precision agriculture during the past few years, which can improve pesticide utilization and reduce environmental pollution. Herein, a novel hydroxypropyl methylcellulose-based nanopesticide carrier (PCH) with pH-/enzyme-/near-infrared multiple responses was constructed by the initial cross-linking with dimethyl diallyl ammonium chloride and the subsequent copper ion chelation and polydopamine coating. Avermectin (Av) was further loaded to create the intelligent pesticide release system (APCH) by antisolvent precipitation. The release of APCH increases under near-infrared light (NIR) conditions, with an accumulative release that is 3.26 times higher than that without NIR. The contact angles of APCH on cabbage leaves are 33 and 29% lower than those of water and technical Av (Av-tech), respectively, verifying that APCH has a good wettability property. Simultaneously, APCH displays better insecticidal activity than Av formulations because of its outstanding ultraviolet (UV) light stability of Av. The degradation rate of Av in APCH is less than 2% in 50 h under UV light, which is considerably lower than that of Av-tech (∼60%). The biosafety assessments manifest that the PCH carrier has remarkable biological safety on the growth of nontarget organisms in a certain range. Overall, the APCH system is bio-friendly with multiple-response release behaviors, excellent UV light stability, and foliar deposition performance, which can offer a new strategy for sustainable agricultural development.

Nutritional quality regulates postnatal wing morph in pea aphids

Aphids can produce winged or wingless offspring in response to environmental changes. Host nutrition is one of the extensively studied environmental factors influencing the plasticity of wing morphs of aphids. In this study, we found that the pea aphid, Acyrthosiphon pisum, produced a low proportion of winged offspring when fed on plants, but a significantly higher proportion on the artificial diet. Interestingly, when newly born nymphs were transferred back to the artificial diet after feeding on plants for six hours or longer, most nymphs became wingless. These results suggest that the wing morph state of pea aphids can change postnatally, potentially determined by the nutritional quality of their food. Furthermore, aphids feeding on the artificial diet exhibited higher levels of glucose and stronger insulin signaling activity compared with aphids on plants. Conversely, the amino acid levels were lower, and TOR signaling was weaker in aphids fed on the artificial diet. Insulin and the target of rapamycin (TOR) are the primary nutrient-sensing signaling pathways involved in controlling organism growth and have been implicated in regulating aphid wing morph plasticity. We tested whether these nutrient responsive pathways were involved in postanal wing determination of aphids. However, reducing amino acid content in the diet or inhibiting TOR with rapamycin resulted in a decrease of the winged morph, suggesting that the lower amino acid levels or TOR activity was not responsible for the higher proportion of winged morph on the artificial diet. These results suggest that nutritional quality, particularly sugars like sucrose and glucose, may regulate the postnatal wing morph of the pea aphid, likely via the insulin signaling pathway.

Assessing Environmental Risks of Local Contamination of Garden Urban Soils with Heavy Metals Using Ecotoxicological Tests

Heavy metal soil contamination in urban areas poses a significant environmental hazard, particularly in regions with historical or ongoing industrial activities. These areas are often polluted with metals such as Pb, Cu, Cd, and Zn, which can be absorbed by plants and pose risks to both ecosystems and human health. This study investigates soil contamination in urban gardens in Wroclaw, Poland, where elevated levels of trace elements were detected. Standard soil analyses, including macroelement content, granulometry, and trace element concentrations, were performed alongside an ecotoxicological evaluation using an Ostracodtoxkit test. The test evaluates the impact of contaminants on organism growth. An uncontaminated urban garden soil served as a reference. This study revealed that Zn, Cu, Pb, and Cd concentrations in soils exceeded limits permitted by Polish regulations in several soil samples. Despite the high concentrations of total metals, the bioavailable forms of these metals (measured by extraction of 1 M NH4NO3 extraction) were significantly lower, highlighting that the total metal content may not fully reflect the environmental risk. Pb was identified as the primary contributor to growth inhibition of test organisms, showing a particularly strong correlation with ecotoxicity. These findings underscore the importance of using ecotoxicological tests to evaluate soil contamination risks.

Utilization of Cassava Peel as Substrate for Production of Biofertilizer and Its Effect on the Growth of Millet

The quality of the carrier is critical in influencing microbial load and shelf life of biofertilizers. Cassava peels are abundant and have little economic value, making them ideal for use as biofertilizer carrier materials. The carrier material supported the growth of the test organism, thus suggesting the presence of nutrients and absence of toxicity. During isolation and culturing of bacteria, three (3) grams of soil sample was measured and diluted with 100ml of distilled water, and mixed well to get soil suspension. Ten (10) ml of the soil suspension was poured in the first test tube and shake well, 1ml of the first test tube was transferred into the second test tube containing nine (9) ml of sterile distilled water aseptically to get dilution. One (1)ml of the suspension from the second test-tube was transferred to third test tube also containing 9ml of sterile distilled water aseptically, 1ml of the soil suspension was transferred from the third test tube to the fourth test tube,1ml of the soil suspension was transferred from the fourth test tube was transferred to the fifth test tube,1ml of the soil suspension from the fifth soil suspension was also transferred to the sixth test tube, another 1ml from the sixth soil suspension was also transferred to seventh test tube aseptically. Soil sample from test tube 4, 5, and 6 was inoculated in yeast extract media and was incubated for 24 hours. The result shows that at 2DAP, 3DAP, 4DAP, 5DAP, AND 6DAP there is statistical significant difference between the treatment and control but at 7DAP, 8DAP, 9DAP AND 10DAP there is no statistical significant difference between the treatment and control but also at 11DAP there is statistical significant difference between the treatment and the control and at 10DAG, 12DAG, 14DAG, 16DAG, 18DAG, AND 20DAG there is no statistical significant difference between the treatment and control but at 22DAG there is statistical significant difference between the treatment and control at 24DAG there is no statistical significant difference between the treatment and the control. And at 26DAG there is statistical significant difference between the treatment and the control but also at 28DAG there is no statistical significant difference between the treatment and the control using fisher’s least significant difference test. Biofertilizer is a relevant alternative for disposal of this waste and even enables the act of converting wastes to wealth.

Navigating the Nano Abyss: Understanding the Ecological Ramifications of Nanoparticle Pollution on Aquatic Organisms

Increased environmental occurrences of nanoparticles are reported to be hazardous to aquatic life. The uptake of nano pollutants poses a significant impact on fish behaviour, reproductive function, endocrine system, and immune response, among other physiological factors. The review article highlighted various factors that are involved in nanotoxicity and also listed out the effects and mechanisms of nanoparticle on aquatic system. The behaviour and toxicity of nanoparticles on oxidative stress, DNA damage, and histology abnormalities are recorded from various literature. Additionally, the knowledge gaps on the effect of nanoparticles that could alter the neurological and immunological systems of aquatic creatures, influencing the release of cytokines and neurotransmitters are summarized. Various developmental defects and physiological disorders in fish are caused by mitochondrial malfunction, membrane damage, and DNA changes. Haematological, biochemical, and histological characteristics in the exposed microenvironment affect the natural growth and development of aquatic organisms. Overall, this review highlights the critical information on the toxicity of the nano exudate on the aquatic species and records of their ecotoxicological assessment of aquatic ecosystem.

The Biotechnological Potential of Crickets as a Sustainable Protein Source for Fishmeal Replacement in Aquafeed.

As aquaculture production grows, so does the demand for quality and cost-effective protein sources. The cost of fishmeal (FM) has increased over the years, leading to increased production costs for formulated aquafeed. Soybean meal (SBM) is commonly used as an FM replacer in aquafeed, but anti-nutritional factors could affect the growth, nutrition, and health of aquatic organisms. Cricket meal (CM) is an alternative source with a nutrient profile comparable to FM due to its high protein content, digestibility, and amino acid profile. CM use in aquafeed influences growth and reproductive performance while modulating the gut microbiota and immune response of fish and shrimp. However, consistent regulation and scaling up are necessary for competitive prices and the marketing of CM. Moreover, the chitin content in CM could be an issue in some fish species; however, different strategies based on food biotechnology can improve the protein quality for its safe use in aquafeed.

Temperature and feeding frequency: interactions with growth, immune response, and water quality in juvenile Nile tilapia

BackgroundWater temperature and feeding frequency are critical abiotic factors regulating the growth and immune function of aquatic organisms. This study investigated the effects of water temperature and feeding frequency on growth and immune function in Nile tilapia (Oreochromis niloticus) over two months. A total of 360 juvenile fish (average weight: 20.00 ± 1.26 g) were divided into six groups, each with three replicates, based on a combination of three water temperatures (26, 28, and 30 °C) and two feeding frequencies (either 1 or 2 meals per day).ResultsAt 30 ºC and 28 ºC, water electrical conductivity and total dissolved salts increased, while total ammonia nitrogen and dissolved oxygen rose slightly in groups fed twice daily, with a significant interaction between temperature and feeding frequency. The group at 30 ºC with two meals per day showed the highest final body weight (FBW). The interaction between temperature and feeding frequency significantly influenced FBW, total feed intake, and body thickness. Fish at 30 ºC exhibited upregulated hepatic growth hormone receptor 1 and insulin-like growth factor 1, while those at 28 ºC with one meal per day, as well as those at 30 ºC regardless of meal frequency, also showed increased expression of hepatic fatty acid binding protein and intestinal cluster of differentiation 36. Fish at 30 ºC had upregulated leptin levels and downregulated cholecystokinin, while those at 26 ºC displayed the opposite trend, particularly with one meal daily. Higher temperatures significantly boosted serum IgM, superoxide dismutase (SOD), and lysozyme (LYZ) levels, with meal frequency also affecting malondialdehyde, IgM, and SOD levels. Additionally, 30 ºC enhanced the hepatic expression of mucin-like protein (muc), oligo-peptide transporter 1 (pept1), interleukin 1, nf-κB, complement C3, lyz, sod, catalase, and glutathione peroxidase, with twice-daily meals having a more pronounced effect. Conversely, 28 ºC with one meal per day upregulated some of these genes, such as muc, pept1, and sod.ConclusionsOverall, 30 ºC with two meals per day significantly improved the growth and health of juvenile Nile tilapia, while 28 ºC with two meals maintained satisfactory performance.

A Novel Methodological Approach to Simulating the Growth of Photosynthetic Organisms Using Long-Term Meteorological Sequences: A Case Study of Microalgae (Chlorella vulgaris)

The growth of photosynthetic organisms requires specific ranges of temperature and photosynthetically active radiation. Monitoring and maintaining these conditions is technically difficult, especially in outdoor cultures. In such cases, a typical meteorological sequence can be a useful tool for estimating the growth of photosynthetic organisms. This study proposes a new methodology based on long-term meteorological sequences to simulate the growth of photosynthetic organisms. This case study addresses microalgae growth simulation (Chlorella vulgaris) in Riosequillo in the north of the Madrid region (Spain) for the four seasons of the year. Then, these estimates are compared with the observed results of an experimental culture of microalgae in domestic wastewater. The results also show strong agreement with the probability distribution function of the daily biomass concentration, giving the best results for typical summer and spring meteorological sequences. The methodology seems to confirm the representativeness of typical meteorological sequences, allows for the identification of the most likely production scenarios for project feasibility analyses, and may be applied to decision-making processes.

An Ecologically Consistent Model of Growth for Hard-Bodied Marine Organisms

There are several factors to account for marine growth including but not limited to temperature, salinity, chlorophyll-a content, existing species in the environment and predating. This paper proposes a model of biological growth for hard species on marine structures, which can be compatible with site-specific and realistic ecology while also being able to translate the results for analyses linked to lifetime hydrodynamic or structural effects via commercial software or computing. The model preserves fundamentals of ecological aspects rather than using heuristics or random sampling to data fitting on sparsely collected information. The coefficients used in the proposed model align to the real world, with location-specific values, and can be adapted to new information. The growth model is demonstrated for Mythulis Edulis (blue mussel) colonisation to assess the lifetime hydrodynamic effects for the West Coast of Ireland and the Gulf of Guinea. The model can be extended to any hard growth approach.

Junk DNA: Is it Really Junk - or a Code Waiting to Be Cracked?

The idea that our genetic material contains Junk DNA is fading. Many DNA segments that were previously believed to be worthless now have important functions, according to recent studies. These non-coding areas, once known as Junk DNA, are crucial for regulating when and how genes function even though they do not directly make proteins. They assist our cells respond to various circumstances and direct the growth of organisms. Certain non-coding DNA sequences function as switches that determine where and when genes should be activated or deactivated. Furthermore, some non-coding DNA segments help maintain the orderly structure of our chromosomes. They also contribute to the evolution of species and may contain remnants of ancient viruses that once infected our forebears. Although scientists still don’t fully understand every aspect of non-coding DNA, it is now recognized as a complex and active element of our biology. Researchers believe that what was once labelled as Junk DNA could actually be very important for our health, development, and ability to adapt to environmental changes. This new perspective is helping us understand how valuable Junk DNA really is.

Recent Advancements in Harnessing Lactic Acid Bacterial Metabolites for Fruits and Vegetables Preservation.

Postharvest losses in fruits and vegetables exert substantial economic and environmental repercussions. Chemical interventions are being widely utilized for the past six decades which may lead to significant health complications. Bioprotection of fruits and vegetables is the need of the hour in which use of lactic acid bacteria (LAB) with GRAS status predominantly stands out. Incorporation of LAB in postharvest fruits and vegetables suppresses the growth of spoilage organisms by synthesizing various antimicrobial compounds such as bacteriocins, organic acids, hydrogen peroxide (H2O2), exopolysaccharides (EPS), and BLIS. For example, Pediococcus acidilactici, Lactobacillus plantarum, and Limosilactobacillus fermentum convert natural sugars in fruits and vegetables to lactic acid and create an acidic environment that do not favour spoilage organisms. LAB can improve the bioavailability of vitamins and minerals and enrich the phenolic profile and bioactivity components. LAB has remarkable physiological characteristics like resistance towards bacteriophage, proteolytic activity, and polysaccharide production which adds to the safety of foods. They modify the sensory properties and preserve the nutritional quality of fruits and vegetables. They can also perform therapeutic role in the intestinal tract as they tolerate low pH, high salt concentration. Thus application of LAB, whether independently or in conjunction with stabilizing agents as edible coatings, is regarded as an exceptionally promising methodology for ensuring safer consumption of fruits and vegetables. This review addresses the most recent research findings that harness the antagonistic property of lactic acid bacterial metabolites, formulations and coatings containing their bioactive compounds for extended shelf life of fruits and vegetables.

Unusual modes of cell and nuclear divisions characterise Drosophila development.

The growth and development of metazoan organisms is dependent upon a co-ordinated programme of cellular proliferation and differentiation, from the initial formation of the zygote through to maintenance of mature organs in adult organisms. Early studies of proliferation of ex vivo cultures and unicellular eukaryotes described a cyclic nature of cell division characterised by periods of DNA synthesis (S-phase) and segregation of newly synthesized chromosomes (M-phase) interspersed by seeming inactivity, the gap phases, G1 and G2. We now know that G1 and G2 play critical roles in regulating the cell cycle, including monitoring of favourable environmental conditions to facilitate cell division, and ensuring genomic integrity prior to DNA replication and nuclear division. M-phase is usually followed by the physical separation of nascent daughters, termed cytokinesis. These phases where G1 leads to S phase, followed by G2 prior to M phase and the subsequent cytokinesis to produce two daughters, both identical in genomic composition and cellular morphology are what might be termed an archetypal cell division. Studies of development of many different organs in different species have demonstrated that this stereotypical cell cycle is often subverted to produce specific developmental outcomes, and examples from over 100 years of analysis of the development of Drosophila melanogaster have uncovered many different modes of cell division within this one species.

Biomimetic Folding Strategies for Chemical Synthesis of Disulfide-Bonded Peptides and Proteins.

Disulfide-bonded peptides and proteins, including hormones, toxins, growth factors, and others, are abundant in living organisms. These molecules play crucial physiological roles such as regulating cell and organism growth, development, and metabolism. They have also found widespread applications as drugs or tool molecules in biomedical and pharmaceutical research. However, the chemical synthesis of disulfide-bonded proteins is complicated by the challenges associated with their folding. This review focuses on the latest advancements in disulfide-bonded peptide and protein folding technologies. Particularly, it highlights biomimetic folding strategies that emulate the naturally occurring oxidative folding processes in nature. These strategies include chaperone-assisted folding, glycosylation-assisted folding, and organic-based oxidative folding methods. The review also anticipates future directions in folding technology. Such research offers innovative approaches for the chemical synthesis of complex proteins that are otherwise difficult to fold.

Vitamin C Alleviates Heat-Stress-Induced Damages in Pig Thoracic Vertebral Chondrocytes via the Ubiquitin-Mediated Proteolysis Pathway.

Heat stress can impair organismal growth by inducing ubiquitination, proteasome-mediated degradation, and subsequent cellular damage. Vitamin C (VC) has been shown to potentially mitigate the detrimental effects of abiotic stresses on cells. Nevertheless, the impact of heat stress on growth plate chondrocytes remains unclear, and the underlying protective mechanisms of VC in these cells warrant further investigation. In this study, we focused on pig thoracic vertebral chondrocytes (PTVCs) that are crucial for promoting the body's longitudinal elongation and treated them with 41 °C heat stress for 24 h, under varying concentrations of VC. Our findings reveal that, while oxidative stress induced by heat triggers apoptosis and inhibits the ubiquitin-mediated proteolysis pathway, the addition of VC alleviates heat-stress-induced oxidative stress and apoptosis, mitigates cell cycle arrest, and promotes cellular viability. Furthermore, we demonstrate that VC enhances the ubiquitin-proteasome proteolysis pathway by promoting the expression of ubiquitin protein ligase E3A, which thereby stabilizes the ubiquitin-mediated degradation machinery, alleviates the apoptosis, and enhances cell proliferation. Our results suggest the involvement of the ubiquitin-mediated proteolysis pathway in the effects of VC on PTVCs under heat stress, and offer a potential strategy to make use of VC to ensure the skeletal growth of animals under high temperature pressures in summer or in tropical regions.

NARIKELA PUSHPA CHOORNA IN THE MANAGEMENT OF PITTAJA MOOTRAKRICHRA WITH SPECIAL REFERENCE TO NON-SPECIFIC URINARY TRACT INFECTION: A CASE STUDY

Pittaja mootrakrichra is one among the eight types of mootrakrichra, which are having a signs and symptoms as that of Urinary tract infection. UTI is the fifth most common infectious diseases, accounts more than 9.5% of infections. The non-specific UTI is usually caused by Escherichia coli, which is derived from the intestinal flora. Less frequently caused by other organisms like Streptococci, Klebsiella, Enterobacter, etc. In Ayurveda, Mootrakrichra disease, its treatment and formulations are well explained, which are having a very good results in treating the disease from its root causes. One among such formulations is Narikela pushpa. The present case study is of 50 years old female subject reported to the OPD of Shri DGM Ayurvedic Medical College and Hospital, Gadag, Karnataka, India, with the signs and symptoms of Pittaja mootrakrichra and laboratory investigations with positive Escherichia coli organism growth in the urine culture. Hence, Narikela pushpa choorna medication is planned in the management of Pittaja mootrakrichra.

GEMAS: Boron as a geochemical proxy for weathering of European agricultural soil

About a century ago, B was recognised as an essential element for the normal growth of plants and terrestrial organisms. Limitations for plant development have been recognised in agricultural systems, particularly in highly weathered soil. Boron is rarely analysed in whole rock or soil analysis, as it requires specific analytical techniques. It is often determined, after partial extraction (aqua regia or CaCl), usually on a limited number of samples. Many more questions than answers exist about the environmental behaviour of B.We present B contents in agricultural soil samples (0–10 cm) collected in 33 European countries (5.6 million km2) during the GEMAS (GEochemical Mapping of Agricultural and grazing land Soil) continental-scale project. The B content, determined by ICP-MS following hot aqua regia extraction, varies in European agricultural soil from 0.5 to 49 mg/kg (median 2.42 mg/kg, n = 2108), which is somewhat similar to total B estimates for the Upper Continental Crust (17–47 mg/kg). Its spatial distribution in agricultural soil shows a patchy pattern with low values in regions with granitic bedrock and high contents in soil formed over limestone and in volcanic areas.Boron geochemical behaviour in soil is strongly dependent on other factors such as pH, CEC, presence of organic matter, clay and secondary oxides and hydroxides. Boron geochemical mapping at the continental scale in arable soil allows investigations of plant health, i.e., the beneficial and adverse effects due to the nutritional status of boron.

Impact of Exogenous Lactiplantibacillus plantarum on the Gut Microbiome of Hematopoietic Stem Cell Transplantation Patients Colonized by Multidrug-Resistant Bacteria: An Observational Study.

Background:Lactiplantibacillus plantarum can inhibit the growth of multidrug-resistant organisms (MDROs) and modulate the gut microbiome. However, data on hematopoietic stem cell transplantation (HSCT) are scarce. Aim: In an observational study, we assessed the impact of L. plantarum on the modulation of the gut microbiome in HSCT patients colonized by MDROs. Methods: Participants were allocated to an intervention group (IG = 22) who received capsules of L. plantarum (5 × 109 CFU) twice per day until the onset of neutropenia or a control group (CG = 20). The V4 region of the 16S bacterial rRNA gene was sequenced in 87 stool samples from a subset of 33 patients (IG = 20 and CG = 13). The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) program was used to predict metagenome functions. Results:L. plantarum demonstrated an average 86% (±11%) drug-target engagement at 43 (±29) days of consumption and was deemed safe, well-tolerated, and associated with an increase in the abundance of the Lactobacillales (p < 0.05). A significant increase in Lactococcus and a reduction in Turicibacter (p < 0.05) were identified on the second week of L. plantarum use. Although Enterococcus abundance had a greater rise in the CG (p = 0.07), there were no significant differences concerning the Gram-negative MDROs. No serious adverse effects were reported in the IG. We observed a greater, non-significant pyruvate fermentation to propanoate I (p = 0.193) relative abundance in the IG compared with the CG. L. plantarum use was safe and tolerable by HSCT patients. Conclusions: While L. plantarum is safe and may impact Enterococcus and Turicibacter abundance, it showed no impact on Gram-negative MDRO abundance in HSCT patients.

Unraveling the molecular mechanism of FgGcn5 inhibition by phenazine-1-carboxamide: combined in silico and in vitro studies.

Fusarium head blight (FHB), mainly caused by Fusarium graminearum (F. graminearum), remains a devastating disease worldwide. The histone acetyltransferase Gcn5 plays a crucial role in epigenetic regulation. Aberrant Gcn5 acetylation activity can result in serious impacts such as impaired growth and development in organisms. The secondary metabolite phenazine-1-carboxamide (PCN) inhibits F. graminearum by blocking the acetylation process of Gcn5 (FgGcn5), and is currently used to control FHB. However, the molecular basis of acetylation inhibition by PCN remains to be further explored. Our molecular dynamics simulations revealed that PCN binds to the cleft in FgGcn5 where histone H3 is bound, with key amino acid residues including Leu96 (L96), Arg121 (R121), Phe133 (F133), Tyr169 (Y169), and Tyr201 (Y201), preventing FgGcn5 from binding to histone H3 and affecting histone H3 from being acetylated. Experimental validation of key amino acid mutations further confirmed the impact of these mutations on the interaction of FgGcn5 with PCN and histone H3 peptide. In summary, our study sheds light on the mechanism by which PCN inhibits the acetylation function of FgGcn5, providing a foundation for the development of drugs or fungicides targeting histone acetyltransferases. © 2024 Society of Chemical Industry.

Combined toxic effects of yessotoxin and polystyrene on the survival, reproduction, and population growth of rotifer Brachionus plicatilis at different temperatures.

Yessotoxin (YTX) is a disulfated toxin produced by harmful dinoflagellates and causes risks to aquatic animals. Polystyrene (PS) microplastics could absorb toxins in seawaters but pose threats to organism growth. In this study, the combined toxic effects of YTX (0, 20, 50, and 100 µg L-1) and PS (0, 5, and 10 µg mL-1) on the survival, reproduction, and population growth of marine rotifer Brachionus plicatilis at 20 °C, 25 °C, and 30 °C were evaluated. Results indicated that the survival time (S), time to first batch of eggs (Ft), total offspring per rotifer (Ot), generational time (T0), net reproduction rate (R0), intrinsic growth rate (rm), and population growth rate (r) of rotifers were inhibited by YTX and PS at 25 °C and 30 °C. Low temperature (20 °C) improved the life-table parameters T0, R0, and rm at YTX concentrations less than 100 µg L-1. Temperature, YTX, and PS had interactive effects on rotifers' S, Ft, Ot, T0, R0, rm, and r. The combined negative effects of YTX and PS on rotifers' survival, reproduction, and population growth were significantly enhanced at 30 °C. These findings emphasized the importance of environmental temperature in studying the interactive effects of microplastics and toxins on the population growth of zooplankton in eutrophic seawaters.