Organic-walled Dinoflagellate Cysts Research Articles

Dinoflagellate cysts as proxies of environmental, ocean and climate changes in the Atlantic realm during the quaternary

Over the last four decades, organic-walled dinoflagellate cysts have shown high potential as tracers of past sea-surface conditions during the Quaternary. These microfossils relate to the pelagic productivity of both phototrophic and heterotrophic protist organisms and are recovered in high numbers in almost all marine environment settings from the nearshore and estuarine systems to the distal continental margin. In polar environments, where other conventional proxies are rare or absent, dinoflagellate cysts showed a relatively high diversity of species and a close relationship with sea-ice cover duration, winter and summer temperature, and salinity, enabling quantitative reconstructions of several oceanic variables over time. From the temperate to the tropical latitudes of the Atlantic Ocean, their modern distribution highlights a response to primary productivity and seasonal contrasts in surface temperature. They also have proven that they could be used as tracers of eutrophication in stratified systems and can also highlight human impact on their distribution. In this paper, we present an overview of dinoflagellate cysts as ecological tracers in recent and past sediments of the Atlantic Ocean, from the Arctic to the Antarctic. We provide examples of their use as proxies in paleoclimatic-palaeoceanographic studies at glacial to interglacial time scales, with emphasis on the last ice age to recent (last 25 kyr), the northern North Atlantic and western-eastern tropic North Atlantic. We also discuss their potential as tracers of anthropogenic stress in coastal environments.

Transport and preservation of calcareous and organic-walled dinoflagellate cysts off Cape Blanc (NW africa) in relation to nepheloid layers

Our understanding of dinoflagellates' present-day and past ecology is limited due to the scarcity of data on the transport of dinoflagellate cysts in oceanic environments. Previous studies have shown that lateral transport affects the source-to-sink trajectory of cysts in the very productive region off Cape Blanc (NW Africa). Unsolved questions remain, such as: how far these cysts can be advected, whether the cyst sources vary over time and whether lateral transport is a permanent feature or restricted to individual events. To fill these gaps and assess the role of nepheloid layers on the lateral transport and preservation of dinoflagellate cysts, new data on dinoflagellate cyst distributions in the water column and sediments along a land-sea transect were obtained.Samples were collected in November 2018 along a shelf break-offshore transect during intense upwelling, notably, within and between the nepheloid layers. The composition and abundance of cysts with organic walls in the water column and surface sediments were studied. Moreover, the distribution of calcareous cysts was also analysed in the water samples, using non-destructive acid-free preparation methods. The records were dominated by empty cysts, but no clear indications that these originated from local resuspension of older sediments were observed. Clustering, principal component analysis and redundant discriminant analysis were used to compare cyst assemblages in the water column and surface sediments, and environmental conditions in the upper water column. The strong similarity in species composition of water samples collected in the active upwelling region to those collected from the more onshore parts of the Benthic Nepheloid Layer (BNL), upper Intermediate Nepheloid Layer (INL) (∼1000 m depth) and lower INL (∼2200 m depth) indicated that lateral transport of cysts within these NLs occurred until about ∼110 km from the shelf break. Cyst assemblages from above and below these NLs showed significantly different taxa composition reinforcing the role of NLs in the lateral advection of cysts. In the more offshore stations, vertically similar cyst assemblages were observed in the same station, independent of the sample depth, within or between the NLs, which supported that at these stations vertical transport was the dominant process influencing cyst assemblages. Consequently, the cyst signal in sediments off Cape Blanc may be affected both by horizontal transport of allochthonous cysts and vertical deposition of locally-produced cysts, particularly in the more offshore stations (>2000 m depth). Despite lateral transport and possible species-specific preservation effects, horizontal distributions of most cyst taxa in the water column and the surface sediments could be explained to a great extent by the main environmental gradients in the upper water column. This agrees with observations made in other regions, and reinforces that dinoflagellate cysts as good proxies to reconstruct past environmental conditions in offshore environments. New data on dinoflagellate cyst distribution, transport and accumulation patterns in deep environments off Cape Blanc may be useful for interpreting past environmental signals in the region. This is particularly relevant regarding calcareous cysts, as information on their distribution and ecology is very scarce. The present work contributes to a better understanding of the dispersal patterns of dinoflagellate cysts in the deep ocean, highlighting the significant role played by nepheloid layers in this process and thus on the dinoflagellate cyst signature in deep-sea sediments.

Environmental control of interannual and seasonal variability in dinoflagellate cyst export flux over 18 years in the Cape Blanc upwelling region (Mauritania)

The increasing threat of anthropogenic environment and climate change amplifies the urgency to investigate the effect of these changes on marine ecosystems. We provide information about the export flux of organic-walled dinoflagellate cysts between 2003 and 2020 in the upwelling ecosystem off Cape Blanc (Mauritania), one of the world’s most productive regions. We compared the cyst export flux with variability in environmental parameters, such as wind speed, wind direction, dust emission, sea surface temperature (SST), SST difference between trap location and open ocean (SSTa), and chlorophyll-a concentration. This information is valuable to determine the ecological signal of dinoflagellate cysts that could be applied in recent and paleo records. The total export production of dinoflagellate cysts fluctuated between 0 - 1.18 x 105 cysts m-2 d-1 for the heterotrophs and 0 - 1.06 x 104 cysts m-2 d-1 for the photo-/mixotrophs. The export productions of both groups were in line with changes in upwelling intensity, which in most years, intensified in spring - summer. Dinoflagellate cyst association was dominated by heterotrophic taxa that formed an average of 94% of the association throughout the sediment trap record. A strong interannual variation in the cyst export fluxes, as well as the association composition was observed in the record. We identified five groups that showed comparable variability in export production with changes in environmental conditions: (1) maximal upwelling; Echinidinium delicatum/granulatum, E. transparantum/zonneveldiae, Echinidinium spp., Trinovantedinium spp., and Protoperidinium latidorsale, (2) combined maximal upwelling and dust input; Archaeperidinium spp., P. americanum, P. stellatum, and P. subinerme, (3) upwelling relaxation; Gymnodinium spp. and L. polyedra, (4) warm surface waters; Bitectatodinium spongium and Protoceratium reticulatum, (5) species with no specific relationship to the studied environmental variables; Brigantedinium spp., E. aculeatum, Impagidinium aculeatum, P. conicum, P. monospinum, Pentapharsodinium dalei, and Spiniferites spp. The sediment trap record documented a gradual shift in the cyst taxa association that co-occurred with the gradual increase of Saharan dust input to the region, notably after 2008. The cyst association contained five photo-/mixotrophic taxa that were formed by potentially toxic dinoflagellates. The latter could cause threats to the socio-economy of coastal communities.

The Jurassic-Cretaceous boundary on the northern Tethyan margin: Karpentná and Ropice sections (Outer Western Carpathians, Czech Republic)

Microfacies and high-resolution studies at the Silesian Unit (Outer Western Carpathians, Czech Republic) on calpionellids, calcareous and non-calcareous dinoflagellate cysts, foraminifers and calcareous nannofossils, aligned with paleomagnetism, δ13Ccarb and δ18Ocarb, allow construction of a detailed stratigraphy and palaeoenvironmental interpretations across the Jurassic-Cretaceous (J/K) boundary. Two studied sections consist of allodapic and biomicritic limestones and marlstones. Ropice section spans the stratigraphic range from the upper Tithonian calpionellid Chitinoidella Zone, nannoplankton zone NJT16 to the lower Berriasian calpionellid Alpina Subzone of the Calpionella Zone, nannoplankton NC1 Zone. The J/K boundary is marked by the dominance of small forms of Calpionella alpina, rare occurrence of Nannoconus wintereri, and the first occurrence of organic-walled dinoflagellate cysts Dichadogonyaulax bensonii and Muderongia longicorna. A negative shift in the δ13Ccarb values corresponds to the Tithonian/Berriasian boundary, the base of the Calpionella Zone (Alpina Subzone). Karpentná section is represented by upper Tithonian calpionellid Chitinoidella to Crassicollaria zones. Remagnetization was documented in both sections.

Two new ceratioid cornucavate dinoflagellate cysts from the Upper Cretaceous, Gulf of Suez, Egypt

Diverse and well-preserved assemblages of organic-walled dinoflagellate cysts have been recovered from the Matulla Formation (Coniacian – Santonian), Gulf of Suez, Egypt. Among the abundant dinoflagellate cyst assemblages, the new species Odontochitina elbeialyi sp. nov. and Odontochitina ornata sp. nov. (Family Ceratiaceae) are described. The two new species are unique by having a granulate periphragm and three prominent horns. The horns are truncated distally and devoid of striae, ridges and perforations. Odontochitina elbeialyi sp. nov. is characterized by a weakly expressed tabulation on the pericyst indicated by the occasional presence of sutural lines or low ridges indicating the cingulum (two transverse ridges), at plate boundaries. Odontochitina ornata sp. nov. differs from the former, particularly by its clear tabulation, which is corniform gonyaulacoid and more strongly expressed by sutural ridges, and by rare short (rudimentary) sutural (mostly gonal) nipple-like, to truncated processes. Based on the current material, Odontochitina elbeialyi sp. nov. and Odontochitina ornata sp. nov. have short stratigraphic ranges and may therefore be considered as stratigraphic markers for the late Santonian (Late Cretaceous). Their comparison with all formally published species of the genus Odontochitina is presented and discussed, and the associated relevant dinoflagellate cyst taxa are also indicated. The paleoenvironmental significance of the Odontochitina elbeialyi sp. nov. and Odontochitina ornata sp. nov. is discussed.

Dual nomenclature in organic-walled dinoflagellate cysts I: concepts, methods and applications

Dual nomenclature in dinoflagellates is supported under the current nomenclatural code for algae, fungi and plants and allows a fossil-defined (usually cyst) species to bear a name other than that of its equivalent non-fossil species, as established for example by incubation experiments. Two names can then apply to the same cyst morphotype, reflecting the separate but overlapping concepts and criteria used for fossil- and non-fossil taxa. Fossil-species are normally and logically assigned to fossil-genera and non-fossil species to non-fossil genera, a practice that facilitates dual nomenclature. Inconsistencies and ambiguities arise when binomials combine the names of fossil- with non-fossil taxa. Examples of this hybridised nomenclature and its consequences are examined, with problems identified and potential solutions discussed. Accordingly, a new non-fossil genus Lingulaulax is proposed with Lingulaulax polyedra (von Stein 1883) comb. nov. as its type and equivalent to the fossil-species Lingulodinium machaerophorum (Deflandre & Cookson 1955) Wall 1967, along with the new combination Lingulaulax milneri (Murray & Whitting 1899); the genus Lingulodinium Wall 1967 is retained in its exclusively fossil status. The non-fossil name Gonyaulax ellegaardiae Mertens et al. 2015 is validly published herein.

Palsys.org: an open-access taxonomic and stratigraphic database of organic-walled dinoflagellate cysts

Abstract. It is with great pleasure that we introduce palsys.org (https://palsys.org/genus/, last access: 8 December 2023), a fully open-access taxonomic, stratigraphic and image database of organic-walled dinoflagellate cysts. Palsys.org started as the in-house database of the Laboratory of Palaeobotany and Palynology (LPP) Foundation over 30 years ago. It is now owned by Utrecht University and has been expanded and transformed into a public online platform for use in research and education. Palsys.org includes the taxonomic descriptions of genera and species of organic walled dinoflagellate cysts, from the (often translated) literature, and emendations and synonymy, mainly following Williams et al. (2017) and the stratigraphic calibrations from DINOSTRAT (Bijl, 2022), and has around 25 000 images of species. Here, in this launch paper, we explain the history of the database, present its current functionalities and explain our set-up of the data quality control. We call upon the community to help us keep palsys.org up to date and complete by, for example, by sending additional information, imagery and feedback in general through the platform. Palsys.org brings dinoflagellate micropaleontology in line with the open-science principles of modern academia.

New physical and biological evidence of lateral transport affecting dinoflagellate cyst distribution in the benthic nepheloid layer along a land-sea transect off Figueira da Foz (Atlantic Iberian margin)

IntroductionThe production of resting cysts is a key dispersal and survival strategy of many dinoflagellate species. However, little is known about the role of suspended cysts in the benthic nepheloid layer (BNL) in the initiation and decline of planktonic populations.MethodsIn September 2019, sampling of the dinoflagellate cyst community at different water depths in the water column and in the bottom sediments, and studies of spatio-temporal changes in physical properties (temperature, salinity, density and suspended sediment concentration), were carried out along a land-sea transect off Figueira da Foz (NW Portugal) to investigate the dinoflagellate cyst distribution and the factors (physical and biological) affecting it. A clustering analysis was used to compare the BNL and sediment cyst records with the cyst rain recorded by a sediment trap at a fixed station. Furthermore, Lagrangian particle experiments enabled simulating cyst trajectories in the BNL 5 and 10 days before sampling and assessing cross-shore, vertical and alongshore transport within the studied region.ResultsA well-developed BNL was present during the survey, which covered a change from active (14th of September) to relaxed (19th of September) upwelling conditions. Organic-walled dinoflagellate cysts were dominant in all samples, although calcareous dinoflagellate cysts consistently occurred (at low abundances). High proportions of full cysts were observed in the BNL, of which a significant portion was viable as shown by excystment experiments. Moreover, BNL cyst records collected on the 19th of September along the land-sea transect were similar to the sediment trap cyst record but greatly differed from sediment cyst records. The heterotrophic small spiny brown cysts (SBC) and cysts of the autotrophic yessotoxin-producer Protoceratium reticulatum notably increased during the survey, in the BNL and in the water column above.DiscussionThe comparison of the BNL, surface sediment and sediment trap cyst records supported that the main origin of cysts in the BNL was the recent production in the water column. The spatial coincidences in the distribution of cysts and vegetative cells of Protoceratium reticulatum also supported that full cysts in the water column were being produced in surface waters. New data evidenced the presence of a significant reservoir of viable cysts in the BNL that have the potential to seed new planktonic blooms. Furthermore, back-track particle modelling evidenced that alongshore advection was the main physical mechanism controlling cyst dynamics in the BNL during most part of the survey period, being particularly intense in coastal stations (<100 m depth). Consequently, the sediment cyst signal is a mixture of locally and regionally produced cysts. We provide multi-disciplinary data evidencing that cysts recently formed in the photic zone can be laterally advected within the studied region through the BNL, contributing to a better understanding of the role of the BNL in cyst dynamics and tracing the seed sources of the new blooms.

Disentangling environmental drivers of subarctic dinocyst assemblage compositional change during the Holocene

Abstract. Analysis of compositional changes in fossil organic-walled dinoflagellate cyst (dinocysts) assemblages is a widely used tool for the quantitative reconstruction of past environmental parameters. This approach assumes that the assemblage composition is significantly related to the reconstructed environmental parameter, which requires an independent correlation between the assemblage and the variable, meaning that the variable explains a dimension of the assemblage variance that is not also explained by other parameters. Theoretically, dinocyst assemblages can be used to reconstruct multiple environmental variables. However, there is evidence that primary and subordinate drivers for assemblage compositions regionally differ, and it remains unclear whether a significant relationship to specific parameters in the present ocean always implies that this relationship is significant in fossil assemblages, questioning if past changes in these multiple parameters can be reconstructed simultaneously from fossil assemblages. Here, we analysed a local subset of the Northern Hemisphere dinocyst calibration dataset (n=1968), including samples from the Baffin Bay area (n=421), and evaluated the benefits of a local versus a more regional or global calibration for the environmental reconstruction of Baffin Bay oceanography during the Holocene. We determined the dimensionality of the dinocyst ecological response and identified environmental drivers in the Baffin Bay area for the modern dataset. We analysed four existing Holocene records along a north–south transect in the area and evaluated the statistical significance of downcore reconstructions by applying the local and global datasets with different techniques: the modern analogue technique (MAT), the weighted average partial least square (WA-PLS) and maximum likelihood (ML). We evaluated reconstructions tested as significant in light of the existing state of knowledge about West Greenland's Holocene palaeoceanography. Our analyses imply that present-day and Holocene dinocyst assemblages in the Baffin Bay area are primarily driven by salinity changes; other parameters were less important in driving assemblage compositions, and their contribution differed among the studied records. We show that the objectively occurring and temporally coherent shifts in dinocyst assemblages in the Holocene of Baffin Bay can be robustly interpreted only by transfer functions that are locally calibrated. Transfer functions based on the broad Northern Hemisphere calibration yielded many insignificant environmental reconstructions. At the same time, we show that even in the local calibration, not all parameters that appear to significantly affect dinocyst assemblages in the calibration dataset can be meaningfully reconstructed in the fossil record. A thorough evaluation of the calibration dataset and the significance of downcore applications is necessary to reveal the region-specific information contained in dinocyst assemblage composition.

Integrated biostratigraphy (orbitolinids, calcareous nannofossils, and palynomorphs) of the Lower Cretaceous Sarcheshmeh Formation, western Koppeh-Dagh Basin, NE Iran

An integrated biostratigraphy using orbitolinids, calcareous nannofossils, and dinoflagellate cysts was conducted on the Lower Cretaceous Sarcheshmeh Formation in two stratigraphic sections from the western Koppeh-Dagh Basin (NE Iran). The analyzed successions consist mainly of deep-marine shales and marlstones with intercalations of thin-bedded orbitolinid-bearing limestones. These strata yielded common to abundant and fairly well to well-preserved calcareous nannofossil assemblages. The aforementioned assemblages led to the identification of parts of the NC6 and NC7a biozones that can be correlated with parts of the CC7a and CC7b zones. The organic-walled dinoflagellate cysts also show well-preserved and relatively diverse assemblages in the studied sections that corresponded to the Odontochitina operculata Zone. Additionally, within the limestone intercalations of the Sarcheshmeh Formation, two index species of orbitolinids, Praeorbitolina cormyi Schroeder, and P. wienandsi Schroeder, were recorded, indicating the presence of the Praeorbitolina cormyi Zone. According to the literature, these recognized biozones are compatible with the ammonite zones of Deshayesites forbesi, Deshayesites deshayesi, and Dufrenoyia furcata, as well as the Globigerinelloides blowi and Leupoldina cabri planktonic foraminiferal zones. By the compilation of all acquired data, an age of late early Aptian (late Bedoulian) is deduced for the Sarcheshmeh Formation in the studied sections.

Assessing environmental change associated with early Eocene hyperthermals in the Atlantic Coastal Plain, USA

Abstract. Eocene transient global warming events (hyperthermals) can provide insight into a future warmer world. While much research has focused on the Paleocene–Eocene Thermal Maximum (PETM), hyperthermals of a smaller magnitude can be used to characterize climatic responses over different magnitudes of forcing. This study identifies two events, namely the Eocene Thermal Maximum 2 (ETM2 and H2), in shallow marine sediments of the Eocene-aged Salisbury Embayment of Maryland, based on magnetostratigraphy, calcareous nannofossil, and dinocyst biostratigraphy, as well as the recognition of negative stable carbon isotope excursions (CIEs) in biogenic calcite. We assess local environmental change in the Salisbury Embayment, utilizing clay mineralogy, marine palynology, δ18O of biogenic calcite, and biomarker paleothermometry (TEX86). Paleotemperature proxies show broad agreement between surface water and bottom water temperature changes. However, the timing of the warming does not correspond to the CIE of the ETM2 as expected from other records, and the highest values are observed during H2, suggesting factors in addition to pCO2 forcing have influenced temperature changes in the region. The ETM2 interval exhibits a shift in clay mineralogy from smectite-dominated facies to illite-rich facies, suggesting hydroclimatic changes but with a rather dampened weathering response relative to that of the PETM in the same region. Organic walled dinoflagellate cyst assemblages show large fluctuations throughout the studied section, none of which seem systematically related to CIE warming. These observations are contrary to the typical tight correspondence between climate change and assemblages across the PETM, regionally and globally, and ETM2 in the Arctic Ocean. The data do indicate very warm and (seasonally) stratified conditions, likely salinity-driven, across H2. The absence of evidence for strong perturbations in local hydrology and nutrient supply during ETM2 and H2, compared to the PETM, is consistent with the less extreme forcing and the warmer pre-event baseline, as well as the non-linear response in hydroclimates to greenhouse forcing.

Dinocyst assemblages and water surface conditions in the Sea of Marmara during MIS 6 and 5 from two long cores

The Sea of Marmara is the connection between the vast Black Sea-Caspian Sea basin (Pontocaspian) and the Global Ocean via the Mediterranean Sea. Its water levels and water conditions has widely varied over times. Combining two cores in the Sea of Marmara (Turkey) and using organic-walled dinoflagellate cyst assemblages as the main proxy (combined with alkenones, diatoms and benthic foraminifera), allow qualitatively reconstructing water conditions during Marine Isotopic Stage (MIS) 6 and 5, such as salinity and oxygen level. A clear main marine phase is illustrated in MIS 5e. A minor marine incursion occurred during MIS 5c, mostly supported by alkenone data. The rest of the record indicates brackish Pontocaspian conditions, with more Spiniferites inaequalis in MIS 6 and more S. cruciformis in the non-marine parts of MIS 5.At the MIS 6/MIS 5 transition, an earlier initial marine flooding in the Sea of Marmara (dinocyst assemblages) in comparison to the Black Sea was highlighted. The marine reconnection occurred at different moments as seen in the terrestrial vegetation reconstructed from pollen analysis linking the two seas.The sapropels of the Sea of Marmara form when marine water penetrates at depth from the Aegean Sea beneath a layer of lower salinity water. Variations of the residence time of the marine deep water in a stratified Sea of Marmara are potentially an important factor underlying hypoxia/anoxia and sapropel deposition. When combining surface water proxies with benthic foraminifera (test assemblages and presence of inner organic lining), it appears that the successive MIS 5 sapropels formed under decreasing salinity and oxygen availability conditions as the marine inflow was increasingly restricted. Understanding the hydrologic evolution of the Sea of Marmara during MIS 5 implies taking into account that the Bosphorus and Dardanelle straits are hydrodynamically coupled and may not be simplified as on/off switches based on present day sill depths.

Upper Valanginian – Hauterivian calcareous dinoflagellate cyst and calpionellid zones from the Agrio Formation (Neuquén Basin), Argentina

In this paper we describe for the first time the calcareous dinoflagellate cysts and calpionellid associations from upper Valanginian-Hauterivian orbital-driven rhythmic distal ramp deposits (Agrio Formation) in the Neuquén Basin, Argentina. Three stratigraphic sections from the northern Neuquén province (Loma La Torre and El Portón) and southern Mendoza province (Arroyo Loncoche) were studied. The Agrio Formation contains a low diverse association of these microfossils, among which ten calcareous dinoflagellate cyst species and five calpionellid species were recognized, all of them known from the Lower Cretaceous pelagic sediments of the Tethyan region. Two calcareous dinoflagellate cyst zones (Carpistomiosphaera valanginiana, and Stomiosphaera echinata), as well one calpionellid zone (Tintinnopsella), previously proposed for the Tethyan realm, are confirmed for the Agrio Formation. These zones are consistent to late Valanginian - Hauterivian age assignments previously established based on ammonites, nannofossils, and organic-walled dinoflagellate cysts.

Organic-walled dinoflagellate cyst assemblages in surface sediments of the Ría de Vigo (Atlantic margin of NW Iberia) in relation to environmental gradients

Organic-walled dinoflagellate cyst assemblages were analysed in 31 surface sediment samples from the Ría de Vigo (NW Iberia) to obtain supporting evidence for the interpretation of past environmental signals from sediment cores. Complementarily, the totals of pollen (and pollen from riparian taxa), foraminiferal linings and freshwater algae were also considered to test their value as (palaeo)environmental indicators in fluvio-marine sedimentary environments. Abundances of foraminiferal linings gradually increased towards the ría's mouth, which supports its use as a proxy to infer the degree of marine influence in the sediment record. The ratio of dinoflagellate cysts to pollen and spores (D/P) increased with distance to the main river's mouth (and water depth) in the inner (<7 m water depth) and the outer (>20 m) parts of the ría. Total pollen concentrations decreased with water depth in the outer part but did not show any clear trend in the rest of the ría. No clear pattern in the distributions of pollen from riparian plants and spores from freshwater microalgae was detected. Multivariate analyses (clustering and RDA) performed on dinoflagellate cyst records and environmental data reflected a marked inshore-offshore distribution pattern mainly controlled by a fluvio-marine environmental gradient. Increasing abundances of cysts of Gymnodinium species and heterotrophic cysts as well as higher cyst diversity characterised the deeper environments of the outer ría. These observations suggest a relationship with increased shelf influence and are compatible with the heterotroph upwelling signal described in previous works. A very different cyst association with a predominance of autotrophic cysts characterised the intermediate and inner parts of ría, where exceptionally high cyst abundances of Lingulodinium polyedra (~56–99% and ~ 200–114,000 cysts.g−1) were reported. Abundances of cysts of L. polyedra positively correlated with shallower and higher river-influenced environments characterised by higher sea-surface temperature. Our results agree with previous studies and reinforce the value of this species as an indicator of warmer and stratified conditions. Moreover, cysts of L. polyedra positively correlated with winter sea-surface nitrate contents, which is compatible with the nutrient enrichment signal (natural or anthropogenic) that has already been described in other deep and stratified estuarine environments and is consistent with historical cyst records obtained in the Ría de Vigo. However, further research is needed to disentangle the nutrient enrichment signal from the influence of other environmental factors.

Evaluation of organic-walled dinoflagellate cyst distributions in coastal surface sediments of the China Seas in relation with hydrographic conditions for paleoceanographic reconstruction

Dinoflagellate cysts are commonly used to reconstruct past climatic, oceanographic and environmental conditions. Such applications are based on the relationship between modern dinoflagellate cysts and environmental parameters. To evaluate cyst diversity and driving factors of their distributions in sediments of the China Seas for paleoenvironmental reconstruction, dinoflagellate cysts were analyzed in 18 surface sediment samples from the Bohai Sea, the Yellow Sea (YS), the East China Sea and the South China Sea (SCS). A total of 59 dinoflagellate cyst taxa were identified with the greatest diversity reported in the SCS. The highest cyst concentrations were found in the YS, where Spiniferites ramosus, Spiniferites spp., and cysts of Pentapharsodinium spp. were predominant in the assemblages. The Yellow Sea Warm Current is likely creating the environment favoring the high numbers of Spiniferites ramosus in the YS. The highest Brigantedinium spp. abundances were observed near the Pearl River mouth and are positively correlated with sea-surface temperature (SST) in August. Spiniferites hyperacanthus, S. mirabilis, S. pacificus, and Lingulodinium spp. were most abundant in the SCS. In contrast, cysts of Protoperidinium spp. and Selenopemphix quanta were more common at the sites directly influenced by coastal upwelling rather than by river discharges. Redundancy Analysis results show that SST, sea-surface salinity (SSS) and nitrate concentration in water are statistically significant parameters influencing the cyst assemblages. We also examined the morphological characteristics of Spiniferites ramosus and recommend grouping S. bulloideus with S. ramosus in the northwest Pacific dataset for quantitatively reconstructing paleoceanographic conditions.

A new record of the Cenomanian–Turonian transgression preserved in the Ikorfat Fault zone, Nuussuaq Basin, West Greenland

The Ikorfat Fault zone in northern Nuussuaq, Central West Greenland, has preserved a hitherto unknown succession of Upper Cretaceous marine mudstones referred to the Itilli Formation, which have been removed by erosion on the footwall block and buried deeply below sea-level on the hanging-wall block. Outcrops in the fault zone provide new data on sedimentology, palynology, stable carbon isotopes and organic geochemistry in the lower part of the Itilli Formation. A new, detailed geological map of the study area is based on digital photogrammetry. Marine organic walled dinoflagellate cysts (dinocysts) demonstrate a late Cenomanian–Turonian age, coeval with the Oceanic Anoxic Event 2 (OAE2) and link the Nuussuaq Basin with the Baffin Bay and Sverdrup Basins. The Ikorfat Fault zone is part of the eastern boundary fault of the Nuussuaq Basin. Lower Campanian listric faults truncate the footwall block. Subsidence along steep normal faults continued during deposition of the volcanic Vaigat Formation of Danian to Selandian age. The downthrow of the hanging-wall block was around 2.5 km.

Late Quaternary Ponto-Caspian dinoflagellate cyst assemblages from the Gulf of Corinth, Central Greece (eastern Mediterranean Sea)

We present here the first long Quaternary record of organic-walled dinoflagellate cyst assemblages analysed from sediment cores retrieved during the International Ocean Discovery Program (IODP) Expedition 381 in the Gulf of Corinth. Site M0078A is located in the central part of the Gulf of Corinth (GoC), a semi-isolated marine basin that was repeatedly isolated and reconnected to the Mediterranean Sea during the Quaternary glacial/interglacial cycles. Our results show that dinoflagellate cysts are sorted in two major ecogroups, each group alternating between marine and isolated/brackish conditions. The marine intervals are characterised by high dinocyst diversity whereas the isolated intervals are dominated by taxa thriving in low-salinity conditions such as Spiniferites cruciformis and Pyxidinopsis psilata. In several of these assemblages, S. cruciformis is so prevalent that it forms almost monospecific assemblages. The low salinity dinocyst assemblages are reported for the first time outside the Ponto-Caspian region and they show a close affinity to modern assemblages from the Black Sea, Caspian Sea and Marmara Sea. The alternations between marine and brackish conditions recorded in the Gulf of Corinth reflect changes in surface water salinity (SSS) and temperature (SST), in response to the Quaternary glacial-interglacial cycles. These seem to be in good agreement with regional and global marine isotope and sea-level records. Our findings suggest that the study region sensitively responds to climate forcing at orbital time scales and that local factors most likely drive shifts in dinoflagellate species composition and diversity.

Coniacian–Campanian palynology, carbon isotopes and clay mineralogy of the Poigny borehole (Paris Basin) and its correlation in NW Europe

The Poigny borehole near Provins (Seine-et-Marne) provides the most complete single pristine section through the Upper Cretaceous Chalk of the Paris Basin. A well preserved and diverse palynoflora including 236 species and subspecies of organic-walled dinoflagellate cysts (dinocysts) is documented from the borehole, together with a high-resolution carbon-isotope curve (δ 13 C carb ) for the Coniacian–Campanian interval. Integration of the palynological and δ 13 C carb data provides a basis for a chemostratigraphic and biostratigraphic correlation to England and Germany. Carbon isotope events (CIEs) are used to refine the placement of sub-stage boundaries in the core, and to calibrate and correlate distinctive palynological events with those from other European sections. Thirty-three palynological events in the upper Coniacian–Campanian, judged to be of biostratigraphic significance, are described. Palynological assemblages, the peridinioid/gonyaulacoid (P/G) dinocyst ratio and clay mineralogy are compared to depositional sequences and implicate sea-level as a major driver of palaeoenvironmental change.

Carbon isotopes, palynology and stratigraphy of the Santonian–Campanian boundary: The GSSP auxiliary sections, Seaford Head (England) and Bocieniec (Poland), and correlation between the Boreal and Tethyan realms

The stratigraphy and palynology of the upper Santonian–lower Campanian (Uintacrinus socialis–Gonioteuthis quadrata zones) Newhaven Chalk are described for the Campanian auxiliary GSSP section at Seaford Head, England. A new high-resolution bulk-sediment carbonate carbon stable-isotope (δ13Ccarb) curve provides the basis to refine the carbon-isotope event (CIE) stratigraphy of the section. Results are compared to a complementary palynological study of a second Campanian auxiliary GSSP section (U. socialis–O. pilula zones) at Bocieniec, Poland. Palynological assemblages are dominated by organic-walled dinoflagellate cysts (dinocysts; 208 taxa) at both sites. A stratigraphic framework is established via review of published lithostratigraphic, macrofossil, foraminifera and calcareous nannofossil records from the study sites. Carbon isotope curves with 13 major named CIEs provide a basis for correlation of Seaford Head and Bocieniec to sections at: Trunch, England; Poigny, France; Lägerdorf, Germany; and the Campanian GSSP at Gubbio, Italy. Correlations are constrained by biostratigraphic records, including dinocyst events. The Late Santonian δ13C Event (LSE, previously termed the Santonian–Campanian Boundary Event, SCBE) provides a key correlation level between Boreal and Tethyan sections and enables the placement of base Campanian markers: extinction levels of the crinoid Marsupites and the planktonic foraminifera Dicarinella asymetrica; the first appearance of the calcareous nannofossil Aspidolithus parcus parcus; and the C34n/C33r magnetozone boundary (the primary Campanian marker), in both Boreal and Tethyan sections. A holostratigraphy for the Santonian–Campanian boundary interval that integrates CIEs, macrofossils, benthic and planktonic foraminifera, calcareous nannofossils, dinocysts and magnetostratigraphy is presented. Rhynchodiniopsis juneae sp. nov. is described.

Diatom and dinoflagellate cyst distribution in surface sediments of the Chukchi Sea in relation to the upper water masses

Diatom and dinoflagellate cyst analyses were performed on 22 surface sediment samples from the Chukchi Sea to document their geographical distributions in one of the most understudied sections of the Arctic Ocean and to examine the influence of upper water masses on these two major groups of phytoplankton. Total concentrations vary from 0.9 to 5.9 × 106 valves g−1 for diatoms and from 0.8 to 12.5 × 103 cysts g−1 for organic-walled dinoflagellate cysts, with the highest values for both groups observed in the southern part of the Chukchi Sea and away from the Bering Strait. Well-preserved microfossils were recovered, with a total of 35 and 88 taxa of dinoflagellate cysts and diatoms, respectively. The most abundant diatoms are Paralia sulcata, Thalassiosira antarctica, Thalassiosira nordenskioeldii, and resting spores of Chaetoceros spp., whereas cysts of phototrophic Alexandrium spp., Operculodinium centrocarpum sensu Wall and Dale (1966), and heterotrophic Islandinium minutum and Brigantedinium spp. were most common in the dinoflagellate cyst assemblages. Cysts of HAB-causing Alexandrium spp. were found in most of the samples, with the highest abundances in Herald Canyon where they contribute ∼56.6% to the cyst assemblage. As expected, cysts produced by heterotrophic dinoflagellates were more abundant where sedimentary diatom concentrations were the highest.Statistical analysis identified three major diatom and dinoflagellate cyst clusters: 1. Sites influenced by the Alaska Coastal Current in the eastern part of the Chukchi Sea are characterized by high abundances of P. sulcata and O. centrocarpum sensu Wall and Dale (1966); 2. The western part and Herald Canyon in the northern part of the Chukchi Sea are distinguished by diatoms Chaetoceros spp., T. antarctica and dinoflagellate cysts of Alexandrium spp. and affected by the Siberian Coastal Current and Bering Shelf Water; and 3. Assemblages in the southern part of the Chukchi Sea are recognized by noticeable abundances of T. nordenskioeldii and cryophilic diatom taxa, and dinoflagellate cysts I. minutum, as well as by overall lower percentages of cysts of Pentapharsodinium dalei and Brigantedinium spp. This work revealed the potential applicability of the combined use of diatoms and dinoflagellate cysts for reconstructions of past dynamic water mass changes in the Chukchi Sea.