Abstract Rice is the staple food of Indonesian society which continues to increase in demand due to population growth. Currently, there are 3 agricultural systems in rice cultivation, namely organic, semi-organic, and conventional. The three systems provide changes in soil physical, chemical, and biological properties, but there is not much information related to chlorophyll levels in plants. This study aims to assess chlorophyll levels and their relationship with the yield of two rice varieties in various farming systems, especially the IR64 and Mentik Wangi varieties. This research is a field survey study followed by laboratory analysis. Determination of sampling locations by purposive sampling on organic, semi-organic, and conventional rice fields with two varieties of rice. Observation parameters include chlorophyll content, harvest dry grain weight, and milled dry grain weight. The results showed that the levels of chlorophyll A in IR64 and Chlorophyll B in IR 64 and Mentik Wangi varieties with organic farming systems were higher than in semi-organic and conventional farming systems. There was a positive correlation between chlorophyll levels and the yield of two rice varieties in different farming systems.