he current research work was designed to investigate the mediating role of self-efficacy between organizational culture and occupational stress at the level of secondary school. The current research work was carried out in three districts of Punjab, i.e., District Attock, District Chakwal, and District Rawalpindi. All male secondary school teachers (SSTs) from both the public and private sectors comprised the population of this research work. A total of seven hundred and sixty-eight (768) teachers were selected (384 SSTs from the private sector and 384 SSTs from the public sector) as sample size from both sectors. The sampling technique was simply random for the collection of data. Adapted research instruments were utilized for the collection of data. One null hypothesis was framed. Data analysis was done using Statistical Package for Social Sciences (SPSS) version 25.0, Analysis of Moment Structures (AMOS) version 21.0, and MS Excel. Mean Score, Standard Deviation (SD), Factor Analysis, Confirmatory Factor Analysis (CFA), Second order CFA, Structural Equation Modeling (SEM), and Chi-square were used. The null hypothesis was rejected. It was concluded that Self-efficacy (SE) was a mediating variable between organizational culture (OC) and occupational stress (OS). Both partial and full mediation were found significant. It was recommended that continuing professional development programs for teachers of government in general and private in particular be designed to enhance teachers’ level of self-efficacy.