Oral Glucose Tolerance Test Method Research Articles

Efek Penurunan Gula Darah Ekstrak Etanol Daunkaramunting (Rhodomyrtus Tomentosa (Ait.) Hassk.) Metode Toleransi Glukosa

Research on "Test the blood sugar lowering effect of the ethanol extract of karamunting leaves (Rhodomyrtus tomentosa (ait.) Hassk.) Using the glucose tolerance method on male white mice" was carried out orally by measuring the blood sugar levels of mice every 30 minutes for 2 hours with doses of 3, 2mg, 1.6mg, and 0.8mg. The results of the study using the oral glucose tolerance test method showed that the effect of glucose tolerance from karamunting leaf extract at a dose of 3.2mg/20gBB averaged blood sugar levels after administration of 50% glucose from 294 mg/dl to 123mg/dl, a dose of 1.6mg/dl. 20gBB average blood sugar level after administration of glucose 50% from 269.33 mg/dl to 133.67 mg/dl, 0.8mg/20gBB average blood sugar level after administration of glucose 50% from 261.67mg/dl to 135mg/dl. From the data analysis carried out, it was found that the data were normally distributed and there was a homogeneous variance with a significant level = 0.05 and continued with a one-way anava test where ????ℎi????????????g 3.63 ≤ ???????????????????????? 4.07 which means there is no significant difference in the extract group karamunting leaf ethanol (Rhodomyrtus tomentosa (Ait.) Hassk.) It can be concluded that karamunting leaf ethanol extract is efficacious as an antidiabetic.

Efek Penurunan Gula Darah Ekstrak Etanol Daunkaramunting (Rhodomyrtus Tomentosa (Ait.) Hassk.) Metode Toleransi Glukosa

Research on "Test the blood sugar lowering effect of the ethanol extract of karamunting leaves (Rhodomyrtus tomentosa (ait.) Hassk.) Using the glucose tolerance method on male white mice" was carried out orally by measuring the blood sugar levels of mice every 30 minutes for 2 hours with doses of 3, 2mg, 1.6mg, and 0.8mg. The results of the study using the oral glucose tolerance test method showed that the effect of glucose tolerance from karamunting leaf extract at a dose of 3.2mg/20gBB averaged blood sugar levels after administration of 50% glucose from 294 mg/dl to 123mg/dl, a dose of 1.6mg/dl. 20gBB average blood sugar level after administration of glucose 50% from 269.33 mg/dl to 133.67 mg/dl, 0.8mg/20gBB average blood sugar level after administration of glucose 50% from 261.67mg/dl to 135mg/dl. From the data analysis carried out, it was found that the data were normally distributed and there was a homogeneous variance with a significant level = 0.05 and continued with a one-way anava test where Fhitung 3.63 ≤ Ftabel 4.07 which means there is no significant difference in the extract group karamunting leaf ethanol (Rhodomyrtus tomentosa (Ait.) Hassk.) It can be concluded that karamunting leaf ethanol extract is efficacious as an antidiabetic.

The Test Effect of Glucose Tolerance of Green Apple (Mallus sylvestris Mill.) Juice on Male White Mice

Diabetes Mellitus or diabetes is a disease characterized by an increase in blood sugar levels beyond normal limits due to disruption of carbohydrate metabolism due to a deficiency of the insulin hormone caused by impaired pancreatic function. Glucose tolerance is disturbed when a person's blood sugar level on the glucose tolerance test is above normal but not high enough. Green Apple (Mallus sylvestris Mill.) Glucose Tolerance Test. Against White Male Mice orally by measuring mice's blood sugar levels every 30 minutes for 3 hours at a dose of 1.12 g; 2.24 g; and 4.48 g. The study's results using the oral glucose tolerance test method showed that the glucose tolerance effect of green apple juice at a dose of 1.12 g averaged blood sugar levels from 160.33 mg/dl to 89.33 mg/dl, at a dose of 2.24 g. – average blood sugar level from 204.67 mg/dl to 122.33 mg/dl and a dose of 4.48 g average blood sugar level from 329.67 mg/dl to 175.00 mg/dl. At a dose of 4.48 g, it can provide a faster effect on lowering the glucose levels of the test animals from statistical testing utilizing the T-test obtained tcount ≤ ttable means that there is no significant difference between the test substance group and the normal group.

GLP-1 Targeted Novel 3-phenyl-7-hydroxy Substituted Coumarins Mitigate STZ-induced Pancreatic Damage and Improve Glucose Homeostasis in OGTT Method.

Worldwide, type 2 diabetes mellitus accounts for a considerable burden of disease, with an estimated global cost of >800 billion USD annually. For this reason, the search for more effective and efficient therapeutic anti-diabetic agents is continuing. Recent studies support the search for coumarins or related compounds with potential blood glucose-lowering properties. The study aims to design, synthesize and evaluate the hypoglycemic activity of a new class of 7-hydroxy coumarin derivatives. To explore and establish the in-silico-driven pharmacological role of a new class of 7- hydroxy coumarin derivatives as the therapeutic strategies against type 2 diabetes mellitus. A new class of 7-hydroxy coumarin derivatives was designed by assessment of their physicochemical properties and molecular docking against the Glucagon-like peptide-1 (GLP-1) receptor. Two novel series of 30 compounds were synthesized. The chemical structures of all the synthesized analogues have been elucidated by spectral studies of IR, 1H-NMR, and mass spectroscopy. After considering the molecular docking score and their physicochemical properties, the compounds were screened out for the evaluation of their hypoglycemic potential. The compounds were investigated for their hypoglycemic activity using a streptozotocin (STZ) induced diabetic model and an oral glucose tolerance test (OGTT) method at different dose levels. The molecular docking studies of synthesized derivatives reveal significant molecular interaction with the various amino acid residues of the GLP-1 receptor. IR spectral analysis revealed a strong band of -NH stretching in the range of 3406.7-3201.61 cm-1 and one strong band for the lactone carbonyl group of the coumarin ring in the range of 1722.0-1703.5 cm-1, confirming the chemical structure of all produced compounds. The synthesized coumarin analogues with the best docking score exhibited remarkable hypoglycemic potential as assessed by the STZ model and the OGTT method. Coumarin derivatives explored a good structure-activity relationship (SAR) and produced significant hypoglycemic potential.

A test to measure oral iron absorption and glucose tolerance simultaneously in 18 to 55 year old premenopausal women

Several methods are available to measure iron absorption (IA). The oral iron absorption test (OIAT) measures IA based on a change in serum iron (ΔSeFe) concentration after an oral iron dose. The objective of this study was to validate the OIAT by comparing it to the reference method of fractional iron absorption (FIA) using red blood cell incorporation of stable iron isotopes from a labeled iron dose. A second objective was to assess whether the OIAT could be done simultaneously with an oral glucose tolerance test (OGTT), since iron deficiency and glucose intolerance may coexist, especially among overweight individuals with low-grade inflammation. In this prospective experimental study, 116 women were enrolled and IA was measured using two different approaches 1) FIA from a labeled test meal containing 6mg of 57Fe and 2) the OIAT assessing ΔSeFe at 2h after the intake of 100mg oral iron, done simultaneously with an OGTT. Markers of iron status, glycaemia and inflammation, and serum hepcidin, were measured. Prevalence of anemia and iron deficiency (defined as low serum ferritin) were 21% and 14%, respectively. ΔSeFe during the OIAT-OGTT was positively associated with FIA (r=0.578, p<0.001). ΔSeFe was not significantly correlated with markers of glucose and insulin metabolism during the OIAT-OGTT. The combined OIAT and OGTT method described here correlates well with FIA measured by stable iron isotopes, and could provide information on both IA and glucose tolerance in a single 2-h test, decreasing the burden on patients. clinicaltrials.gov (NCT03642223).

Effects of Packed Red Cell Transfusion on Blood Glucose Concentrations in Beta Thalassemia Major (BTM) Ashort Presentations of Personal Experience

The most accurate method with which to evaluate altered glucose metabolism in patients with TM is still controversial. Even if the annual oral glucose tolerance test (OGTT) by the age of 10 years is the recommended method, a diagnosis of ’normal’ glucose tolerance during OGTT does not exclude abnormal postprandial glucose levels at home . There is now evidence that the OGTT method, evaluating fasting and 2-h post load glucose, may miss episodes of hyperglycaemia . Furthermore, the credibility of Hb A1c has been questioned because the hemoglobin composition of patients’ erythrocytes are considerably modified, due to regular and frequent transfusions. The results may be falsely increased or decreased depending on the proximity to transfusion, shortened erythrocyte lifespan and the assay used . It has been demonstrated recently that the continuous glucose monitoring system (CGMS) is a useful and valid tool in defining glucose metabolism in children and adults affected by TM with early glucose derangements . Indeed, the CGMS allows monitoring of glycaemic profiles throughout a period of 72 h for a total of 288 glycaemic registrations per day. It identifies glycaemic excursions and constitutes a valid device to understand the 24-h glycaemic trend and profiles. Rimondiet al. investigated the value of using CGMS in six TM patients with abnormal glucose homeostasis after an oral glucose tolerance test (OGTT) . Two-hour OGTT glucose values and CGMS fluctuations were classified as normal if < 7.8 mmol/l, impaired if 7.8 to 11.1 mmol/l, diabetic if > 11.1 mmol/l. The TM patients spent from 1 to 23% of the time with a blood glucose level from 7.8 to 11.1 mmol/l. we evaluate three patients with Beta Thalassemai major using CGMSPatient 1 A 15 year old male with TM presented with nocturia. His FBG was 5.6 mmol/L and OGTT showed a BG level at 2hrs of 8.5 mmol/l. His CGMS showed a diabetic range of BG after dinner and overnight. Based on this tracing, a basal insulin (Glargine) was prescribed at night. A satisfactory response was recorded by CGMS .Patient 2 A 14 year old girl with TM with no symptoms related to glycemic abnormalities. Her FBG was 4.9 mmol/L and an OGTT showed a BG level at 2 hrs of 6.9 mmol/L (IGT). CGMS tracing showed prolonged persistent hyperglycemia after lunch suggesting a need for prandial insulin to cover her carbohydrate load. Insulin aspart before lunch properly controlled her glycemia . Patient 3 A 13 year old boy with TM had a normal FBG (4.5 mmol/L) and OGTT (2h BG =7.6 mmo/l). Applying CGMS monitoring showed IGT state. Furthermore, the effect of packed red cell transfusion showed marked reduction of his BG before meals and overnight. This is the first case to document the rapid beneficial effect of blood transfusion on glycemia in patiens with Beta thalssemia Major Advances in TM care have led to improved survival and quality of life in patients with TM; however, many chronic endocrine diseases have emerged as a result of potential endocrine complications by routine screening and a high index of suspicion is imperative for patients with TM to receive timely treatment.Our results demonstrate that the CGMS is a useful method to detect the variability of glucose fluctuations and offers the opportunity for better assessment of glucose homeostasis in TM patients. Proper and early iron chelation or the use of intensive iron chelation in those with high iron load the new oral chelators have been shown to decrease or reversethese glycemic abnormalities.In addition, optimization of insulin therapy with the help of CGMS appears to be clinically useful and costeffective approach. DisclosuresNo relevant conflicts of interest to declare.

UJI EFEK ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta, Merr) PADA TIKUS PUTIH JANTAN GALUR WISTAR

Kaca piring leaf ( Gardenia augusta Merr.) was often used in traditional drugs for treatment diabetes mellitus. This research aim to know the antihiperglycemic effect ethanolic extract of kacapiring leaf on white rats male strain of Wistar and how much antihiperglycemic effect compared with glibenklamid. This study used oral glucose tolerance test method with loading glucose 4,5 g/kgW. Tested animal where 24 white male rats strain of Wistar age 2-3 months with weight 180-250 gram, devided into 4 groups, each groups consist of 6 rats. Group I was as negative control group given CMC-Na 1%, group II was as positive control group given glibenklamid dose 1,35 mg/kgBB, group III and IV where given ethanolic extract of kacapiring leaf dose 500 mg/kgBB and 250 mg/kgBB. Glibenklamid and exstract are given orally 60 minutes before glucose. Blood was taken from orbitalis sinus at minute (-90), (-60), 0, 30, 60, 120, 180, 240, and 300. Blood glucose level was determined with GOD PAP enzymatic method, absorbance was observed using spectrophotometer at 500 nm. The result of the study was performed by giving ethanolic extract of kacapiring leaf dose 500 mg/kgW and 250 mg/kgW had antihiperglycemic effect. Ethanolic extract of kacapiring leaf dose500 mg/kgW and 250 mg/kgW could reduce blood glucoce level 58,97% and 80,60% compared with glibenklamid dose 1,35 mg/kgW could reduce blood glucoce level 73,93%.