Optimum Culture Conditions For Production Research Articles

Laccase production and dye decolorization by Trametes versicolor: Application of Taguchi and Box-Behnken methodologies

Objective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experiments were designed with five factors (glucose, yeast extract, CuSO 4 , inoculum size and pH) at three levels with orthogonal array layout of L27 (3 5 ). Then, the optimum conditions for high decolorization activity of Reactive Blue 49 by obtained crude laccase were also investigated using Box-Behnken methodology. Results: The optimum culture conditions for production of high amounts of laccase were detected as 2 g L -1 of glucose, 5 g L -1

Optimum culture conditions for production of exfoliative toxin by Staphylococcus hyicus.

Optimum culture conditions for the production of exfoliative toxin by Staphylococcus hyicus (shET) were examined. High shET activity was obtained from the culture filtrate of HI and TY broth inoculated with S. hyicus. The pH in these two media ranged from 7 to 8.5 during bacterial culture, while the lowest pH in TS and BHI broth was less than 6. shET activity in the culture filtrate from TY broth inoculated with 10(7) CFU of S. hyicus per ml was higher than that in TY broth inoculated with 10(6) and 10(8) CFU of bacteria per ml. When shET activity in the culture filtrate was measured under various shaking conditions, the culture filtrate shaken at 75 oscillations per min had the highest shET activity of the five shaking conditions. shET activity of the culture filtrate of TY broth to which protease inhibitor had been added was the same as that of TY broth without inhibitor. shET activity in a shaking culture in an Erlenmeyer flask was also the same as that in sac culture and that in shaking culture using a shaking (Sakaguchi) flask. shET activity in TY broth supplemented with 100 mM glucose was significantly lower than that in TY broth without glucose. Based on the above results, the optimum culture conditions for the production of shET were as follows: inoculation of 3 x 10(9) CFU of S. hyicus strain P-1 into 300 ml of TY broth in a 2,000-ml Erlenmeyer flask, and incubation at 37 C with shaking at 75 oscillations per min.(ABSTRACT TRUNCATED AT 250 WORDS)

Optimum culture conditions for production of kefiran by Lactobacillus sp. KPB-167B isolated from kefir grains

Abstract Optimum culture conditions for the effective production of kefiran, a water-soluble polysaccharide, by Lactobacillus sp. KPB-167B isolated from kefir grains were examined. Kefiran could be produced under conditions where cell growth was observed. The pH controlled at 5.0 and the temperature at 30°C were favorable for kefiran production. The highest yield of 2.04 g/ l in broth was accomplished in a modified MRSL medium contained 10% lactose, 5 mM CaCl 2 and two-fold the original nitrogen sources for 4 d. In a modified MW (milk whey) medium with additional 5% lactose and two-fold the original nitrogen sources, 0.60 g/ l of kefiran were obtained for 5 d.

Optimum culture conditions for production by Pseudomonas chlororaphis B23 of nitrile hydratase.

We sought optimum culture conditions for the production by Pseudomonas chlororaphis B23 of nitrile hydratase activity. Addition of ferric and ferrous ions and the use of methacrylamide as an inducer greatly enhanced nitrile hydratase formation. When P. chlororaphis B23 was cultivated for 26 hr at 25°C in a medium consisting of 1 g of sucrose, 0.5 g of methacrylamide, 0.2 g of l-cysteine, 0.2 g of l-glutamate (Na), 0.2g of l-proline, 50 mg of KH2PO4, 50 mg of K2HPO4, 50 mg of MgSO4·7H20, and 1 mg of FeSO4·7H20 per 100 ml of tap water with the pH controlled at pH 7.5 to 7.8, the enzyme activity in the culture broth was 900-times that previously reported.