A lack of standardization in monitoring protocols has hindered the accurate evaluation of microplastic (MP) pollution in the open sea and its potential impacts. As sampling techniques significantly influence the amounts of MPs contained in the sample, the aim of this study was to compare two sampling methods: Manta trawl (size selective approach) and grab sampling (volume selective approach). Both approaches were applied in the open sea surface waters of the North-east Atlantic Ocean. Onshore sample processing was carried out using the innovative tape lifting technique, which affords a series of advantages, including prevention of airborne contamination during analysis, without compromising integrity of the results. The results obtained indicated an MP concentration over four orders of magnitude higher using grab sampling compared to the Manta net approach (mean values equal to 0.24 and 4050 items/m3, respectively). Consequently, the sole quantification of MPs using results obtained with the Manta trawl resulted in a marked underestimation of abundance. Nevertheless, the grab sampling technique is intricately linked to a risk of collecting non-representative water volumes, consequently leading to an overestimation of MPs abundance and a significant inter-sample variability. Moreover, the latter method is unsuitable for use in sampling larger MPs or in areas with low concentrations of MP pollution. The optimal sampling method therefore is dependent on the specific objectives of the study, often resulting in a combination of size and volume selective methods. The results of this study have the potential to contribute to the standardization of monitoring protocols for microplastics, both during the sampling phase and sample processing.
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