Optimization Of Extraction Parameters Research Articles

Technology of dry extract from the <i>Filipendula ulmaria</i> (L.) Maxim. and preliminary assessment of its safety profile

Introduction. Medicines of natural origin have advantages over synthetic drugs and occupy a significant share in the assortment of pharmacies. Filipendula ulmaria (L.) Maxim., characterized by a chemical composition rich in phenolic compounds and various types of biological activity has the potential in terms of the development of new herbal medicines. In an in vivo experiment, F. ulmaria extract showed immunomodulatory, anti-inflammatory, osteogenic activity and effectively stimulated myelopoiesis in conditions of microbial damage of bone tissue.Aim. Development of an optimal technology for obtaining dry extract of F. ulmaria by remaceration and investigation of its аcute toxicity.Materials and methods. The technology of extract was developed in a laboratory reactor equipped with a water heater jacket and a stirrer. The content of flavonoids was determined by differential spectrometry. Flowability was determined on a flowability tester for powders and granules, bulk density was determined on a bulk density tester for powders and granules, moisture content was developed on a moisture content analyzer. Acute toxicity was determined on Wistar rats.Results and discussion. The dry extract of F. ulmaria, obtained using 70 % ethanol by remaceration, is effective in conditions of microbial damage of bone tissue and bone marrow, which determined the choice of extragent and the type of extract. The optimal parameters for obtaining the extract were determined (three-fold maceration at a water jacket temperature of 80 °C, the ratio of raw materials to extragent 1 : 14, time 30 min), its main technological characteristics were determined and a wet granulation method for improving them was proposed. In the study of acute toxicity of the extract, according to the results of toxicometry for 14 days after administration and necropsy data, the studied extract was classified as low-toxic substance.Conclusion. The dry extract of F. ulmaria, obtained by remaceration using 70 % ethanol, has specific activity in conditions of microbial damage to bone tissue. Optimal extraction parameters allow to increase the yield of target components, it is advisable to improve the technological parameters of the extract by wet granulation. The assessment of the safety profile of the dry extract from F. ulmaria, along with its high biological activity, provides a perspective for its further study and implementation into medical practice.

Preparation of a sustainable magnetic sorbent for the extraction and preconcentration of progestogens in natural water samples

A film composed of agarose and graphene (G) and magnetic nanoparticles (G-MNPs) is proposed as a sorbent for the extraction and determination of medroxyprogesterone (MED), levonorgestrel (LEV), norethisterone (NOR) and progesterone (PRO) in natural water samples. Both the preparation of the film and the extraction procedure were optimized. The optimal extraction parameters were as follows: isopropyl alcohol as activation solvent, sample pH value of 3.0, extraction time of 30 min, 1.00 mL of acetonitrile as eluent, elution time of 5 min and sample volume of 100.00 mL. HPLC with photodiode array detector was used for the separation and determination. The method presented a linear range between 2.50 and 75.0 μg L−1 for all analytes, and the LODs were between 1.40 and 1.80 μg L−1. The method was applied to natural water samples, obtaining satisfactory recovery values (75–111 %). In conclusion, for the immobilization of the G-MNPs, agarose was used, which is a non-toxic, renewable and biodegradable material. The G-MNPs-agarose film was reused up to 70 times, without losing its extraction capacity significantly and presenting excellent sorbent properties, which allow the extraction and preconcentration of the progestogens under study.

Isolation and Identification of Indole Alkaloids from Aspergillus amstelodami BSX001 and Optimization of Ultrasound-Assisted Extraction of Neoechinulin A

This study aimed to investigate the alkaloid secondary metabolites of Aspergillus amstelodami BSX001, a fungus isolated from Anhua dark tea, and to improve the extraction yield of the active ingredients by optimizing the extraction process. The structural characterization of the compounds was investigated using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The antioxidant activity of echinulin-related alkaloids was evaluated by determining the total reducing power and DPPH radical scavenging capacity. The extraction process of the compound with optimum activity was optimized by a single-factor test and response surface methodology (RSM) combined with Box–Behnken design (BBD). The optimized result was validated. Finally, a new alkaloid 8-hydroxyechinulin (1), and four known alkaloids, variecolorin G (2), echinulin (3), neoechinulin A (4), and eurocristatine (5), were isolated. Echinulin-related compounds 1, 3, and 4 possessed certain antioxidant activities, with IC50 values of 0.587 mg/mL, 1.628 mg/mL, and 0.219 mg/mL, respectively, against DPPH radicals. Their total reducing power at a concentration of 0.5 mg/mL was 0.29 mmol/L, 0.17 mmol/L, and 4.25 mmol/L. The extraction process of neoechinulin A was optimized with the optimum extraction parameters of 72.76% methanol volume fraction, 25 mL/g solid–liquid ratio, and 50.8 °C soaking temperature. Under these conditions, the extraction yield of neoechinulin A was up to 1.500 mg/g.

Response surface optimization for deep eutectic solvents extraction of bamboo shoots proteins

To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of Chimonobambusa purpurea Hsueh f. & T.P.Yi (syn. Chimonobambusa neopurpurea T.P.Yi) bamboo shoots. Choline chloride and proline were utilized as hydrogen bond acceptors in a total of eight deep eutectic solvents, whereas glucose, sucrose, glycerol, ethylene glycol, acetylpropionic acid, malic acid, and lactic acid were used as hydrogen bond donors. This work examined the protein extraction from bamboo shoots using deep eutectic solvents. Single-factor experiments were conducted to determine the optimal composition of the deep eutectic solvents, along with the ideal gradients of material-liquid ratio gradients, water content, extraction time, and water bath temperature. The impact and interplay of various variables on the protein extraction rate of bamboo shoots were examined using the Box-Behnken Design and Response Surface Methodology. Both the water bath temperature and the extraction time significantly influenced the protein extraction rate of bamboo shoots. Notably, the interaction between extraction time and water bath temperature had the most substantial effect on the protein extraction rate of bamboo shoots. The quadratic response surface regression equation for the protein extraction rate of bamboo shoots relative to the actual values of each factor was obtained as follows: Protein extraction rate = −193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A2 + 0.685 B2 - 0.06455 C2. Finally, this work predicted optimum extraction parameters for bamboo shoots with choline chloride-sucrose (molar ratio 1:2), a material-liquid ratio of 1:50, a water content of 74 %, an extraction time of 4 h, and a water bath temperature of 44 ℃. Under the optimal conditions, the protein extraction yield was 59.39±0.45 %, which aligned with the predicted value from the response surface fitting equation. This indicated that the optimised regression equation may have practical significance. Compared with conventional extraction using a sodium hydroxide (NaOH) solution, deep eutectic solvents extraction of bamboo shoots is more environmentally friendly, non-toxic and harmless. It provides a theoretical and experimental reference for industrial extraction and offers a reference for deep eutectic solvent development in bamboo shoot resource utilization.Key message:To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of bamboo shoots.

Ultrasound assisted hot water extraction of polysaccharides from Taraxacum mongolicum: Optimization, purification, structure characterization, and antioxidant activity.

Ultrasound assisted hot water extraction (UAHWE) was applied to extraction of polysaccharides from Taraxacum mongolicum with hot water as extract solvent. Experimental factors in UAHWE process were optimized by response surface methodology. The optimal extraction parameters to achieve the highest Taraxacum mongolicum polysaccharides (TMPs) yield (12.08±0.14)% by UAHWE were obtained under the ultrasound power of 200W, extraction temperature of 62°C, solid-to-liquid ratio of 1:20g/mL, and extraction time of 40min, and then the crude TMPs were further purified by DEAE-52 and Sephadex G-100 chromatography to obtain a homogenous polysaccharide fraction (TMPs-1-SG). Subsequently, the structure of TMPs-1-SG was characterized by UV-vis, Fourier transform infrared spectroscopy (FT-IR), high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), scanning electron microscope (SEM), transmission electron microscopy (TEM), and Congo red test. The results display that TMPs-1-SG with an average molecular weight of 5.49 × 104Da was comprised of mannose (Man), galactose (Gal), xylose (Xyl), and arabinose (Ara) with a molar ratio of 39.85:52.61:27.14:6.30. Moreover, TMPs-1-SG did not contain a triple helix structure. Furthermore, TMPs-1-SG and TEM presented a sheet-like, rod-shaped, and irregular structure. Finally, the antioxidant activity of TMPs-1-SG was evaluated by in vitro experiment. The IC50 values of scavenging DPPH and OH radicals for TMPs-1-SG achieved 0.71mg/mL and 0.75mg/mL, respectively. The findings can provide an effective method for extracting polysaccharides from natural resources.

Establishment of HPLC method and optimization of ultrasonic-assisted extraction process for two lignan active substances in Asarum heterotropoides

In the present study, developed a dependable HPLC technique to quantify asarinin and sesamin in Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. The optimal conditions for the ultrasonic-assisted extraction were determined by conducting single-factor experiment, Plackett-Burman design experiment, and Box-Behnken response surface method. Based on actual operation factors and analysis, the optimal extraction parameters were methanol volume fraction of 80% (v/v), liquid-to-material ratio of 10:1 (mL/g), ultrasonic time of 40 min, ultrasonic temperature of 51°C, and ultrasonic power of 300 W. When subjected to these conditions, the average asarinin and sesamin content were found to be 13.40 mg/g and 2.39 mg/g, respectively, with a relative error of 1.04% and 3.46%, which was consistent with the theoretical value. This study can offer a theoretical reference for the extraction of asarinin and sesamin in Asarum.

Green Approach to Enhance the Recovery of Polyphenols from Blackcurrant and Bilberry Leaves: Evaluation of Microwave-Assisted and Pressurized Liquid Extraction.

The aim of the present study was to evaluate microwave-assisted (MAE) and pressurized liquid extraction (PLE) for the recovery of polyphenols from blackcurrant and bilberry leaves and the preservation of their antioxidant activity. The extractions were carried out varying the solvent/solid (SS) ratio, temperature and time. During MAE, increasing the SS ratio increased the polyphenol concentration in the extracts from blackcurrant and bilberry leaves, while increasing the temperature had a positive effect only on bilberry polyphenols. During PLE, only a temperature increase was a determining factor for the isolation of blackcurrant leave polyphenols. Based on polyphenol recovery, optimal extraction parameters were established resulting in a yield of 62.10 and 56.06 mg/g dw in the blackcurrant and bilberry MAE extracts and 78.90 and 70.55 mg/g dw in the PLE extracts. The optimized extracts were profiled by UPLC ESI MS2, and their antioxidant capacity was evaluated through FRAP, DPPH, ABTS and ORAC assays. The characterization of the extracts by UPLC ESI MS2 confirmed flavonols as the predominant compounds in both blackcurrant and bilberry leaves, while flavan-3-ols and procyanidins were the main compounds responsible for high antioxidant capacity as confirmed by the ABTS and ORAC assays. Due to the extract composition and antioxidant capacity, PLE proved to be a technique of choice for the production of blackcurrant and bilberry leave extracts with high potential for use as value-added ingredients in the food and nutraceutical industry.

Ultrasonically intensified extraction and emulsification of Azadirachtin and its bioactivity analysis for fenugreek crop growth

Azadirachtin and neem oil, renowned for medicinal and insecticidal properties, were studied for extraction and nanoemulsion synthesis, aiming to address inefficiencies of current extraction and emulsification techniques that impede the utilization of Azadirachtin. The novel aspects include an optimized ultrasonic extraction method for azadirachtin, utilizing a binary solvent system. Additionally, the emulsification of azadirachtin neem oil and its direct application as a bioinsecticide on fenugreek crops demonstrate unique advancements in the study. Response surface methodology and Box-Behnken design optimized extraction parameters such as 35 min time, 0.4 g/mL solid-to-solvent ratio, 40% amplitude, and binary-solvent ratio of 75:25 (v/v) (ethanol:n-hexane). Ultrasound demonstrated superior efficiency with process effectiveness 22 times greater than stirred extraction. The extracted azadirachtin neem oil, superior in yield and potency, underwent concentration and nanoemulsion synthesis via ultrasonication. Stable dispersions over 30 days affirmed nanoemulsion sustainability. Emulsification parameters optimized included stabilizer (acacia gum), stabilizer ratio (2:5:100, solid:oil:water, g:mL:mL), amplitude (40%), and time (40 min). Direct application of emulsion as a bioinsecticide on seasonal fenugreek crops exhibited significant efficacy, enhancing growth and nutritional values (60–100%). Ultrasonication emerged as a superior extraction and emulsification tool, yielding potent azadirachtin neem oil-based emulsions applicable across diverse sectors, including agriculture, food, and cosmetics industries.

Optimization of Microwave Assisted Aqueous Two‐Phase Extraction of Polysaccharides from Malus hupehensis and Evaluation Its Antioxidant Activity

AbstractThe two models of microwave assisted aqueous two‐phase extraction (MATPE) of polysaccharides from Malus hupehensis are established by response surface methodology (RSM) and back propagation neural network (BP‐NN), compared their prediction ability, and comparatively optimized extraction process by using RSM and BP‐NN coupled genetic algorithm (GA), and the antioxidant activity of M. hupehensis polysaccharides (MHPs) is evaluated by the scavenging ability of free radicals. The results show that the predictive ability of BP‐NN is significantly better than that of RSM. The optimal extraction parameters to achieve the highest MHPs yield (4.71 ± 0.04)% by MATPE are obtained through BP‐NN coupled GA method under the microwave power of 337 W, mass fraction of ammonium sulfate of 23%, ethanol concentration of 29%, and liquid‐to‐solid ratio of 22 mL g−1. The IC50 values of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and hydroxyl radicals (OH) scavenging capacities for MHPs achieve 5.13 and 3.29 mg mL−1, respectively. The findings provide important references for the development and utilization of M. hupehensis.

Recovery of uranium from phosphate ore of Iran mine: Part II- solvent extraction of uranium from wet-process phosphoric acid

The current study in laboratory scale was aimed at developing an optimal two-stage extraction process for production of yellow cake from the phosphoric acid solution that was extracted from the Sheikh Habil phosphate rocks in central Iran. To attain an optimized extraction process, different mixtures of D2EHPA + TOPO + TBP were examined. A total extraction cycle including extraction, stripping and yellow cake precipitation was run to realistically evaluate the performances of the selected mixtures-conditions. As for optimization of the operational conditions, we exploited central composite design (CCD) method, that is a branch of the surface response methodology (RSM), and analyzed the results with standard statistical methods. The O/A (organic to aqueous ratio) = 2, 11 min extraction time, 5 wt% TOPO concentration, 10–15 wt% TBP concentration, 20 wt% D2EHPA concentration, 800 mv oxidation-reduction potential, and 25 °C temperature were obtained as optimum extraction parameters. The O/A = 15, 11 min stripping time, 50 wt% phosphoric acid concentration, 250 mv oxidation-reduction potential, and 40 °C temperature were obtained as optimum stripping parameters. Finally on the last uranium extraction from the H3PO4 strippant on the optimum conditions, the concentration of uranium was more than 4500 ppm with extraction efficiency near to 90 %. Scrubbing of organic phase was done by 2 wt% of sulfuric acid solution at 40 °C and precipitation of ammonium uranyl carbonate (AUC, (NH4)4UO2(CO3)3) was done with 2 M ammonium carbonate solution at an O/A ratio of 1/2 with efficiency more than 95 % in the uranium precipitation process.

ULTRASOUND-ASSISTED DEEP EUTECTIC SOLVENT EXTRACTION OF PHENOLIC COMPOUNDS FROM ROSE MYRTLE

Rose myrtle fruits contain high levels of phenolics and other bioactive compounds. To increase the extraction efficiency of phenolic compounds, ultrasound-assisted- deep eutectic solvents (DESs) was proposed. First of all, six different DESs were used in this research and compared with traditional solvents (ethanol, methanol, and water), and the most suitable solvents for extracting phenolic compounds from rose myrtle fruits was choline chloride: urea (molar ratio of 1:2). Then, the Box–Behnken design was used to find the optimal extraction conditions (ultrasonic power (X1), solid to liquid ratio (X2), and extraction time (X3)) for the achievement of high total phenolic contents (TPC) and antioxidant activity (IC50). The results showed that the optimum ultrasound assisted-DES extraction parameters to achieve the highest TPC and antioxidant activity (IC50) (85.19 mgGAE/g fresh fruit and 2.31 mg/g, respectively) were X1 of 233 W, X2 of 440 mg/ml, and X3 of 55 min. Compared with traditional maceration extraction, ultrasound-assisted-DES has a higher TPC and antioxidant activity (IC50) and shorter extraction time. Scanning electron microscopy images (SEM) revealed that the dissolution of bioactive components was enhanced from the disrupted cells after ultrasound-assisted-DES extraction. Consequently, the combination of ultrasound-assisted-DESs extraction provide an excellent extraction method for efficient extraction of bioactive components of rose myrtle fruit.

Investigation of Rosa species by an optimized LC-QTOF-MS/MS method using targeted and non-targeted screening strategies combined with multivariate chemometrics.

Plants of the Rosa genus are renowned for their pronounced and pleasant aroma and colors. The aim of this work was to develop a novel liquid chromatographic triple quadrupole time-of-flight tandem mass spectrometric (LC-QTOF-MS/MS) method for the investigation of the bioactive fingerprint of petals of different genotypes belonging to Rosa damascena and Rosa centifolia species. Central composite design (CCD) of response surface methodology (RSM) was used for the optimization of the LC-QTOF-MS/MS method. The method was validated and target, suspect, and non-target screening workflows were applied. Statistical analysis and chemometric tools were utilized to explore the metabolic fingerprint of the Rosa species. RSM revealed that the optimal extraction parameters involved mixing 11 mg of sample with 1mL of MeOH:H2 O (70:30, v/v). Target analysis confirmed the presence of 11 analytes, all of which demonstrated low limits of quantification (LOQs; as low as 0.048 ng mg-1 ) and sufficient recoveries (RE: 85%-107%). In total, 28 compounds were tentatively identified through suspect analysis. Non-target analysis enabled the generation of robust OPLS-DA and HCA models that classified the samples according to their species with 100% accuracy. A novel LC-QTOF-MS/MS method was developed and applied in the analysis of 47 R.centifolia and R.damascena flowers belonging to different genotypes.

The Effect of Ultrasound on the Extraction and Functionality of Proteins from Duckweed (Lemna minor).

The inclusion of protein in the regular human diet is important for the prevention of several chronic diseases. In the search for novel alternative protein sources, plant-based proteins are widely explored from a sustainable and ecological point of view. Duckweed (Lemna minor), also known as water lentil, is an aquatic plant with potential applications for human consumption due to its protein content and carbohydrate contents. Among all the conventional and novel protein extraction methods, the utilization of ultrasound has attracted the attention of scientists because of its effects on improving protein extraction and its functionalities. In this work, a Box-Behnken experimental design was proposed to optimize the alkaline extraction of protein from duckweed. In addition, an exploration of the effects of ultrasound on the morphological, structural, and functional properties of the extracted protein was also addressed. The optimal extraction parameters were a pH of 11.5 and an ultrasound amplitude and processing time of 60% and 20 min, respectively. These process conditions doubled the protein content extracted in comparison to the value from the initial duckweed sample. Furthermore, the application of ultrasound during the extraction of protein generated changes in the FTIR spectra, color, and structure of the duckweed protein, which resulted in improvements in its solubility, emulsifying properties, and foaming capacity.

Phenolics and flavonoids from Polygonum posumbu and comparision of flavonoid compounds content in different tissues (leaves, stems and roots)

The growing global need for antioxidative phenolics and flavonoids for maintenance of human health resulted into search of new sustainable unexplored medicinal plants used by the traditional healers for various ailments. Many synthetic based products of phenolics and flavonoids have been used, however the demand of eco-friendly, natural herbal based products are increasing. As a result, the current study aims to explore traditional potential of Polygonum posumbu related to its phenolics and flavonoids. Optimization of extraction parameters were employed which includes: solvent selection (water, ethanol, methanol, acetone and ethyl acetate), ethanol composition (40–100%), solvent to sample ratio (30–70 ml/g), temperature (50–80 °C) and time (1–5 h). Under optimal conditions, total phenolics (TPC), total flavonoids (TFC), the extract yield (EY) and antioxidant activities of leaves extract were 162.79 ± 2.28 mg GAE/g, 56.57 ± 6.22 mg QE/g 27.96 ± 0.91%, and 27.34 ± 0.98 μg/ml respectively. Seven flavonoids were quantified in different tissues with significant (p ≤ 0.05) differences found in flavonoids contents in different parts of the plant. Highest concentration of flavonoids was observed in stems: (−)-epicatechin-53.19 ± 1.13 mg/g, myricetin-15.90 ± 0.13 mg/g, quercetin-50.66 ± 0.08 mg/g, luteolin-43.10 ± 0.47 mg/g, apigenin-16.73 ± 0.43 mg/g. Leaves and roots had the highest amount of genistein (05.06 ± 0.01 mg/g) and kaempferol (11.13 ± 0.06 mg/g) respectively. From the study it had been found that Polygonum posumbu possess a very good amount of phenolics and flavonoids and this study details first ever investigation on this plant in terms of phenolics and flavonoids. Therefore, this study enhanced the importance of this bioresource in functional food or nutraceutical industries.

Comparison of extraction processes, characterization and intestinal protection activity of Bletilla striata polysaccharides

We optimized the extraction process of Bletilla striata polysaccharides using orthogonal design, Box-Behnken design (BBD), and genetic algorithm-back propagation (GA-BP), then compared and evaluated them to confirm that the combination of BBD and GA-BP neural networks was capable of increasing polysaccharide yields and antioxidant activity. The optimal extraction parameters were as follows: liquid-to-solid ratio of 15 mL/g, extraction power of 450 W, and extraction time of 34 min. Under these conditions, the polysaccharide yield and antioxidant activity were 8.29 ± 0.50 % and 26.20 ± 0.28 (mM FE/mg). Subsequently, the polysaccharide was purified to obtain purified Bletilla striata polysaccharides 1 (pBSP1) with a Mw of 255.172 kDa. Scanning electron microscope (SEM), ultraviolet-visible detector (UV), fourier transform infrared spectrometer (FTIR), high performance liquid chromatography (HPLC), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and periodate oxidation were used to analyze the structure of pBSP1. The results showed pBSP1 had a smooth surface and a rough interior, with a composition of α-D conformation glucose (18.23 %) and β-D conformation mannose (53.77 %), and an amorphous crystal structure. According to the results of thermogravimetric and rheological tests, pBSP1 exhibits good thermal stability and viscoelastic behavior. Furthermore, pBSP1 protected lipopolysaccharide (LPS)-induced GES − 1 and Caco2 cells, the results showed pBSP1(400 μg/mL) lowered TEER synthesis in Caco2 cells as well as apoptosis and reactive oxygen species (ROS) production in both cells, indicating that pBSP1 may have an intestine protective effect.

Optimizing Quality and Shelf-Life Extension of Bor-Thekera (Garcinia pedunculata) Juice: A Thermosonication Approach with Artificial Neural Network Modeling

This study investigated the quality characteristics of pasteurized and thermosonicated bor-thekera (Garcinia pedunculata) juices (TSBTJs) during storage at 4 °C for 30 days. Various parameters, including pH, titratable acidity (TA), total soluble content (TSSs), antioxidant activity (AA), total phenolic content (TPC), total flavonoid content (TFC), ascorbic acid content (AAC), cloudiness (CI) and browning indexes (BI), and microbial activity, were analyzed at regular intervals and compared with the quality parameters of fresh bor-thekera juice (FBTJ). A multi-layer artificial neural network (ANN) was employed to model and optimize the ultrasound-assisted extraction of bor-thekera juice. The impacts of storage time, treatment time, and treatment temperature on the quality attributes were also explored. The TSBTJ demonstrated the maximum retention of nutritional attributes compared with the pasteurized bor-thekera juice (PBTJ). Additionally, the TSBTJ exhibited satisfactory results for microbiological activity, while the PBTJ showed the highest level of microbial inactivation. The designed ANN exhibited low mean squared error values and high R2 values for the training, testing, validation, and overall datasets, indicating a strong relationship between the actual and predicted results. The optimal extraction parameters generated by the ANN included a treatment time of 30 min, a frequency of 44 kHz, and a temperature of 40 °C. In conclusion, thermosonicated juices, particularly the TSBTJ, demonstrated enhanced nutritional characteristics, positioning them as valuable reservoirs of bioactive components suitable for incorporation in the food and pharmaceutical industries. The study underscores the efficacy of ANN as a predictive tool for assessing bor-thekera juice extraction efficiency. Moreover, the use of thermosonication emerged as a promising alternative to traditional thermal pasteurization methods for bor-thekera juice preservation, mitigating quality deterioration while augmenting the functional attributes of the juice.

Ultrasound assisted extraction of amino acids and nucleobases from clay minerals and astrobiological samples

The study of organic molecules in meteorite and return samples allows for the understanding of the chemistry that undergoes in our Solar System. The present work aims at studying ultrasound assisted extraction technique as effective extraction method for these molecules in extraterrestrial samples and analogs. Optimal conditions were selected from the investigation of ultrasonic frequency, irradiation duration and solvent effects on amino acids, nucleobases and dipeptides extraction yields from a model clay-rich mineral matrix. Optimal ultrasound-assisted extraction parameters were frequency of 20 kHz within 20 min irradiation time and methanol/water solvent ratio of 1. We then validated this protocol on Mukundpura and Tarda meteorite fragments and compared it to the reference extraction protocol used in astrobiology and based on 24 h extraction time at 100 °C in water We obtained similar quantitative results without any racemization with both methodologies.

Ultrasonic-Assisted Extraction, Structural Characteristics, and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance.

Alpinia officinarum Hance, a well known agricultural product in the Lei Zhou peninsula, is generally rich in polysaccharides. In order to enhance the use of A. officinarum Hance polysaccharides (AOP) in functional food, AOP was extracted using an ultrasonic-assisted extraction method, and the ultrasonic extraction parameters of AOP was optimized. Furthermore, this study investigated the physicochemical and antioxidant activities of AOPs. In addition, the structural properties were preliminarily determined using Fourier-transform infrared spectroscopy (FTIR), high performance size exclusion chromatography, and a Zetasizer. Ultimately, this study explored the mechanism underlying the antioxidant activities of AOP. The results showed that the optimal ultrasonic-assisted extraction parameters were as follows: ultrasonic time, 6 min; ratio of water to material, 12 mL/g; and ultrasonic power, 380 W. Under these conditions, the maximum yield of AOPs was 5.72%, indicating that ultrasonic-assisted extraction technology is suitable for extracting AOPs due to the reduced time and water usage. Additionally, AOPs were purified using graded alcohol precipitation, resulting in three fractions (AOP30, AOP50, and AOP70). AOP30 had the lowest molecular weight of 11.07 kDa and mainly consisted of glucose (89.88%). The half inhibitory concentration (IC50) value of AOP30 and AOP70 was lower than that of AOP50 in the ability to scavenge the ABTS radical, while a reverse trend was observed in reducing ferric ions. Notably, the antioxidant activities of AOPs were highly correlated with their polydispersity index (Mw/Mn) and Zeta potential. AOP30, a negatively charged acidic polysaccharide fraction, exhibited electron donating capacities. Additionally, it displayed strong antioxidant abilities through scavenging 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals and reducing ferric ions. In conclusion, the present study suggests that AOP30 could be developed as an antioxidant ingredient for the food industry.

Yanıt yüzey yöntemi kullanılarak Ziziphus jujube Mill yapraklarından fenolik bileşenlerin ultrason destekli ekstraksiyonunun optimizasyonu

The phenolic compounds in jujube (Ziziphus jujuba Mill) leaf were extracted using ultrasound assisted extraction (UAE) for potential of antioxidant food additive in this study. The extraction factors such as methanol concentration, temperature and time were optimized using response surface methodology (RSM) to maximize of the total phenolic content (TPC), 2,2 -diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and phenolic profile in jujube leaf extracts (JE). The best possible range for methanol concentration (25-50%), duration of ultrasound (20-40 min), ultrasonic temperature (40–60 °C) were obtained using the Box-Behnken design (BBD). The optimum extraction parameters were obtained with 25% methanol concentration, 20 min duration of ultrasound and 49.89 °C ultrasonic temperature. Ellagic, caffeic, rosmarinic acid, and rutin were determined as major phenolics in JE under optimal extraction parameters. The results revealed that UAE is an effective pretreatment for extracting bioactive ingredients from JE as potential functional food additive. Furthermore, RSM is an effective method for optimizing the UAE factors.

Sustainable In Silico-Supported Ultrasonic-Assisted Extraction of Oligomeric Stilbenoids from Grapevine Roots Using Natural Deep Eutectic Solvents (NADES) and Stability Study of Potential Ready-to-Use Extracts.

Grapevine roots, as a side-stream of a vineyard, are a sustainable resource for the recovery of oligomeric stilbenoids, such as the bioactive r-viniferin. The aim of this study is to evaluate an in silico-supported method, based on the Conductor-like Screening Model for Real Solvents (COSMO-RS), for selection of environmentally friendly natural deep eutectic solvents (NADES) with regard to the extraction of grapevine roots. The most suitable NADES system for ultrasonic-assisted extraction of r-viniferin was choline chloride/1,2-propanediol. The optimal extraction parameters for r-viniferin were determined using single-factor experiments as follows: choline chloride/1,2-propanediol 1/2 mol/mol, 10 wt% H2O, biomass/NADES ratio 1/10 g/g, and 10 min extraction time. Under optimized conditions, the extraction yield of r-viniferin from grapevine roots reached 76% of the total r-viniferin content. Regarding stability, stilbenoids in choline chloride/1,2-propanediol remained stable during 128 days of storage at ambient temperature. However, fructose/lactic acid-based NADES were observed to degrade stilbenoids; therefore, the removal of the NADES will be of interest, with a suitable method implemented using Amberlite® XAD-16N resin. As green solvents, the NADES have been used as effective and environmentally friendly extractants of stilbenoid-containing extracts from grapevine roots for potential applications in the cosmetic and pharmaceutical industry or as nutraceuticals in the food industry.