Optimization Approach Research Articles

Comparing chemical transfection, electroporation, and lentiviral vector transduction to achieve optimal transfection conditions in the Vero cell line

BackgroundTransfection is an important analytical method for studying gene expression in the cellular environment. There are some barriers to efficient DNA transfection in host cells, including circumventing the plasma membrane, escaping endosomal compartmentalization, autophagy, immune sensing pathways, and translocating the nuclear envelope. Therefore, it would be very useful to introduce an optimum transfection approach to achieve a high transfection efficiency in the Vero cell line. The aim of this study was to compare various transfection techniques and introduce a highly efficient method for gene delivery in Vero cells.MethodsIn the current study, three transfection methods were used, including chemical transfection, electroporation, and lentiviral vector transduction, to obtain the optimum transfection conditions in the Vero cell line. Vero cells were cultured and transfected with chemical transfection reagents, electroporation, or HIV-1-based lentivectors under different experimental conditions. Transfection efficiency was assessed using flow cytometry and fluorescence microscopy to detect GFP-positive cells.ResultsAmong the tested methods, TurboFect™ chemical transfection exhibited the highest efficiency. Optimal transfection conditions were achieved using 1 µg DNA and 4 µL TurboFect™ in 6 × 104 Vero cells.ConclusionTurboFect™, a cationic polymer transfection reagent, demonstrated superior transfection efficiency in Vero cells compared with electroporation and lentivirus particles, and is the optimal choice for chemical transfection in the Vero cell line.

Machine learning based data governance methods for demand response databases

With the increasing level of grid intelligence and the related demand response database expanding, it is important to study a compound problem data governance method for demand response, while the traditional data governance methods have problems such as not considering data temporality and ignoring the impact of noise and duplicate data on data repair. As a result, this project will develop an anomaly data extraction and repair model based on two-way long and short memory networks, and repair the anomaly data by respective noise smoothing, missing data filling, and duplicate data cleaning. The paper also provides an adaptive moment estimation approach for optimisation to raise the model’s accuracy. The outcomes demonstrated that the study model’s precision for anomalous data extraction was 100% and its recall rate was 80%, which was a significant improvement over the previous state. In terms of anomalous data repair, the research model had the root mean square error value and lowest mean absolute percentage error value when compared with related models, at 0.0049 MPa and 1.375% respectively. Both the abnormal data extraction and repair performance of the research model are greatly improved over the related models, and have important value in the abnormal data governance of demand response databases.

Energy Efficiency Optimisation of Joint Computational Task Offloading and Resource Allocation Using Particle Swarm Optimisation Approach in Vehicular Edge Networks

With the progression of smart vehicles, i.e., connected autonomous vehicles (CAVs), and wireless technologies, there has been an increased need for substantial computational operations for tasks such as path planning, scene recognition, and vision-based object detection. Managing these intensive computational applications is concerned with significant energy consumption. Hence, for this article, a low-cost and sustainable solution using computational offloading and efficient resource allocation at edge devices within the Internet of Vehicles (IoV) framework has been utilised. To address the quality of service (QoS) among vehicles, a trade-off between energy consumption and computational time has been taken into consideration while deciding on the offloading process and resource allocation. The offloading process has been assigned at a minimum wireless resource block level to adapt to the beyond 5G (B5G) network. The novel approach of joint optimisation of computational resources and task offloading decisions uses the meta-heuristic particle swarm optimisation (PSO) algorithm and decision analysis (DA) to find the near-optimal solution. Subsequently, a comparison is made with other proposed algorithms, namely CTORA, CODO, and Heuristics, in terms of computational efficiency and latency. The performance analysis reveals that the numerical results outperform existing algorithms, demonstrating an 8% and a 5% increase in energy efficiency.

Unveiling Promising Neuroimaging Biomarkers for Schizophrenia Through Clinical and Genetic Perspectives.

Schizophrenia is a complex and serious brain disorder. Neuroscientists have become increasingly interested in using magnetic resonance-based brain imaging-derived phenotypes (IDPs) to investigate the etiology of psychiatric disorders. IDPs capture valuable clinical advantages and hold biological significance in identifying brain abnormalities. In this review, we aim to discuss current and prospective approaches to identify potential biomarkers for schizophrenia using clinical multimodal neuroimaging and imaging genetics. We first described IDPs through their phenotypic classification and neuroimaging genomics. Secondly, we discussed the applications of multimodal neuroimaging by clinical evidence in observational studies and randomized controlled trials. Thirdly, considering the genetic evidence of IDPs, we discussed how can utilize neuroimaging data as an intermediate phenotype to make association inferences by polygenic risk scores and Mendelian randomization. Finally, we discussed machine learning as an optimum approach for validating biomarkers. Together, future research efforts focused on neuroimaging biomarkers aim to enhance our understanding of schizophrenia.

Intrusion Response Systems for the 5G Networks and Beyond: A New Joint Security-vs-QoS Optimization Approach

Intrusion Response Systems for the 5G Networks and Beyond: A New Joint Security-vs-QoS Optimization Approach

Cross validation based transfer learning for cross-sectional non-linear shrinkage: A data-driven approach in portfolio optimization

Enhanced covariance estimation approaches, such as (non-)linear shrinkage, are well established in the literature. Non-linear shrinkage estimators generally minimize a certain loss function regarding statistical assumptions about the future covariance matrix. At the same time, the problem of covariance estimation is traditionally considered from a rather restrictive view since the only available data to determine the estimation parameters is given by the return history of the actual portfolio constituents. In this study, we propose a novel and purely data-driven perspective on covariance estimation. We present a non-linear shrinkage estimator that determines the estimation parameters using cross validation to be historically optimal on a disjoint dataset of assets according to the given objective, such as minimum variance or maximum risk-adjusted return. We then transfer the historically optimal estimation parameters learned on the disjoint dataset to the actual covariance estimation problem. Thereby, the sample eigenvalues are corrected in a purely data-driven way, agnostic to theoretically derived parameters. Another benefit of focusing on disjoint data is that we address the problem of limited data availability in high-dimensional estimation problems when the number of assets exceeds the history length. Our empirical evaluation, based on a total of six stock market indices and various problem dimensions, shows that our approach outperforms existing cross-sectional estimators in minimizing variance and maximizing risk-adjusted return. While our study is limited to the cross-section, the method of parameter selection using cross validation and transfer learning can also be combined with other estimators, such as time-series methods.

Human resources development: Evaluating perceptions against policy

The objective of sustaining smart, sustainable and cohesive inclusive growth, framed by the European strategy, is in line with the global commitments of the Sustainable Development Goals. Thus, an analysis of people’s attitudes towards social policy and perceptions of professional development in Poland against European development policy is crucial. The nationwide representative sample included 1,000 respondents aged 18-64. The study used principal component analysis (PCA), an approach for the multi-response optimisation. The results indicate that respondents’ evaluations are in line with the direction of EU labour policy, where their decisions correspond to the planned priorities oriented towards inclusion and cohesion. The PCA analysis showed that employee competencies and priorities for individual standing can be considered strongly correlated. Competencies were also found to be important for the platform to support systemic development. However, the items included in the analysis that are beyond the scope of the ESF+, although even correlated, are of lesser importance. The research results allow to expand knowledge on the perception of the development policy facets promoted by the EU, supplemented by themes of leadership or immigrants. The study may become a starting point for inclusive and cohesive working climate research.

Scheduling Approaches for Joint Optimization of Age and Delay in Industrial Wireless Networks

Scheduling Approaches for Joint Optimization of Age and Delay in Industrial Wireless Networks

A Proximal ADMM-Based Distributed Optimal Energy Management Approach for Smart Grid With Stochastic Wind Power

A Proximal ADMM-Based Distributed Optimal Energy Management Approach for Smart Grid With Stochastic Wind Power

Event-Triggered Constrained Optimal Control for Organic Rankine Cycle Systems via Safe Reinforcement Learning.

The organic Rankine cycle (ORC) is an effective application for converting low-grade heat sources into power and is crucial for environmentally friendly production and energy recovery. However, the inherent complexity of the mechanism, its strong and unidentified nonlinearity, and the presence of control constraints severely impair the design of its optimal controller. To solve these issues, this study provides a novel event-triggered (ET) constrained optimal control approach for the ORC systems based on a safe reinforcement learning technique to find the optimal control law. Instead of employing the usual non-quadratic integral form to solve the control-limited optimal control problems, a constraint handling strategy based on a relaxed weighted barrier function (BF) technique is proposed. By adding the BF terms to the original value function, a modified value iteration algorithm is developed to make the control input solutions that tend to violate the constraints be pushed back and maintained in their safe sets. In addition, the ET mechanism proposed in this article is critically required for the ORC systems, and it can significantly reduce the computational load. The combination of these two techniques allows the ORC systems to achieve set-point tracking control and satisfy the control restrictions. The proposed approach is conducted based on a heuristic dynamic programming framework with three neural networks (NNs) involved. The safety and convergence of the proposed approach and the stability of the closed-loop system are analyzed. Simulation results and comparisons are presented to demonstrate its effectiveness.

Supervised Feature Selection via Collaborative Neurodynamic Optimization.

As a crucial part of machine learning and pattern recognition, feature selection aims at selecting a subset of the most informative features from the set of all available features. In this article, supervised feature selection is at first formulated as a mixed-integer optimization problem with an objective function of weighted feature redundancy and relevancy subject to a cardinality constraint on the number of selected features. It is equivalently reformulated as a bound-constrained mixed-integer optimization problem by augmenting the objective function with a penalty function for realizing the cardinality constraint. With additional bilinear and linear equality constraints for realizing the integrality constraints, it is further reformulated as a bound-constrained biconvex optimization problem with two more penalty terms. Two collaborative neurodynamic optimization (CNO) approaches are proposed for solving the formulated and reformulated feature selection problems. One of the proposed CNO approaches uses a population of discrete-time recurrent neural networks (RNNs), and the other use a pair of continuous-time projection networks operating concurrently on two timescales. Experimental results on 13 benchmark datasets are elaborated to substantiate the superiority of the CNO approaches to several mainstream methods in terms of average classification accuracy with three commonly used classifiers.

A Survey of Internet of Things based Indoor Positioning Systems based on Bluetooth Low Energy Beacon

The global positioning systems (GPS) and cellular systems face inherent limitations when it comes to enclosed environments like shopping malls, hospitals, warehouses, as they have significant shadowing effects that cause the signals to be distorted. There have been many studies on different technologies and techniques aiming to find the optimum approach to be used for indoor positioning systems (IPS). Among all the technologies adapted by researchers all around the world, Bluetooth Low Energy (BLE) beacons have been a prevalent choice for many, due to its deploying flexibility, low cost, low power consumption and ability to provide very advanced services to users. It is arduous to obtain RSSI values with great accuracy because the signals can be easily intervened by many factors like humidity, temperature, physical barriers, and interference from decoherent signals. In this paper, we presented a comparative analysis of different, fairly recent IPS that are based on BLE beacons by classifying the different proposed approaches in a structured and systematic manner and traversing the issues and challenges that exist with each technique. The accuracy of the results can be enhanced by using the most suitable positioning algorithm, the orientation of the beacon setup, and optimization methods used. A preliminary result from a case study using BLE beacons with K-Nearest Neighbours (KNN) based fingerprinting approach for indoor positioning estimation shows 86.67% accuracy.

Impact of Prevascularization on Immunological Environment and Early Engraftment in Subcutaneous Islet Transplantation.

The utilization of islet-like cells derived from pluripotent stem cells may resolve the scarcity of islet transplantation donors. The subcutaneous space is a promising transplantation site because of its capacity for graft observation and removal, thereby ensuring safety. To guarantee subcutaneous islet transplantation, physicians should ensure ample blood supply. Numerous methodologies, including prevascularization, have been investigated to augment blood flow, but the optimal approach remains undetermined. From C57BL/6 mice, 500 syngeneic islets were transplanted into the prevascularized subcutaneous site of recipient mice by implanting agarose rods with basic fibroblast growth factor at 1 and 2 wk. Before transplantation, the blood glucose levels, cell infiltration, and cytokine levels at the transplant site were evaluated. Furthermore, we examined the impact of the extracellular matrix capsule on graft function and the inflammatory response. Compared with the 1-wk group, the 2-wk group exhibited improved glycemic control, indicating that longer prevascularization enhanced transplant success. Flow cytometry analysis detected immune cells, such as neutrophils and macrophages, in the extracellular matrix capsules, whereas cytometric bead array analysis indicated the release of inflammatory and proinflammatory cytokines. Treatment with antitumor necrosis factor and anti-interleukin-6R antibodies in the 1-wk group improved graft survival, similar to the 2-wk group. In early prevascularization before subcutaneous transplantation, neutrophil and macrophage accumulation prevented early engraftment owing to inflammatory cytokine production.

SMoGW-based deep CNN: Plant disease detection and classification using SMoGW-deep CNN classifier

Diagnosing plant disease is a major role to reduce adequate losses in yield production, which further leads to economic losses. The various disease control measures are accessible without a proper diagnosis of the disease which results in a waste of expenses and time. The diagnosis of disease using images leads to unsatisfactory results in the prevalent methods due to the image clarity. It is mainly caused by the worst performance of the existing pre-trained image classifiers. This issue can be controlled by the SMoGW-deep convolutional neural network (deep CNN) classifier for the accurate and precise classification of plant leaf disease. The developed method transforms the poor-quality captured images into high quality by the preprocessing technique. The preprocessed input images contain pixels on their dimension and also the value of the threshold is analyzed by the Otsu method by which the particular disease-affected region is extracted based on the image pixels. The region of interest is separated from the other parts of the input leaf image using the K-means segmentation technique. The stored features in the feature vector are fed forward to the deep CNN classifier for training and are optimized by the SMoGW optimization approach. The experiments are done and achieved an accuracy of 94.5% sensitivity of 94.525%, specificity of 94.6%, precision of 95% with 90% of training data and under K-fold training with 95% of accuracy, 95% of sensitivity, 94.1% of specificity, and 92.1% of precession is achieved for the SMoGW-optimized classifier approach that is superior to the prevalent techniques for disease classification and detection. The potential, as well as the capability of the proposed method, is experimentally demonstrated for plant leaf disease classification and identification.

Improving big data analytics data processing speed through map reduce scheduling and replica placement with HDFS using genetic optimization techniques

Big Data Analytics (BDA) is an unavoidable technique in today’s digital world for dealing with massive amounts of digital data generated by online and internet sources. It is kept in repositories for data processing via cluster nodes that are distributed throughout the wider network. Because of its magnitude and real-time creation, big data processing faces challenges with latency and throughput. Modern systems such as Hadoop and SPARK manage large amounts of data with their HDFS, Map Reduce, and In-Memory analytics approaches, but the migration cost is higher than usual. With Genetic Algorithm-based Optimization (GABO), Map Reduce Scheduling (MRS) and Data Replication have provided answers to this challenge. With multi objective solutions provided by Genetic Algorithm, resource utilization and node availability improve processing performance in large data environments. This work develops a novel creative strategy for enhancing data processing performance in big data analytics called Map Reduce Scheduling Based Non-Dominated Sorting Genetic Algorithm (MRSNSGA). The Hadoop-Map Reduce paradigm handles the placement of data in distributed blocks as a chunk and their scheduling among the cluster nodes in a wider network. Best fit solutions with high latency and low accessing time are extracted from the findings of various objective solutions. Experiments were carried out as a simulation with several inputs of varied location node data and cluster racks. Finally, the results show that the speed of data processing in big data analytics was enhanced by 30–35% over previous methodologies. Optimization approaches developed to locate the best solutions from multi-objective solutions at a rate of 24–30% among cluster nodes.

Imbalance data: The application of RUS FCM K-RBFNN Smote with XGBoost in the elderly well-being identification

Imbalanced data is a serious binary classification difficulty in forecasting the well-being of the elderly. This paper improves the Smote algorithm from the algorithm and sample dimensions to tackle the issue of imbalanced distribution of questionnaire data. The k-means Smote is combined with RBFNN as K-RBFNN Smote in the algorithm dimension and add FCM link to resample the minority set in the sample dimension as FCM K-RBFNN Smote. In order to improve the generalization of models, the RUS module is added to the algorithm. Experiments are carried out on four improved Smote technologies and two existing Smote technologies combined with XGBoost, which is superior than the other five conventional classification models. The experimental results indicate that the performance order is RUS FCM K-RBFNN Smote > K-RBFNN Smote > FCM K-RBFNN Smote > RUS K-RBFNN Smote > K-Means Smote > FCM Smote. The RUS FCM K-RBFNN method has been identified as the optimal approach for enhancing performance, resulting in a 98.58% accuracy rate. In conclusion, Smote algorithm undergoes the implementation of K-RBFNN shows greater performance and the enhancement of FCM and RUS relies on the structure of sampling.

Secure deep learning-based energy efficient routing with intrusion detection system for wireless sensor networks

In general, wireless sensor networks are used in various industries, including environmental monitoring, military applications, and queue tracking. To support vital applications, it is crucial to ensure effectiveness and security. To prolong the network lifetime, most current works either introduce energy-preserving and dynamic clustering strategies to maintain the optimal energy level or attempt to address intrusion detection to fix attacks. In addition, some strategies use routing algorithms to secure the network from one or two attacks to meet this requirement, but many fewer solutions can withstand multiple types of attacks. So, this paper proposes a secure deep learning-based energy-efficient routing (SDLEER) mechanism for WSNs that comes with an intrusion detection system for detecting attacks in the network. The proposed system overcomes the existing solutions’ drawbacks by including energy-efficient intrusion detection and prevention mechanisms in a single network. The system transfers the network’s data in an energy-aware manner and detects various kinds of network attacks in WSNs. The proposed system mainly comprises two phases, such as optimal cluster-based energy-aware routing and deep learning-based intrusion detection system. Initially, the cluster of sensor nodes is formed using the density peak k-mean clustering algorithm. After that, the proposed system applies an improved pelican optimization approach to select the cluster heads optimally. The data are transmitted to the base station via the chosen optimal cluster heads. Next, in the attack detection phase, the preprocessing operations, such as missing value imputation and normalization, are done on the gathered dataset. Next, the proposed system applies principal component analysis to reduce the dimensionality of the dataset. Finally, intrusion classification is performed by Smish activation included recurrent neural networks. The proposed system uses the NSL-KDD dataset to train and test it. The proposed one consumes a minimum energy of 49.67 mJ, achieves a better delivery rate of 99.92%, takes less lifetime of 5902 rounds, 0.057 s delay, and achieves a higher throughput of 0.99 Mbps when considering a maximum of 500 nodes in the network. Also, the proposed one achieves 99.76% accuracy for the intrusion detection. Thus, the simulation outcomes prove the superiority of the proposed SDLEER system over the existing schemes for routing and attack detection.

Machine learning and evolutionary optimization approach for solving the flexible job-shop scheduling problem

This paper proposes a method of using machine learning and an evolutionary algorithm to solve the flexible job shop problem (FJSP). Specifically, a back propagation (BP) neural network is used as the machine learning method, the most widely used genetic algorithm (GA) is employed as the optimized object to address the machine-selection sub-problem of the FJSP, and particle swarm optimization (PSO) is utilized to solve the operation-order sub-problem of the FJSP. At present, evolutionary algorithms such as the GA, PSO, ant colony algorithm, simulated annealing algorithm, and their optimization algorithms are widely used to solve the FJSP; however, none of them optimizes the initial solutions. Because each of these algorithms only focuses on solving a single FJSP, they can only use randomly generated initial solutions and cannot determine whether the initial solutions are good or bad. Based on these standard evolutionary algorithms and their optimized versions, the JSON object was introduced in this study to cluster and reconstruct FJSPs such that the machine learning strategies can be used to optimize the initial solutions. Specifically, the BP neural networks are trained so that the generalization of BP neural networks can be used to judge whether the initial solutions of the FJSPs are good or bad. This approach enables the bad solutions to be filtered out and the good solutions to be maintained as the initial solutions. Extensive experiments were performed to test the proposed algorithm. They demonstrated that it was feasible and effective. The contribution of this approach consists of reconstructing the mathematical model of the FJSP so that machine learning strategies can be introduced to optimize the algorithms for the FJSP. This approach seems to be a new direction for introducing more interesting machine learning methodologies to solve the FJSP.

Modeling Spatiotemporal Heterogeneity of Customer Preferences With Small-Scale Aggregated Data: A Spatial Panel Modeling Approach

Abstract Customer preferences are found to evolve over time and correlate with geographical locations. Studying the spatiotemporal heterogeneity of customer preferences is crucial to engineering design as it provides a dynamic perspective for understanding the trend of customer preferences. However, existing choice models for demand modeling do not take the spatiotemporal heterogeneity of customer preferences into consideration. Learning-based spatiotemporal data modeling methods usually require large-scale datasets for model training, which are not applicable to small aggregated data, such as the sale records of a product in several regions and years. To fill this research gap, we propose a spatial panel modeling approach to investigate the spatiotemporal heterogeneity of customer preferences. Product and regional attributes varying in time are included as model inputs to support demand forecasting in engineering design. With case studies using the dataset of small SUVs and compact sedans in China's automotive market, we demonstrate that the spatial panel modeling approach outperforms other statistical spatiotemporal data models and non-parametric regression methods in goodness of fit and prediction accuracy. We also illustrate a potential design application of the proposed approach in a portfolio optimization of two vehicles from the same producer. While the spatial panel modeling approach exists in econometrics, applying this approach to support engineering decisions by considering spatiotemporal heterogeneity and introducing engineering attributes in demand forecasting is the contribution of this work. Our paper is focused on presenting the approach rather than the results per se.

A Mesh-Based Approach for Computational Fluid Dynamics-Free Aerodynamic Optimisation of Complex Geometries Using Area Ruling

In this paper, an optimisation procedure is introduced that uses a significantly cheaper, and CFD-free, objective function for aerodynamic optimisation than conventional CFD-driven approaches. Despite the reduced computational cost, we show that this approach can still drive the optimisation scheme towards a design with a similar reduction in drag coefficient for wave drag-dominated problems. The approach used is ‘CFD-free’, i.e., it does not require any computational aerodynamic analysis. It can be applied to geometries discretised using meshes more conventionally used for ‘standard’ CFD-based optimisation approaches. The approach outlined in this paper makes use of the transonic area rule and its supersonic extension, exploiting a mesh-based parameterisation and mesh morphing methodology. The paper addresses the following question: ‘To what extent can an optimiser perform (wave) drag minimisation if using ‘area ruling’ alone as the objective (fitness) function measurement?’. A summary of the wave drag approximation in transonic and supersonic regimes is outlined along with the methodology for exploiting this theory on a typical CFD surface mesh to construct an objective function evaluation for a given geometry. The implementation is presented including notes on the considerations required to ensure stability, and error minimisation, of the numerical scheme. The paper concludes with the results from a number of (simple and complex geometry) examples of a drag-minimisation optimisation study and the results are compared with an approach using full-fidelity CFD simulation. The overall conclusions from this study suggest that the approach presented is capable of driving a geometry towards a similar shape to when using full-fidelity CFD at a significantly lower computational cost. However, it cannot account for any constraints, driven by other aerodynamic factors, that might be present within the problem.