Optical Sensor For Hydrogen Peroxide Research Articles

Optical hydrogen peroxide sensor for measurements in flow

An optical hydrogen peroxide (H2O2) sensor is presented. The sensor is based on catalytic degradation of H2O2 and the detection of produced oxygen (O2) with a phosphorescent sensor. A novel aspect of the sensor is removal O2 from the analyte solution in flow. This allows the use of a more sensitive O2 sensor. Thereby, a better resolution at lower H2O2 concentrations is achieved. Sensor spots are integrated in a flow-through cell, and H2O2 is measured in flow between 10 and 80 µL/min. The catalytic activities of previously reported catalyst are tested. RuO2 and silica supported platinum nanoparticles are applied in the sensor with limit of detections of 0.16 and 0.17 µM H2O2, respectively. The sensor can measure reliably between 1 and 200 µM H2O2. The concentration range can be extended to 1000 µM H2O2 by exchanging the O2 sensor. Interfering species (NaONOO, NaOCl, t-BuOOH, and ascorbic acid) are tested and show only minor cross-sensitivities. The sensor is applied at-line in a model batch reactor with glucose oxidase to showcase production of H2O2 from glucose.

Photo-physical studies of ultrasmall upconversion nanoparticles embedded organometallic complexes: Probing a dual mode optical sensor for hydrogen peroxide

Here, we report an upconversion nanoparticles (UCNPs) embedded organomettalic complex for detection of hydrogen peroxide (H2O2). The structural as well as the optical properties of the hybrid nanoparticles (HNPs) have been studies to confirm the interaction as well as energy transfer mechanism. The structural characterization confirm the weak interacting force between the UCNPs and the organic complex in HNPs. The optical studies reveal that, HNPs show characteristic optical behaviour of both the phases i.e. it gives an intense downconverted red emission under UV excitation, due to the presence of organic part (Eu3+ ion) as well as an efficient blue upconverted emission from inorganic part (Tm3+ ion) under NIR excitation. The energy transfer between the inorganic (Tm3+ ion) and the organic (Eu3+ ion) material has been observed, which confirms the effective attachment of both the phases in HNPs. The upconversion and downconversion emission properties, have been have successfully utilized for dual mode H2O2 detection.

Diffusion rate of hydrogen peroxide through water-swelled polyurethane membranes

In our efforts to design an optical sensor for hydrogen peroxide, we have realized the importance of knowing/controlling the diffusion of hydrogen peroxide to the indicator, especially in the case of an irreversible indicator. Since we found little literature data, we decided to test the permeability of hydrogen peroxide in commercially available polymers, focusing on the polyurethanes HydroMed™ and HydroThane™, which are commonly used for immobilization matrices in optical sensors. Measured values are between 5.12 10−9 ± 8.50 10−10 and 2.25 10−6 ± 1.00 10−7 cm2 s−1.

Fully Reversible Optical Sensor for Hydrogen Peroxide with Fast Response.

A fully reversible optical sensor for hydrogen peroxide with fast response is presented. The sensor was fabricated by in situ growing ultrasmall platinum nanoparticles (PtNPs) inside the pores of fibrous silica particles (KCC-1). The nanocomposite was then embedded into a hydrogel matrix and form a sensor layer, the immobilized PtNPs can catalytically convert hydrogen peroxide into molecular oxygen, which is measured via luminescent quenching based oxygen sensor underneath. Owing to the high porosity and permeability of KCC-1 and high local concentration of PtNPs, the sensor exhibits fast response (less than 1 min) and full reversibility. The measurement range of the sensor covers 1.0 μM to 10.0 mM, and a very small amount of sample is required during measurement (200 μL). Because of its high stability, excellent reversibility and selectivity, and extremely fast response, the sensor could fulfill all industry requirements for real-time measurement and fill market vacancy.

The Preparation of an Optical Hydrogen Peroxide Sensor Using Polydopamine as a Sensing Medium

Polydopamine (PDA) is well known as an excellent coating material. It has various novel properties such as good biocompatibility, biodegradability, nontoxicity, easiness of deposit on all types of substrates, and strong stability as a primary natural pigment. In this research, in situ preparation of fluorescent PDA was performed by the addition of hydrogen peroxide (H₂O₂) to dopamine (DA) in the presence of Cu²⁺. DA does not show any fluorescence while the in situ self-initiated PDA has a strong fluorescence at 480 nm with excitation wavelength ranged from 330 nm to 420 nm due to the oxidation of DA by H₂O₂. A novel H₂O₂ sensor was fabricated using PDA as a fluorescence sensing medium. High sensitivity and linear response were achieved with a detection limit of 0.7 μM. The sensing medium is not toxic and allows biodegradation, which indicates high potential for in vivo application.

Ultrasensitive optical sensor for hydrogen peroxide using silver nanoparticles synthesized at room temperature by GQDs

The present study describes the synthesis of silver nanoparticles capped with GQDs (Ag-GQDs) at room temperature for the first time and their application towards the optical sensing of H2O2. Unlike the previous reports, the preparation of Ag-GQDs not involves external heating because more number of hydroxyl groups present in the surface of GQDs synthesized in the present study. The GQDs are prepared from citric acid by pyrolysis at 200°C. The size of the Ag-GQDs is found to be 9±1nm. They show characteristic SPR band at 403nm and show emission maximum at 457nm when excited at 365nm. The emission intensity of Ag-GQDs was drastically quenched when compared to GQDs, which show emission maximum at 451nm at the same excitation. This is due to the masking of free functional groups responsible for emission of GQDs besides the oxidation of hydroxyl groups to aldehyde after reducing Ag+ to Ag0. The synthesized Ag-GQDs were then successfully used for the sensitive determination of H2O2 by spectrofluorimetry. The addition of 0.1μM H2O2 to Ag-GQDs leads to decrease in the absorbance intensity at 403nm besides the yellow color becomes colorless. Interestingly, even the addition of 25nM H2O2 increases the emission intensity at 457nm. The decrease in the absorbance and increase in the fluorescence are attributed to the leaching of Ag+ from the surface of GQDs due to the oxidation of Ag0 to Ag+ by H2O2. Further, the practical application of the present method was demonstrated by determining H2O2 in human urine samples.

Role of capping agents in controlling silver nanoparticles size, antibacterial activity and potential application as optical hydrogen peroxide sensor

Catalytic reaction between PVA-capped AgNPs and hydrogen peroxide, and the corresponding LSPR optical absorbance spectra as a function of time.

Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application.

Hydrogen peroxide (H2O2) is an important member of the reactive oxygen species (ROS) family. Among ROS, H2O2 is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H2O2 within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H2O2 concentrations ranging from 1-100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H2O2 can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H2O2 concentration profile measurements in natural photosynthetic biofilms under light stress reaching H2O2 concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H2O2 during stimulation of neutrophils.

Optical Hydrogen Peroxide Sensor Using Dopamine-Conjugated Quantum Dots as Sensing Medium

Optical Hydrogen Peroxide Sensor Using Dopamine-Conjugated Quantum Dots as Sensing Medium

Synthesis of starch-stabilized silver nanoparticles and their application as a surface plasmon resonance-based sensor of hydrogen peroxide

Stable and uniform starch-stabilized silver nanoparticles with average diameter 14.4 ± 3.3 nm are synthesized via green synthetic procedure, using ultrasound mediated reduction of silver nitrate by d-glucose. UV–vis spectroscopy, high-resolution transmission electron microscopy, X-ray diffraction, thermogravimetric/differential thermal analysis and differential scanning calorimetry are used to completely characterize the starch-stabilized silver nanoparticles. These nanoparticles exhibit a catalytic activity in the reduction of hydrogen peroxide (H 2O 2). The degradation of silver nanoparticles, induced by the catalytic decomposition of hydrogen peroxide, causes a considerable change in the absorbance strength of localized surface plasmon resonance band depending on the H 2O 2 concentration. The characterization and calibration of improvised plasmon resonance-based optical sensor is carried out. A good sensitivity and a linear response over the wide concentration range of 10 −1–10 −6 mol/L H 2O 2 is established. The quantification limit of this sensor is found to be 0.9 μM H 2O 2, which is lower than certain enzyme-based biosensors. Therefore, this optical sensor for hydrogen peroxide can be potentially applied in determination of other reactive oxygen species as well.

Reversible, fluorescence-based optical sensor for hydrogen peroxide

The preparation and characterisation are described of a robust, reversible, hydrogen peroxide optical sensor, based on the fluorescent quenching of the dye ion-pair [Ru(bpy)(3)(2+)(Ph(4)B(-))(2)], by O(2) produced by the catalytic breakdown of H(2)O(2), utilizing the inorganic catalyst RuO(2).xH(2)O. The main feature of this system is the one-pot formulation of a coating ink that, when dried, forms an active single-layer fluorescence-based H(2)O(2) sensor, demonstrably capable of detecting H(2)O(2) over the range of 0.01 to 1 M, with a relative standard deviation of ca. 4% and a calculated lower limit of detection of 0.1 mM. These sensors are sterilisable, using dry-heat, and stable when stored over 40 days, without exhibiting any loss in sensitivity or response characteristics.

Reversible Optical Sensor Membrane for Hydrogen Peroxide Using an Immobilized Fluorescent Probe, and its Application to a Glucose Biosensor

An optical sensor for hydrogen peroxide (HP) based on the probe europium tetracycline (EuTc) incorporated into a polyacrylonitrile-co-polyacryl- amide polymer matrix is described. Upon optical exci- tation with 400-nm light, the EuTc in the membrane displays fairly strong fluorescence peaking at 616 nm. The fluorescence intensity increases up to 3-fold if the sensor is exposed to solutions containing HP. The effect is reversible and can thus be used for continuous sen- sing. The largest signal changes with HP are observed at pH levels between 6.5 and 7.5, and the range of the response is between 10 and 300 ppm (w=w) of HP, equal to 0.3 to � 10 mmol L � 1 ). At concentrations above 0.3% of HP, decomposition of the EuTc in the membrane is observed. The limit of detection is � 15 ppm (0.45 mmol L � 1 ). The response is fully reversible and rather slow (� 10 min) in both directions, but the reverse response may be accelerated by addition of a reducing agent such as thiosulfate. Alkali ions and most anions remain inert, but phosphate and citrate interfere, as do Cu 2þ ions, which quench fluorescence. In order to image the spatial distribution of HP concentrations, sensor membranes were placed on the bottom of the wells of a microtiter plate, and their fluorescence was imaged using an LED-based device based on the mea- surement of the luminescence decay time of EuTc. If glucose oxidase is immobilized on the sensor layer, a glucose sensor is obtained in which the HP sensor acts as the transducer and which can quantify glucose in concentrations between 0.1 and 5 mmol L � 1 . The limit of detection for glucose is � 0.2 mmol L � 1 .

Optical sensor for hydrogen peroxide

Three types of sensors for continuous determination of hydrogen peroxide (HP) are described. The working principles are based on the decomposition of HP by a catalyst and on the measurement of the amount of oxygen thereby produced. The change in oxygen tension is quantitatively determined via the quenching of the fluorescence of a silica gel-adsorbed dye entrapped in silicone rubber. Three methods were found to be useful for HP decomposition. In the first one, the enzyme catalase (which acts as the catalyst) is co-adsorbed onto silica gel and thus is in the same phase as the indicator. In the second one, the enzyme and the dye are adsorbed on different silica gel particles which then are incorporated into the polymer layer. In the third one, finely dispersed silver powder (another catalyst) is embedded in a silicone rubber layer that is spread over the oxygen sensing membrane. The sensor is capable of continuously recording HP in the 0.1 to 10.0 mM concentration range, with a precision of ±0.1 mM at 1 mM HP. Its response time varies from 2.5 to 5 min.