AbstractIn the atomic comagnetometer, nuclear spin relaxation is a core parameter that affects the magnetic field suppression ability and stability, and is influenced by various gradient fields. It is necessary to measure and separate the effects of different gradients on nuclear spin, which helps to effectively suppress them separately. This article proposes a periodic optical pumping method for separating and measuring polarization gradient relaxation and magnetic field gradient relaxation, considering the effects of pump light and applied magnetic field. The comprehensive influence model for pump light on transverse nuclear spin relaxation is established, and the measurement steps and parameter selection criteria for the proposed method considering signal decay characteristics are provided. Furthermore, the proportion of various gradient relaxations is quantified. This work provides an evaluation method for gradient relaxation suppression, supporting the improvement of the measurement sensitivity and stability of the atomic comagnetometer.