Optical Label Research Articles

High‐contrast QPSK pattern recognition device consisting of a 4×$\times$4 MMI coupler

AbstractPattern recognition of optical label is utilized not only in packet switches but also in the field of data security due to its wide range of applications. In particular, pattern recognition using waveguide devices is highly valued because it eliminates the need for encoder processing. However, existing methods suffer from the problem of degraded contrast ratio because half of the input power are emitted from non‐target waveguides. In this article, a method is proposed to improve the contrast ratio employing a 44 multi‐mode interference (MMI) coupler with multiple input signals. The theoretical results reveal that the proposed method improves the contrast ratio from 3.0 dB to 9.5 dB and dB for the three‐input and four‐input cases, respectively, for quadrature phase‐shift keying (QPSK)‐modulated signal. Furthermore, numerical simulation was performed through the three dimension finite‐difference time‐domain (3D‐FDTD) method, and the proposed scheme successfully discriminated each QPSK pattern. The power value at all ports closely matches the theoretical value, and the ratios of 9.5 dB and 30.0 dB are numerically obtained.

Background-Free Imaging of Food Freshness Using Curcumin-Functionalized Upconversion Reversible Hydrogel Patch.

Functionalized upconversion nanomaterials can overcome the drawbacks faced of strong background interference, photodamage, and spectral overlap by conventional optical labeling. Here, curcumin-functionalized upconversion hydrogel patch is designed with background-free and reversible for food freshness monitoring by ultra-sensitive response to biogenic amines. By loading the probes onto hydrogel patch, utilizing the good ductility to solve the problem of non-smooth surface coverage, thus accurately capturing biogenic amines. The presence of biogenic amines leads to the conversion of the diketone group on the probe to enolate ions, which triggers fluorescence resonance energy transfer (FRET) and ultimately causes the upconverted fluorescence to gradually change from green to red. The probe exhibits good detection capability for biogenic amines with a low limit of detection (LOD) of 2.73 µm. Interestingly, the patch can be restored to its initial state after water rinsing, realizing reversible detection of biogenic amines. Additionally, combining the color recognition system of smartphone can convert the imaging signal into a data signal to achieve quantitative analysis and show a reliable assessment comparable to the results of high performance liquid chromatography (HPLC). This study demonstrates the practical applicability in real-time monitoring of freshness, suggests great potential in developing optical nano-sensing strategy to ensure food safety.

Magnetic Supraparticles as Identifiers in Single-Layer Lithium-Ion Battery Pouch Cells.

The development of effective recycling technologies is essential for the recovery and reuse of the raw materials required for lithium-ion batteries (LIBs). Future recycling processes depend on accessible information, necessitating the implementation of a digital battery passport. The European battery regulation mandates the use of a machine-readable identifier physically attached to the batteries for accessing digital information. Since externally applied optical labels are vulnerable to mechanical damage, technologies for identification without these restrictions could be beneficial. This study demonstrates that magnetic supraparticles (SPs) can be used for contactless identification of lithium nickel manganese cobalt oxide (NMC) battery pouch cells via magnetic particle spectroscopy (MPS) and that multiple pouch cells can be discriminated based on their specific magnetic code. A comparison of three independent model scenarios revealed that the detection of SPs and the impact on cell performance are dependent on the integration location. The results validate the concept of magnetic identification in metallic environments with MPS as an alternative to optical labeling methods. This study provides a foundation for the development of a new selective labeling and identification technology for batteries, with the potential to facilitate recycling and contribute to a more sustainable future.

Kiwi-Inspired Rational Nanoarchitecture with Intensified and Discrete Magneto-Fluorescent Functionalities for Ultrasensitive Point-of-Care Immunoassay.

Fluorescent lateral flow immunoassays (FLFIA) is a well-established rapid detection technique for quantitative analysis. However, achieving accurate analysis of biomarkers at the pg mL-1 level using FLFIA still poses challenges. Herein, an ultrasensitive FLFIA platform is reported utilizing a kiwi-type magneto-fluorescent silica nanohybrid (designated as MFS) that serves as both a target-enrichment substrate and an optical signal enhancement label. The spatially-layered architecture comprises a Fe3O4 core, an endocarp-fibers like dendritic mesoporous silica, seed-like quantum dots, and a kiwi-flesh like silica matrix. The MFS demonstrates heightened fluorescence brightness, swift magnetic response, excellent size uniformity, and dispersibility in water. Through liquid-phase capturing and fluorescence-enhanced signal amplification, as well as magnetic-enrichment sample amplification and magnetic-separation noise reduction, the MFS-based FLFIA is successfully applied to the detection of cardiac troponin I that achieved a limit of detection at 8.4 pg mL-1, tens of times lower than those of previously published fluorescent and colorimetric lateral flow immunoassays. This work offers insights into the strategic design of magneto-fluorescent synergetic signal amplification on LFIA platform and underscores their prospects in high-sensitive rapid and on-site diagnosis of biomarkers.

Rydberg-State Double-Well Potentials of Van der Waals Molecules

Recent progress in studies of Rydberg double-well electronic energy states of MeNg (Me = 12-group atom, Ng = noble gas atom) van der Waals (vdW) molecules is presented and analysed. The presentation covers approaches in experimental studies as well as ab initio-calculations of potential energy curves (PECs). The analysis is shown in a broader context of Rydberg states of hetero- and homo-diatomic molecules with PECs possessing complex ‘exotic’ structure. Laser induced fluorescence (LIF) excitation spectra and dispersed emission spectra employed in the spectroscopical characterization of Rydberg states are presented on the background of the diverse spectroscopic methods for their investigations such as laser vaporization–optical resonance (LV-OR), pump-and-probe methods, and polarization labelling spectroscopy. Important and current state-of-the-art applications of Rydberg states with irregular potentials in photoassociation (PA), vibrational and rotational cooling, molecular clocks, frequency standards, and molecular wave-packet interferometry are highlighted.

Programmable Persistent Room Temperature Phosphorescence Switches through Wavelength-Selective Photoactivation.

Controlling multicolor persistent room-temperature phosphorescence (RTP) through photoirradiation holds fundamental significance but remains a significant challenge. In this study, we engineered a wavelength-selective photoresponsive system utilizing the Förster resonance energy transfer strategy. This system integrates a photoactivated long-lived luminescent material as the energy donor with a fluorescent photoswitch as the energy acceptor, facilitating programmable persistent luminescence switches. Distinct afterglow color states, such as initial nonemissive, green, yellow, and orange, were achieved through irradiation at 400 nm, 365 nm, and 254 nm, respectively. Based on this capability, we established an interacting network for multistate afterglow color switching among these four emissive states. In addition, we demonstrate the potential of this wavelength-selective photoresponsive system in the photo-controlled rewritable printing of multicolor afterglow images on a single thin film. This work represents a substantial step towards the fabrication of sophisticated wavelength-selective photoresponsive systems, potentially revolutionizing applications in optical data storage, security labeling, and smart displays by enabling precise control over photoresponsive behaviors under various photoirradiation wavelengths.

Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies.

The prevalence of allergic diseases has increased tremendously in recent decades, which can be attributed to growing exposure to environmental triggers, changes in dietary habits, comorbidity, and the increased use of medications. In this context, the multiplexed diagnosis of sensitization to various allergens and the monitoring of the effectiveness of treatments for allergic diseases become particularly urgent issues. The detection of allergen-specific antibodies, in particular, sIgE and sIgG, is a modern alternative to skin tests due to the safety and efficiency of this method. The use of allergen microarrays to detect tens to hundreds of allergen-specific antibodies in less than 0.1 mL of blood serum enables the transition to a deeply personalized approach in the diagnosis of these diseases while reducing the invasiveness and increasing the informativeness of analysis. This review discusses the technological approaches underlying the development of allergen microarrays and other protein microarrays, including the methods of selection of the microarray substrates and matrices for protein molecule immobilization, the obtainment of allergens, and the use of different types of optical labels for increasing the sensitivity and specificity of the detection of allergen-specific antibodies.

Physical Unclonable Functions with Hyperspectral Imaging System for Ultrafast Storage and Authentication Enabled by Random Structural Color Domains.

Physical unclonable function (PUF) is attractive in modern encryption technologies. Addressing the disadvantage of slow data storage/authentication in optical PUF is paramount for practical applications but remains an on-going challenge. Here, a highly efficient PUF strategy based on random structural color domains (SCDs) of cellulose nanocrystal (CNC) is proposed for the first time, combing with hyperspectral imaging system (HIS) for ultrafast storage and authentication. By controlling the growth and fusion behavior of the tactoids of CNC, the SCDs display an irregular and random distribution of colors, shapes, sizes, and reflectance spectra, which grant unique and inherent fingerprint-like characteristics that are non-duplicated. Based on images and spectra, these fingerprint features are used to develop two sets of PUF key generation methods, which can be respectively authenticated at the user-end and the manufacturer-front-end that achieving a high coding capacity of at least 22304. Notably, the use of HIS greatly shortens the time of key reading and generation (≈5s for recording, 0.5-0.7s for authentication). This new optical PUF labels can not only solve slow data storage and complicated authentication in optical PUF, but also impulse the development of CNC in industrial applications by reducing color uniformity requirement.

DIPG-73. FOCUSED ULTRASOUND FOR TREATMENT OF CHILDREN DIAGNOSED WITH DIFFUSE MIDLINE GLIOMAS

Abstract BACKGROUND Diffuse midline glioma (DMG) is a highly aggressive and fatal brain tumor that predominantly impacts children and young adults. The infiltrative pattern and rapid growth characteristics of DMG causes a significant challenge in finding an effective treatment. Additionally, the blood-brain barrier (BBB) poses a significant challenge by restricting the passage of therapeutic drugs to tumor bed. Currently the established standard of care is radiotherapy (RT), which is transitory. Thus there is a need for improved treatment options. OBJECTIVE Focused ultrasound (FUS) is an advancing non-invasive technology that has been used to open BBB and enhance drug delivery. We hypothesize that a combination therapy of FUS with pharmacological agents will be an effective and non-invasive treatment option for children diagnosed with DMG. METHOD Syngeneic DMG murine models were used to study the combination therapy of FUS with ONC201, FUS with anti-PD1 and FUS with RT. FUS mediated BBB opening was monitored with contrast-enhanced T1-weighted MRI. FUS-mediated drug delivery was validated with western blot, assessment of ROS, and optical label imaging. Kaplan Meier analysis was used to assess survival benefits. Single cell RNA-sequencing (scRNA-Seq) analysis is being done to assess the treatment effects of FUS and RT combination therapy. RESULTS/CONCLUSION We found that FUS and ONC201 combination therapy resulted in a decrease in NDUFA12 protein (a biomarker of ONC201 response) and an increase in ROS production compared to ONC201 monotherapy. For FUS and anti-PD1 combination therapy, an increased anti-PD1 delivery to the brainstem was detected. The overall survival of mice treated with FUS and anti-PD1 combination therapy increased when compared to anti-PD1 alone. Our preliminary scRNA-Seq analysis showed that combination therapy of FUS and RT induces innate immune response compared to the untreated group. Combination therapy with focused ultrasound shows a promising outcome by increasing the efficacy of therapeutic drugs and immunotherapy.

Hollow versatile Ag@Pt alloy nanoparticles with nanozyme activity for detection and photothermal sterilization of Helicobacter pylori.

In view of a large number of people infected with Helicobacter pylori (H. pylori) with great harm followed, there is an urgent need to develop a non-invasive, easy-to-operate, and rapid detection method, and to identify effective sterilization strategies. In this study, highly specific nanoprobes with nanozyme activity, Ag@Pt nanoparticles (NPs) with the antibody, were utilized as a novel lateral flow immunoassay (LFIA). The optical label (Ag@Pt NPs) was enhanced by the introduction of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) and compared with a gold nanoparticles (Au NPs) optical label. Under the optimal condition, Ag@Pt-LFIA and TMB-enhanced Ag@Pt-LFIA for H. pylori were successfully established, two of which were over twofold and 100-fold more sensitive than conventional visual Au NP-based LFIA, respectively. Furthermore, Ag@Pt NPs with the antibody irradiated with NIR laser (808nm) at a power intensity of 550 mW/cm2 for 5min exhibited a remarkable antibacterial effect. Thenanoprobes could close to bacteria through effective interactions between antibodies and bacteria, thereby benefiting photothermal sterilization. Overall, Ag@Pt NPs provide promising applications in pathogen detection and therapeutic applications.

Designing SERS nanotags for profiling overexpressed surface markers on single cancer cells: A review

This review focuses on the chemical design and the use of Surface-Enhanced Raman Scattering (SERS)-active nanotags for measuring surface markers that can be overexpressed at the surface of single cancer cells. Indeed, providing analytical tools with true single-cell measurements capabilities is capital, especially since cancer research is increasingly leaning toward single-cell analysis, either to guide treatment decisions or to understand complex tumor behaviour including the single-cell heterogeneity and the appearance of treatment resistance. Over the past two decades, SERS nanotags have triggered significant interest in the scientific community owing their advantages over fluorescent tags, mainly because SERS nanotags resist photobleaching and exhibit sharper signal bands, which reduces possible spectral overlap and enables the discrimination between the SERS signals and the autofluorescence background from the sample itself. The extensive efforts invested in harnessing SERS nanotags for biomedical purposes, particularly in cancer research, highlight their potential as the next generation of optical labels for single-cell studies. The review unfolds in two main parts. The first part focuses on the structure of SERS nanotags, detailing their chemical composition and the role of each building block of the tags. The second part explores applications in measuring overexpressed surface markers on single-cells. The latter encompasses studies using single nanotags, multiplexed measurements, quantitative information extraction, monitoring treatment responses, and integrating phenotype measurements with SERS nanotags on single cells isolated from complex biological matrices. This comprehensive review anticipates SERS nanotags to persist as a pivotal technology in advancing single-cell analytical methods, particularly in the context of cancer research and personalized medicine.

Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels

Tracing individual cell pathways among the whole population is crucial for understanding their behavior, cell communication, migration dynamics, and fate. Optical labeling is one approach for tracing individual cells, but it typically requires genetic modification to induce the generation of photoconvertible proteins. Nevertheless, this approach has limitations and is not applicable to certain cell types. For instance, genetic modification often leads to the death of macrophages. This study aims to develop an alternative method for labeling macrophages by utilizing photoconvertible micron-sized capsules capable of easy internalization and prolonged retention within cells. Thermal treatment in a polyvinyl alcohol gel medium is employed for the scalable synthesis of capsules with a wide range of fluorescent dyes, including rhodamine 6G, pyronin B, fluorescein, acridine yellow, acridine orange, thiazine red, and previously reported rhodamine B. The fluorescence brightness, photostability, and photoconversion ability of the capsules are evaluated using confocal laser scanning microscopy. Viability, uptake, mobility, and photoconversion studies are conducted on RAW 264.7 and bone marrow-derived macrophages, serving as model cell lines. The production yield of the capsules is increased due to the use of polyvinyl alcohol gel, eliminating the need for conventional filtration steps. Capsules entrapping rhodamine B and rhodamine 6G meet all requirements for intracellular use in individual cell tracking. Mass spectrometry analysis reveals a sequence of deethylation steps that result in blue shifts in the dye spectra upon irradiation. Cellular studies on macrophages demonstrate robust uptake of the capsules. The capsules exhibit minimal cytotoxicity and have a negligible impact on cell motility. The successful photoconversion of RhB-containing capsules within cells highlights their potential as alternatives to photoconvertible proteins for individual cell labeling, with promising applications in personalized medicine.

QR codes and automated decision-making in the COVID-19 pandemic

In this article, we explore Quick Response (QR) codes (machine-readable optical labels that link to information) and how, after a period of having fallen out of favor, they have been reactivated and have come to underpin COVID-19 automation and contact-tracing efforts. During the pandemic, they were used especially for “safe entry” and other kinds of check-in to locations to facilitate contact tracing. In this context, QR codes facilitate automated decision-making in relation to infectious disease surveillance and disease outbreak control. However, the use of QR codes for contact-tracing purposes has enjoyed mixed success and its implementation has encountered several challenges, as we illustrate through a case study that explores QR codes and COVID contact tracing in Singapore and Australia.

Preparation of photoresponsive lanthanide aluminate-immobilized nanofibers from recycled bioplastic via solution blowing spinning for optical authentication labeling

Photochromism is an intriguing technology to enhance the authentication of various commercial materials. In order to produce a transparent authentication film, it is also significant to improve the engineering process of anti-counterfeiting materials. In contrast to electrospinning, the solution blowing spinning (SBS) technique has been recently developed to produce nanofibers at low-cost and high yield with no need to apply high voltage. It has been essential to reduce the toxic pollution caused by plastic waste. Herein, we develop a photoluminescent nanofibrous membrane from recycled polylactic acid (PLA) waste and nanoparticles of strontium aluminate (NSA) by using the simple SBS technique. We investigated the potential use of the NSA@PLA composite nanofibers for photochromic anti-counterfeiting driven by ultraviolet light. Encapsulating the nanoparticle form of the strontium aluminate phosphor into polylactic acid nanofibers is necessary to ensure the transparent characteristic of the nanofibrous film. The film transparency can be further improved by the efficient dispersion of NSA without aggregation in the polylactic acid solution. Nanofibrous membranes with varying emission characteristics were produced by varying the NSA ratio. Both of X-ray diffraction (XRD) and Transmission electron microscopy (TEM) demonstrated the morphology of NSA to show diameters of 5–12 nm. The morphologies of the nanofibrous membranes were examined using various analytical methods, including energy-dispersive X-ray spectroscopy (EDX), X-ray fluorescence spectroscopy (XRF) and scanning electron microscopy (SEM). The mechanical characteristics and optical luminescence were also examined. SEM analysis of the NSA@PLA nanofibers showed diameters of 100–220 nm. The NSA@PLA nanofibrous membranes exhibited an emission peak at 518 nm upon excitation at 365 nm. The contact angles of the NSA@PLA nanofibrous membranes increased from 138.2° to 144.5° as the NSA concentration was increased, indicating an improved superhydrophobicity. Exposure to ultraviolet light caused the transparent nanofibers to undergo a rapid and strongly reversible photochromism to greenish emission. The present study can be reported as an easy and efficient approach to create anti-counterfeiting materials that offer a perfect market with social and financial profits.

Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging.

Despite the numerous clearing techniques that emerged in the last decade, processing postmortem human brains remains a challenging task due to its dimensions and complexity, which make imaging with micrometer resolution particularly difficult. This paper presents a protocol to perform the reconstruction of volumetric portions of the human brain by simultaneously processing tens of sections with the SHORT (SWITCH - H2O2 - Antigen Retrieval - 2,2'-thiodiethanol [TDE]) tissue transformation protocol, which enables clearing, labeling, and sequential imaging of the samples with light-sheet fluorescence microscopy (LSFM). SHORT provides rapid tissue clearing and homogeneous multi-labeling of thick slices with several neuronal markers, enabling the identification of different neuronal subpopulations in both white and grey matter. After clearing, the slices are imaged via LSFM with micrometer resolution and in multiple channels simultaneously for a rapid 3D reconstruction. By combining SHORT with LSFM analysis within a routinely high-throughput protocol, it is possible to obtain the 3D cytoarchitecture reconstruction of large volumetric areas at high resolution in a short time, thus enabling comprehensive structural characterization of the human brain.

Acetic acid-driven synthesis of environmentally stable MAPb0.5Sn0.5Br3 nano-assembly for anti-counterfeiting

In mixed Sn-Pb perovskites, the synergistic properties of tin (Sn) and lead (Pb) are leveraged, effectively combining the merits of Pb-based perovskites while simultaneously reducing Pb-associated toxicity. However, the propensity for Sn to undergo facile oxidation from Sn2+ to Sn4+ poses a significant challenge to the stability of these mixed perovskites, limiting their advancement. This study proposes an innovative acetic acid (HAc)-driven synthesis approach to obtain a stable chain-like MAPb0.5Sn0.5Br3 nano-assembly. Leveraging the acidic properties of HAc serves a dual purpose. Primarily, it curtails the oxidation of Sn2+ to Sn4+. Secondly, it orchestrates nanocrystals (NCs) into a more uniform and ordered chain-like assembly, a consequence of hydrogen bonding and coordination interactions facilitated by the HAc. Additionally, HAc demonstrates its capability to passivate MAPb0.5Sn0.5Br3 surface through coordination bonding with unsaturated sites (i.e., Sn2+ or Pb2+), thus effectively compensating for bromide vacancies. Introducing HAc during the synthesis process yields perovskite NCs with enhanced thermal resilience, optical and water stability. Drawing upon the different stimulus responses of synthesized perovskite NCs when exposed to external environment, the optical anti-counterfeiting labels are prepared. The findings provide a potent strategy for augmenting the stability of perovskite NCs, suggesting their potential applicability in anti-counterfeiting endeavors.

Photoinduced Transition from Quasi-Two-Dimensional Ruddlesden-Popper to Three-Dimensional Halide Perovskites for the Optical Writing of Multicolor and Light-Erasable Images.

Optical data storage, information encryption, and security labeling technologies require materials that exhibit local, pronounced, and diverse modifications of their structure-dependent optical properties under external excitation. Herein, we propose and develop a novel platform relying on lead halide Ruddlesden-Popper phases that undergo a light-induced transition toward bulk perovskite and employ this phenomenon for the direct optical writing of multicolor patterns. This transition causes the weakening of quantum confinement and hence a reduction in the band gap. To extend the color gamut of photoluminescence, we use mixed-halide compositions that exhibit photoinduced halide segregation. The emission of the films can be tuned across the range of 450-600 nm. Laser irradiation provides high-resolution direct writing, whereas continuous-wave ultraviolet exposure is suitable for recording on larger scales. The luminescent images created on such films can be erased during the visualization process. This makes the proposed writing/erasing platform suitable for the manufacturing of optical data storage devices and light-erasable security labels.

Flexible and accurate system calibration method in microscopic fringe projection profilometry.

The three-dimensional (3D) measurement task of complex microstructures holds paramount significance in the domains of precision manufacturing and inspection. The calibration of the 3D system heavily determines the final reconstruction accuracy. The widely adopted system calibration method is phase-height mapping (PHM) and stereo vision (SV) based. The former can be applied directly to the calculation without considering the imaging model of the system, but it relies on highly precise and expensive translation stages or standard blocks. The latter's accuracy cannot be guaranteed because it is difficult to accurately calibrate the projector. In this paper, we establish an optically coupled microscopic fringe projection profilometry system that consists of a Scheimpflug pinhole projector and a super-low distortion bi-telecentric camera. We introduce a simplified 3D system calibration approach that combines phase modulation transfer and ray propagation. Our method enables the simultaneous calibration of the system, including the calibration of the projector, camera, and the phase to a 3D coordinates relationship, using only a 2D target. The calibrated projector's external parameters are used to obtain the target's complete poses, and then the direct mapping coefficients of the phase to the 3D coordinates can be obtained through the optical geometry structure and phase labels. Comparable experiments verify the feasibility of the proposed method.

Adverse Weather Optical Flow: Cumulative Homogeneous-Heterogeneous Adaptation.

Optical flow has made great progress in clean scenes, while suffers degradation under adverse weather due to the violation of the brightness constancy and gradient continuity assumptions of optical flow. Typically, existing methods mainly adopt domain adaptation to transfer motion knowledge from clean to degraded domain through one-stage adaptation. However, this direct adaptation is ineffective, since there exists a large gap due to adverse weather and scene style between clean and real degraded domains. Moreover, even within the degraded domain itself, static weather (e.g., fog) and dynamic weather (e.g., rain) have different impacts on optical flow. To address above issues, we explore synthetic degraded domain as an intermediate bridge between clean and real degraded domains, and propose a cumulative homogeneous-heterogeneous adaptation framework for real adverse weather optical flow. Specifically, for clean-degraded transfer, our key insight is that static weather possesses the depth-association homogeneous feature which does not change the intrinsic motion of the scene, while dynamic weather additionally introduces the heterogeneous feature which results in a significant boundary discrepancy in warp errors between clean and degraded domains. For synthetic-real transfer, we figureout that cost volume correlation shares a similar statistical histogram between synthetic and real degraded domains, benefiting to holistically aligning the homogeneous correlation distribution for synthetic-real knowledge distillation. Under this unified framework, the proposed method can progressively and explicitly transfer knowledge from clean scenes to real adverse weather. In addition, we further collect a real adverse weather dataset with manually annotated optical flow labels and perform extensive experiments to verify the superiority of the proposed method. Both the code and the dataset will be available at https://github.com/hyzhouboy/CH2DA-Flow.

WDM Optical Network Monitoring Technology Based on PAM4 Digital Optical Label

WDM Optical Network Monitoring Technology Based on PAM4 Digital Optical Label