Optical Fiber-based Techniques Research Articles

Diffuse Reflectance Spectroscopy of Changes in Tumor Microenvironment in Response to Different Doses of Radiation.

Radiation therapy plays an important role in cancer treatment, as it is an established method used as part of the treatment plan for the majority of cancer patients. Real-time monitoring of the effects of radiation on the tumor microenvironment can contribute to the development of better treatment plans. In this study, we use diffuse reflectance spectroscopy, a non-invasive optical fiber-based technique, to determine the effects of different doses of radiation on the tumor microenvironment, as well as to determine the sensitivity of diffuse reflectance spectroscopy to low doses of radiation that are used in the treatment of certain cancers. We injected 4T1 cells into 50 Balb/c mice to generate tumor xenografts. When the tumors grew to 200 mm3, we distributed the mice into a control group or one of three radiation groups: 1, 2, or 4 Gy/fraction, and they underwent treatment for five consecutive days. We measured the tumor volume and collected diffuse reflectance spectra every day, with optical measurements being acquired both before and one h postirradiation on the five days of treatment. Based on the diffusely reflected light, we quantified vascular oxygenation, total hemoglobin content, and tissue scattering within these tumors. There was a significant increase in tumor vascular oxygenation, which was primarily due to an increase in oxygenated hemoglobin, in response to a 1 Gy/fraction of radiation, while there was a decrease in tissue scattering in response to all doses of radiation. Immunohistochemical analysis revealed that tumor cell proliferation and apoptosis were higher in irradiated groups compared to the control group. Our findings show that diffuse reflectance spectroscopy is sensitive to microenvironmental changes in tumors treated with doses of radiation as low as 1 Gy/fraction.

Method for accurate registration of tissue autofluorescence imaging data with corresponding histology: a means for enhanced tumor margin assessment.

An important step in establishing the diagnostic potential for emerging optical imaging techniques is accurate registration between imaging data and the corresponding tissue histopathology typically used as gold standard in clinical diagnostics. We present a method to precisely register data acquired with a point-scanning spectroscopic imaging technique from fresh surgical tissue specimen blocks with corresponding histological sections. Using a visible aiming beam to augment point-scanning multispectral time-resolved fluorescence spectroscopy on video images, we evaluate two different markers for the registration with histology: fiducial markers using a 405-nm CW laser and the tissue block's outer shape characteristics. We compare the registration performance with benchmark methods using either the fiducial markers or the outer shape characteristics alone to a hybrid method using both feature types. The hybrid method was found to perform best reaching an average error of 0.78±0.67 mm. This method provides a profound framework to validate diagnostical abilities of optical fiber-based techniques and furthermore enables the application of supervised machine learning techniques to automate tissue characterization.

Optical Fiber Sensors in Physical Intrusion Detection Systems: A Review

Fiber optic sensors have become a mainstream sensing technology within a large array of applications due to their inherent benefits. They are now used significantly in structural health monitoring, and are an essential solution for monitoring harsh environments. Since their first development over 30 years ago, they have also found promise in security applications. This paper reviews all of the optical fiber-based techniques used in physical intrusion detection systems. It details the different approaches used for sensing, interrogation, and networking, by research groups, attempting to secure both commercial and residential premises from physical security breaches. The advantages and the disadvantages of the systems are discussed, and each of the different perimeter protection methods is outlined, namely, in-ground, perimeter fence, and window and door protection. This paper reviews the progress in optical fiber-based intrusion detection techniques from the past through to the current state-of-the-art systems and identifies areas, which may provide opportunities for improvement, as well as proposing future directions in this field.

Specialty Fibers for Terahertz Generation and Transmission: A Review

Terahertz (THz) frequency range, lying between the optical and microwave frequency ranges covers a significant portion of the electro-magnetic spectrum. Though its initial usage started in the 1960s, active research in the THz field started only in the 1990s by researchers from both optics and microwaves disciplines. The use of optical fibers for THz application has attracted considerable attention in recent years. In this paper, we review the progress and current status of optical fiber-based techniques for THz generation and transmission. The first part of this review focuses on THz sources. After a review on various types of THz sources, we discuss how specialty optical fibers can be used for THz generation. The second part of this review focuses on the guided wave propagation of THz waves for their transmission. After discussing various wave guiding schemes, we consider new fiber designs for THz transmission.

Optical fiber-based in vivo quantification of growth factor receptors

Growth factor receptors such as epidermal growth factor receptor 1 and human epidermal growth receptor 2 (HER2) are overexpressed in certain cancer cells. Antibodies against these receptors (eg. cetuximab and transtuzumab [Herceptin]) have shown therapeutic value in cancer treatment. The existing methods for the quantification of these receptors in tumors involve immunohistochemistry or DNA quantification, both in extracted tissue samples. The goal of the study was to evaluate whether an optical fiber-based technique can be used to quantify the expression of multiple growth factor receptors simultaneously. The authors examined HER2 expression using the monoclonal antibody trastuzumab as a targeting ligand to test their system. They conjugated trastuzumab to 2 different Alexa Fluor dyes with different excitation and emission wavelengths. Two of the dye conjugates were subsequently injected intravenously into mice bearing HER2-expressing subcutaneous tumors. An optical fiber was then inserted into the tumor through a 30-gauge needle, and using a single laser beam as the excitation source, the fluorescence emitted by the 2 conjugates was identified and quantified by 2-photon optical fiber fluorescence. The 2 conjugates bound to the HER2-expressing tumor competitively in a receptor-specific fashion, but they failed to bind to a similar cell tumor that did not express HER2. The concentration of the conjugate present in the tumor as determined by 2-photon optical fiber fluorescence was shown to serve as an index of the HER2 expression levels. These studies offer a minimally invasive technique for the quantification of tumor receptors simultaneously.

Delamination detection in an I-section glass fiber reinforced plastic beam with an opticalfiber-based stress wave method

An optical fiber-based technique for the detection of the delamination at the web/flangejunction of an I-section glass fiber reinforced plastic beam is presented. A fiber opticalinterferometric sensor is adhered to the surface of the web to serve as a stress wavereceiver, and a piezoelectric transmitter (PZT) is employed to produce a stresswave in the structure. Several methods are applied to detect the delamination,and summing the frequency spectra from multiple measurements is proved to beeffective. From the frequency components of the sensor output corresponding tovarious positions of the PZT, the delamination location can be revealed. Thefeasibility of delamination detection with the proposed technique is demonstrated.

An optical fiber-based technique for continuous-level sensing

An optical fiber-based technique for continuous-level sensing