Quantum-dot mode-locked lasers have advantages such as high temperature stability, large optical bandwidth, and low power consumption, which make them ideal optical comb sources, especially for wavelength-division multiplexing (WDM) telecommunications, and optical I/O applications. In this work, we demonstrate an O-band quantum dot colliding pulse mode-locked laser (QD-CPML) to generate optical frequency combs with 200 GHz spacing with maximum channels of 12 within 3 dB optical bandwidth. To achieve the high output power of individual comb lines, four channel conditions are implemented at central wavelength of 1310 nm for WDM transmission experiments. Each channel exhibits more than 10 dBm output power with 200 Gb/s PAM-4 and 270 Gb/s PAM-8 modulation capability via thin-film LiNbO3 Mach–Zehnder interferometer modulator without the requirement of any optical amplifications. This high-order QD-CPML is an ideal comb source for power-efficient optical interconnects and large bandwidth optical data transmission.