Opposite Pattern Research Articles

Emotional vocalizations alter behaviors and neurochemical release into the amygdala

The basolateral amygdala (BLA), a brain center of emotional expression, contributes to acoustic communication by first interpreting the meaning of social sounds in the context of the listener’s internal state, then organizing the appropriate behavioral responses. We propose that modulatory neurochemicals such as acetylcholine (ACh) and dopamine (DA) provide internal-state signals to the BLA while an animal listens to social vocalizations. We tested this in a vocal playback experiment utilizing highly affective vocal sequences associated with either mating or restraint, then sampled and analyzed fluids within the BLA for a broad range of neurochemicals and observed behavioral responses of adult male and female mice. In male mice, playback of restraint vocalizations increased ACh release and usually decreased DA release, while playback of mating sequences evoked the opposite neurochemical release patterns. In non-estrus female mice, patterns of ACh and DA release with mating playback were similar to males. Estrus females, however, showed increased ACh, associated with vigilance, as well as increased DA, associated with reward-seeking. Experimental groups that showed increased ACh release also showed the largest increases in an aversive behavior. These neurochemical release patterns and several behavioral responses depended on a single prior experience with the mating and restraint behaviors. Our results support a model in which ACh and DA provide contextual information to sound analyzing BLA neurons that modulate their output to downstream brain regions controlling behavioral responses to social vocalizations.

Emotional vocalizations alter behaviors and neurochemical release into the amygdala.

The basolateral amygdala (BLA), a brain center of emotional expression, contributes to acoustic communication by first interpreting the meaning of social sounds in the context of the listener's internal state, then organizing the appropriate behavioral responses. We propose that modulatory neurochemicals such as acetylcholine (ACh) and dopamine (DA) provide internal-state signals to the BLA while an animal listens to social vocalizations. We tested this in a vocal playback experiment utilizing highly affective vocal sequences associated with either mating or restraint, then sampled and analyzed fluids within the BLA for a broad range of neurochemicals and observed behavioral responses of adult male and female mice. In male mice, playback of restraint vocalizations increased ACh release and usually decreased DA release, while playback of mating sequences evoked the opposite neurochemical release patterns. In non-estrus female mice, patterns of ACh and DA release with mating playback were similar to males. Estrus females, however, showed increased ACh, associated with vigilance, as well as increased DA, associated with reward-seeking. Experimental groups that showed increased ACh release also showed the largest increases in an aversive behavior. These neurochemical release patterns and several behavioral responses depended on a single prior experience with the mating and restraint behaviors. Our results support a model in which ACh and DA provide contextual information to sound analyzing BLA neurons that modulate their output to downstream brain regions controlling behavioral responses to social vocalizations.

Continental scale dietary patterns in a New World raptor using web-sourced photographs.

Dietary studies are essential to better understand raptor ecology and resource requirements through time and space, informing species habitat use, interspecific interactions and demographic rates. Methods used to collect data on raptor diets can constrain how dietary analyses can be interpreted. Traditional approaches to study raptor diets, such as analysis of pellets or prey remains, often provide dietary data at the local population level and tend to be restricted to pairs during the breeding season. The increasing use of citizen science data has the potential to provide dietary inferences at larger spatial, demographic and temporal scales. Using web-sourced photography, we explore continental-scale demographic and latitudinal dietary patterns between adult and non-adult Crested Caracaras (Caracara plancus), throughout the species' range across the Americas. We analysed 1,555 photographs of caracaras feeding and found no age effects on the probabilities of different food groups being included in photographs. The probability of reptiles being included in photographs of caracaras from the northern population was significantly higher than those from the southern population, with the opposite pattern for birds. There were significant latitudinal effects with the probabilities of fishes and invertebrates in the diet of northern caracaras increasing towards the equator. Contrastingly, the probability of mammals in the diet increased away from the equator for both populations. Assuming the focal species is well-sampled, web-sourced photography can improve our understanding of raptor diets at large-scales and complements more traditional approaches. This approach is more accessible to raptor researchers without access to the field or expertise in physical prey identification techniques.

Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases

microRNA (miR)-146a emerges as a promising post-transcriptional regulator in various inflammatory diseases with different roles for the two isoforms miR-146a-5p and miR-146a-3p. The present study aimed to examine the dual role of miR-146a-5p and miR-146a 3p in the modulation of inflammation in human pulmonary epithelial and immune cells in vitro as well as their expression in patients with inflammatory lung diseases. Experimental inflammation in human A549, HL60, and THP1 via the NF-kB pathway resulted in the major upregulation of miR-146a-5p and miR-146a-3p expression, which was partly cell-specific. Modulation by transfection with miRNA mimics and inhibitors demonstrated an anti-inflammatory effect of miR-146a-5p and a pro-inflammatory effect of miR-146a-3p, respectively. A mutual interference between miR-146a-5p and miR-146a-3p was observed, with miR-146a-5p exerting a predominant influence. In vivo NGS analyses revealed an upregulation of miR-146a-3p in the blood of patients with cystic fibrosis and bronchiolitis obliterans, while miR-146a-5p levels were downregulated or unchanged compared to controls. The reverse pattern was observed in patients with SARS-CoV-2 infection. In conclusion, miR-146a-5p and miR-146a-3p are two distinct but interconnected miRNA isoforms with opposing functions in inflammation regulation. Understanding their interaction provides important insights into the progression and persistence of inflammatory lung diseases and might provide potential therapeutic options.

Sex Dependence in Control of Renal Haemodynamics and Excretion in Streptozotocin Diabetic Rats—Role of Adenosine System and Nitric Oxide

Recently, we compared an interplay of the adenosine system and nitric oxide (NO) in the regulation of renal function between male normoglycaemic (NG) and streptozotocin-induced diabetic rats (DM). Considering the between-sex functional differences, e.g., in the NO status, we present similar studies performed in female rats. We examined if the theophylline effects (non-selective adenosine antagonist) in NG and DM females with or without active NO synthases differed from the earlier findings. In anaesthetised female Sprague Dawley rats, both NG and DM, untreated or after NO synthesis blockade with L-NAME, theophylline effects, on blood pressure, renal hemodynamics and excretion, and renal tissue NO were investigated. Renal artery blood flow (Transonic probe), cortical, outer-, and inner-medullary flows (laser-Doppler technique), and renal tissue NO signal (selective electrode) were measured. In contrast to males, in female NG and DM rats, theophylline induced renal vasodilation. In NO-deficient females, theophylline induced comparable renal vasodilatation, confirming the vasoconstrictor influence of the renal adenosine. In NG and DM females with intact NO synthesis, adenosine inhibition diminished kidney tissue NO, contrasting with an increase reported in males. Lowered baseline renal excretion in DM females suggested stimulation of renal tubular reabsorption due to the prevalence of antinatriuretic over natriuretic tubular action of adenosine receptors. An opposite inter-receptor balance pattern emerged previously from male studies. The study exposed between-sex functional differences in the interrelation of adenosine and NO in rats with normoglycaemia and streptozotocin diabetes. The findings also suggest that in diabetes mellitus, the abundance of individual receptor types can distinctly differ between females and males.

Exploring cross-linguistic differences in parental input and their associations with child expressive language in ASD: Bulgarian versus English comparison.

Parental input plays a central role in typical language acquisition and development. In autism spectrum disorder (ASD), characterized by social communicative and language difficulties, parental input presents an important avenue for investigation as a target for intervention. A rich body of literature has identified which aspects of grammatical complexity and lexical diversity are most associated with child language ability in both typical development and autism. Yet, the majority of these studies are conducted with English-speaking children, thus potentially overlooking nuances in parental input derived from cross-linguistic variation. To examine the differences in verbal parental input to Bulgarian- and English-speaking children with ASD. To examine whether aspects of verbal parental input found to be concurrent predictors of English-speaking children's expressive language ability are also predictors of the expressive language of Bulgarian-speaking children with ASD. We compared parental input to Bulgarian-speaking (N=37; 2;7-9;10 years) and English-speaking (N=37; 1;8-4;9 years) children with ASD matched on expressive language. Parent-child interactions were collected during free play with developmentally appropriate toys. These interactions were transcribed, and key measures of parental input were extracted. English-speaking parents produced more word tokens and word types than Bulgarian-speaking parents. However, Bulgarian parents produced more verbs in relation to nouns and used more statements and exclamations but asked fewer questions than English-speaking parents. In addition, child age and parents' use of questions were significant concurrent predictors of child expressive vocabulary. This is one of the first studies to conduct a cross-linguistic comparison of parental input in ASD. The differences found emphasize the need to further study parental input to Bulgarian children and adapt naturalistic parent-mediated interventions to the local language and its specific characteristics. What is already known on the subject A rich body of literature has identified the specific aspects of grammatical complexity, lexical diversity, and question-asking that are concurrently and longitudinally associated with the language ability of children with typical development and of children with ASD. Yet, the majority of these studies are conducted with English-speaking children. What this paper adds to the existing knowledge The present study finds that there are specific differences in verbal parental input to Bulgarian- and English-speaking children with autism in terms of lexical composition and question-asking. Bulgarian parents used more verbs than nouns, and the opposite pattern was found for English-speaking parents. In addition, Bulgarian parents asked fewer questions but used more statements and exclamations. Nevertheless, parental question use was significantly correlated with children's language ability across both groups, suggesting that question-asking should be further examined as a potential target for parent-mediated language interventions for Bulgarian children with autism. What are the potential or actual clinical implications of this work? Most language and social communication interventions for autism are designed and piloted with English-speaking children. These interventions are often simply translated and used in different countries, with different populations and in different contexts. However, considering that one of the defining characteristics of autism is language difficulty, more studies should examine (1) how these language difficulties manifest in languages other than English, and (2) what characterizes verbal parental input in these other contexts. Such research investigations should inform future language and social communication interventions. The present study emphasizes the cross-linguistic differences between Bulgarian- and English-speaking parents' verbal input to their children with autism.

Biomethanisation of sewage sludge: Sonication pretreatment and monitoring of microbial communities

The improvement of mesophilic biomethanisation of recalcitrant sewage sludge derived from urban wastewater treatment through the application of a sonication pretreatment was evaluated in parallel in two pilot-scale anaerobic digesters (two biological replicates: reactors RA and RB). The valorisation process was monitored through a novel and holistic approach that related the biomethanisation yield, and its main batch operational parameters, with the abundance of archaeal and bacterial communities in the anaerobic inocula. Sonication allowed achieving a methane yield coefficient derived from sewage sludge of 240 ± 20 mLSTPCH4/g VS (volatile solids) at the load range of 0.8–4.0 g VS/L in both reactors. The process was more stable in reactor B, with a wider range of loads being allowed (up to 5.29 g VS/L). Monitoring the presence of Archaea in the mixing liquor revealed a variation in their abundance throughout the process which was directly related to the availability of organic matter and pH. Advanced metagenomic analysis showed the phylogenetic and functional diversity of the complex microbiome involved. While Bacteria were widely distributed in 35 phyla, Archaea fitted in only two. Euryarchaeota was the majoritarian archaeal phylum (99.5 %) and its more abundant families are linked to methanogenic metabolism. Functional analysis revealed several relevant metabolic pathways that followed similar trends in both reactors. “Methane metabolism” clearly diminished at the end of the process in concordance with the exhaust of methane generation, while “ABC transporters” or “two-component systems”, involved in bacterial survival to changing environments, followed the opposite pattern. This integrated approach could help to increase the methanogenic valorisation of sewage sludge.

A dual RNA-seq analyses revealed dynamic arms race during the invasion of walnut by Colletotrichum gloeosporioides

BackgroundWalnut anthracnose caused by Colletotrichum gloeosporioides seriously endangers the yield and quality of walnut, and has now become a catastrophic disease in the walnut industry. Therefore, understanding both pathogen invasion mechanisms and host response processes is crucial to defense against C. gloeosporioides infection.ResultsHere, we investigated the mechanisms of interaction between walnut fruits (anthracnose-resistant F26 fruit bracts and anthracnose-susceptible F423 fruit bracts) and C. gloeosporioides at three infection time points (24hpi, 48hpi, and 72hpi) using a high-resolution time series dual transcriptomic analysis, characterizing the arms race between walnut and C. gloeosporioides. A total of 20,780 and 6670 differentially expressed genes (DEGs) were identified in walnut and C. gloeosporioides against 24hpi, respectively. Generous DEGs in walnut exhibited opposite expression patterns between F26 and F423, which indicated that different resistant materials exhibited different transcriptional responses to C. gloeosporioides during the infection process. KEGG functional enrichment analysis indicated that F26 displayed a broader response to C. gloeosporioides than F423. Meanwhile, the functional analysis of the C. gloeosporioides transcriptome was conducted and found that PHI, SignalP, CAZy, TCDB genes, the Fungal Zn (2)-Cys (6) binuclear cluster domain (PF00172.19) and the Cytochrome P450 (PF00067.23) were largely prominent in F26 fruit. These results suggested that C. gloeosporioides secreted some type of effector proteins in walnut fruit and appeared a different behavior based on the developmental stage of the walnut.ConclusionsOur present results shed light on the arms race process by which C. gloeosporioides attacked host and walnut against pathogen infection, laying the foundation for the green prevention of walnut anthracnose.

Haplotypes of ATP-Binding Cassette CaABCC6 in Chickpea from Kazakhstan Are Associated with Salinity Tolerance and Leaf Necrosis via Oxidative Stress

Salinity tolerance was studied in chickpea accessions from a germplasm collection and in cultivars from Kazakhstan. After NaCl treatment, significant differences were found between genotypes, which could be arranged into three groups. Those that performed poorest were found in group 1, comprising five ICC accessions with the lowest chlorophyll content, the highest leaf necrosis (LN), Na+ accumulation, malondialdehyde (MDA) content, and a low glutathione ratio GSH/GSSG. Two cultivars, Privo-1 and Tassay, representing group 2, were moderate in these traits, while the best performance was for group 3, containing two other cultivars, Krasnokutsky-123 and Looch, which were found to have mostly green plants and an exact opposite pattern of traits. Marker–trait association (MTA) between 6K DArT markers and four traits (LN, Na+, MDA, and GSH/GSSG) revealed the presence of four possible candidate genes in the chickpea genome that may be associated with the three groups. One gene, ATP-binding cassette, CaABCC6, was selected, and three haplotypes, A, D1, and D2, were identified in plants from the three groups. Two of the most salt-tolerant cultivars from group 3 were found to have haplotype D2 with a novel identified SNP. RT-qPCR analysis confirmed that this gene was strongly expressed after NaCl treatment in the parental- and breeding-line plants of haplotype D2. Mass spectrometry of seed proteins showed a higher accumulation of glutathione reductase and S-transferase, but not peroxidase, in the D2 haplotype. In conclusion, the CaABCC6 gene was hypothesized to be associated with a better response to oxidative stress via glutathione metabolism, while other candidate genes are likely involved in the control of chlorophyll content and Na+ accumulation.

Constructing lncRNA-miRNA-mRNA networks specific to individual cancer patients and finding prognostic biomarkers

BackgroundThe competitive endogenous RNA (ceRNA) hypothesis suggests that microRNAs (miRNAs) mediate a regulatory relation between long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) which share similar miRNA response elements (MREs) to bind to the same miRNA. Since the ceRNA hypothesis was proposed, several studies have been conducted to construct a network of lncRNAs, miRNAs and mRNAs in cancer. However, most cancer-related ceRNA networks are intended for representing a general relation of RNAs in cancer rather than for a patient-specific relation. Due to the heterogeneous nature of cancer, lncRNA-miRNA-mRNA interactions can vary in different patients.ResultsWe have developed a new method for constructing a ceRNA network of lncRNAs, miRNAs and mRNAs, which is specific to an individual cancer patient and for finding prognostic biomarkers consisting of lncRNA-miRNA-mRNA triplets. We tested our method on extensive data sets of three types of cancer (breast cancer, liver cancer, and lung cancer) and obtained potential prognostic lncRNA-miRNA-mRNA triplets for each type of cancer.ConclusionsAnalysis of expression patterns of the RNAs involved in the triplets and survival rates of cancer patients revealed several interesting findings. First, even for the same cancer type, prognostic lncRNA-miRNA-mRNA triplets can be different depending on whether lncRNA and mRNA show opposite or similar expression patterns. Second, prognostic lncRNA-miRNA-mRNA triplets are often more predictive of survival rates than RNA pairs or individual RNAs. Our approach will be useful for constructing patient-specific lncRNA-miRNA-mRNA networks and for finding prognostic biomarkers from the networks.

Constructing lncRNA-miRNA-mRNA networks specific to individual cancer patients and finding prognostic biomarkers

BackgroundThe competitive endogenous RNA (ceRNA) hypothesis suggests that microRNAs (miRNAs) mediate a regulatory relation between long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) which share similar miRNA response elements (MREs) to bind to the same miRNA. Since the ceRNA hypothesis was proposed, several studies have been conducted to construct a network of lncRNAs, miRNAs and mRNAs in cancer. However, most cancer-related ceRNA networks are intended for representing a general relation of RNAs in cancer rather than for a patient-specific relation. Due to the heterogeneous nature of cancer, lncRNA-miRNA-mRNA interactions can vary in different patients.ResultsWe have developed a new method for constructing a ceRNA network of lncRNAs, miRNAs and mRNAs, which is specific to an individual cancer patient and for finding prognostic biomarkers consisting of lncRNA-miRNA-mRNA triplets. We tested our method on extensive data sets of three types of cancer (breast cancer, liver cancer, and lung cancer) and obtained potential prognostic lncRNA-miRNA-mRNA triplets for each type of cancer.ConclusionsAnalysis of expression patterns of the RNAs involved in the triplets and survival rates of cancer patients revealed several interesting findings. First, even for the same cancer type, prognostic lncRNA-miRNA-mRNA triplets can be different depending on whether lncRNA and mRNA show opposite or similar expression patterns. Second, prognostic lncRNA-miRNA-mRNA triplets are often more predictive of survival rates than RNA pairs or individual RNAs. Our approach will be useful for constructing patient-specific lncRNA-miRNA-mRNA networks and for finding prognostic biomarkers from the networks.

Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat.

Winter plants rely on vernalization, a crucial process for adapting to cold conditions and ensuring successful reproduction. However, understanding the role of histone modifications in guiding the vernalization process in winter wheat remains limited. In this study, we investigated the transcriptome and chromatin dynamics in the shoot apex throughout the life cycle of winter wheat in the field. Two core histone modifications, H3K27me3 and H3K36me3, exhibited opposite patterns on the key vernalization gene VERNALIZATION1 (VRN1), correlating with its induction during cold exposure. Moreover, the H3K36me3 level remained high at VRN1 after cold exposure, which may maintain its active state. Mutations in FERTILIZATION-INDEPENDENT ENDOSPERM (TaFIE) and SET DOMAIN GROUP 8/EARLY FLOWERING IN SHORT DAYS (TaSDG8/TaEFS), components of the writer complex for H3K27me3 and H3K36me3, respectively, affected flowering time. Intriguingly, VRN1 lost its high expression after the cold exposure memory in the absence of H3K36me3. During embryo development, VRN1 was silenced with the removal of active histone modifications in both winter and spring wheat, with selective restoration of H3K27me3 in winter wheat. The mutant of Tafie-cr-87, a component of H3K27me3 "writer" complex, did not influence the silence of VRN1 during embryo development, but rather attenuated the cold exposure requirement of winter wheat. Integrating gene expression with H3K27me3 and H3K36me3 patterns identified potential regulators of flowering. This study unveils distinct roles of H3K27me3 and H3K36me3 in controlling vernalization response, maintenance, and resetting in winter wheat.

Corporate disclosure behavior during financial crises: Evidence from Korea

We examine corporate disclosure patterns according to changes in firm states during financial crises in Korea. Using panel data on Korean listed firms from 1995 to 2019, we first confirm that they transparently (opaquely) disclose information when the change in return on assets is positive (negative) during crises. Moreover, we check that these disclosure patterns increase debt financing but are ineffective for equity financing. Finally, for chaebols with internal capital markets, we find that internal capital receivers provide transparent (opaque) disclosure of negative (positive) changes in their states. By contrast, providers show the opposite patterns. (JEL G01, G30, M40)

Investigating the interplay between urban dynamics and environmental factors in Thimphu, Bhutan: a satellite remote sensing approach.

The ability of the land surface temperature (LST) and normalized difference vegetation index (NDVI) to examine land surface change is regarded as an important climate variable. However, no significant systematic examination of urbanization concerning environmental variables has been undertaken in the narrow valley of Thimphu, Bhutan. Therefore, this study investigated the impact of land use/land cover (LULC) dynamics on LST, NDVI, and elevation, using Moderate Resolution Imaging Spectroradiometer (MODIS) data collected in Thimphu, Bhutan, from 2000 to 2020. The results showed that LSTs varied substantially among different land use types, with the highest occurring in built-up areas and the lowest occurring in forests. There was a strong negative linear correlation between the LST and NDVI in built-up areas, indicating the impact of anthropogenic activities. Moreover, elevation had a noticeable effect on the LST and NDVI, which exhibited very strong opposite patterns at lower elevations. In summary, LULC dynamics significantly influence LST and NDVI, highlighting the importance of understanding spatiotemporal patterns and their effects on ecological processes for effective land management and environmental conservation. Moreover, this study also demonstrated the applicability of relatively low-cost, moderate spatial resolution satellite imagery for examining the impact of urban development on the urban environment in Thimphu city.

Dissociating Semantic Integration and Inhibitory Control in the Remote Associates Test: A tDCS-EEG Study

ABSTRACT Neuromodulation was utilized here to investigate the distinct involvement of two recognized cortical hubs for semantic integration (the left anterior temporal lobe, lATL) and inhibitory control (the right dorsolateral prefrontal cortex, rDLPFC) in creative problem-solving. Participants were presented with a list of category-exemplar words, selectively recalled some of them, and then solved a set of RAT problems. Selective retrieval was introduced to trigger inhibitory control over competitors. Critically, some RAT problems could be solved with words from the previous phases of the experiment, including words that might be less accessible due to inhibition. Other problems, however, could only be solved with unpresented words. Experiment 1 showed that anodal tDCS over the lATL had a negative effect on the production of correct responses to baseline RAT problems, but not on those that required inhibited solutions. Experiment 2 produced the reverse pattern with cathodal tDCS over the rDLPFC. Resting-state EEG recordings were obtained before and after delivering tDCS, which also revealed specific tDCS-induced changes in frequency bands depending on the site of stimulation. Overall, these findings provide support for the involvement of semantic and control processes in creative problem solving that are linked to different brain networks.

Numerical and semi-analytical approaches for heat transfer analysis of ternary hybrid nanofluid flow: A comparative study

This study presents a meticulous investigation into the intricate dynamics of a Fe3O4(25%)–Al2O3(50%)–ZnO(25%)/water ternary hybrid nanofluid within the framework of the Darcy–Forchheimer model, inclusive of heat source and suction/injection phenomena on two stretching surfaces. The governing partial differential equations are transformed into ordinary differential equations by strategically applying similarity variables. These equations are then solved using the efficient analytical approach of the Homotopy Analysis Method (HAM) and numerically using the implicit finite difference-based Keller Box Method (KBM). We further compare the solutions obtained through HAM with the numerical results from the KBM approach. The inquiry discerns those pivotal parameters such as porosity (Pm) and magnetic (M) amplifying the velocity profile (g), while parameters including Reynolds number (Re), inertia coefficient (Fr), rotational (Ro), and stretching (α) manifest diminishing effects. Furthermore, the study reveals a direct correlation between temperature escalation and the amplification of Eckert number (Ec), magnetic (M), radiation (R), heat source (Q), and stretching (α) parameters. In the case of suction applied to the lower and upper surfaces, the velocity profiles f′ and g, decrease whereas for injection, the opposite trend is observed. When there is suction at the upper surface the temperature profile decreases, but for suction at the upper surface the same increases. However, when there is injection, a reverse pattern is observed. Compared to water, suspension of nanoparticles with a 5% volume fraction of spheres, cylinders, platelets, and blades achieves 13%, 23%, 27%, and 36% heat transfer rates, respectively. This research provides crucial insights into nanofluid flow and heat transfer, with significant implications for various technological applications.

Reduced actual vapor pressure exerts a significant influence on maize yield through vapor pressure deficit amid climate warming.

Understanding the impact of climate warming on crop yield and its associated mechanisms is paramount for ensuring food security. Here, we conduct a thorough analysis of the impact of vapor pressure deficit (VPD) on maize yield, leveraging a rich dataset comprising temporal and spatial observations spanning 40 years across 31 maize-growing locations in Northeast and North China. Our investigation extends to the influencing meteorological factors that drive changes in VPD during the maize growing phase. Regression analysis reveals a linear negative relationship between VPD and maize yield, demonstrating diverse spatiotemporal characteristics. Spatially, maize yield exhibits higher sensitivity to VPD in Northeast China (NEC), despite the higher VPD levels in North China Plain (NCP). The opposite patterns reveal that high VPD not invariably lead to detrimental yield impacts. Temporal analysis sheds light on an upward trend in VPD, with values of 0.05 and 0.02 kPa/10yr, accompanied by significant abrupt changes around 1996 in NEC and 2006 in NCP, respectively. These temporal shifts contribute to the heightened sensitivity of maize yield in both regions. Importantly, we emphasize the need to pay closer attention to the substantial the impact of actual vapor pressure on abrupt VPD changes during the maize growing phase, particularly in the context of ongoing climate warming.

Mammary fat globules as a source of mRNA to model alterations in the expression of some milk component genes during lactation in bovines

BackgroundThe milk's nutritional value is determined by its constituents, including fat, protein, carbohydrates, and minerals. The mammary gland's ability to produce milk is controlled by a complex network of genes.Thereby, the fat, protein, and lactose synthesis must be boost in milk to increase milk production efficiency. This can be accomplished by fusing genetic advancements with proper management practices. Therefore, this study aimed to investigate the association between the Lipoprotein lipase (LPL), kappa casein CSN3, and Glucose transporter 1 (GLUT1) genes expression levels and such milk components as fat, protein, and lactose in different dairy breeds during different stages of lactation.MethodsTo achieve such a purpose, 94 milk samples were collected (72 samples from 36 multiparous black-white and red-white Holstein–Friesian (HF) cows and 22 milk samples from 11 Egyptian buffaloes) during the early and peak lactation stages. The milk samples were utilized for milk analysis and genes expressions analyses using non- invasive approach in obtaining milk fat globules (MFGs) as a source of Ribonucleic acid (RNA).ResultsLPL and CSN3 genes expressions levels were found to be significantly higher in Egyptian buffalo than Holstein–Friesian (HF) cows as well as fat and protein percentages. On the other hand, GLUT1 gene expression level was shown to be significantly higher during peak lactation than early lactation. Moreover, lactose % showed a significant difference in peak lactation phase compared to early lactation phase. Also, fat and protein percentages were significantly higher in early lactation period than peak lactation period but lactose% showed the opposite pattern of Egyptian buffalo.ConclusionTotal RNA can be successfully obtained from MFGs. The results suggest that these genes play a role in glucose absorption and lactose synthesis in bovine mammary epithelial cells during lactation. Also, these results provide light on the differential expression of these genes among distinct Holstein–Friesian cow breeds and Egyptian buffalo subspecies throughout various lactation phases.

O-278 The vaginal and endometrial microbiome: do they tell the same story?

Abstract Study question Is the composition of the endometrial microbiome comparable to that of the vagina and can vaginal sampling provide sufficient information to characterize the uterine microbiome? Summary answer The compositions of the endometrial and vaginal microbiome are largely comparable and therefore, in most cases, vaginal sampling is sufficient to characterize the uterine microbiome. What is known already A dysbiotic endometrial microbiome (i.e., a shift from a predominance of beneficial bacteria to less favourable species) has been reported in association with adverse pregnancy outcomes in assisted reproductive treatment (ART). If dysbiosis is detected, antibiotics or probiotics can be administered to restore an optimal microbiome. However, characterization of the endometrial microbiome is challenging, involving invasive sampling, and technical difficulties due to the low microbial mass in the endometrium. In contrast, vaginal sampling is considered minimally invasive and microbial analysis is technically less complex. It is important to clarify whether the microbiomes of the endometrium and the vagina are comparable. Study design, size, duration A prospective multi-centre study including 60 ART patients was conducted from June 2022 to August 2023. During the luteal phase (on average day-19), paired vaginal swabs and endometrial biopsies were collected sequentially and stored in a preservation medium for further analysis. Patients and physicians were also asked to complete a questionnaire to identify any lifestyle or medical factors that may have differentially affected the microbial composition of the vagina and endometrium. Participants/materials, setting, methods A carefully validated qPCR methodology was used to accurately assess the presence and relative abundance of the four major lactobacillus species associated with eubiosis (crispatus, gasseri, iners, and jensenii) and the 15 most common bacterial species implicated in dysbiosis. Additionally, the relative abundance of microbial and human DNA was examined. Of note, endometrial analysis required additional tissue lysis. Furthermore, due to the low microbial DNA content in endometrial samples, preamplification was necessary prior to qPCR. Main results and the role of chance The proportion of microbial versus human DNA was approximately 90 times higher in vaginal samples compared to endometrial samples (p < 0.0001). However, the microbial compositions of both the endometrium and the vagina showed remarkable similarities. Lactobacillus species were predominant at both sites, accounting for an average of 86% and 89% of the bacterial population in the vagina and endometrium, respectively (p = 0.6). Of the dysbiotic species, Gardnerella vaginalis was the most common, accounting for 8% (vagina) and 7% (endometrium) of the total bacterial population (p = 1). There was no difference in the average number of distinct microbial species between the two niches (1.8 vs. 2.1; p = 0.3). Direct comparison of paired samples highlighted the high similarity of microbial compositions between both sites and showed strong correlations of species abundances in individual patients (L.crispatus: r = 0.9, L.iners: r = 0.9, L.gasseri: r = 1, L.jensenii: r = 0.9, G.vaginalis: r = 0.7; p < 0.0001/all species). However, the microbial composition differed in four patients (6.6%), with three having a dysbiotic vaginal profile (≥50% dysbiotic species) but a eubiotic endometrial profile (≥50% lactobacillus species) and one showing the reverse pattern. The medical conditions of two patients, including chronic endometritis and use of antibiotics and probiotics in the preceding cycle, may account for the observed differences. Limitations, reasons for caution The species analysed were carefully selected to ensure a comprehensive analysis. Nevertheless, the potential presence of unevaluated species could impact the results. While the included patients displayed diversity in their reproductive histories, it is important to acknowledge that the observed results may still be influenced by underlying causes of infertility. Wider implications of the findings The close similarity between the microbiomes at the two sites investigated suggests that minimally invasive vaginal sampling may be sufficient to characterise the endometrial microbiome. The ease of vaginal sampling and processing is expected to improve patient recruitment in microbiome research and facilitate easier application of clinical microbiome tests. Trial registration number not applicable

Probiotics relieve growth retardation and stress by upgrading immunity in Nile tilapia (Oreochromis niloticus) during high temperature events

Global warming is disastrous to aquatic animals and supplementation of probiotics might mitigate its adverse effects. Therefore, to mitigate the effects of high temperature on growth retardation, stress and immunity, multi-species probiotics consisting of Bacillus subtilis (5×109 cfu/ml), B. thuringiencis (4×109 cfu/ml), Lactobacillus plantarum (5.8×109 cfu/ml), and L. buchneri (6.5×109 cfu/ml) were added in rearing water (1.0ml/L) of Nile tilapia (Oreochromis niloticus). Fingerlings (1.08 ± 0.13g) were reared at normal (31ºC) and elevated (34ºC and 37ºC) temperatures with or without probiotics for 6 weeks in triplicates. At the end of the experimental period, weight gain (WG) and specific growth rate (SGR) decreased significantly at 37ºC in fish supplemented with or without probiotics relative to normal temperature (31ºC). Importantly, at the high temperature (37ºC), WG and SGR were improved in fish supplemented with probiotics in contrast to fish reared without probiotics. The physiological status was improved by increasing the hemoglobin level and number of red blood cells and lowering white blood cells and glucose levels in the probiotics-treated high-temperature group. Frequencies of cellular and nuclear abnormalities of erythrocytes were significantly lowered in probiotics-supplemented fish. Histological observation demonstrated that multi-probiotics mitigated the high-temperature stress through increasing mucosal fold fattening, goblet cells, and the size of lamina propria and enterocytes in the intestine. Fish under higher temperatures (34 and 37ºC) showed up-regulated expression of the stress-related heat shock protein 70 (hsp70) gene, while its expression was decreased after probiotics addition. In contrast, two antioxidant-related genes (superoxide dismutase; SOD) and catalase; CAT) showed opposite expression patterns. The expression of three immune response-related genes (tumor necrosis factor alpha; TNF-α, interleukin 1 beta; IL-1β, and interferon gamma; IFN-γ) down-regulated with increase of temperature, while their expressions were increased after probiotics addition. Therefore, addition of probiotics in aquatic environment improved hemato-biochemical properties, blood cell structure, and immunity which ultimately relieve the growth retardation and stress in extreme temperatures in Nile tilapia.