Oplismenus Undulatifolius Research Articles

Inhibition of p-65, COX-2, iNOS and Cytokine Inflammation Signaling Pathway by Purified isoorientin from Oplismenus undulatifolius on Pro-inflammatory Responses

Inhibition of p-65, COX-2, iNOS and Cytokine Inflammation Signaling Pathway by Purified isoorientin from Oplismenus undulatifolius on Pro-inflammatory Responses

Epizoochorous seed dispersal by two Phasianidae birds: Green pheasant (Phasianus versicolor) and Chinese bamboo partridge (Bambusicola thoracicus)

AbstractWhile diverse animal species have been implicated in epizoochorous seed dispersal, studies specifically focused on bird‐mediated seed dispersal are scarce. We evaluated the potential contribution of two terrestrial birds, green pheasant (Phasianus versicolor) and Chinese bamboo partridge (Bambusicola thoracicus), to epizoochorous seed dispersal using a dummy‐based method. Furthermore, we compared the number of seeds attached to birds and mammals using data from a previous study conducted using the same method, site, and season. The results revealed 435 seeds of four herbaceous species (Achyranthes bidentata, Hylodesmum podocarpum, Oplismenus undulatifolius, and Persicaria filiformis) attached to two bird dummies. All seeds found on birds possessed adhesive appendages such as hooks, bristles, or viscous substances. In addition, the number of attached seeds does not exhibit significant differences between birds and mammals, irrespective of the season or plant species. Our findings provide the first documented evidence of epizoochorous seed dispersal by Phasianidae birds, highlighting the potential redundancy and complementarity between birds and mammals in mediating epizoochory.

Understory specific species appearances indicate the soil improvement of low-function Platycladus orientalis forest in Beijing’s mountainous areas

How to assess the effect of ecosystem restoration is a trending topic with all the restoration projects and ecosystem function studies in recent years. Although many studies have used different indicators and methods to evaluate ecosystem restoration effects, it needs a lot of site works to acquire the data. Low-function Platycladus orientalis forest restoration program in Beijing mountainous area has been started since 2002, in this study, the understory species of 92 plots were investigated and recorded. We assumed that the effect of ecosystem restoration would improve site conditions, especially for the soil conditions which might lead to the appearance of new species. The relationship between understory species and eleven site condition factors was elucidated by means of canonical correspondence analysis (CCA), further, specific species were chosen to represent the improvement and possible growth areas of specific species was predicted by Maximum Entropy model. Results shown that Spiraea pubescens, Deutzia parviflora, Chrysanthemum chanetii, Themeda triandra, Oplismenus undulatifolius were the main understory species which were sensitive to environmental factors, and their distribution were related to soil factors closely. The species of Spiraea pubescens, Chrysanthemum chanetii, Oplismenus undulatifolius had strong indication to soil nutrient, the appearances would happen when the comprehensive index of soil nutrient was above 81,64 and 61 respectively. The appearance of Chrysanthemum chanetii and Oplismenus undulatifolius would indicate that the soil nutrient level was in or above the range of medium, and the appearance of Spiraea pubescens would mean that the soil nutrient level was in or above the range of high in low-function Platycladus orientalis forest of Beijing mountainous area. Our study indicates that the specific species could as an indicator to reflect or evaluate the ecosystem restoration effect.

Seed longevity and germination of the emerging invasive species wavyleaf basketgrass (Oplismenus undulatifolius) under varied light regimes

AbstractInvasive nonindigenous species pose a serious threat to native biodiversity and ecosystem functioning. Understanding how species’ performance varies under conditions in the current and invaded range can help to predict the dynamics of the invading species in its new environment. Plants with the ability to alter growth in response to variation in light conditions may be favored in landscapes that experience frequent disturbance, as these species may be able to exploit a wide range of niches. Seedbank persistence may also play a critical role in successful plant invasion, as extended seed viability may increase the chance of outlasting unfavorable conditions, maintain population genetic diversity, and allow reinvasions. This study investigated seed longevity and the effect of light intensity on germination of wavyleaf basketgrass [Oplismenus undulatifolius (Ard.) Roem. & Schult.], a newly established invasive species in U.S. mid-Atlantic forest understories. Oplismenus undulatifolius seeds were collected across 5 yr from the original site of introduction in Maryland, USA, and stored in standard lab conditions, then subjected to germination trials under four light conditions in a controlled growth chamber. Seeds remained viable for at least 9 yr, and light intensity did not significantly impact seed germination. Our study demonstrates the importance of evaluating environmental and temporal effects on germination traits, because the scope of surveillance in the field may need to be expanded based on new information about environmental tolerance. Long-term monitoring may also be necessary to effectively control invasive plant populations capable of forming a persistent seedbank.

Seed attachment by epizoochory depends on animal fur, body height, and plant phenology

Various animal species potentially disperse varying amounts of seeds through epizoochory (seed dispersal on animals). However, few studies have compared the contributions of wild animals to epizoochory. Here, we compared the number of seeds of three plants—Achyranthes bidentata, Oplismenus undulatifolius, and Persicaria filiformis—attached to six mammal species: Meles anakuma, Mustela itatsi, Paguma larvata, Procyon lotor, Nyctereutes procyonoides, and Vulpes vulpes. We also investigated the effects of animal fur traits and height overlaps between animal body parts and seed-set positions. To evaluate the phenological effects, we conducted surveys in October and December. The number of seeds attached to the animal dummies differed among the mammal species and was significantly influenced by hair length and height overlap. Seed-set height changed overtime as the plants withered, which introduced seasonal variation in the disperser species. We conclude that epizoochorous seeds can be dispersed by various medium-sized mammals during their long period of availability. The number of seeds dispersed depends on the hair length, the height of the animal and, the seed-set position, and season.

Forage Plants of Gaur (<i>Bos gaurus</i> H. Smith, 1827) in Tropical Forest of Southern India

Forage plant species of Gaur ( Bos gaurus ) were studied by direct observation using focal sampling (90 hours) about 30 hours of sampling in three major habitats (Tropical dry deciduous forest, Moist deciduous forest and Thorn forest) in Mudumalai Tiger Reserve, Southern India from Jan-2003 to Aug-2004, also studied by feeding trail plots (924 1m2 plots and 495 5m2 plots) sampled in all the habitats. Gaur was observed to forage on 155 species of plants belonging to 38 families under 23 orders. Among different plant, physiognomic types trees and shrubs constituted a greater proportion by orders Rosales and Malvales. Herbs constituted 32% of the overall plant selected and consisted of 49 species in 17 orders and dominated by Asterales. The plant families such as Poaceae, Fabaceae, Asteraceae and Malvaceae collectively formed the major food species. Among the different species 29 grass, 9 herb species, 9 shrubs and 11 tree species were having more than one preference value. Gaur prefers to forage on Cenchrus purpureus, Axonopus compressus, Sprobolus diander, Themeda triandra, Oplismenus undulatifolius among grasses and Asystasia chelonoides and Sida cordifolia among herbs. The forage plant species of gaur was compared and discussed with other habitats of gaur distributional range.

Revision of the genus Aciculosporium (Clavicipitaceae) with a description of a new species on wavyleaf basketgrass, and proline-containing cyclic dipeptide production by A. take.

The genus Aciculosporium (Clavicipitaceae, Hypocreales, Ascomycota)was established in 1908 for A. take , which is the causal fungus of witches' broom of bamboo. Although the original description was valid at that time, a type specimen for A. take has not been designated. To standardize the use of this genus and species name, a neotypification and reference specimen of A. take are proposed. Multilocus phylogenetic analyses based on DNA sequences from 28S rDNA, TEF, Tub2, Mcm7, and RPB2 revealed that A.sasicola is from a different lineage to A. take, and other specimens from wavyleaf basket grass (Oplismenus undulatifolius) represent a distinct species proposed here as Aciculosporium oplismeni sp. nov. Chemical analysis using mass spectrometry and nuclear magnetic resonance spectroscopy showed that A. take produces four proline-containing cyclic dipeptides, which are moieties of ergot alkaloids. However, ergot alkaloids, lolines, peramine, indole-diterpenes, and lolitrem were not detected in the culture solvent. This study offers clarification of the lineage and morphology of this genus.

Elevation of anti-oxidative activity and inhibitory activities against tyrosinase, elastase, collagenase and hyaluronidase ofOplismenus undulatifoliusby elicitor treatment

본 연구에서는 생리활성물질유도제인 elicitor를 처리한 주름조개풀의 열수, 에탄올 추출물들의 항산화 효과, tyrosinase, elastase, collagenase 및 HAase 등의 미용관련 생리활성의 변화를 살펴보았다. Elicitor 처리한 주름조개풀 추출물의 DPPH 및 PF 등의 항산화 효과는 elicitor 비처리구인 대조구에 비해 elicitor 처리구가 농도의존적으로 더 높은 항산화 활성을 나타내었다. Elicitor 처리한 주름조개풀 추출물의 미용활성은 elastase 및 collagenase 억제 효과에 의한 주름개선효과와 tyrosinase 저해에 의한 미백효과가 비처리구인 대조구에 비해 상대적으로 매우 높은 효소 억제 효과를 나타내어 elicitor 처리가 주름조개풀추출물의 미백 및 주름개선 효과의 증대에 크게 영향을 미치는 것으로 확인되었으며, 농도의존적인 양상을 나타내었다. 또한 HAase 억제에 의해 유래되는 염증 억제 효과도 비처리구인 대조구에 비해 에탄올 추출물에서 우수한 염증 억제 효과를 나타내었다. Elicitor 처리에 의해 대조구에 비해 처리구에서 생리활성 효소에 대한 억제율이 큰 폭으로 상승하는 효과를 나타내어, elicitor 처리가 유용물질의 산업화라는 전제를 충족시키기 위한 biomass 기법으로 매우 적당하다는 것을 시사해주었다. 따라서 주름조개풀 노지재배 시에 본 연구에서 사용한 elicitor를 처리하면 수확되는 주름조개풀의 단위 무게 당 유용물질의 생산량을 높일 수 있고, 생리할성도 증대시킬 수가 있어 산업화를 위한 고기능성 원료생산에 효율적인 재배기법으로 활용할 수 있을 것이라 판단되었다.

Temperature, topography, soil characteristics, and NDVI drive habitat preferences of a shade-tolerant invasive grass.

AimDespite the large literature documenting the negative effects of invasive grasses, we lack an understanding of the drivers of their habitat suitability, especially for shade‐tolerant species that do not respond positively to canopy disturbance. We aimed to understand the environmental niche and potential spatial distribution of a relatively new invasive species, wavyleaf basketgrass (Oplismenus undulatifolius (Ard.) Roem. & Schult, WLBG) by leveraging data available at two different spatial scales.LocationMid‐Atlantic region of the United States.MethodsMaximum entropy modeling (Maxent) was used to predict the habitat suitability of WLBG at the regional scale and the landscape scale. Following variable evaluation, model calibration, and model evaluation, final models were created using 1,000 replicates and projected to each study area.ResultsAt the regional scale, our best models show that suitability for WLBG was driven by relatively high annual mean temperatures, low temperature seasonality and monthly range, low slope, and high cumulative Normalized Difference Vegetation Index (NDVI). At the landscape scale, suitability was highest near roads and streams, far from trails, at low elevations, in sandy, moist soil, and in areas with high NDVI.Main ConclusionsWe found that invasion potential of this relatively new invader appears high in productive, mesic habitats at low slope and elevations. At the regional scale, our model predicted areas of suitable habitat far outside areas where WLBG has been reported, including large portions of Virginia and West Virginia, suggests serious potential for spread. However, large portions of this area carry a high extrapolation risk and should therefore be interpreted with caution. In contrast, at the landscape level, the suitability of WLBG is largely restricted to areas near current presence points, suggesting that the expansion risk of this species within Shenandoah National Park is somewhat limited.

Evaluating the efficacy of removal treatments on wavyleaf basketgrass (Oplismenus undulatifolius)

AbstractWith the spread of a new invasive plant species, it is vital to determine the effectiveness of removal strategies as well as their advantages and disadvantages before attempting widespread removal. While thousands of dollars have been spent to curtail the spread of wavyleaf basketgrass [Oplismenus undulatifolius (Ard.) P. Beauv.], a relatively new invasive species, the lack of a cohesive management plan and funding has made controlling this species especially difficult. We assessed the efficacy of a variety of chemical control methods and hand weeding for this species and followed select methods over time. We also assessed the potential for ecosystem recovery following removal by measuring total and native species richness in response to treatments. Our pilot study revealed a wide breadth of responses to our eight herbicides, with fluazifop plus fenoxaprop, imazapic, quizalofop, and sulfometuron methyl being the least effective. In our follow-up experiments, hand weeding, glyphosate, and clethodim treatments were effective at reducing O. undulatifolius percent cover, density, and biomass, with an average reduction of at least 48% in the first year. However, we found substantial variation in the effectiveness of clethodim between our two experiments, which was likely driven by site differences. We also found that all three of these removal methods were effective at reducing the number of O. undulatifolius flowering stems and the height of those stems, which will likely reduce the spread of this species to new areas. Finally, we found that these methods have the potential to restore total and native species richness, but that glyphosate-treated plots did not fully recover until 2 yr after treatment.

The Weed Flora of Turkish Tea Plantations

This study was performed to find out the vascular weed taxa of tea ( Camellia sinensis (L.) Kuntze) plantations in Turkey and determine the critical control periods of the taxa which are negatively affect both of crop quality and yield. These taxa were determined with the studies and observations of authors both in tea plantations and local herbarium (KATO). A total of 114 native and naturalized/cultivated vascular plant taxa were found out as weeds in tea plantations. The most noxious weed, which were unintentionaly added to harvested shoots and negatively affected the tea quality, were underlined. The most commonly growing taxa in tea plantations in the region were: Pteridium aqulinum (L.) Kuhn., Smilax excelsa L., Alnus glutinosa (L.) Gaertn. subsp. barbata (C.A. Mey.) Yalt., Crassocephalum crepidioides (Bentham) S. Moore, Calystegia silvatica (Kit.) Griseb, Commelina communis L. , Microstegium vimineum (Trin.) A. Camus. , Oplismenus undulatifolius (Ard.) P. Beauw. and Rubus spp. A high number of the observed weed species are ecologically tolerated taxa which are naturalized in the region. Furthermore, of these taxa 9 are climbers and 14 are woody plants. The critical periods of weed control in tea plantations were derived from both literature and our ecological/phenological observatins in tea plantations.

Effects of phenolics from Oplismenus undulatifolius in α-MSH-stimulated B16F10 melanoma cells

Effects of phenolics from <i>Oplismenus undulatifolius</i> in α-MSH-stimulated B16F10 melanoma cells

Interspecific competitive potential of wavyleaf basketgrass (Oplismenus undulatifolius), a recent introduction to the mid-Atlantic United States

AbstractUnderstanding the mechanisms by which an invasive plant species is able to colonize and successfully expand into native plant communities can help in estimating the potential threat posed by a new invader and predict impacts on community diversity, structure, and function. Wavyleaf basketgrass [Oplismenus undulatifolius (Ard.) P. Beauv.] is a perennial, shade-tolerant grass species that has been recently introduced to the mid-Atlantic United States. Areas invaded by O. undulatifolius typically have low species richness, but it is unknown whether O. undulatifoius actively outcompetes other species or simply thrives primarily in species-poor habitats. This study used a greenhouse experiment to quantify interspecific competition in shade and sun among seedlings of O. undulatifolius; Japanese stiltgrass [Microstegium vimineum (Trin.) A. Camus], an invasive annual grass common in the region; and a mix of three native perennial grass species commonly used in restoring areas invaded by M. vimineum. In this experiment, shade did not significantly affect growth or competitive ability. Interspecific competition irrespective of shade had a negative effect on growth of all species, but O. undulatifolius was affected to a much greater degree than either M. vimineum or the native grass mix. These results suggest that, at least under these conditions, O. undulatifolius is a weak interspecific competitor and may be capable of forming dense monotypic stands only in areas that already have low species diversity. In the mid-Atlantic region, postagricultural legacies and overabundant deer populations, which lead to depauperate understories, may be a major facilitator of O. undulatifolius invasion in forests.

Analysis of mesophyll conductance in five understory herbaceous species.

Mesophyll conductance (g m) has received over time much less attention than stomatal conductance (g s), although it affects leaf photosynthesis to about the same extent as stomatal conductance does. The objective of this study was to analyze the g m trend in five understory herbaceous species growing in a close-canopy forest in the north-west of Italy. In particular, three of analyzed species were monocots: Carex brizoides Lam., Carex pilosa Scop., and Oplismenus undulatifolius P. Beauv and the others dicots species: Circaea lutetiana L., and Pulmonaria officinalis Ced. The results showed, on one hand, the absence of correlation between g m and the considered environmental variables in the forest understory (i.e. air temperature, photosynthetic photon flux density and carbon dioxide concentration). Moreover, we carried out a principal component analysis considering all the analyzed morphological and physiological variables for the five species. The following correlation between the first component, related to the leaf mass per unit of leaf area and the leaf tissue density, and g m seem to suggest a key role of the leaf structural features in determining g m variations across the five species.

The Relationship Between Native Insects and an Invasive Grass (Oplismenus undulatifolius) in the Mid-Atlantic United States

Understanding relationships between insects and invasive plant species in their introduced range is important for management of a new invader and prioritization of its control. This study investigates the insect communities associated with Oplismenus undulatifolius (Wavyleaf Basketgrass), an invasive grass in the mid-Atlantic US, and looks at the effect of herbivory on the species’ growth and reproduction. We surveyed aerial and ground-dwelling insect communities in areas with and without Wavyleaf Basketgrass and examined leaf samples from caged and uncaged patches of Wavyleaf Basketgrass for type and amount of insect damage. Insect richness, evenness, and diversity were similar between invaded and uninvaded areas. At the plot level, there was no difference in abundance of insects caught in pitfall traps, but there were more insects captured in sticky traps at the uninvaded area. Orthoptera in general and Rhaphidophoridae specifically, were indicators of uninvaded plots, along with Sciaridae in the Diptera and Scarabaeidae in the Coleoptera. Indicators of the invaded plots included Blattidae within the Blattodea and Staphylinidae and Carabidae within the Coleoptera. Leaf damage was minor; the 6 most heavily damaged leaves lost between 15% and 21% of their leaf area. Punctures, stippling, and mining were the most common types of leaf damage observed, and most leaves had fewer than 25 incidences of damage per leaf. There was no significant difference in leaf damage, plant biomass, or inflorescence production between caged and uncaged plots. Differences in insect community composition in invaded and uninvaded areas may be due to Wavyleaf Basketgrass itself or concomitant increases in plant cover and changes in microclimate. At least some insect groups are using Wavyleaf Basketgrass as habitat, which may be important in areas where excessive deer browse has removed most of the herbaceous layer.

Identification and characterization of new Muscodor endophytes from gramineous plants in Xishuangbanna, China.

The endophytic fungi Muscodor spp. produce volatile organic compounds (VOCs) which can inhibit and even kill pathogenic fungi, bacteria, and nematodes. Nine endophytic fungal strains, isolated from the shoots of gramineous plants including Arthraxon hispidus, Eleusine indica, Oplismenus undulatifolius, and Oryza granulata, were identified as Muscodor through phylogenetic analysis of the internal transcribed spacer. Through an SPSS K‐means cluster analysis, the nine Muscodor strains were divided into four groups based on the antifungal activities of the VOCs produced by these fungi determined by a two‐section confrontation test. The first group contains the strains Y‐L‐54, W‐S‐41, Y‐S‐35, W‐T‐27, and Y‐L‐56, which showed the strongest activity. The second and third groups contain W‐S‐35 and Y‐L‐43, which showed stronger and moderate activity, respectively. The fourth group contains W‐S‐38 and N‐L‐7, which were the weakest in inhibiting the tested pathogens. Thirty‐five compounds and the relative amounts of VOCs were determined by SPME‐GC‐MS and comparison with the NIST14 mass spectrometry database and Agilent MassHunter qualitative and quantitative analyses. These 35 compounds were classified into two different categories: (a) the product of fatty acid degradation, and (b) the intermediate and final metabolite of the metabolic pathway with the precursor of mevalonic acid. SPSS clustering analysis showed that the chemical components of VOCs might be correlated with their bioactivity rather than their phylogenetic assignment and some of the identified compounds might be responsible for antifungal activity. In conclusion, new Muscodor endophytes were recorded in tropical gramineous plants and a number of strains showed remarkable bioactive properties. Therefore, they have important potential applications in the fields of plant disease control.

Sesame phyllody disease-a potential emerging threat to sesamecultivation in the north east region of India

Sesame phyllody disease associated with the presence of phytoplasmas is becoming a serious problem to many sesame growing countries of the world including India. However no systematic study on the disease has been done to assess the disease incidence, characterization of the associated phytoplasma strains and effect on the yield in north eastern states of India. Therefore an extensive survey was conducted from 2015 to 2017 in seven north eastern states of India covering 52 districts to record the incidence of the disease and to perform phytoplasma associated molecular characterization. The infected plants showed symptoms of virescence, phyllody, floral proliferation, flattened stem, splitting of capsules and witches’ broom. The disease incidence varied from 2% to 29% in different locations. The highest disease incidence (29%) was observed in the Sonitpur district of Assam in the variety ST-1683. Sesame phyllody infection was found to reduce the number of pod and seed yield per plant from 72.82%-100% and 57.20%-100%, respectively. A total of 96 symptomatic sesame samples were collected and analysed on extracted DNA with polymerase chain reaction using phytoplasma specific primers, amplifying 16S rRNA and secA genes. PCR and sequencing analysis confirmed the presence of ‘Candidatus Phytoplasma asteris’-related strains in all the symptomatic samples. Pairwise sequence comparison and phylogenetic analyses of 21 strains on 16S rRNA gene and 9 strains on secA gene sequences confirmed the phytoplasma classification in the aster yellows groups. Among the 7 leafhopper species collected only Hishimonus phycitis tested positive for sesame phyllody phytoplasma presence. Seven species of weeds and wavy leaf basket grass (Oplismenus undulatifolius) and flax (Linum usitatissimum) in and around the infected sesame fields were also positive for phytoplasma presence indicating them as potential reservoir hosts of the phytoplasma associated with sesame phyllody disease in the north east region of India.

Characterization of polymorphic microsatellite loci for invasive wavyleaf basketgrass, Oplismenus undulatifolius (Poaceae).

Premise of the StudyNovel nuclear microsatellite markers were developed for the invasive plant Oplismenus undulatifolius (Poaceae) to facilitate studies of invasion dynamics for this recently introduced, high‐risk invasive species in North American mid‐Atlantic forests.Methods and ResultsSixteen polymorphic microsatellite loci were isolated and characterized from an Illumina paired‐end shotgun library of O. undulatifolius after initial assessment of 48 loci. When screened in three populations, these markers identified two to six alleles per locus. Observed heterozygosity ranged from 0.000 to 1.000. All loci were successfully amplified in the North American native congener O. hirtellus.ConclusionsWe provide the first nuclear genetic resources available for population genetic studies within the genus Oplismenus. These markers will serve as a critical tool for exploring patterns of gene flow and mechanisms of invasion of O. undulatifolius across its introduced range. These microsatellites should also be suitable for studies in other Oplismenus species.

Inhibitory activities on biological enzymes of extracts fromOplismenus undulatifolius

주름조개풀에 함유되어 있는 phenolic contents는 water, 70%ethanol을 용매로 하여 추출하였을 때 각각 7.7, 10.1 mg/g의 함량을 나타내었다. 추출물의 phenolic 농도를 50-200 μg/mL로 조절하여 항산화 효과를 측정한 결과, DPPH에서는 water, ethanol 추출물 50 μg/mL phenolics 농도에서 각각 78, 82%였고, ABTS radical decolorization는 100 μg/mL phenolics 농도에서 각각 92, 76%의 radical 소거능을 나타내었다. PF는 200 μg/mL phenolics 농도에서 water, ethanol 추출물에서 각각 1.51, 2.08 PF로 측정되었으며, TBARs값은 water, ethanol 추출물 50-μg/mL phenolics 농도에서 각각 84, 99%의 활성을 나타내었다. 기능성 식품 활성을 측정하는 α-Glucosidase 저해활성을 측정한 결과 ethanol 추출물 200 μg/mL phenolics 농도에서 44%의 억제력을 나타내었고, α-amylase 저해활성은 water 추출물 50-200 μg/mL phenolics 농도에서 37-88%의 효소 억제력을 나타내었다. 미용 식품 활성을 나타내는 tyrosinase 저해활성을 측정한 결과 ethanol 추출물에서 82%의 tyrosinase 저해효과를 나타내었다. 또한 elastase 저해활성을 측정한 결과 water, ethanol추출물에서 각각 4, 61%로 나타내었고, collagenase 저해활성을 측정한 결과 water, ethanol 추출물 200 μg/mL phenolics 농도에서 각각 39, 67%로 ethanol 추출물에서 높은 억제력을 나타내어 우수한 저해효과를 확인하였다. Astringent 효과를 측정한 결과 water, ethanol 추출물 200 μg/mL phenolic 농도에서 각각 13, 32%의 astringent 효과를 나타내었다. Hyaluronidae 저해활성을 측정한 결과 ethanol 추출물 200 μg/mL phenolic 농도에서 46%의 Hyaluronidae 저해효과를 나타내었다.

Variations of Leaf Cuticular Waxes Among C3 and C4 Gramineae Herbs

Modern C4 plants are commonly distributed in hot and dry environments whereas C3 plants predominate in cool and shade areas. At the outmost of plant surface, the deposition and chemical composition of cuticular waxes vary under different environmental conditions. However, whether such variation of cuticular wax is related to the distribution of C3 and C4 under different environmental conditions is still not clear. In this study, leaves of six C3 Gramineae herbs distributed in spring, Roegneriakamoji, Polypogonfugax, Poaannua, Avenafatua, Alopecurusaequalis, and Oplismenusundulatifolius, and four C4 and one C3 Gramineae herbs distributed in summer, Digitariasanguinalis, Eleusineindica, Setariaviridis, S. plicata, and O. undulatifolius, were sampled and analyzed for cuticular wax. Plates were the main epicuticular wax morphology in both C3 and C4 plants except S.plicata. The plates melted in C4 plants but not in C3 plants. The total cuticular wax amounts in C4 plants were significantly lower than those in C3 plants, except for O.undulatifolius. Primary alcohols were the most abundant compounds in C3 plants, whereas n-alkanes were relatively the most abundant compounds in C4 plants. C29 was the most abundant n-alkane in C3 plants except for O.undulatifolius, whereas the most abundant n-alkane was C31 or C33 in C4 plants. The average chain length (ACL) of n-alkanes was higher in C4 than in C3 plants, whereas the ACL of n-alkanoic acids was higher in C3 than C4 plants. The cluster analysis based on the distribution of n-alkanes clearly distinguished C3 and C4 plants into two groups, except for O.undulatifolius which was grouped with C4 plants. These results suggest that the variations of cuticular waxes among C3 and C4 Gramineae herbs are related to the distribution of C3 and C4 plants under different environmental conditions.