Noise, vibration, and harshness (NVH) issues induced by vibrating source to receivers are sensitive concerns in the automotive industry, significantly determining the product quality. Transfer path analysis (TPA) serves as a crucial tool in addressing these concerns. However, classical and component-based TPA methods necessitate the measurement of all transfer functions associated with the NVH phenomenon, causing high experimental costs; moreover, operational TPA (OTPA) involves matrix inversion processes that may result in the loss of important information. In this study, a neural network-based OTPA (NOTPA) method is proposed that eliminates the need for a matrix inversion process and utilizes only operational measurement. To derive the physical meaning of transmissibility obtained from the trained neural network model, the learning algorithm for NOTPA was developed, and appropriate learning rules were applied to the model. In the general neural network model, training was performed using real-valued parameters. However, the phase of the signal is crucial information in the theoretical formulation of TPA; thus, a complex-valued propagation algorithm was established. Furthermore, an appropriate activation function was chosen to derive the actual transfer mechanism from the trained complex-valued parameters. To experimentally verify the NOTPA method, a testbed resembling automotive vehicle structure was constructed. Estimation of sound pressure and noise contribution analysis were conducted using the NOTPA method and compared with conventional TPA methods. The proposed method demonstrated higher accuracy compared to the conventional OTPA method and successfully identified the main transfer path compared to the classical TPA. Furthermore, by comparing the estimated sound pressure according to the architecture of the model, the consistency of the proposed model was verified.