Open Tubular Capillary Column Research Articles

General Two-Step Method for the Fabrication of Covalent-Organic Framework-Bound Open-Tubular Capillary Columns for High-Resolution Gas Chromatography Separation of Isomers.

Covalent organic frameworks (COFs) are promising as stationary phases for gas chromatography (GC). The successful anchoring of COFs to the inner walls of the capillary with good uniformity is an important prerequisite to ensure the excellent separation performance of columns. However, current methods for the fabrication of COF-based capillary columns cannot always meet this requirement when faced with different COFs, which hampers the further development of COF-based GC stationary phases. Here, we show a general two-step method for the fabrication of COF-bound capillary column. The first step enables the formation of uniform amorphous polymer layer on the inner walls of capillary, while the second step allows the facile transformation of the amorphous polymer layer into a highly crystalline COF layer. COF-bound capillary columns with different framework structures were fabricated successfully by the developed two-step method. Impressively, the COF layers bound on the inner walls of these capillary columns showed good uniformity and high crystallinity. More importantly, as an example, the fabricated Tab-DHTA-bound capillary column showed good resolution (R > 1.5) and high column efficiency (700-39,663 plates m-1) for the tested isomers of ethylbenzene, xylene, dichlorobenzene, chlorotoluene, pinene, 1,3-dichloropropene, and propylbenzene with good precision (RSD, run-to-run, n = 5) (retention time, 0.2-0.6%; peak area, 0.5-1.1%; and peak height, 0.5-1.4%). In general, the fabricated Tab-DHTA-bound capillary column exhibited better performance for the separation isomers than commercial columns DB-5 and HP-FFAP. These results indicate that the two-step method is an efficient way to fabricate the COF-bound capillary column with excellent separation performance.

Chiral covalent organic framework-based open tubular capillary electrochromatography column for enantioseparation of selected amino acids and pesticides.

A novel chiral covalent organic framework (CCOF) was synthesized with an imine covalent organic framework TpBD (synthesized via Schiff-base reaction between phloroglucinol (Tp) and benzidine (BD)) modified using (1S)-(+)-10-camphorsulfonyl chloride as chiral ligand by chemical bonding method for the first time, and was characterized by X-ray diffraction, Fourier-transform infrared spectra, X-ray photoelectron spectroscopy, nitrogen adsorption/desorption, thermogravimetry analysis, and zeta-potential. The results revealed that the CCOF had good crystallinity, high specific surface area and good thermal stability. Then, the CCOF was employed as stationary phase in open-tubular capillary electrochromatography (OT-CEC) column (the CCOF-bonded OT-CEC column) for enantioseparation of 21 single chiral compounds (12 natural amino acids including acidic, neutral and basic, 9 pesticides including herbicides, insecticides and fungicides) and simultaneous enantioseparation of mixture amino acids and pesticides with similar structures or properties. Under the optimized CEC conditions, all the analytes reached the baseline separation with high resolutions of 1.67-25.93 and selectivity factors of 1.06-3.49 in 8min. Finally, the reproducibility and stability of the CCOF-bonded OT-CEC column were measured. Relative standard deviations (RSDs) of retention time and separation efficiency were 0.58-4.57% and 1.85-4.98%, and not obviously changed after 150 runs. These results demonstrate that COFs-modified OT-CEC explore a promising method to separate chiral compounds.

Preparation of β-cyclodextrin covalent organic framework-immobilized poly(glycidyl methacrylate) nanoparticle-coated open tubular capillary electrochromatography column for chiral separation.

A new enantioselective open-tubular capillary electrochromatography was developed employing poly(glycidyl methacrylate) nanoparticles/β-cyclodextrin covalent organic frameworks chemically immobilized on the inner wall of the capillary as a stationary phase. A pretreated silica-fused capillary reacted with 3-aminopropyl-trimethoxysilane followed by poly(glycidyl methacrylate) nanoparticles and β-cyclodextrin covalent organic frameworks through a ring-opening reaction. The resulting coating layer on the capillary was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The electroosmotic flow was studied to evaluate the variation of the immobilized columns. The chiral separation performance of the fabricated capillary columns was validated by the analysis of the four racemic proton pump inhibitors including lansoprazole, pantoprazole, tenatoprazole, and omeprazole. The influences of bonding concentration, bonding time, bonding temperature, buffer type and concentration, buffer pH, and applied voltage on the enantioseparation of four proton pump inhibitors were investigated. Good enantioseparation efficiencies were achieved for all enantiomers. In the optimum conditions, the enantiomers of four proton pump inhibitors were fully resolved within 10min with high resolutions of 9.5-13.9. The column-to-column and inter- to intra-day repeatability of the fabricated capillary columns through relative standard deviation were found better than 9.54%, exhibiting satisfactory stability and repeatability of the fabricated capillary columns.

Preparation of open tubular capillary column covalently coated with polystyrene sulfonate with 4,4'-azobis (4-cyanopentanoyl chloride) as polymerization initiator for electrochromatographic separation of alkaloids, sulfonamides and peptides.

An open tubular capillary electrochromatography column covalently bonded with polystyrene sulphonate was prepared via in situ polymerization using functionalized azo-initiator 4,4'-Azobis(4-cyanopentanoyl chloride). Scanning electron, fluorescence and atomic force microscopy techniques showed the formation of a relatively rough layer of polymer. In addition, -CN and C = O stretching vibrations from infrared spectroscopy proved the successful immobilization of the azo-initiator through covalent bonding and X-rayPhotoelectronSpectroscopy confirmed the elemental composition of the formed polymer layer. The prepared column was found to be appropriate for small and medium-sized molecules separation. Compared to bare fused silica capillary column higher selectivity and resolution were obtained for the separation of alkaloids, sulfonamides and peptides as a result of the electrostatic and pi-pi stacking interactions between the small organic molecules and the coated column without compromising the electroosmotic flow mobility. Separation efficiency was also increased compared to the bare capillary for the separation of alkaloids (about 1.5 times). Moreover, intraday, inter day, intra batch and inter batch relative standard deviation values of retention time and peak area of peptides were within 2% and 10%, respectively, indicating a good repeatability of the column preparation procedure. The developed method for the covalent bonding of polymers through a functionalized azo-initiator could represent a promising stable method for the preparation of open tubular column. This article is protected by copyright. All rights reserved.

Practical aspects of preparation, optimization, and evaluation of poly(styrene-divinylbenzene)porous-layer open tubular (PLOT) columns for capillary liquid chromatography

Using open tubular (OT) capillary columns in the nano and micro liquid chromatography scales has several advantages over conventional large-bore packed columns. Among the open tubular columns, those of the PLOT (porous layer open tubular) type are more suitable for liquid chromatography due to the porous character of the stationary phase that provides more significant interaction with the analytes. In addition, recent instrumental advances in capillaryLC coupled with mass spectrometry (MS) have opened up new avenues for developing and applying OT-LC-MS. This work discusses the possibility of applying PLOT columns in the capillary LC flow range (0.5–2 μL min−1) and describes the preparation, optimization, and evaluation of those columns. Poly(styrene-divinylbenzene) (PS-DVB) was used as the stationary phase, and the internal diameter of the columns discussed in this work was 10 and 25 μm. A comparison between these diameters is presented regarding fabrication and practical aspects of the operation. Additionally, for the 25 μm PLOT column, the gradient window and the flow rate effects on the system's performance were investigated. Flow rates of 1 μL min−1 and a gradient window of 60% (from 20 to 80% of acetonitrile) were the best conditions to work on this column in capillary LC conditions.

Determination of benzimidazoles in fruits by open-tubular capillary electrochromatography based on ionic liquids grafted covalent organic frameworks.

A novel capillary electrochromatography method has been developed for the simultaneous quantification of ten benzimidazole fungicides in fruits. Herein, covalent organic frameworks (COFs) and ionic liquids (ILs) were successfully introduced to prepare open-tubular capillary column to improve the loading capacity and separation performance. The parameters effecting the analytical performance including pH and concentration of running buffer, separation voltage and the addition of organic solvent were investigated systematically. Under the optimized conditions, the method allowed the baseline separation of ten benzimidazole fungicides, and showed a good linearity in the range of 3.5-200μgkg-1 with the detection limits between 1.0 and 2.8μgkg-1. The intraday and interday precisions for recoveries were lower than 7.9% and 12.2%, respectively. Intraday and interday precisions for their retention times were lower than 3.2% and 6.6%, respectively. Satisfactory recoveries for grape, pear and orange samples at two concentrations were obtained ranging from 85.0 to 95.9% with RSDs lower than 7.8%, demonstrating the potential applications of the open-tubular capillary electrochromatography method for trace benzimidazole fungicides analysis in fruits.

Homochiral iron-based γ-cyclodextrin metal-organic framework for stereoisomer separation in the open tubular capillary electrochromatography

Metal organic frameworks (MOFs), as a novel separation mediums, have in recent years attracted wide consideration in capillary electrochromatography (CEC). However, the instability of frameworks limited the application of the MOF-based chiral separation capillary. Herein, Iron-based γ-cyclodextrin metal-organic framework (Fe-CD-MOF) with mesoporous was developed as the chiral stationary phase in open tubular capillary electrochromatography column (OT-CEC) for the stereoisomer separation of fourteen chiral drugs and one chiral alcohol. The capillary column with MOF coating exhibited the best performance (resolution value: 17.07) for anisodamine in any MOF-based capillary column that has never been reported. Moreover, the effect of pH, buffer concentration, and methanol content has been investigated. The novel coated capillary was also applied for the quantitative analysis of a real sample. The Fe-CD-MOF coated capillary showed outstanding repeatability and stability, with the satisfactory relative standard deviations (RSDs) for intra-day, inter-day, and column-to-column. Finally, the enantioseparation mechanism of the Fe-CD-MOF coated capillary was evaluated by adsorption kinetic experiments. In summary, this work indicated the novel and renewable Fe-CD-MOF was a potential chiral stationary phase in CEC chiral separation.

Preparation and evaluation of ultra-long open-tubular capillary columns modified with zeolitic imidazolate framework-8 incorporated polymeric porous layer for liquid chromatography

An ultra-long (5 m) open tubular capillary liquid chromatographic column was prepared by incorporating Metal Organic Framework (MOF), zeolitic imidazolate framework-8 (ZIF-8), directly into polymer coating, which was synthesized by the copolymerization of 4-vinylbenzyl chloride and divinylbenzene, on the capillary inner surface. The prepared ZIF-8 incorporate polymeric open tubular capillary column (denoted as ZIF-8-p(VBC/DVB) OTCC) was evaluated with thiourea, alkylbenzenes and polycyclic aromatic hydrocarbons as probe molecules. The results showed that the ultra-long column achieved absolute column efficiency of 130,000 plates for thiourea, and the incorporation of ZIF-8 effectively improved the chromatography performance of the OTCC. Baseline separation of aromatic compounds and position isomers was achieved based on multiple interactions provided by the zeolitic imidazolate framework and polymer, including hydrophobic interaction, π-π stacking interaction and the coordination effect. The RSD values (run-to-run, day-to-day, column-to-column, n = 3) of retention time of phenylenediamine isomers and propylbenzene isomers were less than 0.7%, 1.2% and 4.0% respectively, suggesting excellent repeatability. Finally, the prepared ZIF-8-p(VBC/DVB) OTCC was applied to the separation of hydroxyacetophenone isomers with satisfied results.

A Novel Chiral Covalent Organic Framework in Open Tubular Capillary Electrochromatography Column for Rapid and Simultaneous Enantioseparation of Amino Acids and Pesticides

A Novel Chiral Covalent Organic Framework in Open Tubular Capillary Electrochromatography Column for Rapid and Simultaneous Enantioseparation of Amino Acids and Pesticides

Preparation and properties of GC capillary column with hypercrosslinked stationary phase.

For the first time, highly porous hypercrosslinked polystyrene layer was synthesized within a gas chromatography capillary column and successfully deposited onto the capillary walls generating porous layer open-tubular capillary column. Elaborated three steps synthetic procedure provides tightly bonded porous polymeric layer and ensues complete elimination of particle shedding and the needs for particle traps. Due to highly developed surface area, porous layer open-tubular column provides strong solute retention that is useful for the separation of various volatile solvents and light gas compounds including ethane, ethylene, ethyne. Aqueous injections will not harm the column.

Growth of two-layer copolymer as the stationary phase with very high separation efficiency for separating peptides in capillary electrochromatography.

Open tubular CEC (OT-CEC) column with a very high separation efficiency was prepared for peptides separation. A pretreated silica-fused capillary was reacted with 3-(methacryloxy) propyltrimethoxysilane followed by vinylbenzyl chloride and divinylbenzene to produce first thin monolithic monolayer. The second copolymer layer was formed on thin monolithic monolayer of the capillary by reversible addition-fragmentation transfer polymerization of N-phenylacrylamide and styrene. The key parameters including buffer pH value and organic modifier were systematically evaluated to provide the optimal chromatographic condition. The resultant OT-CEC columns were validated by separating a synthetic mixture of peptides and cytochrome C tryptic digest in capillary electrochromatography. The number of theoretical plates as high as 2.4 million per column was achieved for synthetic mixture peptides. In addition, the fabricated OT-CEC column also resolved more than 18 high-efficiency digestion peptides from a mixture containing tryptic digest of cytochrome C. The column to column and inter- to intraday repeatabilities of OT-CEC column through RSD% were found better than 3.0%, exhibiting satisfactory stability and repeatability of the two-layer deposited OT-CEC column. The results reveal that the open tubular capillary column modified with two-layer copolymer shows the great prospect for the separation of proteins in capillary electrochromatography.

Preparation of a polydopamine/β-cyclodextrin coated open tubular capillary electrochromatography column and application for enantioseparation of five proton pump inhibitors.

An open tubular capillary electrochromatography column was prepared by immobilizing β-cyclodextrin on the inner wall of pretreated capillary via noncovalent adsorption of polydopamine. The resulting coating layer on the capillary was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Electroosmotic flow was studied to evaluate the variation of the immobilized columns. The prepared columns showed good chiral separation performance toward five proton pump inhibitors including lansoprazole, pantoprazole, tenatoprazole, rabeprazole, and omeprazole. The influences of β-cyclodextrin concentration, coating time, buffer pH, buffer concentration, and applied voltage on separation were investigated. In the optimum conditions, the enantiomers of five analytes were fully resolved within 15min with high resolutions of 4.57 to 8.13. The method was extensively validated in terms of accuracy, precision, and linearity and proved to be robust. The relative standard deviation values for migration times and peak areas of the analytes representing intraday and interday were less than 1.9 and 3.6%, respectively. Further, the polydopamine/β-cyclodextrin coated capillary column could be successively used over 100 runs without showing significant decrease in the separation efficiency.

Open tubular capillary column immobilized with sulfobutylether-β-cyclodextrin for chiral separation in capillary electrochromatography.

A novel chiral open tubular capillary column was fabricated with sulfobutylether-β-cyclodextrin and glycidyl methacrylate for enantioseparation in capillary electrochromatography. First, the pretreated silica-fused capillary was treated with 3-trimethoxysilyl propyl methacrylate to attach double bond ligand onto the surface. A copolymer layer was formed on the surface of capillary using glycidyl methacrylate and ethylene dimethacrylate by in situ one-pot polymerization. Sulfobutylether-β-cyclodextrin was encapsulated inside the copolymerized layer. The morphology of the developed column was characterized by field emission scanning electron microscopy. The effect of organic percentage and pH value of the mobile phase on electroosmotic flow and resolution was also investigated. The performance of the fabricated column was validated by separation of amlodipine besilate, 2,3-diphenylpropionic acid, tropic acid, and pantoprazole enantiomers with good resolutions of 3.67, 4.82, 3.34, and 2.61, respectively. The repeatabilities of column-to-column and day-to-day through relative standard deviation were found better than 4%, exhibiting satisfactory repeatability of the developed column. The results reveal that open tubular capillary columns modified with β-cyclodextrin show a great prospect for enantioseparation of chiral drugs in capillary electrochromatography.

Preparation and Evaluation of Ultra-Long Open-Tubular Capillary Columns Modified with ZIF-8 Incorporated Polymeric Porous Layer for Liquid Chromatography

Preparation and Evaluation of Ultra-Long Open-Tubular Capillary Columns Modified with ZIF-8 Incorporated Polymeric Porous Layer for Liquid Chromatography

A porous layer open-tubular capillary column supported with pepsin and zeolitic imidazolate framework for enantioseparation of four basic drugs in capillary electrochromatography

New material zeolitic imidazolate framework-4, 5-imidazoledicarboxylic acid (ZIF-IMD) located on the pore surface of porous layer open-tubular (PLOT) column previously functionalized with N-(3-aminopropyl)-imidazole have been prepared via a layer-by-layer self-assembly strategy. This new ZIF-IMD coating hybrids are used as solid-phase carriers for chiral selector pepsin immobilization. The ZIF-IMD material was characterized by scanning electron microscopy, energy-dispersive spectroscopy, transmission electron microscope and X-ray diffraction. The synthesized pepsin@ZIF-IMD@POLT column achieved the baseline separation of hydroxychloroquine (HCQ), chloroquine (CHQ) and hydroxyzine (HXY) (the resolution of HCQ: 2.19; CHQ: 1.84; HXY: 1.53). Compared with the pepsin@PLOT column (without ZIF-IMD material), the chiral separation capability of the pepsin@ZIF-IMD@POLT column can be remarkably improved. Several key parameters including concentration of chiral selector, buffer pH, applied voltage and buffer concentration were systematically evaluated to provide the optimal enantioseparation condition. The relative standard deviations (RSDs) of intra-day, inter-day, column-to-column and inter-batch of migration time and Rs of the HCQ were evaluated in detail, respectively (RSD < 7.21%). Additionally, the potential mechanism of increased resolution was discussed in the article.

A novel one‐pot strategy of preparation of N‐phenylacrylamide‐styrene co‐polymer open tubular capillary column for peptides separation

An open tubular capillary column (100μm internal diameter and 120cm length) was first fabricated with N-phenylacrylamide and styrene copolymer layer on the inner surface by in situ one-pot strategy, while ethylene dimethacrylate was used as the cross-linker. The pretreated silica-fused capillary was reacted with 3-trimethoxysilyl propyl methacrylate to attach a double-bond ligand onto the inner surface of the capillary. Further, a thick and stabile copolymer layer was generated on the inner surface of the capillary by the novel method in situ one-pot reaction of styrene-N-phenylacrylamide. The effects of reaction temperature and composition of the polymerization mixture on the morphology and permeability of the copolymer were investigated. The separation performance of the fabricated polymer columns were validated by separation of five synthetic peptides. The excellent efficiency (188500 plates/m) of 100μm internal diameter open tubular column with the separation media prepared by mixed one-pot copolymerization has not been achieved via the isocratic elution mode. The column to column, intraday, and interday repeatabilities evaluated from the relative standard deviation were found better than 4%, exhibiting considerable repeatability of the column.

Identifying an unknown compound in flue gas of semiconductor industry – Forensics of a perfluorocarbon

This study investigated the source of a false positive signal in the measurement of total non-methane organic compounds (TNMOCs) by an on-line analyzer based on flame ionization detection (FID) in the flue gas released from a semiconductor fabrication plant. Since no release of volatile organic compounds (VOCs) into the waste gas stream in acid/base ventilation was assured by the plant authority, the positive detection of VOCs became a subject of dispute. In addition to the TNMOC analysis of 5 samples, the investigation used the method that coupled thermal desorption (TD) with gas chromatography mass spectrometry (GC/MS), dubbed TD-GC/MS, to identify the substance that produced the FID signals of TNMOCs. The waste gas was collected with sampling canisters and analyzed by in-laboratory TD-GC/MS. However, mass scanning from 45 to 250 m/z to remove interference from air matrix of O2, N2 and CO2 forbid detecting any ion fragments smaller than 45 m/z and, thus, led to poor match in mass (MS) library search. As a result, a highly retentive porous layer open tubular (PLOT) capillary column was employed to separate the unknown away from the air peak. The success of acquiring all key ion fragments of 31, 50, 69, and 131 m/z resulted in an excellent match with octafluorocyclobutane (C4F8) in the NIST database. A gas standard was then prepared and injected to confirm the identity of C4F8 by the identical mass spectrum and GC retention time. The concentrations of C4F8 found in the 5 flue gas samples varying from 3.32 to 6.21 ppmv were very close to the NMOC range of 3.48–6.62 ppmv as reported by the TNMOC analyzer, proving that the FID signals observed from TNMOC method were mostly produced from C4F8. Consequently, the method of TD-GC/MS would be an ideal method to pre-screen the presence of PFCs before a non-distinguishable TNMOC analyzer is applied to approximate the VOC level as part of the integrated effort to monitor VOC in flue gas.

Reactions

OPINION Reactions ShareShare onFacebookTwitterWechatLinked InRedditEmail C&EN, 2020, 98 (19), p 3May 18, 2020Cite this:C&EN 98, 19, 3AbstractLetters to the editor Fiber optics and gas chromatography As usual, Mitch Jacoby has written an article, “A Way Forward for Optical Fibers” (C&EN, March 16, 2020, page 28), that is worthy of study. Analytical chemists should also take note. Jacoby points out that after Charles K. Kao’s pioneering work with optical fibers, commercialization largely by telecom companies took off in the 1980s. Much before that time, a meeting took place in East Lansing, Michigan, in 1957 when Marcel Golay introduced the idea of open-tubular capillary gas chromatography columns. The pioneering work of Raymond Dandeneau and E. H. Zerenner (J. High Resolut. Chromatogr. Chromatogr. Commun. 1979, DOI: 10.1002/jhrc.1240020617) at Hewlett-Packard led to the fused silica wall-coated open-tubular (WCOT) gas chromatographic column that revolutionized the practice of gas chromatography. A nice look at how WCOT GC columns evolved using fused silica—the same as that used in fiber-optic communications—can be found inView: PDF | Full Text HTML

Carbon Nanotube (CNT) Stationary Phase for Micro Gas Chromatograph Columns

Introduction Microfabricated gas chromatography systems based upon MEMS fabrication technology are under development for portable chemical vapor analysis and chemical detection. In particular, Sandia National Laboratories has pioneered development of these miniature systems (1). Current methods for coating microfabricated gas chromatography columns are based upon deposition from a saturated mixture of siloxanes in a solvent, which are adapted from techniques developed for coating of open-tubular capillary columns (2). This static method is more successful with silica open tube columns which have a uniform cross-section area, however in a microfabricated column, a rectangular cross-section is produced in the channel. The square corners tend to have a thicker layer of material deposited using this process and hence these thicker layers provide a different retention time for the analytes which results in peak broadening and reduction in resolution and peak capacity of the column. Alternative stationary phases have been investigated (3), and using chemical vapor deposition (CVD) they can be grown with uniform thickness, for example carbon nanotubes (CNTs) (4) The CNTs have high surface area and are stable at high temperatures. Method A silicon substrate is used for the fabrication of a microfabricated spiral column, 6m in length using standard photolithography and deep reactive ion etching fabrication processes (5). An ultra-thin layer of iron catalyst is deposited by sputtering into the etched silicon channel. Using a Black Magic plasma enhanced CVD growth a layer of CNTs are formed thickness 0.5 to 1 micrometer. Figure 1 shows an SEM micrograph of the deposited CNTs layer which coats the sides of channel and base with uniform thickness. Next a layer of gold is deposited for eutectic bonding of the silicon lid in order to enclose the channel making the column. Finally high temperature annealing is carried out at 450 oC to increase bond strength. Results The column is mounted inside a commercial Agilent 6890 GC system oven with a short length of guard column, approximately 0.5m length, connect between the split injector and the flame ionization detector. A liquid injection of three compounds, hexane, octane and decane, volume of 0.02uL, was carried out with a 100:1 split and injector temperature of 275°C. The helium carrier gas flow rate was 0.1 ml/min. A typical chromatogram is shown in Figure 2. The McReynolds probes for polarity were also injected, and the Kovats indices were evaluated for comparison with OV-1 (Ohio Valley, OH) polymer stationary phase. Conclusions The use of CNT’s for a stationary phase may offer some advantages with micro-GC column fabrication because the quality of the layer can be inspected prior to bonding. We can observe the low polarity of the CNT stationary phase compared to the poly-siloxane However, it may be possible to chemically functionalize the surface for the CNTs for more polar compound separation and analysis.

Preparation of a thiols β-cyclodextrin/gold nanoparticles-coated open tubular column for capillary electrochromatography enantioseparations.

Inspired by the distinct chemical and physical properties of nanoparticles, here a novel open-tubular capillary electrochromatography column was prepared by electrostatic assembly of poly(diallydimethylammonium chloride) onto the inner surface of a fused-silica capillary, followed by self-adsorption of negatively charged SH-β-cyclodextrin/gold nanoparticles. The formation of the SH-β-cyclodextrin/gold nanoparticles coated capillary was confirmed and characterized by scanning electron microscopy and energy dispersive spectrometry. The results of scanning electron microscopy and energy dispersive spectrometry studies indicated that SH-β-cyclodextrin/gold nanoparticles were successfully coated on the inner wall of the capillary column. The performance of the SH-β-cyclodextrin/gold nanoparticles coated capillary was validated by the analysis of six pairs of chiral drugs, namely zopiclone, carvedilol, salbutamol, terbutaline sulfate, phenoxybenzamine hydrochloride, and ibuprofen. Satisfactory enantioseparation results were achieved, confirming the use of gold nanoparticles as the support could enhance the phase ratio of the open-tubular capillary column. Additionally, the stability and reproducibility of the SH-β-cyclodextrin/gold nanoparticles coated capillary column were also investigated. Then, this proposed method was well validated with good linearity (≥0.999), recovery (90.0-93.5%) and repeatability, and was successfully used for enantioseparation of ibuprofen in spiked plasma samples, which indicated the new column's potential usage in biological analysis.