Open Promoter Research Articles

The role of e-commerce demonstration zones in carbon emission reduction: Harmonizing economy and environment

The convergence of economic growth and environmental preservation has become a crucial objective for governments worldwide. As the digital economy helps transform industries, its carbon-reducing effect has been continuously validated. Technological innovation, policy support, and the promotion of trade openness are ways to encourage the greening of the digital economy. Currently, China is vigorously building a national e-commerce demonstration zone (EDZ). Hence, the main purpose of this study is to explore how e-commerce, as an important part of the digital economy, can help reduce carbon emissions (CE). Based on the panel data of 276 prefecture-level cities in China from 2007 to 2019, the empirical results obtained under the difference-in-difference model are that the establishment of EDZs can bring 0.0463 units of reduction in CE. The effect of suppressing CE is more significant for some regions located in the west, with larger city sizes and relatively scarce resource endowments. Distinguishing from the common paths of industrial structure, technological innovation, and optimal allocation of resources, this study also reveals the regulating role of economic openness in the mechanism analysis and finds that increasing foreign direct investment can effectively promote EDZs to reduce CE. The findings of this paper emphasize the practical significance of EDZs for energy saving and emission reduction, promoting green and high-quality development, and provide valuable references for managers and policy makers.

Sources of stress and coping strategies among Chinese medical graduate students: a qualitative study

BackgroundThe incidence of mental health problems among medical graduate students is much higher than among students of other disciplines. This can have adverse consequences for the medical students themselves as well as their future patients. This study aims to understand the pressures faced by Chinese medical students and the current status of mental health education. It also propose recommendations for the current situation and prospects for the future.MethodThe authors conducted in-depth semi-structured interviews with 22 master’s students from five medical schools during November 2023. All interview sessions were recorded and transcribed verbatim. The transcriptions were analyzed using the Colaizzi’s seven-step method.ResultThree main themes were extracted from the students’ statements: sources of psychological stress, ways to cope with stress, and perspectives on mental health education. The study showed that current mental health education in China is mostly in the form of printed mental health education manuals and mental health lectures, and there is no active tiered intervention for students at different levels. It is suggested that reforms should be made to shift to a model where the school proactively identifies problems and intervenes based on feedback.ConclusionThis study reveals the widespread psychological stress and shortcomings in current education methods. To address these challenges, institutions should develop tailored interventions, including tiered support systems, open dialogue promotion, and resilience training. Future research should focus on evaluating innovative interventions’ effectiveness, ultimately fostering a supportive environment that enhances students’ success and contributes to a healthier healthcare workforce.

Link Between Political and Economic Stability: A Case Study of Pakistan

Present research aims at investigating the relationship between political and economic stability in Pakistan. Using secondary data over the period of 1987 to 2022, ARDL bound testing technique is employed to obtain short-run and long-run estimates of the regression model. It is found that political stability and economic stability have a positive and significant relationship. The study also establishes that increase in labor force, trade openness and real discount rate help to achieve economic stability while government expenditure negatively impact economic stability. Based on these conclusions, study proposes important policy recommendations including prudent fiscal management, labor market policies for workforce growth, efforts to promote political stability, cautious monetary policy management, and continued promotion of trade openness to foster stability in economic growth.

The Impact of Digital Economy on Domestic Trade and Investment Efficiency Measurement and Heterogeneity Analysis

Against the backdrop of a new round of booming technological revolution, the digital economy has brought about high-quality development for trade. This paper is based on the 2011-2019 China's trade and investment in China, which will have a profound impact on China's trade and investment. This paper is based on the2011-2019 China. The spatial panel data of 30 provinces (excluding Tibet, Hong Kong, Macao and Taiwan) were analysed by using OLS model, panel solid model and panel data. Construct correlation regression models using fixed effects model, panel random effects model, differential GMM and systematic GMM methods. type and "Digital Economy Development Level Indicator System" to study the impact of the digital economy on the efficiency of domestic trade and investment.so as to contribute to the promotion of high-level opening up to the outside world, the deepening of domestic demand strategy, and the construction of " trade power".

THE POLITICAL INTERVENTION ON GOVERNMENT OFFICIALS’ PROMOTION IN INDONESIA

The promotion government officials comes into new era, the new policy reversed from a very closed selection process and heavily seniority-based to open, clear criteria, transparent procedures on the competency-based series test. In fact, policy implementation is not fully in favour of an administration driven. The study has two main research problems “How open promotion for local government officers conducted?” and “How does political intervention work in the open promotion mechanism?”. This paper provides comprehensive mechanism-the hybrid administration-politics model on open promotion of local bureaucrats in Indonesia. The study used a qualitative approach and assited by Nvivo 12 to reveal the most frequent concept on open promotion practices based on interviews. The data obtained through interviews with informants which selected purposively, selection committee members, candidates, and local government officers. This paper affirms interesting features. The competency series tested by independent assessors reflects an administration mechanism through merit concept. However, the absolute political final decision-making process at the governor or Mayor’s hands reflects the individual-political interest. This system shows that the administration’s process at the local level links tightly to political deliberation. The key message is that open promotion in government agencies is neither a pure merit issue nor a spoils system for competence-heavy based, on the one hand, yet is always amenable to some political intervention. The merit principles are universal; however, the practices are contextual-specific. This study has an important value in describing how The politics play significant role on local administration system.

Investigating the nature of open science practices across complementary, alternative, and integrative medicine journals: An audit.

Open science practices are implemented across many scientific fields to improve transparency and reproducibility in research. Complementary, alternative, and integrative medicine (CAIM) is a growing field that may benefit from adoption of open science practices. The efficacy and safety of CAIM practices, a popular concern with the field, can be validated or refuted through transparent and reliable research. Investigating open science practices across CAIM journals by using the Transparency and Openness Promotion (TOP) guidelines can potentially promote open science practices across CAIM journals. The purpose of this study is to conduct an audit that compares and ranks open science practices adopted by CAIM journals against TOP guidelines laid out by the Center for Open Science (COS). CAIM-specific journals with titles containing the words "complementary", "alternative" and/or "integrative" were included in this audit. Each of the eight TOP criteria were used to extract open science practices from each of the CAIM journals. Data was summarized by the TOP guideline and ranked using the TOP Factor to identify commonalities and differences in practices across the included journals. A total of 19 CAIM journals were included in this audit. Across all journals, the mean TOP Factor was 2.95 with a median score of 2. The findings of this study reveal high variability among the open science practices required by journals in this field. Four journals (21%) had a final TOP score of 0, while the total scores of the remaining 15 (79%) ranged from 1 to 8. While several studies have audited open science practices across discipline-specific journals, none have focused on CAIM journals. The results of this study indicate that CAIM journals provide minimal guidelines to encourage or require authors to adhere to open science practices and there is an opportunity to improve the use of open science practices in the field.

Dynamics of Open States and Promoter Functioning in the appY_red and appY_green Genetic Constructions Based on the pPF1 Plasmid.

Although the role of dynamic factors in DNA function still remains unclear, research in this direction is a rapidly developing area of molecular biology. In this work, the genetic constructions appY_red and appY_green, based on the plasmid pPF1 and containing a fragment of Escherichia coli (E. coli) DNA with predicted promoter-like regions, are considered complex dynamic systems in which local sites of double helix unwinding, called open states, can arise and propagate. The purpose of the article is to show the existence of a connection between the dynamics of open states and the functioning of predicted promoters. We experimentally verified the functionality of the predicted promoters using a reporter vector. Using a reverse transcription reaction, transcription start sites were identified indicating the presence of two divergent promoters, one on each strand. In mathematical studies, a dynamic model was used that described open states as one-soliton solutions (kinks) of a system of nonlinear partial differential equations, and the influence of the torque Mτ on the dynamics of kinks was taken into account. Fluorescence analysis of colonies of E. coli cells transformed with plasmid constructions showed that one of the two promoters is stronger than the other and that the strength of the promoters depends on the orientation of the fragment under study in the plasmid. On the other hand, using mathematical modeling, the energy profiles of genetic constructions were calculated and the kink trajectories were constructed. In addition, by studying the effect of torsion moment in model studies, we found threshold torque values at which the behavior of kinks changes dramatically: from oscillatory to translational motion. The minimum values of torsion moment required to initiate the transcription process were also assessed. A comparative analysis of the results of experimental data and model calculations showed a good correlation between the preferred starting points and the direction of transcription, which in turn confirmed the existence of a relationship between the dynamics of open states and the functioning of promoters.

Perturbation of the insomnia WDR90 genome-wide association studies locus pinpoints rs3752495 as a causal variant influencing distal expression of neighboring gene, PIG-Q.

Although genome-wide association studies (GWAS) have identified loci for sleep-related traits, they do not directly uncover the underlying causal variants and corresponding effector genes. The majority of such variants reside in non-coding regions and are therefore presumed to impact cis-regulatory elements. Our previously reported 'variant-to-gene mapping' effort in human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs), combined with validation in both Drosophila and zebrafish, implicated phosphatidyl inositol glycan (PIG)-Q as a functionally relevant gene at the insomnia "WDR90" GWAS locus. However, importantly that effort did not characterize the corresponding underlying causal variant. Specifically, our previous 3D genomic datasets nominated a shortlist of three neighboring single nucleotide polymorphisms (SNPs) in strong linkage disequilibrium within an intronic enhancer region of WDR90 that contacted the open PIG-Q promoter. We sought to investigate the influence of these SNPs collectively and then individually on PIG-Q modulation to pinpoint the causal "regulatory" variant. Starting with gross level perturbation, deletion of the entire region in NPCs via CRISPR-Cas9 editing and subsequent RNA sequencing revealed expression changes in specific PIG-Q transcripts. Results from individual luciferase reporter assays for each SNP in iPSCs revealed that the region with the rs3752495 risk allele (RA) induced a ~2.5-fold increase in luciferase expression. Importantly, rs3752495 also exhibited an allele-specific effect, with the RA increasing the luciferase expression by ~2-fold versus the non-RA. In conclusion, our variant-to-function approach and in vitro validation implicate rs3752495 as a causal insomnia variant embedded within WDR90 while modulating the expression of the distally located PIG-Q.

Urbanization, trade openness, and industrialization as a determent of clean energy consumption: Evidence from BRI nations

Energy and economic policies should include the intricate interconnections among urbanization, trade openness, industry, and the utilization of renewable energy. This study investigates the intricate connections among nations involved in the Belt and Road Initiative (BRI). The study employed panel econometric techniques, including DSUR, Cup-BC, and CUP-FM, to document independent variables' elasticities. Study findings suggest that urbanization is initially negatively correlated with renewable energy consumption (REC) because of the rising demand for energy, particularly non-renewable sources. Urbanization beyond a certain threshold fosters renewable energy, showcasing the positive effects of Renewable Energy Certificates (RECs). The influence of urbanization on energy use has changed throughout time. Furthermore, there is an initial positive relationship between trade openness and REC, indicating that adopting liberalized trade policies encourages the use of clean energy. Nevertheless, over time, this connection becomes less strong, suggesting a critical moment when the promotion of trade openness may lead to an increase in the use of non-renewable resources. Industrialization first stimulates the need for traditional energy sources but ultimately encourages using renewable energy. The U-invert connection demonstrates the interplay between industrialization and energy consumption patterns, emphasizing the need for flexible policies. Our study assists policymakers and stakeholders in effectively addressing energy sustainability concerns related to the Belt and Road Initiative (BRI).

Examining the effects of solar energy Innovations, information and communication technology and financial globalization on environmental quality in the united States via Quantile-On-Quantile KRLS analysis

The development of renewable energy infrastructure is widely recognized as pivotal in solving ecological concerns. However, despite their significance in advancing ecological sustainability, researchers have given scant attention to the interplay between solar energy innovations, information and communication technology (ICT), and their impacts on ecological quality. Addressing this research gap, this study examines the impact of solar energy innovations, trade openness, ICT, economic growth and financial globalization on ecological quality in the United States context. To achieve this objective, the study employs the novel Quantile on Quantile Kernel-Based Regularized Least Squares (QQKRLS) approach proposed by Adebayo et al. [1], analyzing data from 2000 to 2020. The findings from QQKRLS analysis unveil several key insights: Firstly, solar energy innovations, economic growth and trade openness exhibit a significant positive association with ecological quality. Secondly, financial globalization and ICT demonstrate a positive impact on ecological quality. Drawing from these results, the study underlines the necessity for the United States to prioritize the promotion of solar energy innovations, financial globalization, trade openness and ICT adoption. By accentuating these strategic imperatives, the United States policymakers can work towards nurturing a more sustainable ecological route while advancing economic development objectives.

Analysis of single nuclear chromatin accessibility reveals unique myeloid populations in human pancreatic ductal adenocarcinoma.

A better understanding of the pancreatic ductal adenocarcinoma (PDAC) immune microenvironment is critical to developing new treatments and improving outcomes. Myeloid cells are of particular importance for PDAC progression; however, the presence of heterogenous subsets with different ontogeny and impact, along with some fluidity between them, (infiltrating monocytes vs. tissue-resident macrophages; M1 vs. M2) makes characterisation of myeloid populations challenging. Recent advances in single cell sequencing technology provide tools for characterisation of immune cell infiltrates, and open chromatin provides source and function data for myeloid cells to assist in more comprehensive characterisation. Thus, we explore single nuclear assay for transposase accessible chromatin (ATAC) sequencing (snATAC-Seq), a method to analyse open gene promoters and transcription factor binding, as an important means for discerning the myeloid composition in human PDAC tumours. Frozen pancreatic tissues (benign or PDAC) were prepared for snATAC-Seq using 10× Chromium technology. Signac was used for preliminary analysis, clustering and differentially accessible chromatin region identification. The genes annotated in promoter regions were used for Gene Ontology (GO) enrichment and cell type annotation. Gene signatures were used for survival analysis with The Cancer Genome Atlas (TCGA)-pancreatic adenocarcinoma (PAAD) dataset. Myeloid cell transcription factor activities were higher in tumour than benign pancreatic samples, enabling us to further stratify tumour myeloid populations. Subcluster analysis revealed eight distinct myeloid populations. GO enrichment demonstrated unique functions for myeloid populations, including interleukin-1b signalling (recruited monocytes) and intracellular protein transport (dendritic cells). The identified gene signature for dendritic cells influenced survival (hazard ratio=.63, p=.03) in the TCGA-PAAD dataset, which was unique to PDAC. These data suggest snATAC-Seq as a method for analysis of frozen human pancreatic tissues to distinguish myeloid populations. An improved understanding of myeloid cell heterogeneity and function is important for developing new treatment targets in PDAC.

The Effects of Chinese Social Media on Consumer Decision-Making in the Pet Industry

The essence of social media lies in its promotion of participation, openness, communication, and community-based interactions, empowering individuals to both create and distribute content. The era when social media is pervasive in all aspects of people's lives cannot be ignored. Approximately 1.02 billion people in China were using the internet as of January 2022, compared to around 307 million users in the United States. As of January 2023, global social media usage reached 59%. This number is expected to grow as less developed digital infrastructure builds and the availability of inexpensive mobile devices increases. Pet consumer habits are no exception to the impact. Chinese social media was chosen for research due to East Asia's status as a mobile-first market. It holds the top position in global mobile social network penetration. This proposal aims to study the behavior of pet consumers in the Chinese social media environment and explore in depth how social media affects their pet consumption decisions. The study will survey Chinese people living in Australia through the questionnaire. In the questionnaire, the Chinese social media usage habits and consumption habits of pet users were quantitatively collected. By discussing the behavior habits of pet consumers on social media platforms and analyzing the consumption status and trends of the pet consumption market, this paper provides valuable data and strategic suggestions for enterprises, policy makers and academia in the pet consumption market in the context of China's social media platforms.

Preserving privacy in the era of openness: Commentary on open science requirements for identifiable data in psychological science journals.

Psychological science journals are increasingly adopting open science (OS) policies (e.g., Transparency and Openness Promotion) requiring researchers to make all data and materials publicly available in an effort to drive research toward greater transparency and accessibility. These policies certainly have many benefits to the scientific community and public in helping ensure the quality of published research. However, the Center for Open Science has not offered any explicit guidelines regarding when exceptions to OS policies should be made, with only vague guidelines offered such as "when ethical or legal constraints prevent it." We argue that these ambiguous policies may create bias in decisions made by journal editors as to whom and what type of research is granted exceptions. When journals are too rigid in their exception policies, this may unintentionally contradict OS's goals to create a more valid and ethical science. We argue that journals should never mandate identifiable data to be posted publicly as a publication prerequisite. Maintaining participant anonymity should always come before OS policies to (a) align with psychologists' primary obligation of maintaining participant confidentiality, (b) encourage participation from the broader population and more specifically from marginalized communities, and (c) maintain unbiased, representative, and valid data. From empirical and ethical insights, we offer several solutions to ease the tensions between OS and participant privacy during the data collection and publication process. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Scientific journals: guidelines for creating a data collection

This article aims to suggest actions for the opening of scientific data, deposit, use and dissemination, so that scientific journals, linked to Higher Education Institutions, create open data collections. It is an applied social research, with a qualitative, exploratory, documentary approach and with action-research dimensions. A survey of the scientific data openness policies of the journals that use the Dataverse repository of Harvard University was carried out, based on the requirements of the quality seal of reliable digital repositories Core Trust Seal and the Transparency and Openness Promotion Guidelines. The information obtained in this survey supported the implementation of action-research steps, through which guidelines were formulated to meet the proposed objective. With such results, other journals will be able to join the movement of open data adjacent to the articles they publish, expanding the scientific practices related to the movement for open science and the democratization of scientific knowledge.

Evaluation of Transparency and Openness Guidelines in Physical Therapist Journals.

The goals of this study were to evaluate the extent that physical therapist journals support open science research practices by adhering to the Transparency and Openness Promotion (TOP) guidelines and to assess the relationship between journal scores and their respective journal impact factor (JIF). Scimago, mapping studies, the National Library of Medicine, and journal author guidelines were searched to identify physical therapist journals for inclusion. Journals were graded on 10 standards (29 available total points) related to transparency with data, code, research materials, study design and analysis, preregistration of studies and statistical analyses, replication, and open science badges. The relationship between journal transparency and openness scores and their JIF was determined. Thirty-five journals' author guidelines were assigned transparency and openness factor scores. The median score (interquartile range) across journals was 3.00 out of 29 (3.00) points (for all journals the scores ranged from 0 to 8). The 2 standards with the highest degree of implementation were design and analysis transparency (reporting guidelines) and study preregistration. No journals reported on code transparency, materials transparency, replication, and open science badges. TOP factor scores were a significant predictor of JIF scores. There is low implementation of the TOP standards by physical therapist journals. TOP factor scores demonstrated predictive abilities for JIF scores. Policies from journals must improve to make open science practices the standard in research. Journals are in an influential position to guide practices that can improve the rigor of publication which, ultimately, enhances the evidence-based information used by physical therapists. Transparent, open, and reproducible research will move the profession forward by improving the quality of research and increasing the confidence in results for implementation in clinical care.

DDX5 (p68) orchestrates β-catenin, RelA and SP1 mediated MGMT gene expression in human colon cancer cells: Implication in TMZ chemoresistance

DDX5 (p68) upregulation has been linked with various cancers of different origins, especially Colon Adenocarcinomas. Similarly, across cancers, MGMT has been identified as the major contributor of chemoresistance against DNA alkylating agents like Temozolomide (TMZ). TMZ is an emerging potent chemotherapeutic agent across cancers under the arena of drug repurposing. Recent studies have established that patients with open MGMT promoters are prone to be innately resistant or acquire resistance against TMZ compared to its closed conformation. However, not much is known about the transcriptional regulation of MGMT gene in the context of colon cancer. This necessitates studying MGMT gene regulation which directly impacts the cellular potential to develop chemoresistance against alkylating agents. Our study aims to uncover an unidentified mechanism of DDX5-mediated MGMT gene regulation. Experimentally, we found that both mRNA and protein expression levels of MGMT were elevated in response to p68 overexpression in multiple human colon cancer cell lines and vice-versa. Since p68 cannot directly interact with the MGMT promoter, transcription factors viz., β-catenin, RelA (p65) and SP1 were also studied as reported contributors. Through co-immunoprecipitation and GST-pull-down studies, p68 was established as an interacting partner of SP1 in addition to β-catenin and NF-κB (p50-p65). Mechanistically, luciferase reporter and chromatin-immunoprecipitation assays demonstrated that p68 interacts with the MGMT promoter via TCF4-LEF, RelA and SP1 sites to enhance its transcription. To the best of our knowledge, this is the first report of p68 as a transcriptional co-activator of MGMT promoter and our study identifies p68 as a novel and master regulator of MGMT gene expression.

ChatGPT Isn't Magic

ChatGPT Isn't Magic

35 Years of TFAM Research: Old Protein, New Puzzles.

Transcription Factor A Mitochondrial (TFAM), through its contributions to mtDNA maintenance and expression, is essential for cellular bioenergetics and, therefore, for the very survival of cells. Thirty-five years of research on TFAM structure and function generated a considerable body of experimental evidence, some of which remains to be fully reconciled. Recent advancements allowed an unprecedented glimpse into the structure of TFAM complexed with promoter DNA and TFAM within the open promoter complexes. These novel insights, however, raise new questions about the function of this remarkable protein. In our review, we compile the available literature on TFAM structure and function and provide some critical analysis of the available data.

Open Science Standards at Journals that Inform Evidence-Based Policy.

Evidence-based policy uses intervention research to inform consequential decisions about resource allocation. Research findings are often published in peer-reviewed journals. Because detrimental research practices associated with closed science are common, journal articles report more false-positives and exaggerated effect sizes than would be desirable. Journal implementation of standards that promote open science-such as the transparency and openness promotion (TOP) guidelines-could reduce detrimental research practices and improve the trustworthiness of research evidence on intervention effectiveness. We evaluated TOP implementation at 339 peer-reviewed journals that have been used to identify evidence-based interventions for policymaking and programmatic decisions. Each of ten open science standards in TOP was not implemented in most journals' policies (instructions to authors), procedures (manuscript submission systems), or practices (published articles). Journals implementing at least one standard typically encouraged, but did not require, an open science practice. We discuss why and how journals could improve implementation of open science standards to safeguard evidence-based policy.

Eclectic-Procedural Innovation Management Approach with Regard to Community Management Within the German-Language Gaming Industry

The gaming industry faces several challenges, such as inefficient development processes, shorter product lifecycles, dynamic knowledge, high staff turnover, and heterogeneous IT structures. To address these issues, this paper proposes an eclectic-procedural innovation management approach that integrates knowledge management methods and processes for the optimal cybernetic systematization of innovation management elements within the German-language gaming industry. Drawing on the human, organizational, and technological (HOT-Fit) model, this approach aims to enable gaming companies to analyze, assess, and optimize innovation management using agile and adaptive methods. The concept also involves modifications to the matrix organization and emphasizes the importance of open innovation, community management, and promoter functions. The eclectic approach offers several benefits, including greater efficiency in innovation processes, reduced risks, the exploitation of knowledge and innovation potential, and the preservation of innovation power. However, a shift in mindset is required to systematically implement this approach in a process-oriented manner, focusing on improving organizational skills and adapting to the changing landscape of the gaming industry.