The purpose of this paper is to report the presence of a species of Opalina in Costa Rica, previously described from Agalycbnis moreletei of Guatemala. Three other species of Opalina are known in Costa Rica, all described from autochthonous frogs. O. helenae Metcalf, from Agalycbnis belenae Cope captured at San Carlos, O. copei Metcalf, from Rana copei Boulanger, from Pico Blanco and O. obtrigonoidea austricola Metcalf, found in Rana pipiens collected at the same place.