Online Software Research Articles

Enhancing PDF interaction for a more engaging user experience in library: Introducing ChatPDF

The paper introduces ChatPDF as a solution to the limited interaction capabilities of traditional PDF readers in library systems. It highlights the benefits of ChatPDF for enhancing user engagement and satisfaction with digital resources in libraries, and identifies potential areas for implementation and opportunities for further research. The paper addresses the issue of limited PDF interaction capabilities of traditional PDF readers in library systems and their potential to cause frustration and disengagement among users. It proposes ChatPDF, an online software platform that leverages ChatGPT API to offer a more intuitive and natural way of engaging with PDF documents. The paper also outlines the various features and advantages of ChatPDF and its potential impact on library systems. The paper concludes that ChatPDF offers a more advanced and user-friendly approach to interacting with PDF files than traditional PDF readers. It recommends libraries to consider implementing ChatPDF to enhance user engagement and satisfaction with their digital resources. The paper also identifies limitations and opportunities for future research in evaluating the impact of ChatPDF on user experience and library systems. This paper introduces ChatPDF as a solution to the limited PDF interaction capabilities in library systems, highlighting its unique features and benefits, such as summarization, recommendations, multi-lingual support, and AI assistance. It also discusses the potential impact of ChatPDF implementation on library systems, making it an original and valuable contribution to the field of library science.

#5736 IDENTIFICATION AND CHARACTERIZATION OF URINARY AND SERUM AMINOACIDS IN DIABETIC KIDNEY DISEASE PATIENTS USING MS-HPLC

Abstract Background and Aims Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease, but few biomarkers of early identification of DKD are available. The aim of the study was to asses new urinary and plasma biomarkers in early stages of DKD, especially in normoalbuminuric type 2 diabetes mellitus (DM) patients. Method This cross-sectional pilot study performed an integrated metabolomic profiling of blood and urine in 90 patients with type 2 DM, classified into 3 stages (30 normo-, 30 micro-, and 30 macroalbuminuric, respectively) and 30 age- and gender-matched healthy controls through high-performance liquid chromatography and mass spectrometry (UPLC-QTOF-ESI* MS). Metabolites were discovered and processed by the advanced Metaboanalyst 5.0. online software using multi- and univariate untargeted analysis. Both multivariate and univariate bioinformatics confirmed the relevance of certain amino acids as biomarkers to be responsible for the occurrence of early renal involvement in the course of type 2 DM. Results A total of 5 metabolites, namely Taurine, Tiglylglycine, Tryptophan, Kynurenic acid, and L-acetylcarnitine were discovered as potential biomarkers for the early identification of DKD. UPLC-QTOF-ESI* MS revealed that: (1) the urinary amino acids levels were increased in normoalbuminuric type 2 DM patients as compared with healthy control subjects; (2) a number of key biomarkers have been identified in the urine, which reflect kidney injury at specific sites along the nephron, including glomerular injury and tubular damage, oxidative stress, inflammation, and activation of the intrarenal renin-angiotensin system. Conclusion High-performance liquid chromatography coupled with mass spectrometry showed that the urinary amino acids levels were increased in normoalbuminuric type 2 DM patients. The results of the study provide a particular metabolomic profile related to blood and urine metabolites which could impact both the glomeruli and the tubules, even in the early stages of DKD.

Participatory monitoring in farmer field school program through Whatsapp among indigenous farmers in rural Sarawak, Malaysia

ABSTRACT This paper aims to assess the use of WhatsApp Group for participatory monitoring in a System of Rice Intensification – Farmer Field School (SRI-FFS) program involving the rice farming community in the rural Bidayuh village of Tebaro in Sarawak, Malaysia. Descriptive analysis was performed on a 182-day WhatsApp Group chat taking place from 17 September 2019 to 16 March 2020. The data were analyzed using two online softwares to generate data visualizations. The study revealed that the strengths of participatory monitoring using WhatsApp Group were found in the principles of flexibility and methodologically eclectic. For flexibility, four factors indicated were technical sharing, personal sharing, comments on current events, and the addition of new members. The methodologically eclectic elements were identified as messages in the forms of texts, media, emoji and links. Limitations for participatory monitoring were in the elements of participation and negotiation due to the role-based group structure. To effectively promote the use of digital platforms, agricultural policies must promote inclusiveness and prioritize equitable access to ICT devices and facilities. Current findings show that social media can enhance active multi-stakeholder participation but should be complemented with non-ICT means of communication for rural farming communities.

New 3-Aminopropylsilatrane Derivatives: Synthesis, Structure, Properties, and Biological Activity.

The biologically active compound 3-aminopropylsilatrane (a compound with a pentacoordinated silicon atom) underwent an aza-Michael reaction with various acrylates and other Michael acceptors. Depending on the molar ratio, the reaction yielded Michael mono- or diadducts (11 examples) containing functional groups (silatranyl, carbonyl, nitrile, amino, etc.). These compounds were characterized via IR and NMR spectroscopy, mass spectrometry, X-ray diffraction, and elemental analysis. Calculations (using in silico, PASS, and SwissADMET online software) revealed that the functionalized (hybrid) silatranes were bioavailable, druglike compounds that exhibited pronounced antineoplastic and macrophage-colony-stimulating activity. The in vitro effect of silatranes on the growth of pathogenic bacteria (Listeria, Staphylococcus, and Yersinia) was studied. It was found that the synthesized compounds exerted inhibitory and stimulating effects in high and low concentrations, respectively.

Impact of Online Chat Language on Second Language Learners' written composition: A Corpus Based-Study

This study investigates the impact of online chat language on second language learners and has gained significant attention in the field of language acquisition. The study examines how online chat language influences learners' proficiency, accuracy, vocabulary acquisition, and sociolinguistic competence. By analyzing relevant research studies conducted using the corpus-based methodology, the study aims to provide insights into the advantages and challenges associated with online chat language for second language acquisition. A sample of the corpus of online chat conversations via WhatsApp chat will be compiled, representing various language learners' interactions and finding out the KWIC through online software programmer Sketch Engine. The corpus analysis will be complemented questionnaire conducted with a subset of participants. The findings of this study will contribute to our understanding of the role of online chat language in second language acquisition and provide insights for language learners.

Mental Imagery Between Indigenous & Ball Games Players: A Comparison

Background: - Ball game is a very popular game all over world and indigenous sport also very popular in India. Indigenous sports are native games and play activities originating in and Characteristic of an area or country. Aims: - The Purpose of the study is to compare indigenous & ball games players in respect of sports imagery ability. Methods and Material: - Total 40 Inter University level players from Visva- Bharati University, Santiniketa, Birbhum, WestBengal, India were selected as subjects for this study. The age of the subjects were 21-25 years. Statistical analysis used :- Descriptive statistics and‘t’ test were used. To calculate the collected data socscistatistics.com (Online software) was used. Results:- The result shows that there is no significant difference of sports mental imagery ability between indigenous sports(kho-kho&kabaddi) and ball game(football&volleyball) players. Conclusions:- There is no significant difference between indigenous sports and ballgame players in respect of Sports Mental imagery ability.

Fast Pace Sun Salutations (FPSS) Yoga Postures Influence Brain waves Activity in Addition to Heart Rate Variability Pattern: A Pilot Study

ABSTRACT Background: Sun salutation (Surya namaskar) is an ancient yogic exercise, which is a combination of 12 different postures rhythmically. Yoga practitioners perform this procedure in a slow as well as fast pace manner. Aim: We undertook this study to find out the immediate changes in heart rate variability (HRV) and brain waves spectrum after the practice of fast pace sun salutation (FPSS). Settings and Design: This prospective interventional observational study was conducted on 30 male healthy subjects in the 30–45 years age group from September 2021 to December 2021 after approval from the Institutional Ethics Committee. Participants gave their written consent in the prescribed format. Materials and Methods: Fourteen rounds of FPSS were performed in approximately 10 min under the guidance of a yoga expert. Brain Tap Dinamika Machine (Advanced HRV Test System, Moscow, Russia) was used to assess different HRV and brain waves parameters before and after FPSS. Statistical Analysis Used: Statistical analysis was done by Student’s paired t-test using GraphPad online software. Results: All independent variables of time and frequency domain analysis of HRV were found to be increased after the intervention. A significant upsurge in low frequency (LF)% as compared to high frequency (HF)% and very low frequency (VLF)% showed a relative increase in sympathetic activity. In brain waves, delta, theta, and alpha were decreased, whereas activities of beta and gamma waves were found to be increased. Conclusions: Increase in both LF and HF components showed that both sympathetic and parasympathetic components have increased. Brain wave analysis suggested increased mental alertness after FPSS.

A SYSTEMATIC REVIEW OF REHABILITATION TECHNIQUES FOR ADULT PATIENTS UNDERGOING EXTERNAL FIXATOR TREATMENT FOR LIMB RECONSTRUCTION OF THE LOWER LIMB

IntroductionExternal fixators are common surgical orthopaedic treatments for the management of complex fractures and in particular, the use of circular frame fixation within patients requiring limb reconstruction. It is well known that common complications relating to muscle length and patient function without rehabilitation can occur. Despite this there remains a lack of high-quality clinical trials in this area investigating the role of physiotherapy or rehabilitation in the management of these patients. We aim to complete a systematic review of rehabilitation techniques for patients undergoing external fixator treatment for Limb Reconstruction of the lower limb.Materials & MethodsA comprehensive search of AMED, CINAHL, MEDLINE and COCHRANE databases was conducted to identify relevant articles for inclusion, using a search strategy developed in collaboration with a research librarian. Inclusion criteria consisted of adults aged 18 years and over who have experienced leg trauma (open fracture, soft tissue damage), elective leg deformity corrective surgery, bone infection or fracture non-union who have been treated with the use of an external fixator for fixation. Specific exclusion criteria were patients below the age of 18 years old, patients with cancer, treatment of the injury with internal nail, patients who underwent amputation, the use of external fixators for soft tissue contracture management, editorials, comment papers, review papers, conference proceedings and non-English papers.Titles, abstracts, and full texts were screened for suitability by pairs of reviewers according to the inclusion and exclusion criteria using Rayyan QCRI online software. Any conflicts were resolved through discussion with three independent specialist senior reviewers. Following full text screening, references lists of included articles were manually searched to ensure that all relevant studies were identified. Due to lack of evidence, forward searching was also completed for studies included in the review. Data quality was assessed using the mixed methods appraisal tool and the CERT assessment tool was utilised to look at completeness of reporting of exercise interventions.ResultsA total number of 832 articles were initially retrieved from our search once duplicate articles removed. After title and abstract screening, 45 articles remained for full text screening. Of these, 11 articles met our inclusion criteria and included for data extraction.ConclusionsWe expect high variability of results due to our inclusion criteria and therefore plan to conduct a narrative synthesis to summarise the findings whilst measing against the mixed methods appraisal tool and CERT assessment scores to assess the data quality. We anticipate lower assessment scores within the fewer articles found and therefore poorer-quality data. We currently are in the process of finalising this data extraction. This will be completed ready for submission and potential presentation at the BLRS conference in March 2023.

Genetic Variants of CYP4F2 Associated with Ischemic Stroke Susceptibility in the Han Population from Southern China.

The pathophysiological mechanism of ischemic stroke is complex. Traditional risk factors cannot fully or only partially explain the occurrence and development of IS. Genetic factors are getting more and more attention. Our study aimed to explore the association between CYP4F2 gene polymorphism and susceptibility to IS. A total of 1322 volunteers were enrolled to perform an association analysis through SNPStats online software. Using FPRP (false-positive report probability) to detect whether the result is a noteworthy finding. The interaction of SNP-SNP in IS risk was assessed by multi-factor dimensionality reduction. Statistical analysis of this study was mainly completed by SPSS 22.0 software. Mutant allele "A" (OR = 1.24) and genotype "AA" (OR = 1.49) or "GA" (OR = 1.26) of CYP4F2-rs2108622 are risk genetic factors for IS. Rs2108622 is significantly associated with an increased risk of IS among subjects who are females, aging >60 years old, with BMI ≥24 kg/m2, and smoking or drinking volunteers. CYP4F2-rs3093106 and -rs3093105 are associated with susceptibility to IS among smoking, drinking subjects, or IS patients complicated with hypertension. CYP4F2-rs2108622, -rs3093106, and -rs3093105 are associated with an increased risk of IS.

Astrocyte-derived complement C3 is activated in patients with tuberous sclerosis complex and mediates immune injury: an integrated bioinformatics analysis.

Tuberous sclerosis complex (TSC) is a genetic disorder associated with multiple neurological manifestations. Cortical tubers (CT) are recognized as the hallmark brain lesions of TSC and contribute to neurological and psychiatric symptoms. To understand the molecular mechanism of neuropsychiatric features of TSC, the differentially expressed genes (DEGs) in CT from patients with TSC and those in normal cortex (NC) from participants acting as healthy controls were investigated. The dataset of GSE16969, which had already been published and described (https://onlinelibrary.wiley.com/doi/10.1111/j.1750-3639.2009.00341.x), was downloaded from the Gene Expression Omnibus (GEO), including samples of 4 CT and 4 NC. The R package "limma" was used to screen DEGs in CT and NC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analyses of the DEGs were conducted using the R package "clusterProfiler". The online software Ingenuity Pathway Analysis (IPA) was used to explore activation/inaction of canonical pathways. The hub gene was selected based on the protein-protein interaction (PPI) network constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database and Cytoscape software. Subsequently, the hub genes at messenger (mRNA) and transcriptional levels were tested. We also explored immune cell-type enrichment using the online database xCell, and assessed the correlation between cell types and C3 expression. Then, we verified the source of C3 by constructing TSC2 knockout cells in the U87 astrocyte cell line. The human neuronal cell line SH-SY5Y was used to examine the effects of excessive complement C3 levels. A total of 455 DEGs were identified. A large number of pathways were involved in the immune response process based on the results of GO, KEGG, and IPA. C3 was identified as a hub gene. Complement C3 was also upregulated in the human CT and peripheral blood. Furthermore, based on the enrichment of functions and signaling pathways, complement C3 played a critical role in immune injury in CT of TSC. In the in vitro experiments, we found that excessive complement C3 was derived from TSC2 knockout U87 cells, and there was an increased intracellular reactive oxygen species (ROS) level in SH-SY5Y cells. Complement C3 is activated in patients with TSC and can mediate immune injury.

Divulging the Lived Experiences of Public School Teachers in the U.S.A. during COVID-19 Pandemic: Phenomenological Analysis

This research examined the lived experiences of public school teachers in the United States during the COVID-19 pandemic. A qualitative design was performed using interpretative phenomenological analysis. Twenty public school teachers in the United States formed the sample, with the inclusion criterion being a minimum of three years’ teaching experience, including the pandemic. To meet the safety measure protocols set by the U.S. government, the data gathering was conducted online using Microsoft Forms. The semi-structured interviews comprised two sections: the first ascertained the respondents’ profiling and the second revealed their first-hand experiences. The thematic analysis approach was utilized to analyze the data. The findings disclosed five emerging themes: 1) low student engagement; 2) unstable internet connection; 3) professional development as school district support; 4) utilization of online engagement tools to leverage classroom engagement; and 5) evident parental support. School districts, teachers, parents, students, and potential researchers were provided with recommendations for improving online and hybrid instruction. In addition to implementing socio-emotional learning programs and addressing slow internet connections, it is advised that school districts train teachers on how to use the tools, software, and techniques for online and hybrid instruction. Teachers who are having difficulty in adjusting to online learning should think about developing their capabilities for helping students while finding a healthy balance in terms of stress management. The use of technology to support students should be adequately taught to parents. Future studies on the pandemic's effects on education should continue.

POINT CLOUD CLASSIFICATION OF AN URBAN ENVIRONMENT USING A SEMI-AUTOMATIC APPROACH

Abstract. In this contribution a point cloud classification in an urban context has been presented. The aim of the work is to test a semi-automatic classification approach and to verify its usefulness in the scan-to BIM process, and to validate how much it is straightforward for the definition of different point cloud LODs. The work methodology is structured in three phases. The first concerns data acquisition and processing through geomatic instruments and methodologies that guarantee a complete and expeditious survey such as ground-based MMS and UAV for aerial photogrammetry. The second phase concerns the testing of an online software that per-forms point cloud classifications through AI algorithms. The system allows either to use standard classifiers that are already available, or to create a customizable catalogue of the different classes that one wants to attribute to the urban scene. Following the automatic classification process, where all objects have been identified, manual corrections can be made to improve the classification of objects into specific classes. The third step is object detection and extraction. Here, the relationship between automatic classification, point cloud density, object identification and the various degrees of LOD definition was explored. The higher the LOD, the greater the number of objects that can be identified, particularly those elements related to street furniture and urban facilities. Once these objects have been classified, it is then possible to extract them in interoperable format. This allows such data to be managed and shared through BIM platform.

Do Pregnant Women Perceive Being Counseled About Nutrition by Healthcare Providers? A Cross-Sectional Study of the Recall and Satisfaction of Nutrition Counseling of Patients Throughout the Course of Their Pregnancy

The Academy of Nutrition and Dietetics identifies a balanced diet and appropriate weight gain as two important components of a healthy pregnancy. The aim of this study was to evaluate the recall of pregnant women about nutrition counseling provided by their healthcare provider during their pregnancy. We conducted an anonymous, voluntary survey using online software for women who had recently given birth and were less than 2 weeks postpartum. Discrete variables were analyzed using Chi-square or Fisher’s exact test. Analyses were carried out using R statistical software. Differences were considered statistically significant at P < .05. Of 201 postpartum women surveyed, 100 (49.8%) recall receiving nutrition counseling during pregnancy, while 101 (50.2%) did not recall receiving nutrition counseling. Notably, 73% of women who were counseled about nutrition during pregnancy changed their behavior based on provider advice. These findings suggest that approximately half of women polled recall receiving nutrition counseling during the pregnancy, and that there are crucial opportunities for providers to influence positive behavior change in their patients. Additional research is needed to determine perceptions of providers’ counseling and potential needs in providers’ knowledge, skills, and attitudes toward nutrition counseling, particularly across different demographic groups and social determinants of health.

(429) A Pilot Assessment of Urologic Surgery Resident Attitudes on Utilization of MyTipReport Feedback Software in Inflatable Penile Prosthesis Placement Surgery- A single-site pilot study

Abstract Introduction MyTipReport is an innovative online software that allows for capturing, sharing, and reviewing feedback in medical education amongst students, residents, and educators. There has been extensive research into the utilization and integration of this software into medical residency programs to evaluate and provide feedback to learners. However, there is a paucity of literature regarding evaluation of resident attitudes and beliefs in the role this software plays to establish surgical competency. Objective At a single, high-volume institution performing Inflatable Penile Prosthesis cases, our objective is to evaluate resident attitudes regarding utilization of MyTipReport in their competency assisting and performing Inflatable Penile Prosthesis operations. Methods From December 2021 to May 2022, residents were evaluated using MyTipReport during all resident staffed penile prosthesis surgeries. Residents were evaluated through a number of crucial steps during Inflatable Penile Prosthesis placement. Trainees who have utilized MyTipReport then completed a standardized survey to assess if the feedback system provided by MyTipReport has provided benefit in their training, specifically in regard to Inflatable Penile Prosthesis placement surgery, Figure 1. Two-tailed t-test was used to determine difference in mean survey response score between sub-groups. P value <0.05 was used to define significance. Results A total of 7 residents performed 44 penile implant surgeries at a single Urology residency program and utilized MyTipReport for feedback. All 7 completed the survey. Of the included cohort, 28.6% of respondents (n=2) were senior residents, and 71.4% were junior residents (n=5). Additionally, 57.1% (n=4) respondents were male and 42.9% (n=3) were female. Mean survey responses for the cohort are summarized in Figure 2. All 7 respondents answered Question 9 and Question 10 with “5 - Overall Positive”, with a mean response of 5. For Questions 1-8 of the survey, no statistically significant difference in mean response was noted between senior residents and junior residents (p>0.05), as depicted in Table 1. When stratified between male and female residents, there was a statistically significant difference in mean response for Question 2, “How do you feel MyTipReport feedback has impacted your confidence as a resident on Inflatable Penile Prosthesis placement surgeries?”, with a mean response of 3.5 for the male residents versus 5 for the female residents, respectively (p<0.05), shown in Table 1. Conclusions Initial incorporation of the MyTipReport feedback software in a single-site Urology Residency program as a means of evaluating and providing feedback in IPP placement operations shows promising early results. Resident attitudes toward using the MyTipReport software to receive feedback are overall positive. Understanding ways to improve and optimize the utilization of MyTipReport in Urology residency program education should be the focus of future studies prior to expanded application and implementation. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Run Wang, MD, FACS is a consultant for Boston Scientific and Teleflex.

Food consumption based on processing level (according to Nova system) during the COVID-19 pandemic among adolescents with immunocompromised conditions: a case-control study.

The COVID-19 pandemic impacts on eating habits among adolescents may be more relevant in pediatric patients with immunocompromised chronic diseases. This case-control study conducted between June and October 2020 aimed to: (i) describe dietary patterns of adolescents with chronic conditions compared to healthy controls and (ii) determine associations between food consumption, health-related quality of life (HRQL) and sleep quality during the COVID-19 pandemic. Participants (184 immunocompromised and 58 healthy adolescents, aged 14.3 [SD 2.5]) responded to HRQL and sleep validated instruments (PedsQL and PSQI) and three 24 h food recalls via online software. Adjusted linear and logistic regressions were used to assess differences in dietary patterns and associations between food consumption (according to Nova classification) and HRQL and sleep quality. Adolescents with gastrohepatic, rheumatic, and kidney diseases had an improved dietary pattern vs. their healthy peers, showing greater consumption of unprocessed and minimally processed foods (unstandardized coefficient (b) = 7.35%[95%CI 1.59; 13.1]; b = 15.10%[95%CI 7.00; 23.1]; and b = 11.2%[95%CI 5.68; 16.8]), and lower consumption of ultraprocessed foods (b = -7.53%[95%CI-12.90; -2.18]; b = -11.4%[95%CI-18.90; -3.94]; b = -10.8%[95%CI-16.00; -5.68]). Consumption of culinary ingredients was associated with reduced psychological HRQL in controls (standardized coefficient (β) = -0.26[95%CI-0.52; -0.004]), and processed food consumption was associated with improved sleep latency in immunocompromised participants (β = 0.16[95%CI 0.01; 0.31]). These findings suggest diet quality may play a role in HRQL and sleep quality in this population, and may be relevant for clinical practitioners and policy makers when considering the importance of dietary quality in immunocompromised youths.

O111 Defining humanitarian surgery: an international Delphi process

Abstract Introduction Over the last decade there has been an exponential increase in the terms “global surgery” and “humanitarian surgery”. However, the terms are often used interchangeably and there is no fixed definition of ‘humanitarian surgery’. This makes it difficult to compare interventions, outcomes and cost effectiveness since a wide variety of activities are included in these global or humanitarian surgery programmes. Methods A Delphi process was used over 3 rounds using online software to facilitate responses. A core set of academics and global surgery leaders were invited by targeted email and a snowball sampling effect was used to target responses form Low/Middle Income Countries. Predetermined inclusion criteria were used to ensure the validity of respondents. A Likert scale of 1-6 was used, group agreement was indicated by a median of 3 4 and group consensus was indicated by an interquartile range of £ 2. Intraclass correlation was used to assess stability of response. Results Round 1 generated 25 statements, across 4 themes: Who, What, Where and When. Of these, 10 reached agreement and consensus after Rounds 2 and 3. These 10 statements were then used as the basis for the definition of ‘Humanitarian Surgery’. Conclusion By agreeing the definition of ‘Humanitarian Surgery’, this will allow comparison of activities within the field to ensure they are appropriate, provide a high quality of care and are cost effective. Debate should now move to focus on ensuring Humanitarian Surgery is delivered to the highest possible standard wherever and whenever it is required.

Genetic analysis of a Chinese pedigree affected with Congenital coagulation factor XII deficiency due to a c.1A>G start codon variant of F12 gene

To explore the clinical characteristics and genetic etiology of a consanguineous Chinese pedigree affected with Congenital coagulation factor XII (XII) deficiency. Members of the pedigree who had visited Ruian People's Hospital on July 12, 2021 were selected as the study subjects. Clinical data of the pedigree were reviewed. Peripheral venous blood samples were taken from the subjects. Blood coagulation index and genetic testing were carried out. Candidate variant was verified by Sanger sequencing and bioinformatic analysis. This pedigree has comprised 6 individuals from 3 generations, including the proband, his father, mother, wife, sister and son. The proband was a 51-year-old male with kidney stones. Blood coagulation test showed that his activated partial thromboplastin time (APTT) was significantly prolonged, whilst the FXII activity (FXII:C) and FXII antigen (FXII:Ag) were extremely reduced. The FXII:C and FXII:Ag of proband's father, mother, sister and son have all reduced to about half of the lower limit of reference range. Genetic testing revealed that the proband has harbored homozygous missense variant of c.1A>G (p.Arg2Tyr) of the start codon in exon 1 of the F12 gene. Sanger sequencing confirmed that his father, mother, sister and son were all heterozygous for the variant, whilst his wife was of the wild type. By bioinformatic analysis, the variant has not been included in the HGMD database. Prediction with SIFT online software suggested the variant is harmful. Simulation with Swiss-Pbd Viewer v4.0.1 software suggested that the variant has a great impact on the structure of FXII protein. Based on the Standards and Guidelines for the Interpretation of Sequence Variants: A Joint Consensus Recommendation of the American College of Medical Genetics and Genomics (ACMG), the variant was rated as likely pathogenic. The c.1A>G (p.Arg2Tyr) variant of the F12 gene probably underlay the Congenital FXII deficiency in this pedigree. Above finding has further expanded the spectrum of F12 gene variants and provided a reference for clinical diagnosis and genetic counseling for this pedigree.

Cytotoxic behavior and DNA/BSA binding activity of thiosemicarbazone based Ni(II) complex: bio-physical, molecular docking and DFT study

The bidentate ligand thiosemicarbazone (H3L) and its Ni(II) complex were synthesized and characterized by single crystal X-ray diffraction and various spectroscopic methods. The Ni(II) complex exhibits almost square planar geometry around the nickel ion, coordinated by two ligands through N & S donor centers. The important structural features of single crystal of the complex are [space group: P-1; a = 11.0594(9) Ǻ; b = 12.2339(9) Ǻ; c = 14.8835(9) Ǻ; α = 110.857(6); β = 91.029(6) and γ = 108.710(7)]. The DFT/TD-DFT simulations have been performed to determine the HOMO-LUMO energy, electronic spectroscopy data, the minimum energy optimized structure and to calculate the reactivity descriptors of the compounds using the B3LYP level of theory. The DFT and NBO analysis based on theoretical investigation helps to locate the MEP surface and bonding mode of the ligand in complex respectively. Different reactivity descriptors establish a correlate between their reactivity and bio-molecular affinity of the molecules. The binding abilities of the compounds with DNA and BSA were within the order of standard DNA and BSA binders. The bio-molecular conformational change as well as interactions of the compounds were monitored by biophysical analyses like; 3D fluorescence, FRET calculations and anisotropy studies with BSA. The potent drug likeness antineoplastic and bio-activity of the H3L and Ni(II) complex were assessed using online software PASS and ADMET. The antiproliferative activity of the compounds was evaluated against the normal human embryonic kidney (HEK293) cells and the human breast carcinoma (MCF-7) cancer cell line. The Annexin V-FITC, Propidium iodide assay in the presence of the free ligand H3L and its Ni(II) complex were performed using flow cytometric method for their cell death mechanisms. Reactive Oxygen Species (ROS) production assay using 2′,7′-Dichlorodihydrofluorescein diacetate (DCFDA) was evaluated in presence of the compounds.

The Methodology of Delayed Interaction Techniques Application in L2 Asynchronous Learning and Teaching

In the new global education, online learning and teaching have become central issues for different methodology development. This paper examines the ways for asynchronous foreign language teaching mode. 
 The purpose of this study is to define specific techniques that are applicable to asynchronous interaction in the process of foreign language learning and teaching. The aim of this paper is also to work out the methodology for their use. 
 Methodology. For this study, a number of research methods were used. The previous studies' analysis was used for the examination of the recent findings. A qualitative case-study experiment was used to investigate personal teachers and students’ experience with delayed interaction techniques (DIT) use in the process of Foreign Language (FL) Methodology teaching and learning that is a component of FL teachers training course in the department of foreign languages of Pavlo Tychyna Uman State Pedagogical University in Ukraine. The methodological approach offered an effective way of compiling delayed interaction technique stages. Data were gathered from the students’ feedback survey that was used as a qualitative method. The online software of the Voyant tool, one of the more practical ways for survey results visualization was applied. 
 Results. One of the main findings of the current study is a clarification of the delayed interaction technique notion, which is suggested as a definition in the context of the current study issue. The second finding is developing the methodology of using DIT in a second language (L2) asynchronous learning and teaching mode of studying. The methodology includes seven steps for DIT application.
 Conclusions. This study has found that generally, interaction is possible to use even asynchronically in conditions that prevent immediate interaction. The results of this study have no limitations for application according to educational institutions, and learners’ age of L2 level. However, for effective DIT applications, it is necessary for teachers and students to have enough digital skills, internet and gadgets access. Further work needs to be done to examine more online resources such as software, apps, and tools for using them in asynchronous L2 learning and teaching.

Opportunities in the Pet Smart Device Market

In order to conduct this research, we identified the popularity of the pet supply market, and developed a product that could be used to locate pets through collars, check their location in real time through online software, and serve as a social platform for users. After several weeks of market research, through questionnaires and comparison, and to broaden the usefulness of the product, we concluded that pet owners need professional services to help them keep their pets healthy. Therefore, a health monitoring function was added to the product. After that, we added more sub-products based on the features that we learned through the questionnaire that our target customers needed and were interested in, and to confirm the cost and feasibility of the product.