Ongoing Cognitive Processes Research Articles

The effects of experimental pain on episodic memory and its top-down modulation: a preregistered pooled analysis.

Pain can automatically interfere with ongoing cognitive processes such as attention and memory. The extent of pain's negative effects on cognitive functioning seems to depend on a balance between top-down and bottom-up factors. In this large, preregistered, pooled reanalysis of 8 studies, we investigated the robustness of the detrimental effect of acute pain on recognition memory and whether top-down mechanisms such as pain-related expectations or cognitions (pain-related fear, pain catastrophizing) modulate this effect. Two hundred forty-seven healthy participants underwent similar experimental paradigms, including a visual categorization task with images randomly paired with (or without) concomitant painful stimulation and a subsequent unannounced recognition task. Recognition memory (ie, d', recollection, and familiarity) and categorization performance (ie, reaction time, accuracy) served as proxies for the effect of pain on cognitive performance. Acute painful stimulation significantly impaired recognition performance (d', familiarity). However, recognition performance was not significantly modulated by participants' expectations regarding the effect of pain on task performance or pain-related cognitions in this sample of healthy participants. Our results corroborate the negative effects of pain on (visual) memory encoding reported in previous studies and reports of "memory problems" from patients with chronic pain. To characterize the role of bottom-up and top-down factors for the detrimental effects of pain, large-scale studies with more nuanced study designs are necessary. Future studies in patient cohorts must unravel the interaction of maladaptive pain-related cognitions and the often-reported impaired cognitive performance in chronic pain patients.

Neural mechanisms for secondary suppression of emotional distractors: Evidence from concurrent electroencephalography-magnetoencephalography data.

Distractor suppression allows us to remain on-task in the presence of distractions by filtering task-irrelevant information from ongoing cognitive processing and responding. Electrophysiological studies have revealed that this key feature of selective attention is a dynamic process that involves at least two distinct stages of processing. Two important aspects of these processing stages remain unclear: Whether the processing of emotional distractors at an earlier stage is automatic, as reflected in the N2/early posterior negativity (EPN) component; and what functional-anatomical brain systems are recruited in each stage. The present study addresses these issues by measuring brain activity with concurrent electroencephalography-magnetoencephalography (MEG) recordings while participants performed a combined rapid serial visual presentation and motion tracking task. Event-related potentials (ERP) showed significant effects of attentional capture and attentional modulation during two time windows marked by the N2/EPN and P3b ERP components. Source reconstruction of concurrent MEG measurements revealed activation of the left visual association cortex and anterior cingulate cortex during the N2/EPN time window, activation of the insula during the early phase of the P3b and anterior cingulate cortex activity during the later phase of the P3b. The findings provide novel evidence establishing a connection between the increased N2 response to negative pictures and the activation of the cingulate gyrus, which facilitates the suppression of distractions during demanding cognitive tasks. In addition, distinct activation patterns were observed in the insula and anterior cingulate cortex during the P3b time window, indicating that attentional control mediated by the anterior cingulate cortex operates to suppress the processing of distracting emotional stimuli. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Investigating links between Internet literacy, Internet use, and Internet addiction among Chinese youth and adolescents in the digital age.

In current digital era, adolescents' Internet use has increased exponentially, with the Internet playing a more and more important role in their education and entertainment. However, due to the ongoing cognitive, emotion, and social development processes, youth and adolescents are more vulnerable to Internet addiction. Attention has been paid to the increased use of Internet during the COVID-19 pandemic and the influence of Internet literacy in prevention and intervention of Internet addiction. The present study proposes a conceptual model to investigate the links between Internet literacy, Internet use of different purpose and duration, and Internet addiction among Chinese youth and adolescents. In this study, N = 2,276 adolescents studying in primary and secondary schools in East China were recruited, and they completed self-reports on sociodemographic characteristics, Internet literacy scale, Internet use, and Internet addiction scale. The results showed a significant relationship between Internet use and Internet addiction. To be specific, the duration of Internet use significantly and positively affected Internet addiction. With different dimensions of Internet literacy required, entertainment-oriented Internet use had positive impact on Internet addiction, while education-oriented Internet use exerted negative effects on Internet addiction. As for Internet literacy, knowledge and skills for Internet (positively) and Internet self-management (negatively) significantly influenced the likelihood of Internet addiction. The findings suggest that Internet overuse increases the risk of Internet addiction in youth and adolescents, while entertainment-oriented rather than education-oriented Internet use is addictive. The role of Internet literacy is complicated, with critical Internet literacy preventing the development of Internet addiction among youth and adolescents, while functional Internet literacy increasing the risk.

Long-term practice of intuitive inquiry meditation modulates EEG dynamics during self-schema processing

ObjectiveIntuitive inquiry meditation is a unique form of Buddhist Zen/Chan practice in which individuals actively and intuitively utilize the cognitive functions to cultivate doubt and explore the concept of the self. This event-related potential (ERP) study aimed to investigate the neural correlates by which long-term practice of intuitive inquiry meditation induces flexibility in self-schema processing, highlighting the role of doubt and belief processes in this exploration. MethodsTwenty experienced and eighteen beginner meditators in intuitive inquiry meditation were recruited for this ERP study. The interactions of doubt and belief processes with concepts of the self and Buddha were investigated. A 128-channel electroencephalography (EEG) system was used to collect EEG data. The ERP data were processed and analyzed using EEGLAB. ResultsThe data showed a double dissociation between beginners and experienced meditators (monks) in the concepts of the self and Buddha: intuitive inquiry meditation reduced the brain activity of beginners when viewing Buddha image but not when viewing a picture of themselves. However, in experienced meditators, intuitive inquiry meditation reduced brain activity when they viewed images of themselves but not when they viewed Buddha image. Further event-related spectral perturbation (ERSP) analysis revealed that experienced meditators had a greater theta spectral power and higher intertrial coherence (ITC), indicating that they could more flexibly modulate ongoing cognitive processes than beginner meditators. ConclusionIntuitive inquiry meditation could help beginner meditators detach from the concept of Buddha but not from that of the self. However, in experienced meditators, the opposite was true. ERSP analysis showed that only experienced meditators exhibited significant alterations in brain activity dynamics during intuitive inquiry meditation, which might enable these practitioners to become spontaneously detached from the concept of the self. These findings revealed the neural mechanism by which long-term practice of intuitive inquiry meditation can influence the doubting process and its effect on self-schema processing.

Tonic and phasic cardiac vagal activity predict cognitive-affective processing in an emotional stop-signal task

Both the Neurovisceral Integration Model and the Vagal Tank Theory propose cardiac vagal activity (CVA) as proxy for self-regulatory or processing resources required for attentional and top-down control as key mechanisms in executive functioning or emotion-regulation. These resources have, according to the Dual Competition Model, limited capacity and are prioritized for processing the most salient of different competing stimuli. As such, emotional and especially negative or threatening stimuli attract these self-regulatory/processing resources, and may thus interfere with ongoing cognitive processes if not integrated adaptively. Here, we investigated whether tonic and phasic CVA represent self-regulatory/processing resources and thus predict response inhibition in an emotional stop-signal task as a measure of cognitive-affective integration. Thereby, we examined not only the independent effects of tonic and phasic CVA, but whether and how the interaction of both variables predicts response inhibition. We assumed that the effects of CVA on response inhibition would be more pronounced in negatively-valenced trials compared to positive or neutral ones. Our results show that CVA significantly predicted response inhibition, with higher tonic CVA predicting faster, and larger phasic withdrawal CVA predicting slower stop-signal reaction times. The interactive effects of tonic and phasic CVA revealed that high tonic CVA levels prevented the negative effects of CVA withdrawal on response inhibition. Contrary to our assumption, we did not find differential effects of tonic and phasic CVA on trials with different valences. Our results support the notion of CVA as index for self-regulatory/processing resources. However, further research is needed to investigate how CVA is related to specific neural processes of cognitive-affective processing.

Extending the theoretical foundations of multimedia learning: Activation, integration, and validation occur when processing illustrated texts

BackgroundText illustrated by pictures (i.e., multimedia material) is often used to improve learning outcomes. To support learning, it is essential to understand and specify the ongoing cognitive processes when processing illustrated texts. AimsWe focus on three cognitive processes identified when processing non-illustrated texts: activation, integration, and validation. In three experiments, we investigated whether the three processes occur during the processing of illustrated texts and whether the processes differ between illustrated and non-illustrated texts. SamplesExperiment 1 had 170 participants, Experiment 2 had 221 participants, and Experiment 3 had 132 participants. MethodAll experiments used an adapted version of the contradiction paradigm. In Experiments 1 and 2, participants read texts that contained information that was consistent vs. inconsistent with a later sentence (target sentence). It was additionally varied whether a picture illustrated the consistent vs. inconsistent information. In Experiment 3, only the pictures were consistent or inconsistent with the target sentence. We measured reading times for the target sentence and the following sentence (spillover sentence). ResultsIn all three experiments, reading times were significantly longer in the inconsistent than in the consistent conditions. This prolongation of reading times was not affected by the picture in Experiments 1 and 2. ConclusionOur results indicate that activation, integration, and validation processes are similar when processing non-illustrated and illustrated texts (Experiments 1 and 2) and also occur when information is presented across text and picture (Experiment 3). We discuss the implications for the theoretical foundations of multimedia learning.

Cognitive influences on fixational eye movements

We perceive the world based on visual information acquired via oculomotor control,1 an activity intertwined with ongoing cognitive processes.2,3,4 Cognitive influences have been primarily studied in the context of macroscopic movements, like saccades and smooth pursuits. However, our eyes are never still, even during periods of fixation. One of the fixational eye movements, ocular drifts, shifts the stimulus over hundreds of receptors on the retina, a motion that has been argued to enhance the processing of spatial detail by translating spatial into temporal information.5 Despite their apparent randomness, ocular drifts are under neural control.6,7,8 However little is known about the control of drift beyond the brainstem circuitry of the vestibulo-ocular reflex.9,10 Here, we investigated the cognitive control of ocular drifts with a letter discrimination task. The experiment was designed to reveal open-loop effects, i.e., cognitive oculomotor control driven by specific prior knowledge of the task, independent of incoming sensory information. Open-loop influences were isolated by randomly presenting pure noise fields (no letters) while subjects engaged indiscriminating specific letter pairs. Our results show open-loop control of drift direction in human observers.

Pre-sleep arousal induced by suspenseful series and cliffhangers have only minor effects on sleep: A sleep laboratory study

Pre-sleep arousal impairs sleep. Therefore, watching suspenseful TV series before sleep is not recommended as they increase arousal. In particular, the consumption of multiple episodes of the same suspenseful TV series in one sitting – termed binge-watching – could lead to large increases in physiological arousal delaying sleep onset. Furthermore, abrupt endings during critical scenes – termed cliffhangers – result in unfinished story lines, which further increase cognitive arousal and could negatively impact sleep architecture and the number of awakenings. However, the effects of binge-watching and cliffhangers on objective sleep parameters are still unknown.Here we tested in a controlled sleep-laboratory setting whether pre-sleep arousal induced by watching 3–4 episodes of a suspenseful TV series has negative effects on sleep in 50 healthy young participants (39 females, mean age = 22.62 ± 2.60 (SD)). Watching a neutral TV series served as a control condition, according to a within-subject design. In one group of participants, the suspenseful TV series ended with a cliffhanger. In the other group, the same TV series ended where no ongoing action was interrupted. Pre-sleep arousal was measured both subjectively with the self-reported level of stress and objectively with the mean heart rate and cortisol level. As expected, suspenseful TV series induced higher cognitive and physiological pre-sleep arousal than neutral control TV series, with highest values for TV series with cliffhangers. In spite of the high pre-sleep arousal, participants fell asleep faster after watching the suspenseful compared with the neutral TV series (F(1,48) = 4.89, p = 0.032, η2 = 0.09). Sleep architecture and the number of awakenings remained unaffected. However, in the first two sleep cycles, heart rate was still higher after watching suspenseful TV series (F(1,48) = 6.76, p = 0.012, η2 = 0.12). And only after cliffhangers, objective sleep quality – measured as the ratio between slow-wave and beta activity during sleep – was lower than in the other conditions (interaction effect, F(1,48) = 5.05, p = 0.029, η2 = 0.10).Our results speak against large impairments of pre-sleep watching of multiple episodes of suspenseful TV series and cliffhangers on sleep quality and architecture. However, unfinished narratives might induce more subtle changes in oscillatory power during sleep, possibly reflecting ongoing cognitive processing during sleep.

Automatic Assessment of Procedural Skills Based on the Surgical Workflow Analysis Derived from Speech and Video.

Automatic surgical workflow analysis (SWA) plays an important role in the modelling of surgical processes. Current automatic approaches for SWA use videos (with accuracies varying from 0.8 and 0.9), but they do not incorporate speech (inherently linked to the ongoing cognitive process). The approach followed in this study uses both video and speech to classify the phases of laparoscopic cholecystectomy, based on neural networks and machine learning. The automatic application implemented in this study uses this information to calculate the total time spent in surgery, the time spent in each phase, the number of occurrences, the minimal, maximal and average time whenever there is more than one occurrence, the timeline of the surgery and the transition probability between phases. This information can be used as an assessment method for surgical procedural skills.

Oscillations without cortex: Working memory modulates brainwaves in the endbrain of crows.

Complex cognition requires coordinated neuronal activity at the network level. In mammals, this coordination results in distinct dynamics of local field potentials (LFP) central to many models of higher cognition. These models often implicitly assume a cortical organization. Higher associative regions of the brains of birds do not have cortical layering, yet single-cell correlates of higher cognition are very similar to those found in mammals. We recorded LFP in the avian equivalent of prefrontal cortex while crows performed a highly controlled and cognitively demanding working memory task. We found signatures in local field potentials, modulated by working memory. Frequencies of a narrow gamma and the beta band contained information about the location of target items and were modulated by working memory load. This indicates a critical involvement of these bands in ongoing cognitive processing. We also observed bursts in the beta and gamma frequencies, similar to those that play a vital part in 'activity silent' models of working memory. Thus, despite the lack of a cortical organization the avian associative pallium can create LFP signatures reminiscent of those observed in primates. This points towards a critical cognitive function of oscillatory dynamics evolved through convergence in species capable of complex cognition.

Divergences Between Resting State Networks and Meta-Analytic Maps Of Task-Evoked Brain Activity

Background: Spontaneous human neural activity is organized into resting state networks, complex patterns of synchronized activity that account for the major part of brain metabolism. The correspondence between these patterns and those elicited by the performance of cognitive tasks would suggest that spontaneous brain activity originates from the stream of ongoing cognitive processing. Objective: To investigate a large number of meta-analytic activation maps obtained from Neurosynth (www.neurosynth.org), establishing the extent of task-rest similarity in large-scale human brain activity. Methods: We applied a hierarchical module detection algorithm to the Neurosynth activation map similarity network, and then compared the average activation maps for each module with a set of resting state networks by means of spatial correlations. Results: We found that the correspondence between resting state networks and task-evoked activity tended to hold only for the largest spatial scales. We also established that this correspondence could be biased by the inclusion of maps related to neuroanatomical terms in the database (e.g. “parietal”, “occipital”, “cingulate”, etc.). Conclusion: Our results establish divergences between brain activity patterns related to spontaneous cognition and the spatial configuration of RSN, suggesting that anatomically-constrained homeostatic processes could play an important role in the inception and shaping of human resting state activity fluctuations.

Prominent gamma band activity during visual motion perception in early-stage Alzheimer's disease.

IntroductionAlzheimer’s disease (AD) affects multiple neural pathways and regions, resulting in various visual impairments such as motion perception. Generally, gamma-band activities during visual motion perception have been thought to reflect ongoing cognitive processes. Nevertheless, few studies have specifically examined induced gamma band activity during visual motion perception in AD patients. Therefore, after performing magnetoencephalography (MEG) recording during apparent motion (AM) stimulation for the left hemi-visual field in patients diagnosed as having AD in the early stage, we compared the results with findings of cognitive performance.MethodsSeventeen AD patients in the early stage and 17 controls matched for age, sex, and educational attainment participated in this study. For each participant, memory performance was assessed with the Mini-Mental State Examination (MMSE) and the Wechsler Memory Scale-Revised (WMS-R). For MEG analysis, we examined power changes induced in a higher frequency range (20–100 Hz) after AM stimuli.ResultsThe power of induced gamma band activities was significantly higher in AD patients. The power of induced gamma band activities was associated with higher performance on both MMSE and WMS-R tests for attention and concentration in AD patients.ConclusionsGiven that neuronal dysfunction in AD is associated with excitotoxic neurodegeneration, and given that subsequent development of compensatory inhibitory mechanisms also contributes to pathology in AD patients, elevated gamma band oscillations might reflect an imbalance of inhibitory and excitatory activity in AD patients. Moreover, positive correlation between induced gamma activity and cognitive performance might signify a compensating mechanism of inhibitory neurons which preserve the pyramidal neuron from excitotoxicity in a posterior association area.

Happy facial expressions impair inhibitory control with respect to fearful facial expressions but only when task-relevant.

The ability to generate appropriate responses, especially in social contexts, requires integrating emotional information with ongoing cognitive processes. In particular, inhibitory control plays a crucial role in social interactions, preventing the execution of impulsive and inappropriate actions. In this study, we focused on the impact of facial emotional expressions on inhibition. Research in this field has provided highly mixed results. In our view, a crucial factor explaining such inconsistencies is the task-relevance of the emotional content of the stimuli. To clarify this issue, we gave two versions of a Go/No-go task to healthy participants. In the emotional version, participants had to withhold a reaching movement at the presentation of emotional facial expressions (fearful or happy) and move when neutral faces were shown. The same pictures were displayed in the other version, but participants had to act according to the actor's gender, ignoring the emotional valence of the faces. We found that happy expressions impaired inhibitory control with respect to fearful expressions, but only when they were relevant to the participants' goal. We interpret these results as suggesting that facial emotions do not influence behavioral responses automatically. They would instead do so only when they are intrinsically germane for ongoing goals. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Working Memory in People with Schizophrenia.

Working memory (WM) refers to the ability to maintain a small number of representations in an activated, easily accessible state for a short period of time in the service of ongoing cognitive processing and behavior. Because WM is a resource critical for multiple forms of complex cognition and executive control of behavior, it is of central interest in the study of disorders such as schizophrenia that involve a broad compromise of cognitive function and in the regulation of goal-directed behavior. There is now robust evidence that WM impairment is characteristic of people with schizophrenia. The impairment includes both elementary storage capacity as well as more complex forms of WM that involve the manipulation and updating of WM representations. These impairments appear to underlie a substantial portion of the generalized cognitive deficit in schizophrenia. Neuroimaging studies have implicated widespread abnormalities in the broad neural system that subserves WM performance, consistent with the evidence of broad cognitive impairment seen in PSZ.

Differentiating global luminance, arousal and cognitive signals on pupil size and microsaccades.

Pupil size reflects a proxy for neural activity associated with global luminance, arousal and cognitive processing. Microsaccades are also modulated by arousal and cognitive processing. Are these effects of arousal and cognitive signals on pupil size and microsaccades coordinated? If so, via what neural mechanisms? We hypothesized that if pupil size and microsaccades are coordinately modulated by these processes, pupil size immediately before microsaccade onset, as an index for ongoing cognitive and arousal processing, should correlate with microsaccade responses during tasks alternating these signals. Here, we examined the relationship between pupil size and microsaccade responses in tasks that included variations in global luminance, arousal and inhibitory control. Higher microsaccade peak velocities correlated with larger pre-microsaccade pupil response related to arousal and inhibitory control signals. In contrast, pupil responses evoked by global luminance signals did not correlate with microsaccade responses. Given the central role of the superior colliculus in microsaccade generation, these results suggest the critical involvement of the superior colliculus to coordinate pupil and microsaccade responses for arousal and inhibitory control modulations, but not for the pupil luminance modulation.

Analysis of Data-Based Scientific Reasoning from a Product-Based and a Process-Based Perspective

In this study, we investigated participants’ reactions to supportive and anomalous data in the context of population dynamics. Based on previous findings on conceptions about ecosystems and responses to anomalous data, we assumed a tendency to confirm the initial prediction after dealing with contradicting data. Our aim was to integrate a product-based analysis, operationalized as prediction group changes with process-based analyses of individual data-based scientific reasoning processes to gain a deeper insight into the ongoing cognitive processes. Based on a theoretical framework describing a data-based scientific reasoning process, we developed an instrument assessing initial and subsequent predictions, confidence change toward these predictions, and the subprocesses data appraisal, data explanation, and data interpretation. We analyzed the data of twenty pre-service biology teachers applying a mixed-methods approach. Our results show that participants tend to maintain their initial prediction fully or change to predictions associated with a mix of different conceptions. Maintenance was observed even if most participants were able to use sophisticated conceptual knowledge during their processes of data-based scientific reasoning. Furthermore, our findings implicate the role of confidence changes and the influences of test wiseness.

Does pain modality play a role in the interruptive function of acute visceral compared with somatic pain?

Acute pain captures attentional resources and interferes with ongoing cognitive processes, including memory encoding. Despite broad clinical implications of this interruptive function of pain for the pathophysiology and treatment of chronic pain conditions, existing knowledge exclusively relies on studies using somatic pain models. Visceral pain is highly prevalent and seems to be more salient and threatening, suggesting that the interruptive function of pain may be higher in acute visceral compared with somatic pain. Implementing rectal distensions as a clinically relevant experimental model of visceral pain along with thermal cutaneous pain for the somatic modality, we herein examined the impact of pain modality on visual processing and memory performance in a visual encoding and recognition task and explored the modulatory role of pain-related fear and expectation in 30 healthy participants. Despite careful and dynamically adjusted matching of stimulus intensities to perceived pain unpleasantness over the course of trials, we observed greater impairment of cognition performance for the visceral modality with a medium effect size. Task performance was not modulated by expectations or by pain-related fear. Hence, even at matched unpleasantness levels, acute visceral pain is capable of interfering with memory encoding, and this impact seems to be relatively independent of pain-related cognitions or emotions, at least in healthy individuals. These results likely underestimate the detrimental effect of chronic pain on cognitive performance, which may be particularly pronounced in acute and chronic visceral pain.

Neuroanatomical correlates of self-awareness of highly practiced visuomotor skills.

Metacognition is the ability to introspect and control ongoing cognitive processes. Despite the extensive investigation of the brain architectures supporting metacognition for perception and memory, little is known about the neural basis of metacognitive capacity for motor function, a vital aspect of human behavior. Here, using functional and structural magnetic resonance imaging (MRI), we examined the brain substrates underlying self-awareness of handwriting, a highly practiced visuomotor skill. Results showed that experienced adult writers generally overestimated their handwriting quality, and such overestimation was more pronounced in men relative to women. Individual variations in self-awareness of handwriting quality were positively correlated with gray matter volume in the left fusiform gyrus, right middle frontal gyrus and right precuneus. The left fusiform gyrus and right middle frontal gyrus are thought to represent domain-specific brain mechanisms for handwriting self-awareness, while the right precuneus that has been reported in other domains likely represents a domain-general brain mechanism for metacognition. Furthermore, the activity of these structurally related regions in a handwriting task was not correlated with self-awareness of handwriting, suggesting the correlation with metacognition was independent of task performance. Together, this study reveals that metacognition for practiced motor skills relies on both domain-general and domain-specific brain systems, extending our understanding about the neural basis of human metacognition.

Dividing attention impairs metacognitive control more than monitoring.

Students consistently report multitasking (e.g., checking social media, texting, watching Netflix) when studying on their own (e.g., Junco & Cotton, Computers & Education, 59[2], 505–514, 2012). Multitasking impairs explicit learning (e.g., Carrier, Rosen, Cheever, & Lim, Developmental Review, 35, 64–78, 2015), but the impact of multitasking on metacognitive monitoring and control is less clear. Metacognition may compete with ongoing cognitive processing for mental resources (e.g., Nelson & Narens, The Psychology of Learning and Motivation, 26, 125–141, 1990) and would be impaired by dividing attention; alternatively, metacognition may require little attention (e.g., Boekaerts & Niemivirta, Handbook of Self-Regulation [pp. 417–450], 2000) and would not be impacted by dividing attention. Across three experiments, we assessed the influence of divided attention on metacognition. Participants made item-by-item judgements of learning (JOLs) after studying word pairs under full or divided attention (Experiment 1) and made restudy choices (Experiments 2 & 3). Dividing attention had little impact on the resolution of learners’ metacognitive monitoring, but significantly impaired calibration of monitoring, the relationship between monitoring and control, and the efficacy of metacognitive control. The data suggest that monitoring may require few cognitive resources, but controlling one’s learning (e.g., planning what to restudy and implementing a plan) may demand significant mental resources.Supplementary InformationThe online version contains supplementary material available at 10.3758/s13423-021-01950-9.

Multivariate analysis reveals a generalizable human electrophysiological signature of working memory load.

Working memory (WM) is an online memory system that is critical for holding information in a rapidly accessible state during ongoing cognitive processing. Thus, there is strong value in methods that provide a temporally resolved index of WM load. While univariate EEG signals have been identified that vary with WM load, recent advances in multivariate analytic approaches suggest that there may be rich sources of information that do not generate reliable univariate signatures. Here, using data from four published studies (n=286 and>250,000 trials), we demonstrate that multivariate analysis of EEG voltage topography provides a sensitive index of the number of items stored in WM that generalizes to novel human observers. Moreover, multivariate load detection ("mvLoad") can provide robust information at the single-trial level, exceeding the sensitivity of extant univariate approaches. We show that this method tracks WM load in a manner that is (1) independent of the spatial position of the memoranda, (2) precise enough to differentiate item-by-item increments in the number of stored items, (3) generalizable across distinct tasks and stimulus displays, and (4) correlated with individual differences in WM behavior. Thus, this approach provides a powerful complement to univariate analytic approaches, enabling temporally resolved tracking of online memory storage in humans.