Abstract. This study aims at introducing two conservative thermodynamic variables (moist-air entropy potential temperature and total water content) into a one-dimensional variational data assimilation system (1D-Var) to demonstrate their benefits for use in future operational assimilation schemes. This system is assessed using microwave brightness temperatures (TBs) from a ground-based radiometer installed during the SOFOG3D field campaign, dedicated to fog forecast improvement. An underlying objective is to ease the specification of background error covariance matrices that are highly dependent on weather conditions when using classical variables, making difficult the optimal retrievals of cloud and thermodynamic properties during fog conditions. Background error covariance matrices for these new conservative variables have thus been computed by an ensemble approach based on the French convective scale model AROME, for both all-weather and fog conditions. A first result shows that the use of these matrices for the new variables reduces some dependencies on the meteorological conditions (diurnal cycle, presence or not of clouds) compared to typical variables (temperature, specific humidity). Then, two 1D-Var experiments (classical vs. conservative variables) are evaluated over a full diurnal cycle characterized by a stratus-evolving radiative fog situation, using hourly TB. Results show, as expected, that TBs analysed by the 1D-Var are much closer to the observed ones than the background values for both variable choices. This is especially the case for channels sensitive to water vapour and liquid water. On the other hand, analysis increments in model space (water vapour, liquid water) show significant differences between the two sets of variables.