Background: Generalized Anxiety Disorder (GAD) is a mental illness that significantly affects various domains of daily functioning. Limited research has been conducted on GAD among medical students in Sudan, particularly during the socio-political and economic crises. This study aimed to assess the prevalence of GAD, identify risk factors, and evaluate its impact on academic performance and daily activities among Sudanese medical students. Methods: A cross-sectional study was conducted among undergraduate medical students at Omdurman Islamic University. Data were collected using a self-administered online questionnaire via Google Forms, consisting of two parts: socio-demographic information and the Generalized Anxiety Disorder Questionnaire (GAD-7), a validated tool for screening and measuring the severity of GAD. Results: A total of 374 medical students participated, with 64.7% being female. The GAD-7 scores were high (above 9), suggesting GAD among 33.7% of participants, with severity levels of 41.2% for mild anxiety, 21.4% for moderate anxiety, and 12.3% for severe anxiety. Comparison of means showed significant associations between GAD and female students (p<0.001) and students with chronic diseases (p=0.034). GAD significantly impacted daily activities (p<0.001). Multiple logistic regression analysis found that students in the final year had significantly higher GAD-7 scores (Adjusted Odds Ratio=4.25, 95% Confidence Interval=1.27-14.22). Conclusions: The higher scores on the GAD-7 measure among Sudanese medical students are concerning. This emphasizes the urgent need to raise awareness, normalize mental health discussions, and provide accessible counseling services tailored to the students' needs.