Edible Oils Research Articles

The memory-improving effect of Hizikia forsiforme functional oil microcapsule (HFFOM) prepared by subcritical extraction and complex coacervational embedding

Functional foods are prospective in improving memory decline caused by Alzheimer’s disease and aging. The Edible seaweed Hizikia fusiforme functional oil (HFFO) inhibited acetylcholinesterase, prevented cellular neuroinflammation, and improved memory deficits in zebrafish models in the previous studies. To realize its greener preparation and lipid oxidation mitigation and study its effects on higher animals, more investigations are necessary. Herein, sub-critical fluid extraction of HFFO and successive complex coacervation embedding yielded a water-soluble and anti-oxidative microcapsule HFFOM, which displayed in vivo memory-improving effects on LPS-injured zebrafish and normal rodents in different behavior tests. HFFOM can elevate the level of the neurotransmitter acetylcholine while decreasing the levels of MDA, IL-1β, and TNF-α in the zebrafish brain. LC-MS/MS and lipidomic analysis suggested its predominant memory-improving ingredients to be linolenic, erucic, nervonic, arachidonic, linoleic, eicosatrienoic, docosahexaenoic, and docosapentaenoic acids. This study enlightens the potential of HFFOM in developing memory-enhancing functional foods or drugs.

Brassinosteroids: An Innovative Compound Family That Could Affect the Growth, Ripening, Quality, and Postharvest Storage of Fleshy Fruits

Brassinosteroids (BRs), a new family of plant hormones, have been used in a range of food staples, oil crops, and cereals. However, the scientific literature pertaining to their use in fleshy fruits remains scarce. This review presents, for the first time, the knowledge developed over the last decade on the role of BR preharvest treatments in crop yield and fruit quality properties at harvest and during storage, although information about the effects of BR postharvest treatments is also addressed. This review revealed that 24-epibrassinolide is the most used BR analogue in research experiments, either as a pre- or postharvest treatment, with doses ranging from 0.1 to 15 μM. Additionally, most of the research has been conducted on non-climacteric fruit species. In most of these preharvest treatments, an increase in crop yield has been reported, as well as enhanced anthocyanin concentration in red-coloured fruit. In addition, increases in firmness, total soluble solids, and phenolic content have also been observed. On the other hand, BR postharvest treatments led to the maintenance of these fruit quality properties during storage due to increased antioxidant systems, either enzymatic or non-enzymatic ones. Finally, as future perspectives, it is proposed to extend the research about BR treatments to other climacteric fruits and to deepen the knowledge of how BRs regulate physiological aspects from preharvest to postharvest. Furthermore, it is essential to investigate the role of BRs in the prevention of rot and biotic stress.

Eco-Friendly Detergent Based on Exhausted Edible Vegetable Oils: Impact on Marine and Freshwater Environments, a Case Study Focusing on SARS-CoV-2

Eco-Friendly Detergent Based on Exhausted Edible Vegetable Oils: Impact on Marine and Freshwater Environments, a Case Study Focusing on SARS-CoV-2

Fatty acid profiling of bottle gourd [<i>Lagenaria siceraria </i>(Mol.) Standl.] seed oil using gas chromatography-mass spectrometry

The present investigation was carried out to study the oil quality and estimate fatty acid (omega-3 and omega- 6) composition in bottle gourd using gas chromatography-mass spectrometry. Results indicated that the extracted bottle gourd seed oil was estimated to have an oil content (25.2%), polyunsaturated fatty acids viz., omega-3 (6.63%), omega-6 (69.74%) and others (0.17%), which play a major role in, cardiovascular health and has anti-inflammatory, anti-allergic and anti-cancer benefits. It also consisted of saturated fatty acids viz., palmitic acid (9.78%), stearic acid (4.45%) and others (0.97%) along with monounsaturated fatty acids (MUFA) (5.99%) without oleic acid (omega-9). Due to increased demand for specialty edible oil in both domestic and industrial use, it becomes necessary to exploit the potential of bottle gourd seeds as a novel source of edible oil. Being a rich source of omega-6 and omega-3 fatty acids, which lacks omega-9 known for oxidative stability required for storage and cooking, it can be commercialized as cooking oil by blending it with edible oil rich in oleic acid or can also be supplied as an omega-6-rich capsule as a food supplement in future.

Evaluation of Fuel Consumption and Exhaust Emissions in a Single Cylinder Four-Stroke Diesel Engine Using Biodiesel Derived from Chicken Waste with Additives

Biodiesel is a renewable, locally generated fuel made from vegetable oils, trans fats, or recycled restaurant grease for use in diesel automobiles and other diesel-powered equipment. The physical qualities of biodiesel are comparable to those of petroleum diesel. Biodiesel is used in compression-ignition engines in the same way as petroleum diesel. Biofuel can be made from biomass, but according to the Indian National Policy on Biofuels, biofuel extraction from edible oils is not permitted because it would result in a massive increase in consumer goods prices and perhaps lead to product scarcity. Hence, there is a need to search for alternative biofuel so that it does not affect our nation's national policy. Recently there has been a drastic increase in the consumption of chicken grown in farms. There is a huge problem in disposing of their waste. One major difficulty in waste treatment is poultry manure, often known as slaughter waste. This waste is sometimes dumped on wastelands, roadsides, and the banks of rivers, lakes, and ponds. This creates more sanitary problems for society. According to polls, poultry feces begin to decompose in around three hours. The odor of butcher's waste causes a lot of pollution in the air, as well as an increase in the density of bacteria in the atmosphere. Measures have been taken by the government for the safe disposal of these wastes. One of the major ways of disposal is to recycle this chicken slaughter waste in a useful manner so that it can serve society in a positive way. This could be done by converting this waste into fuel through various processes such as rendering, hydrolysis, extraction, acid esterification, alkaline transesterification, and finally by the extraction process. This produced fuel could be blended with existing diesel in varying concentrations and used in diesel engine-powered vehicles. In this work, the fuel is extracted from the chicken waste and blended with pure diesel and an Al2O3 additive in various proportions. These combinations are then tested on a single-cylinder diesel engine for fuel consumption and emission analysis. The results exhibited an overall better performance when compared to pure diesel.

Perspective on the health effects of unsaturated fatty acids and commonly consumed plant oils high in unsaturated fat.

Epidemiological and clinical trial evidence indicates that n-6 polyunsaturated fatty acid (PUFA) intake is cardioprotective. Nevertheless, claims that n-6 PUFA intake promotes inflammation and oxidative stress prevail. This narrative review aims to provide health professionals with an up-to-date evidence overview to provide the requisite background to address patient/client concerns about oils containing predominantly unsaturated fatty acids (UFA), including MUFA and PUFA. Edible plant oils, commonly termed vegetable oils, are derived from vegetables, nuts, seeds, fruits and cereal grains. Substantial variation exists in the fatty acid composition of these oils; however, all are high in UFA, while being relatively low in saturated fatty acids (SFA), except for tropical oils. Epidemiological evidence indicates that higher PUFA intake is associated with lower risk of incident CVD and type 2 diabetes mellitus (T2DM). Additionally, replacement of SFA with PUFA is associated with reduced risk of CVD and T2DM. Clinical trials show higher intake of UFA from plant sources improves major CVD risk factors, including reducing levels of atherogenic lipids and lipoproteins. Importantly, clinical trials show that increased n-6 PUFA (linoleic acid) intake does not increase markers of inflammation or oxidative stress. Evidence-based guidelines from authoritative health and scientific organisations recommend intake of non-tropical vegetable oils, which contain MUFA and n-6 PUFA, as part of healthful dietary patterns. Specifically, vegetable oils rich in UFA should be consumed instead of rich sources of SFA, including butter, tallow, lard, palm and coconut oils.

Characterization of quality parameters and phytosterol content in oils and their formulated margarines

AbstractUnlike lipid stability and oxidation studies in commonly used edible oils and margarines, margarines formulated with unconventional oils are not well characterized. This study investigated the effect of heat treatment (HT) on the stability and content of phytosterol in njangsa seed oil (NSO), bush mango oil (BMO), soybean oil (SBO), coconut oil (CCO), and margarines formulated from their blends: BN (BMO and NSO), BS (BMO and SBO), CN (CCO and NSO), CS (CCO and SBO), and commercial margarines (CM1 and CM2). Both oils and margarines were heat‐treated at 130, 170, and 210°C for 10, 15, 20, and 120 min (only oils). Changes in free fatty acid (FFA), peroxide value (PV), para‐anisidine value (AnV), and fatty acid (FA) composition and phytosterol content were determined after 20 min (margarines) and 120 min (oils) of HT and compared to their control/pre‐HT/unheated (UH) counterparts. The FA composition did not change significantly with HT. Polyunsaturated fatty acids (PUFA)‐rich oils such as NSO and SBO showed significantly higher increase in FFA content with HT compared with oils with higher saturated fatty acid content (BMO and CCO). Oils with higher proportions of linoleic acid, such as SBO (68.3%) and NSO (60.35%), had higher AnV at the end of the HT compared with oils with lower content, such as BMO (0.51%). Phytosterol content fluctuated with HT, and changes in content were generally more pronounced in β‐sitosterol than in stigmasterol and campesterol. Both principal component analysis (PCA) and partial least‐squares discriminant analysis (PLS‐DA) were carried out to observe possible clusters. The results suggest that changes in quality and content of oils and margarines during heating are dependent on more than their fatty acid composition.

Ensuring Food Safety through Effective Supply Chain Management:Challenges and Suggestions

Ensuring food safety is a complex challenge exacerbated by the global nature of modern supply chains. Effective supply chain management plays a crucial role in safeguarding food. This paper explores the concerns of food safety that causes by unethical food supply chain. Case studies from both developed and developing regions illustrate successful initiatives and highlight lessons learned. The role of collaboration among stakeholders—producers, processors, distributors, regulators, and consumers—is emphasized as crucial for effectively managing risks and ensuring compliance with safety standards throughout the supply chain. This essay examines how supply chain issues affect food safety through three case studies: China’s oil scandal mixing edible and chemical oils, the 2022 U.S. peanut butter recall due to salmonella contamination, and antibiotic-contaminated Vietnamese shrimp amid Japan’s nuclear water release. Each case underscores the impact on public health and trust. By examining three current cases, this paper aims to provide a comprehensive understanding of how ineffective supply chain management can create food safety risks and reduce consumer confidence in the safety and integrity of the food supply chain and suggest possible course of action.

Detection of Tert-Butylhydroquinone in Edible Oils Using an Electrochemical Sensor Based on a Nickel-Aluminum Layered Double Hydroxide@Carbon Spheres-Derived Carbon Composite

Phenolic antioxidants such as tert-butylhydroquinone (TBHQ) can prolong the shelf life of edible oils by delaying the oxidation process. The excessive use of TBHQ can damage food quality and public health, so it is necessary to develop an efficient TBHQ detection technique. In this work, nickel-aluminum double hydroxide (NiAl-LDH) was grown on glucose carbon spheres (GC), which formed porous carbon nanomaterials (named NiAl-LDH@GC-800) after pyrolysis at 800 °C. The successful synthesis of the material was verified by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The obtained NiAl-LDH@GC-800 was dopped onto a glass carbon electrode to prepare an electrochemical sensor for TBHQ. The synergistic effect of porous carbon and Ni metal reduced from NiAl-LDH by high-temperature calcination accelerated the electron transfer rate and improved the sensitivity of the sensor. The prepared sensor showed a low limit of detection (LOD) of 8.2 nM, a high sensitivity (4.2 A·M−1), and a good linear range (20~300 µM) in detecting TBHQ. The sensor was also successfully used for TBHQ detection in edible oils, including chili oil, peanut oil, and rapeseed oil.

The Influence of the Type and Concentration of Plasticizer on the Properties of Biopolymer Films based on Wild Flax (Camelina Sativa)

The development of biodegradable packaging materials using naturally occurring, renewable biopolymers has gained attraction due to consumer demand for high-quality products and concerns about environmental waste problems. However, the inferior mechanical properties and low water resistance of packaging materials based on natural polymers pose a significant obstacle to their wider use. One of the ways to improve the properties of biopolymer-based packaging materials is the addition of plasticizers during their synthesis. In this work, the influence of the type and concentration of plasticizer on the properties of new biopolymer films based on wild flax (Camelina sativa) was investigated. Camelina sativa oil cake (CSoC) remains after edible oil cold pressing as a by-product. One of the possibilities for its valorization is the synthesis of biopolymer materials. During the synthesis, different plasticizers - glycerol and polyethylene glycol 400 - were added in different concentrations - 20%-60%. The obtained CSoC-based biopolymer films were analyzed for the following properties: Moisture content, solubility, thickness, tensile strength, elongation at break, and water vapor permeability. The results obtained showed significant differences when different plasticizers were applied at different concentrations. The biopolymer film with optimal properties was obtained by adding glycerol at a concentration of 40%.

Comparative analysis of the physicochemical properties and trace metal content of palm oil, from selected markets in Jos south and Jos north LGA, Plateau state, Nigeria

The quality of palm oil samples collected from three different markets in Jos South and Jos North Local Government Area of Plateau State were analyzed and evaluated using standard analytical laboratory procedures. The relative ranges of the physicochemical values obtained were as follows; Moisture Content (MC): 0.56 - 1.89, Iodine value (IV); 51.88 - 55.57 g I2/100g, Peroxide Value (PV): 0.98 - 4.16 mEq02/kg, Saponification Values (SV): 198.46 - 201.54 mgKOH/g, Acid Value (AV): 9.93 - 57.65, and Refractive Index (RI), 1.4576 - 1.4580. The moisture content, iodin value, pH values and acid values were found to exceed the Standard Organization of Nigeria (SON) permissible limits of moisture content (0.29 mgKOH/g), iodine value (45 - 53 mg I2/100g), pH (5 - 7 mg/L), and acid value (0.7 max mg/L) for edible oils respectively while the refractive index, saponification values, peroxide values falls within the SON allowable limits of refractive index (1.4612-1.4707 mg/L), saponification value (195-205 mgKOH/g) and peroxide value (10 mEq02/kg) respectively . The amounts of trace metals (Cu, Zn, Fe, Pb and Cd) determined in the palm oil using the atomic absorption spectrophotometer ranged from Cu(0.34 - 0.52 mg/l), Zn(1.58 - 2.33 mg/l), Fe(19.18 - 26.74 mg/l), Pb(2.87 - 4.06 mg/l) and Cd(1.58 - 2.24 mg/l). However, the levels of Zn, Fe, Pb and Cd exceeded the WHO/FAO recommended values of (1.50, 1.00, 0.05 and 0.02 mg/L) respectively while only Cu concentration was below the WHO/FAO limits of 2.00 mg/L for trace metals in edible palm oil. Consequently, the results of this study shows that the palm oil consumed from these selected markets does not meet the standard quality specification for edible palm oils and hence can post significant health threat to consumers. Therefore, food regulatory agencies should strengthen their surveillance to promote food and health safety of the populace.

Design & development of adulteration detection system by fumigation method & machine learning techniques

A novel method for discovery of adulteration in edible oil is proposed based on concept of refractive index and electronic sensors. The research work focusses on two distinct methodologies like employing datasets and implementing a fumigation technique that integrates real-time hardware for testing Edible oil Impurities. In the first method, the dataset taken into consideration contains spectral data collected using Advanced ATR-MIR Spectroscopy for pure oil and various levels of adulteration with Vegetable oil. Each and every edible oil has a certain value of refractive index. When such oils are contemned in a change adding adulterants, the value of its refractive indices also changes. This value of refractive index serves as a feature for testing the oil and helps us in detecting the adulteration. If Oil is adulterated with vegetable oils, the refractive index will be lower and with animal fats, the refractive index will be higher than that of pure Oil. While in Fumigation Method a hardware module is develop in which adulterated & pure oil samples are heated at 40–50 °C for 4.66 min and the volatiles that are generated by varying gas concentrations are forcefully passed through to the MEMS Gas Sensor-MISC-2714 and Multichannel Gas sensor. The conductance of the sensors changes according to the gases sensed by the sensors contributes to features extraction. The conductance value serves as a feature for the classifier to determine whether the sample is highly, moderately, or lowly contaminated. Thus, in proposed methods we use different algorithms based on machine learning like KNN, Random Forest, CATBOOST and XGBOOST to accurately reveal the adulteration. Amongst all the applied algorithm Random Forest (RF) Classifier & XGBOOST algorithm outperform well and gives 100% accuracy. The proposed work is used for identifying food adulteration in edible food products which helps us to feed Society with high-quality food.

Development of Oil Industry in Poland in the Context of the European Union

Oil industry is developing well in Poland. This study aimed to examine the development of the Polish oil industry after joining the European Union. The following questions were asked: What were the changes in the consumption of vegetable oils in Poland after EU enlargement in 2004? How did the production of vegetable oil change in Poland after EU enlargement in 2004? And has the liquidity of the Polish oil industry changed after EU enlargement in 2004? First, the changes in the oil industry were evaluated. This research proved the development of the fat industry. We used the regression model to analyze the impact of chosen factors on the economic situation of the oil industry in Poland. Rapeseed is the main source of oil for the edible oil and biodiesel industry. High prices of rapeseed increased the sown area of rapeseed. The production of crude rapeseed oil changed from 520.1 thousand tons in 2005 to 1440 thousand tons in 2023. Biofuels made from rapeseed oils are called first generation. Poland’s production of refined rapeseed oil changed from 386.5 thousand tons in 2005 to 541 thousand tons in 2023. These numbers confirm the development of edible oil and biofuel production. The gross profit increased from PLN 133 mln in 2005 to PLN 443.8 mln in 2023. The net profit of the fat industry increased from PLN 110 mln in 2005 to PLN 381.6 mln in 2023. The oil industry is characterized by high investment, which reached the level of PLN 232.9 mln in 2023. The investment rate in 2023 was PLN 1.96 %. The changes observed in the rapeseed market after Poland joined the EU affected the performance of vegetable oil and biofuel producers. The regression analysis proved the hypothesis that the monthly consumption of rapeseed oil is the main factor influencing the oil industry situation in Poland. The oil industry is important to the development of the whole economy. Particular attention should be paid to current liquidity, which is why a constant supply of money from sold oil and biodiesel is needed to cover costs.

Comparative study for the nonlinear properties of edible vegetable oils by using Z-scan technique

Comparative study for the nonlinear properties of edible vegetable oils by using Z-scan technique

Comparative study of volatile compounds of cold-pressed oils extracted from three different oilseeds after gamma irradiation.

To investigate the effect of gamma irradiation on the volatile compounds of edible oils. Three types of oilseeds, including peanut, sesame, and flaxseed, were subjected to 8 kGy gamma irradiation, followed by cold pressing to extract their oils. The volatile compounds of the oils were isolated by simultaneous distillation extraction and analyzed by gas chromatography-mass spectrometry. A total of 91 volatile compounds were identified, which can be grouped into eight categories: hydrocarbons, aldehydes, ketones, alcohols, acids, esters, furans, and benzene derivatives. Irradiation treatment resulted in a significant increase in the levels of hydrocarbons, aldehydes, and ketones in all oil samples (p<0.05), with the greatest increase observed in hydrocarbons (4-14 times). In contrast, changes in alcohols, acids, esters, furans, and benzene derivatives were related to oilseed type. The increased hydrocarbons mainly originated from the degradation of palmitic, stearic, oleic, and linoleic acids. The irradiation resistance of the three oilseeds varied considerably, in the order: flaxseed>sesame>peanut. Based on the odor activity value, 11 key aroma compounds were selected, and (E)-2-decenal (tallow, oily, and orange), 1-octanol (soapy and oily), and 1-nonanol (floral and soapy) were only detected in the irradiated samples. Principal component analysis revealed that the oil samples of the three oilseeds could be well classified based on their key aroma compounds, and that the irradiation treatment had no remarkable effect on their intrinsic aroma.

Bioactive compounds delivery and bioavailability in structured edible oils systems.

The health benefits of bioactive compounds are dependent on the amount of intake as well as on the amount of these compounds that become bioavailable and bioaccessible. Various systems have been developed to deliver and increase the bioaccessibility of bioactive compounds. This review explores the impact of gelled (oleogels, bigels, emulgels, emulsions, hydrogels, and hydrogel beads), micro-(gels, particles, spheres, capsules, emulsions, and solid lipid microparticles) and nanoencapsulated systems (nanoparticles, solid lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, liposomes, and nanoliposomes) on the digestibility and bioavailability of lipophilic and hydrophilic bioactives. Structurant molecules, the oil type, antioxidants, emulsifiers, and coatings in delivery systems with promising potential in food applications are critically discussed. The release and bio-accessibility of bioactive compounds in gelled systems are influenced by various factors, such as the type and concentration of gelators, the gelator-to-oil ratio, the type of antioxidant, the network of the system, and its hydrophobicity. The stability, bioaccessibility, and controlled release of bioactives were improved in structured emulsions. Several variables, including wall material, oil/water ratios, encapsulation process, and pH conditions, can affect the bioactives release in microencapsulated systems. Factors like coating type and core-to-wall ratio impact the stability and release of core components. The encapsulating material, the encapsulation technology, and the nature of the nanomaterials all have an impact on the bioaccessibility of nanoencapsulated systems. Nanoliposomes provide enhanced stability and absorption. In general, all encapsulated systems have shown great potential in improving the distribution and availability of bioactive compounds.

Palm Oil Crisis in India's FMCG Sector – A Sustainability Dilemma

The FMCG companies in India and world over relies heavily on Palm oil as one of the core ingredients is going through difficult times. Oil Palm plantations have resulted in phenomenal ecological damage and biodiversity in southeast Asian countries which played a significant role in socio-economic development of the local community earlier. The supply chain disruptions along with pressing environmental or social concerns have negatively affected the balance sheets of Indian FMCG companies who was already under the strain of foreign currency fluctuations due to high import dependency. The government has initiated multiple programs under National Mission on Edible Oil to develop prospective solutions to resolve the crisis through sustainable palm oil agricultural practices as supporting environmental norms yet profitable outlook for self-consumption, leveraging government subsidies, inputs and technology through research and knowledge transfer. The study suggests the best competitive strategic measures which can be adopted.

Effect of Dietary Benzoic Acid and Oregano Essential Oil as a Substitute for an Anti-Coccidial Agent on Growth Performance and Physiological and Immunological Responses in Broiler Chickens Challenged with Eimeria Species.

To overcome the antimicrobial residues in food, benzoic acid (BA) and oregano essential oil (OEO) are used in the broiler chicken industry. Independently, both exerted anticoccidial and antimicrobial actions and improved growth performance in broiler chickens. Their effect may be multiplied when they are used in combination. This present study was carried out to evaluate the efficacy of dietary BA and OEO alone or in combination as a substitute for a commercial coccidiostatic drug on growth performance and physiological and immunological responses in broiler chickens challenged with Eimeria species. A total of 252 unsexed 1-day-old broiler chicks were equally allotted to 36 pens, each pen containing seven chicks. The pens were randomly assigned to six treatments with six pens (replicates) for each treatment (n = 6)-(i) negative control, (ii) positive control, coccidia-challenged and non-treated, (iii) supplemented with salinomycin (an anti-coccidial drug) at 60 mg/kg of feed and coccidia-challenged, (iv) supplemented with BA at 500 mg/kg of feed and coccidia-challenged, (v) supplemented with OEOat 500 mg/kg of feed and coccidia-challenged (OEO), and (vi) supplemented with BA at 500 mg/kg of feed and OEO at 500 mg/kg of feed and coccidia-challenged (B&O). The liver enzymes and thyroxine and creatinine levels were not affected (p > 0.05) both in coccidia-challenged and supplemented chickens. The BA and OEO applied separately or in combination (B&O) significantly (p < 0.05) reduced gut pathogenic bacteria (Salmonella and Escherichia coli) and Eimeria spp., and concurrently enhanced (p > 0.05) the Lactobacillus population with better body weight gain, improved feed utilization, and superior hematological values. It also up-regulated (p > 0.05) the interferon-γ gene expression and down-regulated (p < 0.05) the interleukin-10 and Toll-like receptor-4 gene expression to protect the chickens from inflammatory reactions, which were not demonstrated in salinomycin-treated birds. The B&O supplementation increased (p < 0.05) the immune system by enhancing Eimeria-specific immunoglobulin Y titer and lymphocyte proliferation response. This study suggests that the combined application of OEO and BA can substitute for a commercial anti-coccidial agent (salinomycin) in controlling coccidiosis as well as improving growth performance, gut health, and immune responses in broiler chickens with a means of antimicrobial-resistant free food products.

Identification of lncRNAs regulating seed traits in Brassica juncea and development of a comprehensive seed omics database.

Brassica juncea is a crucial oilseed crop, and its seeds possess high economic value as they are a source of edible oil. In order to understand the role of long non coding RNAs (lncRNAs) in the regulation of seed development, we carried out computational analysis using transcriptome data of developing seeds of two contrasting genotypes of B. juncea, Pusajaikisan (PJK) and Early Heera 2 (EH2). The seeds were sampled at three stages, 15, 30, and 45 days after pollination. We identified 1,539 lncRNAs, of which 809 were differentially expressed. We also carried out extensive characterization and functional analysis of seed lncRNAome. The expression patterns were analysed using k-means clustering, and the targets were analysed using pathway, transcription factor, and GO enrichment, as well as ortholog information. We shortlisted a total of 25 robust lncRNA candidates for seed size, oil content, and seed coat color. We also identified 4 lncRNAs as putative precursors of miRNAs regulating seed development. Moreover, a total of 28 miRNA-lncRNA-mRNA regulatory networks regulating seed traits were identified. We also developed a comprehensive database, (BrassIca juncea database or "BIJ" ( https://bij.cuh.ac.in/ ), which provides seed omics as well as other functional genomics and genetics data in an easily accessible form. These candidate lncRNAs are suitable for including in crop improvement programs through molecular breeding, as well as for future validations through genome editing. Together, the knowledge of these candidate lncRNAs and availability of BIJ database shall leverage the crop improvement efforts in B. juncea.

Multifunctional tri-electrode sensors for the measurement of displacement, force, and infrared irradiation

Abstract Here we described the effect of displacement, force and infrared irradiation on the resistance and impedance of tri-electrode multifunctional sensors. These sensors are based on the gel type composite of carbon nanotubes (CNT), nickel phthalocyanine (NiPc) and edible oil. The channel of this tri-electrodes (field effect transistors) structure is made of CNT-NiPc-oil gel composite using rubbing-in technology. The tri-electrode sensors’ response depends upon the direction of force/displacement and shows an anisotropy. Application of force or displacement from the top causes to decrease resistance and the impedance and vice versa in case of applying force or displacement from the side. The displacement and force sensitivities were up to -273.3 Ω/µm and -46.5 Ω/gf from the top and 480.0 Ω/µm and 3.1 x 102 Ω/gf from the side, respectively, for the sensing ranges 0-150 µm and 0-215 gf. Under the effect of the infrared irradiation from any direction the impedance and the resistance of the sensor reduces. On changing infrared irradiation intensity from 0 to 2500 W/m2 the sensitivities from top and side of the sensor were -37.4 Ωm2/W and -16.5 Ωm2/W, respectively. The investigated sensors may potentially be used as prototypes to develop gel-electronic-based shockproof sensors. The technological achievement in fabricating these devices is the consumption of environmentally friendly materials, particularly edible oil (organic). The edible oil allows to formulate uniform composite gel-films, that may not be comprehended only by ingredients mixing. The fabricated sensors are highly attractive for commercialization.&amp;#xD;