Ofloxacin Complex Research Articles

Spectral, structural, biological and molecular docking studies of a new mixed-valence V(IV)/V(V) ofloxacin complex

In this study, a biologically active mixed-valence V(IV)/V(V) complex [VO(Hofloz)2(H2O)][V4O12]0.5.6H2O (1) (Hofloz = zwitterionic isomer of ofloxacin) was synthesized by the reaction of VOSO4 with ofloxacin (Hofloz) in presence of KOH with 1:1:1 ratio. The complex was characterized through the usage of elemental analysis, FT-IR, molar conductivity and single-crystal X-ray diffraction and thermogravimetric analysis (TGA). The results of single crystal X-ray structure determination revealed an ionic structure consisting of [VO(Hofloz)2(H2O)]2+ and [V4O12]4− units with 1:0.5 stoichiometry. The V+4 ion in the cationic unit has octahedral geometry while each V+5 ion of the eight-member cyclic tetravanadate anion is tetrahedrally coordinated. In regards to the crystal structure of 1, the bidentate ofloxacin ligand is coordinated to the V+4 ion in its zwitterionic form (+HN–···–COO-) through the oxygen atoms of the pyridone and carboxylato groups. The thermodynamic stability of two isomeric forms of Hoflo and 1 along with their charge distribution patterns was studied by DFT and NBO analysis. Also, the ability of the ligand to interact with ten selected biomacromolecules (BRAF kinase, CatB, DNA gyrase, HDAC7, rHA, RNR, TrxR, TS, Top II and B-DNA) was investigated by docking calculations. These studies revealed that this ligand can bind to the proteins and DNA molecule and could be consider as biologically active compound. Finally, the antibacterial activities of the free Hoflo ligand and its vanadium complex have been evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) strains through the utilization of broth microdilution and well diffusion methods. The complex showed antibacterial effect similar to the free Hoflo ligand on the selected bacterial strains and these compounds were more active against Gram-negative bacteria.

Synthesis, Spectroscopic, and Antimicrobial Study of Binary and Ternary Ruthenium(III) Complexes of Ofloxacin Drug and Amino Acids as Secondary Ligands

This article aimed to synthesis, spectroscopic, physicochemical characterizations, and catalytic studies on some ofloxacin (OFL) complexes with ruthenium(III) [Ru(III)] metal ions. The Ru(III)-OFL complex, [Ru(OFL)2(Cl)2]Cl and two mixed-ligand complexes, [Ru(OFL)(AA)(H2O)2]Cl2 derived from OFL as the primary ligand and amino acids [AA; either glycine (Gly) or alanine (Ala)] as the secondary ligands, were synthesized and characterized using microanalytical, spectroscopic, and physical techniques including element composition, molar conductivity, infrared, electronic spectra, thermal, X-ray powder diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX), Scanning electron microscopy (SEM), and Transmittance electron microscopy (TEM) analyses. The element analysis data describe the formation of 1:2 [Ru(III):OFL] and 1:1:1 [Ru(III):OFL:AA] complexes. The octahedral geometry of the complexes was confirmed by their magnetic moment and diffused reflectance. Fourier Transform Infrared spectroscopic (FTIR) measurements suggested that the ligands chelated with Ru(III) ions through the nitrogen atoms of the piperazine ring. In vitro antibacterial efficiency of the OFL compounds was evaluated by paper disc diffusion method. Significant antimicrobial activities were observed for some compounds of the series.

Novel lanthanide pH fluorescent probes based on multiple emissions and its visible-light-sensitized feature

A new type of Eu(III) ofloxacin complex as the fluorescent pH indicator has been presented. Compared to pure ligand, the complex offers more distinguished color changes (green–red–blue) derived from both lanthanide line emissions and the secondary ionization steps of ofloxacin. During the concentration dependence experiments, the photoluminescence studies on the complex showed that the excitation of this pH probe can occur at a very long wavelength which extends to visible range (Ex=427nm). Furthermore, the functional complex was successfully incorporated into soft networks and two novel luminescent hydrogels (rod and film) were fabricated. The soft materials also exhibited specific responses towards the pH variation. Finally, the onion cell-stain experiments were carried out to further confirm the validity of pH dependence and the results support the idea that the material will be suitable for monitoring biological samples in the future.

Interplay of coordinative and supramolecular interactions in directing the crystal structures of copper(II)-ofloxacin complexes under co-ligand intervention

Assemblies of ofloxacin (Hoflo) with Cu(II), in the presence of the organonitrogen ligands, such as 2,2′-bipyridyl (bpy), 4,4′-dimethyl-2,2′-bipyridine (dmbpy), and 8-hydroxyquinoline (hq), under similar conditions, yield a series of three coordination complexes, [Cu(oflo)(bpy)(H2O)](ClO4)(H2O)1.5 (1), [Cu(Hoflo)(dmbpy)(ClO4)](ClO4)(H2O)2 (2), and [Cu(oflo)(hq)](ClO4)(H2O)2 (3). All three complexes have been structurally determined and characterized by physico-chemical and spectroscopic methods. All of the Cu(II) atoms adopt the similar square pyramidal coordination geometries. However, when considering the longer Cu–O contacts in 2, its coordination geometry turns out to be distorted octahedral and a binuclear motif is afforded. The results reveal that, distinct extended network architectures of 1D (for 1) and 2D (for 2 and 3) are further constructed with the aid of weak secondary interactions of H-bonding and aromatic stacking, by introducing various heterocyclic co-ligands. The thermal stabilities of the complexes are described and discussed.

Electrochemical, in-vitro in-vivo study of Co (II)-ofloxacin complex

Co(II)- Ofloxacin complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 30±1 O C and ionic strength of µ= 1.0(KCl). Raper's paper disc method was used for microbial study against various pathogenic bacteria and fungi.Invivo syudy of Swiss mice (25-30gm) were used for antibacterial activity against ofloxacin and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Co(II)-Ofloxacin complex were found to be non-toxic and more potent than pure Ofloxacin.(1)

Determination of Ofloxacin using a Highly Selective Photo Probe Based on the Enhancement of the Luminescence Intensity of Eu3+—Ofloxacin Complex in Pharmaceutical and Serum Samples

A rapid, simple and sensitive spectrofluorimetric method for determination of trace amount of ofloxacin was developed. At pH 5.1 the ofloxacin enhances the luminescence intensity of the Eu(3+) ion in Eu(3+)- ofloxacin complex at λ(ex) = 365 nm. The produced luminescence intensity of Eu(3+)-ofloxacin complex was in proportional to the concentration of ofloxacin. The working range for the determination of ofloxacin was 5.0 × 10(-9)-5.0 × 10(-6) mol L(-1) with lower detection limit (LOD) and quantitative detection limit (QDL) of 3 × 10(-9) and 9 × 10(-9) mol L(-1), respectively. The enhancement mechanism of the luminescence intensity in the Eu(3+)-ofloxacin system has been also explained. The method revealed good selectivity for ofloxacin in the presence of coexisting substances and used successfully for the assay of ofloxacin in pharmaceutical preparations and serum. A comparison with other standard methods was also discussed.

Optical Study of Chitosan-Ofloxacin Complex for Biomedical Applications

Chitosan ofloxacin complex was prepared in isopropyl alcohol under mild conditions. The ionic complexation between chitosan and ofloxacin was confirmed by Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The crystallinity, thermal, surface morphology and optical properties were evaluated by X-ray diffraction (XRD) analysis, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), ultraviolet-visible (UV-Vis) spectroscopy, photoluminescence (PL) spectroscopy and second harmonic generation (SHG) studies, respectively. Absorption of the complex was high (106cm−1) with an optical band gap of 3.80 eV. The chitosan-ofloxacin complex may be considered as a novel optical material from biomedical application point of views. It may find applications as biosensors and environmentally sensitive membranes and artificial membranes.

Flow‐injection with enhanced chemiluminescence detection of ofloxacin in human plasma

A simple, rapid and sensitive method for the determination of ofloxacin in plasma has been developed based on flow-injection analysis with enhanced chemiluminescence (CL) detection. This method employs the CL reaction of cerium(IV) and sulphite sensitized by a Tb(III)-ofloxacin complex. Plasma samples were deproteinized with acetonitrile before analysis. Under optimal conditions, the method allowed the quantitative analysis of ofloxacin in plasma over the range of 22.5-900 ng/mL with a detection limit of 7.5 ng/mL (3sigma). The intra-day and inter-day precision was < 3.0% (n = 11) and < 7.0% (n = 3), respectively. The method was a convenient tool, rapid (20 s/analysis) and well suited for pharmacokinetics studies in which thousands of samples must be analysed daily. It was applied to pharmacokinetics studies of ofloxacin.

Complexes of Ni(II) and Cu(II) with ofloxacin. Crystal structure of a new Cu(II) ofloxacin complex.

Several coordination compounds formed between Ni(II) or Cu(II) with ofloxacin have been synthesised and characterised. According to elemental chemical analysis and FT-IR spectroscopy data, direct reaction of Ni(II) and Cu(II) salts with ofloxacin leads to formation of precipitates for which mass spectrometry demonstrates their polymeric nature. However, crystalline [Cu(oflo)2(H2O)].2H2O is formed if the reaction is carried out in the presence of ammonia. This complex crystallises in the triclinic system, space group P-1 with a=9.2887(12), b=11.2376(14), c=17.874(2) A, alpha=92.12(3), beta=95.39(3), gamma=91.71(3) degrees and Z=2. The local geometry around the Cu(II) ion is a slightly distorted square base pyramid. Electronic spectra, magnetic susceptibility measurements and EPR spectra of the synthesised complexes indicate a tetragonal environment.

Interfacial phenomena involving Fe(III)–ofloxacin complexes at the water|1,2-dichloroethane interface

Abstract When the interface formed between an aqueous solution containing ofloxacin, a fluorquinolone antibiotic complexed with Fe(III), and an organic solution of a supporting electrolyte in 1,2-dichloroethane is potentiodynamically polarized, interesting electroadsorption phenomena are observed. The narrow negative voltammetric peak observed at much lower potentials than those corresponding to the diffusional transfer of a triply charged species, the linear response of I p with v , the difference in the potentials of the positive and negative processes and the shift of this peak towards more negative potentials with sweep rate as coverage is increased, provide clear evidence of an irreversible adsorption–desorption process displaying attractive interaction between the adsorbate molecules at high coverage. These results are reinforced by the appearance of an important charge transfer resistance value in ac measurements.