The dimensional stability of the off-axis optical system affects its optical performance significantly and may even lead to its failure. Here, we report a heterodyne interferometry technology to measure the telescope’s dimensional stability. In this method, the standard plane mirror, which reflects the measurement laser, is a critical structural component. Additionally, it is one of the primary sources of the noise. The study focuses on the impact of the standard plane mirror’s positional misalignment instability on optical path noise. A mathematical model was established to depict the effects of mirror misalignment instability on the optical path, and experiments were conducted to decouple the noise. The results of the experiment show that the standard plane mirror introduces approximately 1nm/Hz1/2 at 10 mHz of optical path noise into the off-axis system, and the dimensional stability of the off-axis system is about 6.23nm/Hz1/2 at 10 mHz.