Ocimum Tenuiflorum Leaf Extract Research Articles

Effects of AgO incorporation with 2D rGO nanocomposite characteristics and its beneficial electrochemical detection of acetaminophen

In this study, the AgO/rGO nanocomposite was facile synthesized via green molecules controllable route using Ocimum tenuiflorum leaf extract. And, we delve into the electrochemical sensing capacity of nanocomposite for the detection of both acetaminophen and 4-nitrophenols. XRD study revealed that the wider (002) rGO diffraction plane integrates with the Ag crystalline characteristic, with a nanocomposite crystallite size of 10.3 nm. Ocimum tenuiflorum biomolecules effectively reduced the metal ions as well as graphene oxide to the expected elemental compound. The incorporation of various leaf biomolecules such as poly-phenolic and alkaloids was bonded with rGO sheet position in the form of C-OH, C–H, N–H, with C = C molecular presence. The efficient interfacial contact between Ag, O and C (rGO) were investigated using XPS, which claimed that the biomolecular group phenol (O–H) with metallic compound as C-O-Ag around 533.9 eV, which has a significant witness for biomolecules incorporations. An identifiable count of elongated spherical-shaped Ag nanoparticles was stacked as well as incorporated between the thin rGO layers with 31.5 nm of particle size was confirmed by HR-TEM analysis. The electrochemical sensing parameters for acetaminophen the proposed sensor exhibits the lowest limit of detection of 0.25 mM for the linear range of concentrations 0.005 to 0.3 mM and 4-aminophenol exhibits the lowest limit of detection of 0.012 mM for the linear range of concentrations 0.001 to 0.3 mM. Overall, this research underscores the potential of AgO/rGO as a robust and efficient platform for achieving enhanced sensitivity in electrochemical sensing applications.

Green synthesis of reduced graphene oxide anchored Fe3O4‐Pd hetero‐nanostructure: An efficient, magnetically separable and reusable catalyst for Beckmann rearrangement of aldoximes to amides

The multifaceted Fe3O4‐Pd@rGO nanocomposite (NC) has been synthesized successfully by a biogenic method using Ocimum tenuiflorum leaf extract. The preparation of graphene oxide (GO) was done by the well‐known modified Hummer method. The as‐synthesized Fe3O4‐Pd@rGO NC was extensively characterized by various analytical techniques, namely, Fourier transform infrared (FTIR), X‐ray diffraction (XRD), field emission scanning electron microscopy‐energy‐dispersive X‐ray spectroscopy (FESEM‐EDX), high‐resolution transmission electron microscopy (HRTEM), inductively coupled plasma‐atomic emission spectrometry (ICP‐AES), X‐ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), vibrating sample magnetometry (VSM) and Brunauer–Emmett–Teller (BET) surface area analysis. The absence of peak corresponding to υ(CO) at 1732 cm−1 in the FTIR spectrum of Fe3O4‐Pd@rGO enumerates the reduction of GO to reduced graphene oxide (rGO) during Fe3O4‐Pd nanoparticle (NP) synthesis with O. tenuiflorum leaf extract. The XRD pattern reveals the existence of rGO, cubic‐phased spinel‐structured Fe3O4 along with the formation of metallic Pd NP without any impurities. The FESEM image designates the spherical and flake‐like morphology of the NC. The crystallite size of the NC was calculated employing the Debye–Scherrer equation and was found to be 3.45 nm corresponding to the (220) plane. The average particle size of Fe3O4‐Pd NPs as estimated from the particle size distribution curve was ascertained as 3.03 nm. The utmost saturation magnetization value of the NC as evaluated by VSM study has appeared at 0.6219 emu g−1 having coercivity value of 335.1 Oe and remanent magnetization value of 0.0767 emu g−1, suggesting the existence of a ferromagnetic material in the synthesized NC. It was a stable and highly efficient catalyst for Beckmann rearrangement of aldoximes using in an environment‐friendly medium. It afforded up to 99% isolated yield of the preferred products under acid‐free condition, avoiding the use of any harsh chemicals that will be beneficial for future industrial applications. Finally, the magnetic component of the NC allowed for easy recovery of the catalyst, thereby eliminating the chance of leaching of metal atoms, and was reusable till six successive cycles without apparent loss of its catalytic performance.

Green Synthesis and Characterization of Nano Silver Doped Nano Hydroxyapatite (nAg-nHAP) using OcimumTenuiflorum Leaf Extract and Sea Shell - A New Approach

Green Synthesis and Characterization of Nano Silver Doped Nano Hydroxyapatite (nAg-nHAP) using OcimumTenuiflorum Leaf Extract and Sea Shell - A New Approach

Structural, Morphological Studies and Photocatalytic Activity of CdZns Nanoparticles Fused with Ocimum Tenuiflorum Leaf Extract

A group 2-6 ternary semiconductor nanoparticle, CdZnS mixed with Ocimum Tenuiflorum leaf extract was prepared using chemical precipitation method at two different concentration of extract (10 ml and 20 ml). Structural, elemental, morphological, optical analyses were done. Particle size of the prepared sample was calculated using XRD pattern, also structure of the sample was confirmed. Elemental analysis was done using EDAX, confirms the elemental composition in the corresponding ratio. SEM study was employed to analyse the morphology of the sample. UV –Visible spectroscopy was done to analyse the absorption of the sample as well as Tauc plot was used to find the band gap energy. Photocatalytic activity of the sample was studied and investigated that the photocatalytic degradation was enhanced by adding the leaf extract to the CdZnS nanoparticle. The present study concentrates on the structural, morphological and optical changes occurred by adding the leaf extract. Also the effect on photocatalytic activity of CdZnS nanoparticles by adding the leaf extract.

Structural, Morphological, Optical, and Antibacterial Activities of Zinc Oxide Nanopowders: A Comparative Study of Green and Soft Chemical Synthesis

Zinc oxide, a well-known inorganic metal oxide in nanoparticle form, has outstanding antibacterial properties. In this work, the authors focus on determining ZnO nanoparticles’ structural, optical, and antibacterial activity. A simple soft chemical route synthesizes C-ZnO nanoparticles chemically, while the green synthesis method is used to prepare G-ZnO nanoparticles. Ocimum tenuiflorum leaf extract was used to prepare G-ZnO nanopowders. These samples are investigated and compared in terms of their structural, morphological, optical, and antibacterial properties. According to XRD investigations, the synthesized ZnO nanopowders possess a hexagonal structure. The particle size of G-ZnO is smaller than that of C-ZnO nanoparticles. The XPS result revealed the binding and interactions between molecules. The FTIR study confirmed the presence of molecules and their vibrations. UV-vis-DRS spectroscopy was used to investigate optical properties such as reflectance and band gap. The grain size of the G-ZnO nanopowders was decreased, and oxygen vacancy was produced. The antibacterial efficiency of plant extracts against two different bacterial strains, S. aureus (Gram-positive) and E.coli (Gram-negative), has been studied and reported.

Green Synthesis of Silver Nanoparticles Using Ocimum tenuiflorum Leaf Extract and its Antimicrobial Activity against Certain Pathogens

Nanoparticles (NPs) are particles less than 100 nm in size. Silver nanoparticles have many important chemical and biological applications. These applications drag the attention of researchers for the production of silver nanoparticles. The objective of the study is to synthesize silver nanoparticles from the green method without using any hazardous chemicals. In the present study, Ocimum tenuiflorum (Tulsi) leaf extract was used to synthesize silver nanoparticles (Ag-NPs) from silver nitrate. Moreover, Tulsi leaf extract was also exploited, both traditionally and commercially, for their antibacterial potential which is essential oil components. The synthesis of Ag-NPs was visually deduced by a change in colour from light yellow to reddish-brown. UV-Visible Spectrometry, X-ray diffraction (XRD), and Transmission Electron Microscopic (TEM) analysis were then used to characterize synthesized nanoparticles. This study also determined the effect of temperature and effect of silver nitrate concentration on nanoparticle synthesis. Synthesized silver nanoparticles showed absorbance ‘peaks’ at 420 nm with UV-visible spectrometry. X-ray diffraction studies showed that the synthesized silver nanoparticles were crystalline. The mean crystallite size was estimated using the Debye-Scherrer equation. However, TEM showed that silver nanoparticles have spherical (40 nm) shape with a narrow size distribution. These synthesized nanoparticles were used to evaluate antimicrobial activity against the most common pathogens of nosocomial infections, MRSA, E. coli, Salmonella typhi, and Klebsiella pneumoniae (ESBL). O. tenuiflorum extract showed strong potential for the synthesis of silver nanoparticles from silver nitrate and the synthesized silver nanoparticles had efficient antimicrobial properties against different pathogens including drug-resistant pathogens in vitro. These synthesized nanoparticles showed maximum ‘zones’ of inhibition against MRSA among these four organisms.

Design of a Non‐Cytotoxic ZnFe2O4‐CeO2/BRGO Direct Z‐Scheme Photocatalyst with Bioreduced Graphene Oxide as Cocatalyst

AbstractHerein,we report the synthesis of a non‐cytotoxic ternary ZnFe2O4‐CeO2/bioreduced graphene oxide (BRZC) nanocomposite via in situ reflux method using a green solvent. The bioreduced graphene oxide (BRGO) used in the nanocomposite was obtained by reducing graphene oxide (GO) in Ocimum tenuiflorum leaf extract. Thourough structural, morphological, and photochemical characterizations revealed that the BRZC heterojunction possesses highly crystalline nanoparticles, enhanced surface reactive sites and suitable band arrangement for photon adsorption. Such a structure was found to bebeneficial in accelerating photocatalytic reaction synergistically by inhibiting charge carrier recombination. The BRZC nanocomposite showcased superior efficiency towards Chlorpyrifos (0.0634 min−1) andRhodamine B (0.0907 min−1) photodegradation. Detailed investigations revealed a most suitable internal electric field‐driven direct Z‐scheme mechanism between ZnFe2O4 and CeO2,where BRGO acts as a cocatalyst. After multiple photochemical and radical trapping experiments, .OH and O2.− were found to be the active species behind the enahnced activity. The ternary heterojunction was also found to be effective for highly toxic real textile effluents with 82 % degradation efficiency. Moreover, Trypan Blue Exclusion (TBE) assay study demonstrated the non‐cytotoxicity nature of the photocatalyst and a significant decrease in cytotoxicity of the above photocatalytically treated pollutants.

Phytogenic synthesis of Co3O4 nanorods and its application in biomolecule sensing and antifungal activity

An efficient, ease, and environmentally friendly method has been developed using Ocimum tenuiflorum leaf extract as reducing agent to prepare Co3O4 nanorods. The synthesized nanorods have been characterized by using various physicochemical techniques such as XRD, FTIR, TG-DTA, TEM, XPS and evaluated the biological sensing activity of Co3O4/GCE as electrocatalyst against glucose and dopamine etc. Electrochemical measurements reveal that Co3O4/GCE exhibited excellent electrocatalytic activity towards glucose with two different linear counter part, i.e., 200 nM – 110 µM (R2 = 0.9996) & 160 µM – 5.91 mM (R2 = 0.9873). The developed glucose sensor reveals the lowest limit of detection level and sensitivity of glucose is about 50 nM and 1301.39 µAmM–1cm−2, respectively in short response time. The Co3O4/GCE sensor also exhibited electrocatalytic activity towards dopamine with wide response range of 500 nM to 407 µM with the correlation coefficient of R2 = 0.9939 and the sensitivity of Co3O4/GCE calculated to 0.24 µAµM-1cm−2 and the sensor exhibits the detection limit to dopamine around 240 nM. The cobalt oxide nanorods show more potent fungal activity towards the Aspergillus niger and Candida albicans as the standard used.

Ocimum tenuiflorum leaf extract as a green mediator for the synthesis of ZnO nanocapsules inactivating bacterial pathogens

ZnO nanoparticles, as fabricated using Ocimum tenuiflorum leaf extract, have been investigated for their photocatalytic and antimicrobial activities. However, the ZnO nanoparticles were synthesized using precursor, Zn(CH3COO)2·2H2O at different concentrations (5, 10 and 50 mmol kg−1) at 70 °C and pH 11.5. The synthesized materials were characterized by employing Fourier transform infrared spectroscopy, UV–Visible, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and transmission electron microscopy techniques. As recorded from UV–Visible studies, there was a drastic decrease in the energy band gap value (Eg) from 3.63 to 3.20 eV with the concentration of precursor, Zn(CH3COO)2·2H2O. The synthesized materials were examined for their photocatalytic activity towards the degradation of methyl orange dye and also for antibacterial action against Bacillus subtilis, Staphylococcus aureus and Escherichia coli pathogenic bacteria.

Biosynthesis, structural, photoluminescence and photocatalytic performance of Mn/Mg dual doped ZnO nanostructures using Ocimum tenuiflorum leaf extract

Here, pure ZnO, Zn0.98Mn0.02O and Zn0.96Mn0.02Mg0.02O nanostructures using aqueous leaf extract of Ocimum tenuiflorum were reported. XRD patterns revealed that no secondary phase was noticed and ZnO hexagonal wurtzite structure was not altered by Mn/Mg simultaneous doping. Mg co-doping with Zn0.98Mn0.02O considerably decreased the crystallite size from 46 to 34 nm due to ionic size difference. The superior absorbance and tuning of energy gap by Mn/Mg substitution is useful for opto-electronic applications. The presence of Mn and Mg in Zn-Mn-Mg-O network was confirmed by IR analysis. The effective charge separation, enhanced optical absorbance in the visible range, increased surface area and the generation of more defect related states were responsible for the noticed highest photocatalytic activity in Mn/Mg doped ZnO.

Biosynthesis of selenium nanoparticles and their effect on changes in urinary nanocrystallites in calcium oxalate stone formation.

Plant bio constituents have the ability to prepare nanoparticles, and usually, plant polyphenols are tested to reduce sodium selenite to selenium nanoparticles (SeNPs). In this work, we showed the biosynthesis of SeNPs using Ocimum tenuiflorum leaf extract. The as obtained SeNPs were in the size range of 15-20nm and spherical in shape. Also, TEM microscopic images represented the aggregation of crystal structures as extracellular deposits. Moreover, scanning electron microscopy was performed to examine the chemical transition of calcium oxalate (CaC2O4) crystal's shape and structure due to the influence of SeNPs. SeNPs inhibited the aggregation and growth of CaC2O4 monohydrate crystals and hence the prepared SeNPs could have important prospects in medical and pharmaceutical applications as a potential inhibitor of CaC2O4 urinary stones.

Biosynthesis of Ag@CuO core–shell nanostructures for non-enzymatic glucose sensing using screen-printed electrode

Ag@CuO core shell nanostructures (ACCS NSs) was successfully synthesized using Ocimum tenuiflorum leaf extract when applied for non-enzymatic glucose sensor. SEM, HR-TEM, XRD, UV–vis, and FTIR were used to estimate the structural, optical and morphological properties of ACCS NSs. Cyclic voltammetry and ampherometry were used to determine the electrochemical and electrocatalytic characteristics of 0.2 wt% ACCS NSs with screen-printed electrodes (SPEs) to sense glucose in 0.1 M NaOH solution. Optimum potential was obtained at + 0.4 V of current response with a linear range of 1 to 9.2 mM. In addition, 0.2 wt% ACCS NSs with SPEs had a low detection limit of 0.006 μM and sensitivity of 3763.44 μA mM−1 cm−2. Modified ACCS/SPE electrodes were highly selective for glucose in the presence of chlorine ions, sugars and non-sugar compounds. It could be inferred from the evidence that the ACCS NSs performs effectively in the aspect of non-enzymatic glucose sensing.

Non-enzymatic biosensing of glucose based on silver nanoparticles synthesized from Ocimum tenuiflorum leaf extract and silver nitrate

An enzyme-free glucose sensor developed based on silver nanoparticles (Ag NPs) via bio-mediated route using Ocimum tenuiflorum leaves extract. The Ag NPs were characterized by X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Particle size analyzer (PSA), Scanning electron microscopy (SEM), Energy-dispersive X-ray (EDAX) spectroscopy, and Transmission electron microscopy (TEM), to study structural, optical and morphological properties. The electrocatalytic activity of Ag NPs towards the oxidation of glucose in the 0.1 M NaOH electrolyte solution was analyzed. The Ag NPs was coated on Glassy carbon electrode (GCE) and reports indicating the outstanding capability such as high sensitivity (895.8 μAmM−1cm−2), linear range (1–8.9 mM), response time (<4s), low detection limit (0.0048 μM, S/N = 3), long-term stability, reproducibility, repeatability, and selectivity of the sensor. Therefore, the bio-synthesized Ag NPs can markedly helpful to fabricate non-enzymatic, sustainable, simple, low cost, and eco-friendly glucose monitoring devices.

Novel synthesis and characterization of pristine Cu nanoparticles for the non-enzymatic glucose biosensor.

Non enzymatic electrochemical glucose sensing was developed based on pristine Cu Nanopartilces (NPs)/Glassy Carbon Electrode (GCE) which can be accomplished by simple green method via ocimum tenuiflorum leaf extract. Then, the affect of leaf extract addition on improving Structural, Optical and electrochemical properties of pristine cu NPs was investigated. The synthesized Cu NPs were characterized with X-ray diffraction (X-ray), Uv-Visible spectroscopy (Uv-Vis), Fourier transformation infrared spectroscopy (FTIR), Particle size distribution (PSA), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDS), Transmission electron microscopy (TEM) for structural optical and morphological studies respectively. The synthesized Cu NPs were coated over glassy carbon electrode (GCE) to study the electrochemical response of glucose by cyclic voltammetry and ampherometer. The results indicates that the modified biosensor shows a remarkable sensitivity (1065.21 μA mM-1 cm-2), rapid response time (<3s), wide linear range (1 to 7.2 mM), low detection limit (0.038 μM at S/N = 3). Therefore, the prepared Cu NPs by the Novel Bio-mediated route were exploited to construct a non-enzymatic glucose biosensor for sustainable clinical field applications.

Characterization Studies of Green Derivitized Silver Nanoparticles Using Ocimum tenuiflorum Leaf Extract and Their Antibacterial Activity

Characterization Studies of Green Derivitized Silver Nanoparticles Using <i>Ocimum</i> <i>tenuiflorum</i> Leaf Extract and Their Antibacterial Activity

Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity

Biosynthesis of nanoparticles is under exploration due to wide biomedical applications and research interest in nanotechnology. We herein reports bioinspired synthesis of silver nanoparticles with the aid of novel, non toxic ecofriendly biological material namely Ocimum tenuiflorum leaf extract. It acts as reducing as well as stabilizing agent. An intense surface plasmon resonance band at ∼450nm in the UV–visible spectrum clearly reveals the formation of silver nanoparticles. The photoluminescence spectrum was recorded to study excitation and emission. TEM and PSD by dynamic light scattering studies showed that size of silver nanoparticles to be in range 25–40nm. Face centered cubic structure of silver nanoparticles are confirmed by SAED pattern. The charge on synthesized silver nanoparticles was determined by zeta potential. The colloidal solution of silver nanoparticles were found to exhibit high antibacterial activity against three different strains of bacteria Escherichia coli (Gram negative), Corney bacterium (gram positive), Bacillus substilus (spore forming).