Ochrobactrum Intermedium Strain Research Articles

Isolation, Characteristics, and Prospects of Using the Ochrobactrum Intermedium Strain in the Degradation of the Cypermethrin Pesticide

Isolation, Characteristics, and Prospects of Using the Ochrobactrum Intermedium Strain in the Degradation of the Cypermethrin Pesticide

Antimicrobial and Antibiofilm Activities of Bacterial Strain P-6B from Segara Anakan Against MRSA 2983

Methicillin-resistant Staphylococcus aureus (MRSA) infection has become a worldwide concern due to the increasing MRSA resistance to antibiotics. This condition encourages the continuous exploration of new anti-MRSA compounds. Thus, this study explored the effect of carbon (starch, glucose, sucrose, lactose) and nitrogen (casein, yeast extract, urea, peptone) sources for the fermentation medium to produce antibacterial and antibiofilm compounds from bacterial strain P-6B against MRSA 2983. The results showed that six-day incubation produced bioactive extracts with the highest antimicrobial activity (11.361 ± 0.860 mm) and the highest activity to inhibit biofilm formation (89.159 ± 0.293% %). In comparison, three-day incubation produced a bioactive extract with the highest activity to degrade biofilm (86.450 ± 3.260%). Glucose was the best carbon source to produce an extract with antibacterial and biofilm inhibition activities. However, starch was the best carbon source to produce an extract with biofilm degradation activity. Urea was the best nitrogen source to produce extracts with antimicrobial and antibiofilm activities. Species identification based on the 16S rRNA gene revealed a 99.78% similarity with Ochrobactrum intermedium strain NBRC 15820 (NR_113812.1). These findings suggested the potency of Ochrobactrum P-6B from Segara Anakan Cilacap as indigenous sources of bioactive compounds with anti-MRSA activities.

The potential of endophytic bacteria to suppress bacterial leaf blight in rice plants

Abstract. Rahma H, Nurbailis, Busniah M, Kristina N, Larasati Y. 2021. The potential of endophytic bacteria to suppress bacterial leaf blight in rice plants. Biodiversitas 23: 775-782. Endophytic bacteria are potential as biocontrol agents against bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae in rice to support sustainable agriculture. This study aimed to select and characterize 22 endophytic bacteria isolated from healthy rice, determine their ability to promote plant growth and suppress bacterial leaf blight disease in rice and also identify potential endophytic bacterial isolates. The study was arranged in a Completely Randomized Design with 24 treatments and three repetitions. The treatments used in the current study consisted of Xanthomonas oryzae infected plants and treated with endophytic bacterial isolates; infected plants without endophytic bacteria treatment (positive control), non-infected plants (negative control). Identification of potential endophytic bacteria was performed based on 16S rRNA sequences. Three out of 22 bacterial isolates, i.e., LmB1, LmA6, and LmB2 were able to suppress bacterial leaf blight disease with severity levels of 35.82%, 23.78%, and 23.78%, respectively. Based on the rice plant growth parameters, three bacterial isolates (LmA6, LmB1, and LmB35) were able to increase the growth of rice plants with an average value of 69.56%, 56.51%, and 47.82%, respectively. Two bacterial isolates, i.e., LmB 1 and LmA6 suppress the development of bacterial leaf blight disease and increase the growth of rice plants. Based on DNA sequence comparisons of DNA fragments amplified by 16S rRNA related marker of the selected bacterial isolates and database, then LmA6, LmB2, LmB1, and LmB35 had similarities with Bacillus cereus MD152 (96.87%), Bacillus thuringiensis ATCC 10792 (98.20%), Ochrobactrum intermedium strain OI1 (97.52%), and Stenotrophomonas maltophilia strain A1w2 (97.92%), respectively. Our study revealed that the indigenous endophytic bacteria from rice plants could be potential biological agents for controlling bacterial leaf blight disease and increasing plant growth.

Sustainable bioelectricity production from Amaranthus viridis and Triticum aestivum mediated plant microbial fuel cells with efficient electrogenic bacteria selections

Application of plant-mediated Microbial fuel cell (PMFC) for bioelectricity production with food crop yield has not yet been appropriately investigated. This study aims to reveal the ability of two essential food plants: Amaranthus viridis (P1) and Triticum aestivum (P2), for sustainable bioenergy production and food harvest for 180 days consecutively. The study showed that the highest power density obtained from P2-PMFC was higher than the P1-PMFC, and the organic-rich nutrient called bioslurry in PMFCs shows twofold intensification in the electrochemical reaction. The main role of bioslurry (brown gold) as a magical organic-rich nutrient increases the plant growth and the activity of electrogenic bacteria in PMFCs. Thus the current density of P1 (114.52 ± 20.05 mA/m2) and P2 (185.23 ± 15.10 mA/m2) without bioslurry was increased with a maximum current density of 185.23 ± 15.10 mA/m2 (P1) and 291.23 ± 7.50 mA/m2 (P2) with bioslurry. The high content screening technique and scanning electron micrograph confirm the biofilm formation on the rhizosphere-anode region of both the plants. Culturable rhizosphere-anode region bacterial species were isolated in P1 and P2 PMFCs, and their electrogenic activity was confirmed using the single-chambered Microbial fuel cells (MFCs) through polarization test. The constructed phylogenetic tree for the 31 isolates concludes the diversity of all bacterial species, of which the phylum Firmicutes dominates in both PMFCs. The individual polarization curve efficiency of the isolates ranges from 7.62 to 194.45 mW/m2, and among the 31 isolates, Hydrogenophaga sp. JBP18, Pseudomonas aeruginosa JBP1, Ralstonia sp. JBP24, Ochrobactrum intermedium strain JBP22 and Bacillus safensis JBP6 were selected as potential electrogenic bacteria. Accordingly, this study might lead to the development of MFC technology for sustainable electricity production using food crop plants and determining more rhizosphere-anode electrogenic bacterial species in the future.

Effects of Various Phosphates on Biosurfactant Fermentation by Ochrobactrum Intermedium XY-1

Phosphates are important components in the fermentation medium for biosurfactant production. In the present study, Ochrobactrum intermedium strain XY-1 was used as the experimental strain, the effects of various phosphates used in the biosurfactants fermentation media was preliminarily investigated on cell growth, and surface tension, and emulsifying activity of fermentation broth. Under the same total phosphorus concentration of 0.035 mol/L, KH2PO4 + Na2HPO4, KH2PO4, and Na2HPO4 was respectively used as the phosphorus source of biosurfactant fermentation at 30 °C and 160 rpm. The results showed that the bacteria grew slowly and the maximum emulsifying activity of fermentation broth reached 52.95% in the fermentation with KH2PO4 + Na2HPO4 as phosphorus source, and larger bacterial growth and 35.9% maximum emulsifying activity exhibited in the fermentation with KH2PO4, smaller bacterial growth and 24.54% maximum emulsifying activity exhibited in the fermentation with Na2HPO4. The phosphate mixture of KH2PO4 and Na2HPO4 benefits the biosurfactant fermentation by limiting the growth of Ochrobactrum intermedium strain XY-1, which might be the earliest report on the phosphorous source of biosurfactant fermentation by Ochrobactrum intermedium.

Nephroprotective roles of potent medicinal plants against acetaminophen (N-acetyl-P-aminophenol) induced kidney damage in mice

Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7. The compound's mineralization and the cleavage of the heterocyclic ring were shown in the experiments with 14C-labeled piracetam. This is also the first report of a pharmaceutical's degradation by the Ochrobactrum genus. This study presents model microorganisms that can be used in further investigation of piracetam's degradation pathways as well as enzymes and genes involved in the process.

Production of antibody-fragments with plasmid-based and genome-integrated T7 E. coli expression systems – evaluation of systems performance in microtiter fed-batch-like cultivations

Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7. The compound's mineralization and the cleavage of the heterocyclic ring were shown in the experiments with 14C-labeled piracetam. This is also the first report of a pharmaceutical's degradation by the Ochrobactrum genus. This study presents model microorganisms that can be used in further investigation of piracetam's degradation pathways as well as enzymes and genes involved in the process.

Stabilization of endangered heritage objects: call a microbial plumber!

Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7. The compound's mineralization and the cleavage of the heterocyclic ring were shown in the experiments with 14C-labeled piracetam. This is also the first report of a pharmaceutical's degradation by the Ochrobactrum genus. This study presents model microorganisms that can be used in further investigation of piracetam's degradation pathways as well as enzymes and genes involved in the process.

Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application

BackgroundAlkaline thermostable lipase and biosurfactant producing bacteria are very interested at detergent applications, not only because of their eco-friendly characterize, but alsoproduction lipase and biosurfactant by using cheap materials. Ochrobactrum intermedium strain MZV101 was isolated as washing powder resistant, alkaline thermostable lipase and biosurfactant producing bacterium in order to use at detergent applications.MethodsO. intermedium strain MZV101 produces was lipase and biosurfactant in the same media with pH 10 and temperature of 60 °C. Washing test and some detergent compatibility character of lipase enzyme and biosurfactant were assayed. The antimicrobial activity evaluated against various bacteria and fungi.ResultsLipase and biosurfactant produced by O. intermedium strain MZV101 exhibited high stability at pH 10–13 and temperature of 70–90 °C, biosurfactant exhibits good stability at pH 9–13 and thermostability in all range. Both lipase and biosurfactant were found to be stable in the presence of different metal ions, detergents and organic solvents. The lipase enzyme extracted using isopropanol with yield of 69.2% and biosurfactant with ethanol emulsification index value of 70.99% and yield of 9.32 (g/l). The single band protein after through from G-50 Sephadex column on SDS-PAGE was calculated to be 99.42 kDa. Biosurfactant O. intermedium strain MZV101 exhibited good antimicrobial activity against Gram-negative bacteria and against various bacterial pathogens. Based upon washing test biosurfactant and lipase O. intermedium strain MZV101considered being strong oil removal.ConclusionThe results of this study indicate that isolated lipase and biosurfactant with strong oil removal, antimicrobial activity and good stability could be useful for detergent applications.Graphical abstract

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications.

The Plackett–Burman design and the Box–Behnken design, statistical methodologies, were employed for the optimization lipase and biosurfactant production by Ochrobactrum intermedium strain MZV101 in an identical broth medium for detergent applications. Environmental factor pH determined to be most mutual significant variables on production. A high concentration of molasses at high temperature and pH has a negative effect on lipase and biosurfactant production by O. intermedium strain MZV101. The chosen mathematical method of medium optimization was sufficient for improving the industrial production of lipase and biosurfactant by bacteria, which were respectively increased 3.46- and 1.89-fold. The duration of maximum production became 24 h shorter, so it was fast and cost-saving. In conclusion, lipase and biosurfactant production by O. intermedium strain MZV101 in an identical culture medium at pH 10.5–11 and 50–60 °C, with 1 g/L of molasses, seemed to be economical, fast, and effective for the enhancement of yield percentage for use in detergent applications.

Isolation of two Ochrobactrum sp. strains capable of degrading the nootropic drug—Piracetam

Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7. The compound's mineralization and the cleavage of the heterocyclic ring were shown in the experiments with 14C-labeled piracetam. This is also the first report of a pharmaceutical's degradation by the Ochrobactrum genus. This study presents model microorganisms that can be used in further investigation of piracetam's degradation pathways as well as enzymes and genes involved in the process.

Prevalence, Host Range, and Comparative Genomic Analysis of Temperate Ochrobactrum Phages

Ochrobactrum and Brucella are closely related bacteria that populate different habitats and differ in their pathogenic properties. Only little is known about mobile genetic elements in these genera which might be important for survival and virulence. Previous studies on Brucella lysogeny indicated that active phages are rare in this genus. To gain insight into the presence and nature of prophages in Ochrobactrum, temperate phages were isolated from various species and characterized in detail. In silico analyses disclosed numerous prophages in published Ochrobactrum genomes. Induction experiments showed that Ochrobactrum prophages can be induced by various stress factors and that some strains released phage particles even under non-induced conditions. Sixty percent of lysates prepared from 125 strains revealed lytic activity. The host range and DNA similarities of 19 phages belonging to the families Myoviridae, Siphoviridae, or Podoviridae were determined suggesting that they are highly diverse. Some phages showed relationship to the temperate Brucella inopinata phage BiPB01. The genomic sequences of the myovirus POA1180 (41,655 bp) and podovirus POI1126 (60,065 bp) were analyzed. Phage POA1180 is very similar to a prophage recently identified in a Brucella strain isolated from an exotic frog. The POA1180 genome contains genes which may confer resistance to chromate and the ability to take up sulfate. Phage POI1126 is related to podoviruses of Sinorhizobium meliloti (PCB5), Erwinia pyrifoliae (Pep14), and Burkholderia cenocepacia (BcepIL02) and almost identical to an unnamed plasmid of the Ochrobactrum intermedium strain LMG 3301. Further experiments revealed that the POI1126 prophage indeed replicates as an extrachromosomal element. The data demonstrate for the first time that active prophages are common in Ochrobactrum and suggest that atypical brucellae also may be a reservoir for temperate phages.

Genome sequencing analysis reveals virulence-related gene content of Ochrobactrum intermedium strain 229E, a urease-positive strain isolated from the human gastric niche.

We report draft genome sequence of Ochrobactrum intermedium strain 229E concurrent with Helicobacter pylori in urease positive gastric biopsy of non-ulcer dyspeptic individual from Southern part of India. Since the role of Ochrobactrum in human gastric environment is poorly understood, comprehensive pathological, microbiological, and genome level understanding are necessary to evaluate its association with H. pylori in the gastric niche. Comparative analysis of O. intermedium 299E strain revealed functional similarities with virulence related gene clusters present in H. pylori genomes, which probably might aid in its ability to persist in the human gastric mucosa. However, H.pylori specific vacuolating cytotoxin (vacA) involved in vacuolization, cytotoxicity, and T-cell inhibition was absent in the O. intermedium 229E genome. Taken together, O. intermedium 229E shared numerous features like secretion system, urease, and flagella with H.pylori genome sequence that might aid concurrence in the gastric niche.

Biopesticide activity of sugarcane associated rhizobacteria: Ochrobactrum intermedium strain NH-5 and Stenotrophomonas maltophilia strain NH-300 against red rot under field conditions.

Colletotrichum falcatum is the major fungal pathogen causing sugarcane red rot. Four antagonistic bacterial strains exhibiting biocontrol activity against this pathogen in greenhouse conditions were characterized for production of different antifungal metabolites and biocontrol determinants to elucidate the mechanism of action involved in their antagonistic activity. The strains were also evaluated under field conditions to assess their biocontrol potential. All the strains produced hydrogen cyanide (HCN), and volatile and diffusible antibiotics. In addition, the Ochrobactrum intermedium strain NH-5 produced siderophores and the broad spectrum antibiotic 2, 4-diacetylphloroglucinol (2,4-DAPG); Pseudomonas sp. NH-203 produced siderophores, and Pseudomonas sp. NH-276 produced protease. Two strains, Ochrobactrum intermedium NH-5 and Stenotrophomonas maltophilia NH-300, exhibited good biocontrol activity, suppressing red rot by 44–52% on two sugarcane varieties, SPF-234 and Co-1148, in field experiments. The strains gave consistent results in three consecutive years and showed potential to be used as biopesticides.

Azo Dye Decolorization under Microaerophilic Conditions by a Bacterial Mixture Isolated from Anthropogenic Dye‐Contaminated Soil

ABSTRACTSoil samples isolated from dye-contaminated sites were exploited for isolation of dye decolorizing microorganisms. A novel bacterial mixture, RkNb1, was selected based on its efficiency, showing maximum and faster decolorization of textile dyes. Seven bacterial strains were isolated and identified from the bacterial mixture as Ochrobactrum intermedium (HM480365), Ochrobactrum intermedium strain M16-10-4 (HM030758), Enterococcus faecalis (HM480367), Arthrobacter crystallopoietes (HM480368), Kocuria flavus (HM480369), Bacillus beijingensis (HM480370), and Citrobacter freundii (HM480371) by 16S rRNA gene sequence analysis. This bacterial mixture showed 98.17% decolorization of Reactive Violet 5 (400 mg L−1) within 8 h. The culture exhibited good decolorization ability at pH 8 and at a temperature of 37°C. Malt extract and peptone was found to enhance the decolorization rate of Reactive Violet 5. Plackett-Burman experimental design was used for elucidation of medium components affecting Reactive Violet 5 decolorization. Dye degradation products obtained during the course of decolorization were analyzed by high-performance thin-layer chromatography (HPTLC), Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR). The potential of this bacterial mixture to decolorize Reactive Violet 5 dye from manufacturing industry effluent is to be carried out using appropriate bioreactors.

Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India

BackgroundOchrobactrum intermedium is an emerging opportunistic pathogen of humans that is closely related to members of the genus Brucella. Earlier, we reported the case of an Indian subject with non-ulcer dyspeptic symptoms whose urease positive gastric biopsy revealed the presence of Helicobacter pylori along with non-Helicobacter like bacteria, eventually cultured and identified as O. intermedium strain M86.ResultsHere, we describe the unclosed draft genome of the strain M86 with a length of 5,188,688 bp and mean G+C content of 57.9%. We have also identified many putative gene clusters that might be responsible for its persistence in the gastric mucosa.Comparative analysis of genomic features of Ochrobactrum intermedium strain M86 and Ochrobactrum intermedium LMG 3301T was also done.ConclusionsThis paper attempts to gain whole-genome based insights into the putative gene determinants of O. intermedium for survival in the highly acidic stomach lumen environment .Identification of genes putatively involved in the various metabolic pathways may lead to a better understanding of the survival of O. intermdedium in acidic condition.

Characterization of an Ochrobactrum intermedium strain STCr-5 manifesting high level Cr(VI) resistance and reduction potential

The investigation was conducted to evaluate the Cr(VI) resistance and reduction by a bacterial strain STCr-5 under different conditions. Strain STCr-5 tolerated very high concentration of Cr(VI) i.e., >10 mg ml −1 in nutrient broth and up to 8 mg ml −1 in M9 minimal medium. Based on 16S rRNA gene sequence homology, the isolate was identified as Ochrobactrum intermedium. It showed up to 90% reduction with different forms of Cr(VI) (CrO 4 2−, Cr 2O 7 2−). O. intermedium STCr-5 exhibited a broad range of Cr(VI) reduction efficiencies at temperatures from 28 to 50 °C and pH levels from 6 to 8. Cr(VI) reduction capability was related to initial cell concentration and Cr(VI) concentration. O. intermedium STCr-5 demonstrated highly efficient Cr(VI) reduction over the Cr(VI) concentration range 100–1000 μg ml −1. Strain STCr-5 reduced 200 μg Cr(VI) ml −1 within 72 h and displayed 50% reduction at the highest Cr(VI) concentration of 1000 μg ml −1 within 96 h. Cr(VI) reduction ability of strain STCr-5 was not influenced by sulphate and nitrate up to 1000 μg ml −1. The presence of other metals (Cu 2+, Co 2+, Mn 2+, Ni 2+, Zn 2+) significantly stimulated Cr(VI) reduction by this isolate. O. intermedium STCr-5, thus, holds great promise for detoxification of Cr(VI) under a wide range of environmental conditions.

Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals

A Cr(VI) resistant bacterial strain SDCr-5, identified as Ochrobactrum intermedium on the basis of 16S rRNA gene sequencing, was tolerant to high concentrations of Cr(VI) up to 15 mg ml −1 in acetate minimal medium. O. intermedium SDCr-5 reduced Cr(VI) under a wide range of concentrations from 100 to 1500 μg ml −1 and reduction was optimum at 37 °C and pH 7. It reduced 200 and 721 μg ml −1 Cr(VI) within 72 and 96 h, respectively. The rate of Cr(VI) reduction increased with concentration from 100 to 1500 μg ml −1. The presence of heavy metal cations such as Cu 2+, Co 2+, Mn 2+ and Ni 2+ stimulated Cr(VI) reduction. Strain SDCr-5 might be useful for Cr(VI) detoxification under a wide range of environmental conditions.