AbstractDuring the survey in greenhouse‐cut flower carnation (Dianthus caryophyllus L.) areas in Izmir, wilted carnation samples were collected from 92 greenhouses. A total of 95 Fusarium spp. isolates were obtained from carnation greenhouses, 80 had been identified as Fusarium oxysporum f.sp. dianthi and published earlier, while the remaining 15 were identified as Fusarium proliferatum (Fp) based on morphological and molecular analysis using TEF1‐α gene sequences in this study. The prevalence of wilting disease of carnation caused by Fp was estimated to be 16.3% of the total area. Pathogenicity tests were conducted by dipping the carnation roots (cv. Turbo) in a spore suspension of monoconidial isolates and transplanting them into pots. Pathogenicity studies confirmed the ability of all Fp to cause symptoms of wilting, leaves and stems shriving. Phylogenetic analysis of sequences from the translation elongation factor 1α (TEF‐1 α) region of the 15 Fp isolates obtained from carnation formed a large clade of isolates originating from carnation and a wide range of other hosts in different geographical locations worldwide. To our knowledge, this is the first report of F. proliferatum causing wilt disease of carnation based on molecular identification in Türkiye.