Substance abuse is a group of diseases or habits that leave serious consequences for human health and life. These include smoking, alcoholism and drug addiction. Substance abuse is also called diseases of conduct. The goal of this research is to assess the prevalence and characteristics of addictions in certain territorial parts of Serbia, based on gender, age, type of settlement, level of education and wealth status. In the implementation of this goal, survey research data were analyzed conducted by the Institute of Public Health of Serbia. The results showed that the addictions are widespread in Serbia. Thus, 10% of school children are constant smokers and 5,4% of them occasionally smoke 2,5 years on average. As for the adult population, 27,7% smoke on daily basis and 5,9% are occasional smokers, so the prevalence of smoking is 33,6%. The average smoking experience is about 19 years. All this is very different in different parts of Serbia, depending on the settlement type, gender, age, education and wealth status. In addition to active smoking on daily basis, there is a high percentage of those who are exposed to cigarette smoke. Only a quarter of the population believes that smoking is harmful, and about a third of the smokers wants to quit smoking. Alcohol consumption is also very widespread - only 57,4% of adults and 63,6% of school children do not drink. There are significant differences by territorial parts of Serbia, settlement type, gender, age, education and wealth status. As well as smoking and alcohol consumption, drug abuse is also widespread in Serbia, although the effects of drugs are known in 35% of cases. The drug is used by about 7% of school children and 17% of adults use pills and 3,5% use marijuana. Of course, there are also significant differences by territorial parts of Serbia, settlement type, gender, age, educational attainment and prosperous state. The general conclusion is that substance abuse in Serbia represents a very significant Social and medical problem.