The study examined the effects of oat β-glucan (OβG), chitosan (CTS), araboxylan (AX), and fructosan (FOS) on wheat dough formation. Adding 0–7 % OβG, AX, and FOS increased SS content, enhancing gluten stability. D-AX and D-FOS showed higher β-sheet structures, higher air retention and gluten network, smaller pores and denser structures, higher elastic and viscosity moduli. Excessive OβG and CTS could reduce the dough stability, and β-turn and β-sheet ratios, respectively. Therefore, B-7AX and B-7FOS exhibited lower hardness indices during storage, leading to a smoother appearance and more orderly gas chamber distribution. The study provides a theoretical foundation for using non-starch polysaccharides in flour-based products.