The Lo Gam structural zone is recognized as a crucial area for the occurrence of lead-zinc deposits in Northeast Vietnam, encompassing the distinguished territories of Cho Dien and Cho Don. Specifically, within the Cho Don area, the Na Bop-Pu Sap area emerges as particularly noteworthy owing to the considerable scale and quality of its lead-zinc ore deposits. In order to comprehensively investigate the geological attributes and lead-zinc mineralization phenomena prevalent in Na Bop-Pu Sap, a suite of analytical techniques including petrographic analyses, mineralogical examinations, scanning electron microscopy (SEM), and ICP-MS were employed. Furthermore, stable isotope investigations and single mineral sphalerite analyses were conducted to assess the origin of metal constituents and the physicochemical conditions conducive to ore genesis. The Na Bop-Pu Sap area is under the influence of the NW-SE fault system, with the ore structure predominantly manifesting as bands and veins aligned parallel to the foliations of hosted rock. This configuration suggests a close association between the formation of lead-zinc ore and the foliation processes occurring within the shear zones. The assemblage of ore minerals within the region primarily comprises galena, sphalerite, pyrite, pyrrhotite, and arsenopyrite. Integration of S and Pb isotopic analyses alongside single mineral sphalerite assessments reveals that the ore-forming solutions emanate from both magmatic activity and sedimentary sources, with lead-zinc ore deposition occurring at temperatures ranging from low to medium. Projections suggest that the Na Bop-Pu Sap mining area may exhibit relative degrees of erosion extending to depths of approximately 600 meters below the existing terrain surface. This comprehensive examination of the ore characteristics in the Na Bop-Pu Sap mine area yields invaluable insights, thereby advancing our understanding of the potential for deep-seated lead-zinc mineral resources within the Northeastern Vietnam.