Microtus thomasi (Rodentia: Arvicolinae), a fossorial vole endemic to the SW Balkans, uses a variety of substrates but its underground behavior remains poorly understood. This study examines the architecture and utilization of M. thomasi burrow systems in NW Peloponnese, Greece. In particular, eight burrow systems were meticulously excavated and studied, with comprehensive measurements taken of key characteristics, including length, depth, soil mounds, and surface openings. Key coordinates were recorded using a differential GPS device for detailed mapping and fractal dimension analysis using the box-counting method was employed to assess burrow system complexity. Soil samples were analyzed for particle size and chemical composition, and vegetation types at each site were identified. We did not find statistically significant correlations between size and complexity of the burrow systems and soil composition, altitude, or specific soil components. On the other hand, we did observe statistically significant differences in tunnel diameter between two burrow systems and in tunnel depth between more. Moreover, our study showed that more than one same-sex individual can occupy a single burrow system and not just an adult male-female pair, that was previously recorded, indicating the need for further study of the social behavior of this vole species. This study provides valuable insights into the underground behavior of M. thomasi by providing information on the features of its burrow systems, thus contributing to our understanding of its biology.
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