Nuttall Oak Research Articles

Resiliency of Nuttall oak but not Shumard oak to winter and spring flood: dormancy alone does not confer flood tolerance

Resiliency of Nuttall oak but not Shumard oak to winter and spring flood: dormancy alone does not confer flood tolerance

Insect wood borers on commercial North American tree species growing in China: review of Chinese peer-review and grey literature.

Invasive insect wood borers are a threat to global forests and tree-related industries as they can damage trees and spread plant pathogens. Reports of damages by wood borers on plants that were planted overseas may facilitate the identification of potential invaders and speed up risk assessment. However, much of this information remains unavailable to the international plant protection community due to language barriers, lack of digitization, or limited circulation of regional literature. Here, we investigated reports of wood borers on 7 important North American commercial tree species planted in China (Carya illinoinensis, Liquidambar styraciflua, Pinus elliottii, Pinus taeda, Quercus texana, Quercus rubra, and Quercus virginiana) in peer-reviewed as well as "grey" (nonpeer-reviewed) Chinese literature. A total of 60 unique wood borer records were found, yielding reports of 4 orders, 39 genera, and 44 species of insect wood borers. Among Coleoptera, longhorned beetles (Cerambycidae) were the most commonly reported colonizers of North American trees in China. Chinese peer-reviewed reports of pests on alien plants are a valuable tool to survey for potential wood-boring invaders of North America, and wherever North American trees are planted and have the potential to encounter Asian invasive insects. Digitization and dissemination of non-English literature are essential for contemporary risk assessment. On the other hand, the nonpeer reviewed "grey" literature, primarily agency reports and student theses, provided only 5% of the records; many incidental observations were unreliable.

Facilitated remediation of heavy metals contaminated land using Quercus spp. with different strategies: Variations in amendments and experiment periods

Phytoremediation using trees combined with soil amendments has gained much attention for its highly cost-effective trait. In natural field conditions, however, the results may not reflect the true performance of amendments based on short-term laboratory studies. In this three-year field trial, various soil amendments such as rice straw biochar, palygorskite, a combined biochar of rice straw biochar and palygorskite, and hydroxyapatite were used to systematically study the potential of the low-accumulator (Quercus fabri Hance) and high-accumulator (Quercus texana Buckley) for cadmium (Cd) and zinc (Zn) to remediate severely contaminated soils. Soil amendments enhanced the dendroremediation capacity of Quercus as the growth period prolonged. In 2021, the rice straw biochar treatment increased Cd and Zn accumulation by 1.76 and 2.09 times in Q. fabri, respectively, compared to the control. Cd and Zn accumulation increased to 1.78 and 2.10 times, respectively, under combined biochar treatment for Q. texana compared to the control. Metals accumulation was mainly enhanced by soil amendments through increasing the growth biomass of Q. fabri and improving the biomass and bioconcentration ability of Q. texana. Overall, soil amendments effectively improved the phytoremediation efficiency of Quercus in the long term, and selecting suitable amendments should be fully considered in phytoremediation.

Forest composition and red oak (Quercus sp.) response to elevation gradients across greentree reservoirs

Elevation gradients within forested wetlands have long been recognized for their role in defining species composition through factors such as hydrology and soil characteristics. Greentree reservoirs (GTRs) are levee-impounded tracts of bottomland hardwood forest flooded throughout the winter months to provide habitat for overwintering waterfowl. Artificial flooding of GTRs alters the forest composition due to flood frequency, depth, and duration in combination with slight changes in topography. To evaluate the effect of elevation gradients, soil properties, and management techniques in the overstory species composition and red oak (Quercus spp.) species abundance, we inventoried 662 plots across 12 independent GTRs in eastern Arkansas. In the lower elevations ranging from 50.98 to 54.99 ​m above sea level, the importance value index (IVI) was highest for nuttall oak (Quercus texana) and overcup oak (Quercus lyrata), whereas IVI shifted to cherrybark oak (Quercus pagoda) in the higher elevations ranging from 54.99 to 58.00 ​m. Alpha diversity did not differ by elevation gradient, soil property, or management technique within GTRs. Beta diversity, using non-metric multi-dimensional scaling (NMDS) analysis, indicated site-specific variability significantly correlated with the environmental predictors, including elevation (R2 = ​0.57), easting (R2 = ​0.47), soil texture (R2 = ​0.21), and pH (R2 = ​0.12). Red oak species-specific mixed-effects modeling of abundance response using Poisson distribution suggested an inverse correlation of nuttall oak and a direct correlation of cherrybark oak abundance with elevation. However, willow oak (Quercus phellos) abundance was not significantly affected by elevation but was by silt loam soil texture and restoration management techniques. These findings will aid management efforts to reduce the dominance of less desirable species that are prominent under specific environmental conditions and promote the dominance of more desirable species. Ultimately GTR sustainability is increasingly important amid the unpredictable impacts of climate change on the preferred red oak species that are economically, ecologically, and environmentally valuable to the sustaining economy of the local community and managing habitats for wildlife.

Growth Response of Red Oaks to Climatic Conditions in the Lower Mississippi Alluvial Valley: Implications for Bottomland Hardwood Restoration with a Changing Climate

Bottomland hardwood forests (BHFs) offer a wide range of ecosystem services that are of high environmental and socioeconomic value. Yet, nearly 70% of BHFs in the southern United States have been lost during the past 100 years primarily due to land use change including agricultural expansion, calling for restoration efforts. We estimated the statistical relationship of the annual radial growth rate of three red oak species with climatic conditions and tree age using the tree ring data collected from a BHF plantation in the Arkansas Delta region. These species were Cherry bark oak (Quercus pagoda), Shumard oak (Quercus shumardii), and Nuttall oak (Quercus texana). The destructive sampling method was employed to obtain tree growth data and the cross-dating method was used for tree age determination. A log-linear regression model was estimated to uncover the statistical relationship between annual tree ring growth rate and climatic conditions. We identified the most critical time windows of climate variables that affect the growth of these trees. We found that the average temperature in October of the previous year and the minimum temperature between December of the previous year and January of the current year were positively associated with the radial growth rate in the current year although the maximum temperature from January to August and total precipitation from April to July of the current year were negatively correlated with the growth rate. Compared to Cherry bark and Shumard oaks, Nuttall oak was less sensitive to a rise in the minimum temperature between December and January. The projected climate change is likely to create slightly more favorable overall climatic conditions for these oak species in the region. Our findings suggest that these three red oak species are well suited for the study region for restoring BHFs, especially with a changing climate.

Evaluation of dendroremediation potential of ten Quercus spp. for heavy metals contaminated soil: A three-year field trial

Woody plants have gained considerable attention for remediating soils contaminated with heavy metals because of their cost-efficient and ecologically friendly nature. However, most studies on potential phytoremediation evaluation are limited to short-term experiments in greenhouse or field, meaning that differences may exist between laboratory results and application in natural environment. In this study, ten Quercus spp. were tested in a consecutive 3-year field trial (2018–2020) to assess their dendroremediation abilities for Cd and Zn contaminated soil. The results revealed that nine Quercus spp. demonstrated good survival ability without any stress, except for Quercus velutina Lam., in the 3-year growth period. In 2020, Quercus texana Buckley and Quercus fabri Hance plants produced the greatest biomass (2100 and 1880 g plant−1) among the nine Quercus spp. Quercus texana had the highest total Cd accumulation (39.3 mg plant−1) in 2020, which was 8.5 times higher than that in 2018, followed by Quercus pagoda Raf. (8.85 mg plant−1) and Q. fabri (8.07 mg plant−1) plants, respectively, whereas Cd accumulation increased by 7.4 times for Q. pagoda and 22 times for Q. fabri compared to 2018. The results from 2020 indicated that Q. fabri had the highest Zn accumulation (205 mg plant−1), followed by Quercus nigra L. (149 mg plant−1) and Q. texana (140 mg plant−1), respectively, and these values increased 14, 6.4, and 6.2 times in comparison to 2018. The comprehensive bioaccumulation index (CBAI) was proposed to evaluate the dendroremediation potential of Quercus spp., suggesting that Q. texana and Q. fabri had the most outstanding potential for remediation of Cd and Zn polluted soil, with the values of 0.82 and 0.60, respectively. In summary, Q. texana and Q. fabri are ideal for remediating Cd/Zn-contaminated soil, and long-term field trials and the CBAI method are helpful for comprehensively evaluating the remediation capacity of trees.

Eleven-Year Effects of Mechanical Site Preparation on Oaks Planted on Former Agricultural Fields

Mechanical site preparation is often prescribed as a tool for correcting soil condition problems encountered when planting former agricultural fields. While the impact of mechanical site preparation on early growth and survival of young oak seedlings is well-known, there is a shortage of information regarding the longer-term effects of these treatments. Four hundred and eighty, 1-0 bare-root seedlings each of Nuttall oak (Quercus texana Buckley), Shumard oak (Quercus shumardii Buckley), and swamp chestnut oak (Quercus michauxii Nutt.) seedlings were planted in February 2008 on a retired agricultural site in northwest Mississippi. Four site preparation treatments were utilized, with 160 seedlings of each species per species/mechanical treatment combination, totaling 1440 seedlings for the study. Mechanical site preparation included control, subsoiling, bedding, and combination plowing treatments applied on 3.1 m row centers. Eleven-year diameter (DBH), total height growth (HT), height-to-live crown (HTLC), and survival data were recorded in early 2019. Treatment effects were analyzed, and significant differences were not detected for tree survival. However, differences were observed in the DBH, HT, and HTLC averages of swamp chestnut oak and the DBH of Shumard oak.

Stem profile of red oaks in a bottomland hardwood restoration plantation forest in the Arkansas Delta (USA)

Bottomland hardwoods are among the most diverse and productive forest ecosystems in the southeastern United States and are critically important for the provision of timber and non-timber ecosystem services. Red oaks, the dominant species in this group of forests, are of high ecological and economic value. Stem profile models are essential for accurately estimating the merchantable volume of oak trees, which is also closely indicative of total tree biomass and other ecosystem services given their allometric relationships. This study aims to develop and compare stem profiles among three red oak species in an 18-year old plantation forest using destructive sampling. Sixty trees randomly selected from an oak restoration plantation in the Arkansas Delta were felled for measuring the diameter-outside-bark (DOB) and diameter-inside-bark (DIB) at different stem heights. These sample composed of twenty trees from each of three species: cherry bark oak (CBO - Quercus pagoda Raf), Nuttall oak (NUT - Quercus texana Buckley), and Shumard oak (SHU - Quercus shumardii Buckl). Multiple models, including the segmented-profile model, form-class profile model, and second-and third-order polynomial models were fitted and compared. Results demonstrate that the form-class profile model was the best fitted for CBO and NUT, whereas the third-order polynomial model was the best for SHU. CBO tends to grow taller and has a higher wood density than NUT and SHU. These findings will inform restoration and management decisions of bottomland hardwood forests, especially red oaks in the region.

Estimating Urban Tree Metrics Using Terrestrial LiDAR Scanning

Trees grown in an urban environment are typically from a selected list of suitable species due to their appearance and other factors. A popular oak species in recent decades has been the Nuttall oak (Quercus texana). A total of seven Nuttall oaks were scanned using a terrestrial LiDAR scanner and modeled for comparison to manual measurements. These trees were then destructively sampled in place to measure their above-ground biomass. The biomass data were compiled and statistically compared against digital models of each tree that were created from the LiDAR scans. This resulted in a Pearson coefficient of .977 and linear regression R2 value of .99 for the LiDAR derived measurements predictive ability in comparison to the manually derived measurements. This indicates an ability of this ground based LiDAR model to predict both the linear dimensions and volumetrics of the standing specimens without the need for such labor intensive and expensive sampling given the sensitivity and value of urban forests.

Floodplain forest tree seedling response to variation in flood timing and duration

The regeneration process is a sensitive period within life cycles of floodplain tree species and can strongly influence forest community composition. Yet, fundamental information remains limited on the relationship between regeneration processes and the flood disturbances that, together, construct floodplain forest landscapes. In a controlled greenhouse experiment we tested the effects of complete submergence on six temperate floodplain forest species to understand how flood timing and duration influence seedling survival. Groups of overcup oak (Quercus lyrata), Nuttall oak (Quercus texana), willow oak (Quercus phellos), sugarberry (Celtis laevigata), green ash (Fraxinus pennsylvanica), and American elm (Ulmus americana) seedlings were submerged for either 5, 15, 25, or 0 days (control) at the ages of 3-weeks, 6-weeks, and 9-weeks post-emergence. All species demonstrated a higher sensitivity to flooding at age 3-weeks compared to 6- and 9- weeks, indicating substantial changes in seedling resilience within the first months following emergence. Additionally, the heavier-seeded Q. lyrata, Q. texana, and Q. phellos were less or equally vulnerable to flooding compared to the lighter-seeded C. laevigata, F. pennsylvanica, and U. americana across all age groups, especially at 3-weeks post-emergence. The results of this study have implications for understanding woody species regeneration ecology and changes in floodplain forest composition, particularly in the context of hydrologic modifications.

Herbivory and flooding impacts on planted bottomland hardwood seedlings

Abstract Bottomland hardwoods forests (BLHW) are increasingly subject to active management for water quality improvement, streambank stabilization, to mitigate for urban expansion and to improve habitat for wildlife. Since the majority of remaining BLHW are degraded, restoration attempts are becoming commonplace within the Western Gulf Coastal Plain (WGCP) of the US. However, restoration success in terms of obtaining high stem densities of desirable species has been mixed, with managers observing survival rates <15 per cent for planted oak and hickory seedlings in some situations due to a variety of limitations. Over two growing seasons, we investigated the effectiveness of portable electric fences, individual tree shelters and 2.4-m woven wire high fences for mitigating herbivory by white-tailed deer (Odocoileus virginianus) and feral swine (Sus scrofa). Treatments fell within degraded forested areas and abandoned agricultural fields where we tested four species of interest: Nuttall oak (Quercus texana Buckley), Shumard oak (Q. shumardii Buckley), bur oak (Q. macrocarpa Michx.) and pecan (Carya illinoinensis K. Koch). Where herbivory occurred, mitigation techniques produced a higher survival rate ($\bar{\rm{X}} $ = 17.6 per cent) than unprotected areas ($\bar{\rm{X}} $ = 9.1 per cent). In areas of high white-tailed deer density, prominent browsing was evident, resulting in two-growing-season height growth of seedlings being less in non-fenced ($\bar{\rm{X}} $ = 2.33 cm) and electric ($\bar{\rm{X}} $ = 4.33 cm) fenced plots compared with high fences ($\bar{\rm{X}} $ = 13.02 cm) and individual tree shelters ($\bar{\rm{X}} $ = 24.23 cm). Additionally, we observed a negative relationship between survival and the number of days seedlings that were inundated with flood waters during the growing season. Matching species of interest to the site conditions, specifically the local hydrologic regimes, should carry a high priority in planning a restoration project within BLHW in the WGCP.

Influence of Soil Saturation on Survival and Growth of Bottomland Oaks Six and Fourteen Years after Planting

Bottomland oak restoration projects have been marginally successful because professionals often recommend conventional tree-planting procedures that prove problematic in hydric soils. A reliable method of matching oak species to bottomland sites is needed prior to planting. To address the issue, we planted 8 different species of oak seedlings into previously farmed hydric soil in West Tennessee in 2004. We took measurements after 6 and 14 growing seasons to ascertain survival and growth trends based on soil mottling and the depth to gleyed matrix. The later measurements mostly supported early findings that Quercus lyrata (Overcup Oak), Q. texana (Nuttall Oak), Q. palustris (Pin Oak), and Q. phellos (Willow Oak) are most tolerant of poorly drained soil and that as soil drainage improves, species diversity may expand.

Tree-level Growth of Four Oak Species in a Bottomland Hardwood Plantation: Implications for Silvicultural Treatments

Abstract A 41-year-old bottomland hardwood plantation experiment located in southeastern Arkansas was revisited in 2017 to evaluate basal area increment (BAI) of four oak species [cherrybark oak (Quercus pagoda Raf.), swamp chestnut oak (Q. michauxii Nutt.), Nuttall oak (Q. texana Buckl.), and water oak (Q. nigra L.)] planted in monoculture in relation with species, spacing, thinning, and neighborhood competition. Species had a significant effect on BAI (P = 0.04), with cherrybark oak and Nuttall oak demonstrating the greatest (21.5 ± 2.5 cm2 year−1) and lowest (12.7 ± 2.9 cm2 year−1) growth rates, respectively, whereas BAI declined significantly with increasing competition (P = 0.03). However, rates of growth decline with competition did not differ between species (P > 0.05 for competition × species term), and thinning and spacing treatments had no effect on BAI (P > 0.05). We recommend that repetitive thinning, rather than a single thinning, would be appropriate to maintain a positive impact on oak growth. Managing stand density would not only provide opportunities for improving timber production, but also would enhance ecological benefits (e.g., wildlife habitat) through diversification of structure within monoculture plantations.

Co-planting of Quercus nuttallii, Quercus pagoda with Solanum nigrum enhanced their phytoremediation potential to multi-metal contaminated soil

To screen the efficient tree-herb co-planting patterns to remediate the heavy metal polluted soil, a greenhouse experiment was conducted for 150 days to examine the plant growth and metals accumulation across three co-planting patterns, including Solanum nigrum (S) co-planted with Quercus nuttallii (NS) or Quecrus pagoda (PS), and those three species are co-planted together (NPS). Results showed that the NPS pattern slightly decreased the tree biomass, while NS and PS treatments improved the plant growth (1.51–10.68%). It is worth noting that the NS treatment significantly (p < 0.05) increased photosynthetic pigment content (82.61–113.93%), net CO2 assimilation (21.44%), and the uptake of Cd (44.58%) in Q. nuttallii; the PS treatment significantly (p < 0.05) increased the net CO2 assimilation (8.61%) and the uptake of Cd (42.23%), Zn (31.18%) in Q. pagoda; and the uptake of Cd and Zn in the NPS co-planting treatment were only slightly increased. For S. nigrum, the photosynthetic pigment content was elevated and the metal accumulation in itself also maintained the relative stable in all the co-planting treatments. Thus, co-planting of Quercus with S. nigrum was a promising way to remediate heavily polluted soil by heavy metals. Novelty statement: Co-planting with multiple plant species, as a novel strategy, has great value for the remediation of heavy metal contaminated soil. The paper aimed to explore the suitable co-planting pattern of Quercus, arbor trees which showed phytoremediation potential, co-planted with Cd hyperaccumulator, Solanum nigrum. The result suggested the co-planting with S. nigrum enhanced the plant growth, photosynthesis, and metals extraction of Q. nuttallii and Q. pagoda. Co-planting also improved ecological adaptation of S. nigrum via elevating pigment content. Thus, co-planting of Quercus with S. nigrum was a promising way to remediate polluted soil.

Autumn flooding disrupts seasonal nitrogen storage and impacts spring growth in Quercus texana seedlings

Autumn flooding reduced nitrogen uptake and fine root mass. Leaf nitrogen resorption increased, but overall autumn nitrogen accumulation was negligible in flooded seedlings. Subsequently, spring survival and growth were decreased. Since nitrogen (N) is often limiting in terrestrial ecosystems, N is conserved in trees by resorption before leaf senescence. Bottomland forests are prone to flooding, which could reduce N uptake, and may decrease phloem transport, which is essential for N resorption. Therefore, we hypothesized that autumn flooding may diminish both N uptake and N resorption in trees, and this would reduce spring growth. Two-year-old Quercus texana seedlings either had no flood or had their complete root system flooded during dormancy induction, the period in autumn when trees prepare metabolically and physiologically for winter dormancy. We measured seedling growth and nutrient contents before flooding and after leaf fall and determined impacts of autumn flooding on growth during the subsequent spring. Autumn flooding resulted in a small increase in N resorption from leaves. In non-flooded seedlings, much more N accumulated in stems and roots than the amount resorbed from leaves, suggesting that there was substantial N uptake during the autumn dormancy induction period. However, flooding severely reduced accumulation of N in roots and stems during autumn, probably by directly reducing uptake and by increasing fine root mortality. Winter survival was reduced 50% by autumn flooding. Autumn-flooded seedlings that survived winter had greater new root growth in spring than non-flooded seedlings, but substantially decreased stem diameter growth. Our results indicate that Q. texana seedlings which are flooded during dormancy induction may be less competitive the following spring due to fine root mortality and reduced nutrient storage, which negatively impact spring growth.

The Influence of Dormancy Break Requirements on Germination and Viability Responses to Winter Submergence in Acorns of Three Bottomland Red Oak (Sect. Lobatae) Species

Abstract The objective of this study was to examine the relation of dormancy break requirements to winter submergence effects on germination and viability in cherrybark, willow, and Nuttall oak acorns. Acorns were submerged in a greentree reservoir and received 0, 21, 42, 63, or 84 days of winter submergence, followed by 8 weeks of incubation in four temperature regimes (15/6°, 20/10°, 25/15°, and 30/20° C). Winter submergence substituted for cold stratification requirements of cherrybark oak acorns, and dormancy break was achieved with 63 days of submergence. Although winter submergence exerted a positive effect on germination in Nuttall and willow oak acorns, dormancy break was not achieved in acorns of either species. Germination percentages were highest in the 30/20° C incubation temperature—cherrybark oak acorns (75–83 percent), followed by Nuttall oak (33–55 percent) and willow oak (14–52 percent). Among treatment combinations, all willow oak nongerminants were viable. Viability loss in cherrybark and Nuttall oak acorns was greatest in the 63- and 84-day treatments. The dynamics of acorn germination in response to winter hydrologic regime is influenced by submergence duration that may, or may not, satisfy dormancy break requirements in acorns of these red oak species.

Effect of Wind on the Relation of Leaf N, P Stoichiometry with Leaf Morphology in Quercus Species

Leaf nitrogen (N) and phosphorus (P) stoichiometry correlates closely to leaf morphology, which is strongly impacted by wind at multiple scales. However, it is not clear how leaf N, P stoichiometry and its relationship to leaf morphology changes with wind load. We determined the leaf N and P concentrations and leaf morphology—including specific leaf area (SLA) and leaf dissection index (LDI)—for eight Quercus species under a simulated wind load for seven months. Leaf N and P concentrations increased significantly under these conditions for Quercus acutissima, Quercus rubra, Quercus texana, and Quercus palustris—which have elliptic leaves—due to their higher N, P requirements and a resultant leaf biomass decrease, which is a tolerance strategy for Quercus species under a wind load. Leaf N:P was relatively stable under wind for all species, which supports stoichiometric homeostasis. Leaf N concentrations showed a positive correlation to SLA, leaf N and P concentrations showed positive correlations to LDI under each wind treatment, and the slope of correlations was not affected by wind, which indicates synchronous variations between leaf stoichiometry and leaf morphology under wind. However, the intercept of correlations was affected by wind, and leaf N and P use efficiency decreased under the wind load, which suggests that the Quercus species changes from “fast investment-return” in the control to “slow investment-return” under windy conditions. These results will be valuable to understanding functional strategies for plants under varying wind loads, especially synchronous variations in leaf traits along a wind gradient.

Rehabilitating Degraded Hardwood Stands on a Bottomland Terrace Site with Overstory Removal and Oak Enrichment Planting: 14-Year Results

Exploitative harvesting can lower stand quality in the short term and diminish forest productivity over the long term. In 2003, a rehabilitation experiment was installed in a southern hardwood stand on a bottomland terrace site, degraded by periodic exploitative cutting, to test the effectiveness of overstory removal and oak enrichment planting for improving stand quality and composition. Overstory removal treatments included clearcutting, stand improvement partial cutting, and an uncut control. Overstory treatment units were either planted with Nuttall oak (Quercus texana) seedlings or not planted. We revisited this study in 2017 to assess the outcome of oak enrichment planting across levels of overstory removal. Results in year 14 indicated poor survival of planted oaks under all overstory removal treatments (14% - 24%) and minimal height growth (0.3 m in 13 years) in areas treated with partial cutting and in uncut areas. Growth performance of planted oaks was significantly enhanced by clearcutting (p < 0.01). However, the overwhelming response of natural oak regeneration initiated by clearcutting trumped the contribution from enrichment planting on this low quality bottomland terrace site. Enrichment planting of Nuttall oak did not increase oak regeneration success in our study. The ineffectiveness of enrichment planting in this study was likely related to the shady understory of partially cut stands and intense competition in clearcuts. Additional treatments, such as pre-planting site preparation and post-planting release may be necessary for enhancing and maintaining competitiveness of planted red oak seedlings on similar sites.

Tissue Culture Using Mature Material for the Conservation of Oaks

Tissue culture using mature-phase plant material is a useful tool for species conservation, but can be a challenge with oak (Quercus) species, often resulting in low growth and survival. Two different tissue culture media were compared and used to determine whether there was a survival, growth, or contamination response pattern in species representing three North American oak taxonomic sections: red oaks (section Lobatae), white oaks (section Quercus), and golden oaks (section Protobalanus). Mature phase cuttings were harvested in springtime from 12 oak species: arkansas oak (Q. arkansana), canby oak (Q. canbyi), slender oak (Q. graciliformis), nuttall oak (Q. texana), boynton sand post oak (Q. boyntonii), california scrub oak (Q. dumosa), engelmann oak (Q. engelmannii), gambel oak (Q. gambelii), canyon live oak (Q. chrysolepis), palmer oak (Q. palmeri), island oak (Q. tomentella), and huckleberry oak (Q. vacciniifolia). Excised shoot-tip explants were placed onto either Lloyd and McCown woody plant (WP) medium or Gresshoff and Doy (GD) medium. More growth responses and longer survival times were seen on explants grown on the WP medium than GD medium. Explants originating from species native to xeric habitats or those with smooth, glabrous young leaves had significantly higher contamination rates. Although no significant differences were found when grouped by taxonomic section, survival, growth, and contamination varied significantly by species. These findings contribute to the process of establishing tissue culture methods using mature oak material, particularly in relation to medium selection and sterilization protocols, which is critical to the conservation of this iconic group of species.

Photo-physiological and photo-biochemical characteristics of several herbaceous and woody species based on FvCB model

To explore the photosynthetic capacity and the leaf photosynthetic apparatus for plants with different life forms, CO2 response curves of 7 woody species and 4 herbaceous species were fitted by the modified rectangular hyperbolic model and the FvCB model, and the photosynthetic parameters, including maximum net photosynthetic rate (Pn max), maximal Rubisco carboxylation rate (Vc max), maximal electron transport rate (Jmax), day respiration (Rd), and mesophyll resistance to CO2 transport (rm), were compared among different woody species, among different herbaceous species, and between woody and herbaceous life-forms, respectively. The results showed Pn max of seven woody species descended in the order of Sapium sebiferum and Boehmeria nivea ＞ Machilus pingii and Pittosporum tobira ＞ Cyclobalanopsis glauca, Castanopsis sclerophylla, and Quercus nuttallii. Vc max of S. sebiferum, B. nivea, M. pingii, and P. tobira was significantly higher than that of C. glauca and C. sclerophylla. Jmax of woody species was in descending order as S. sebiferum ＞ B. nivea and P. tobira ＞ Q. nuttallii, C. sclerophylla, and C. glauca. rm of M. pingii and C. sclerophylla was higher than that of S. sebiferum, P. tobira and B. nivea. Pn max of Phytolacca acinosa was significantly higher than that of Ageratum conyzoides and Achyranthes aspera. There was no significant difference in Vc max among 4 herbaceous species. Jmax of P. acinosa was higher than that of A. conyzoides. rm of S. nigrum and A. aspera was higher than that of A. conyzoides. Rd of P. acinosa was higher than that of A. conyzoides and A. aspera. The photosynthetic parameters (Pn max, Vc max, Jmax and rm) of woody species were significantly higher than those of herbaceous species, but no significant difference was found in Rd between woody and herbaceous species. In conclusion, the difference in photosynthetic capacity among different species and between the two plant life-forms resulted from the difference in Rubisco carboxylation capacity, electron transport capacity, and mesophyll resistance among these species.