Introduction In women of reproductive age, polycystic ovarian syndrome (PCOS) is a prevalent endocrine illness that has detrimental effects on metabolism, reproduction, the endocrine system, and psychological health. The quality of life for women with PCOS is significantly impacted by its symptoms related to excess androgen and atypical menstruation. Objective There is a scarcity of data on female nurses' knowledge concerning PCOS and health-related practices. This study aimed to determine the prevalence, knowledge, and practices of PCOS among female nurses in Lebanon. In addition, we assessed whether these nurses have menstrual irregularities, obesity, hirsutism, extreme acne problems, and whether they are aware of the syndrome or not. Methods Using a self-administered questionnaire in Arabic and English languages, a descriptive cross-sectional study was carried out among Lebanese female nurses. Demographic characteristics were reported using descriptive statistics. The differentiating, associating, and correlating characteristics of the variables were reported using inferential statistics. Results The vast majority of respondents (91.5%) had good knowledge, and (39.6%) had good health-related practices. Nearly half (47.2%) were suspected to develop PCOS, and 8.5% were diagnosed based on signs and symptoms. According to the study's findings, nurses were unaware of the condition even though many exhibit its symptoms. The study also reported that 31.1% of participants were overweight, and eight (7.5%) were obese. Conclusion Despite having knowledge of the PCOS risk factors, females had considerably less practice in related fields of health. Female nurses with suspected or confirmed PCOS should seek immediate medical help since early diagnosis or treatment for PCOS is useful in enhancing their quality of life.