Numerical Dosimetry Research Articles

Enhancing cell motility via non-contact capacitively coupled electrostatic field.

Cellular motility is essential for making and maintaining multicellular organisms throughout their lifespan. Migrating cells can move either individually or collectively by a crawling movement that links the cytoskeletal activity to the adhesion surface. In vitro stimulation by electric fields can be achieved by direct, capacitive or inductive coupled setups. We tested the effects of electrical stimulation provided by capacitive coupling on glioma cells, using a capacitive-coupled system powered by a potential difference of 35V between two electrodes placed outside the culture dish. Numerical dosimetry identified two different fields: (i) in the order of 103 V/m at the level of the dielectric substrates, with almost uniform distribution; (ii) in the order of 10-1 V/m at the level of the culture medium, with spatial and material-dependent distribution. The scratch assay and the tracking of single-cell movement showed a boosted motility when crawling occurs on polystyrene surfaces, demonstrating the feasibility of this peculiar exposure system to generate forces capable of influencing cell behavior.

Stimulus effects of extremely low-frequency electric field exposure on calcium oscillations in a human cortical spheroid.

High-intensity, low-frequency (1 Hz to 100 kHz) electric and magnetic fields (EF and MF) cause electrical excitation of the nervous system via an induced EF (iEF) in living tissue. However, the biological properties and thresholds of stimulus effects on synchronized activity in a three-dimensional (3D) neuronal network remain uncertain. In this study, we evaluated changes in neuronal network activity during extremely low-frequency EF (ELF-EF) exposure by measuring intracellular calcium ([Ca2+]i) oscillations, which reflect neuronal network activity. For ELF-EF exposure experiments, we used a human cortical spheroid (hCS), a 3D-cultured neuronal network generated from human induced pluripotent stem cell (hiPSC)-derived cortical neurons. A 50 Hz sinusoidal ELF-EF exposure modulated [Ca2+]i oscillations with dependencies on exposure intensity and duration. Based on the experimental setup and results, the iEF distribution inside the hCS was estimated using high-resolution numerical dosimetry. The numerical estimation revealed threshold values ranging between 255-510 V/m (peak) and 131-261 V/m (average). This indicates that thresholds of neuronal excitation in the hCS were equivalent to those of a thin nerve fiber.

Monte Carlo Code and Its Role in Numerical Dosimetry

Monte Carlo Code and Its Role in Numerical Dosimetry

Comparison of Transcranial Magnetic Stimulation Dosimetry between Structured and Unstructured Grids Using Different Solvers.

In recent years, the interest in transcranial magnetic stimulation (TMS) has surged, necessitating deeper understanding, development, and use of low-frequency (LF) numerical dosimetry for TMS studies. While various ad hoc dosimetric models exist, commercial software tools like SimNIBS v4.0 and Sim4Life v7.2.4 are preferred for their user-friendliness and versatility. SimNIBS utilizes unstructured tetrahedral mesh models, while Sim4Life employs voxel-based models on a structured grid, both evaluating induced electric fields using the finite element method (FEM) with different numerical solvers. Past studies primarily focused on uniform exposures and voxelized models, lacking realism. Our study compares these LF solvers across simplified and realistic anatomical models to assess their accuracy in evaluating induced electric fields. We examined three scenarios: a single-shell sphere, a sphere with an orthogonal slab, and a MRI-derived head model. The comparison revealed small discrepancies in induced electric fields, mainly in regions of low field intensity. Overall, the differences were contained (below 2% for spherical models and below 12% for the head model), showcasing the potential of computational tools in advancing exposure assessment required for TMS protocols in different bio-medical applications.

Electric field bridging-effect in electrified microfibrils' scaffolds.

Introduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils' scaffolds are capable of supporting cells, promoting their survival and guiding their differentiation in neural lineage to repair the lesion. Despite its biocompatible nature, PLA is an electrically insulating material and thus it could be detrimental for increasingly common scaffolds' electric functionalization, aimed at accelerating the cellular processes. In this context, the European RISEUP project aims to combine high intense microseconds pulses and DC stimulation with neurogenesis, supported by a PLA microfibrils' scaffold. Methods: In this paper a numerical study on the effect of microfibrils' scaffolds on the E-field distribution, in planar interdigitated electrodes, is presented. Realistic microfibrils' 3D CAD models have been built to carry out a numerical dosimetry study, through Comsol Multiphysics software. Results: Under a voltage of 10V, microfibrils redistribute the E-field values focalizing the field streamlines in the spaces between the fibers, allowing the field to pass and reach maximum values up to 100kV/m and values comparable with the bare electrodes' device (without fibers). Discussion: Globally the median E-field inside the scaffolded electrodes is the 90% of the nominal field, allowing an adequate cells' exposure.

Exposure computational models with voxel phantoms coupled to EGSnrc Monte Carlo code

In computational dosimetry of ionizing radiation, the energy deposited in radiosensitive organs and tissues is evaluated when an anthropomorphic simulator (phantom) is irradiated using Exposure Computational Models (ECMs). An ECM is a virtual scene with a phantom positioned mathematically relative to a radioactive source. The initial state includes information like the type of primary particle, its energy, starting point coordinates, and direction. Subsequently, robust Monte Carlo (MC) codes are used to simulate the particle's mean free path, interaction with the medium's atoms, and energy deposition. These are common steps for simulations involving photons and/or primary electrons. The GDN (Research Group on Numerical Dosimetry and the Research Group on Computational Dosimetry and Embedded Systems) has published ECMs with voxel phantoms irradiated by photons using the MC code EGSnrc. This work has led to specific computational tools development for various numerical dosimetry stages, including input file preparation, ECM execution, and result analysis. Since 2004, the GDN developed in-house applications like FANTOMAS, CALDose_X, DIP, and MonteCarlo. Certain previously used phantoms are reintroduced to provide historical context in the ECMs' production timeline, emphasizing additive modifications inherent in systematic theme studies. The dosimetric evaluations used the binary version of the MASH (Male Adult mesh) phantom, converted to the SID (Dosimetric Information System) text file type. This format has been used by the group since 2021 to couple a voxel phantom to the EGSnrc user code. The ECM included an environmental dosimetry problem simulation. Most of these tools are accessible on the GDN page (http://dosimetrianumerica.org).

Advantages and Disadvantages of Computational Dosimetry Strategies in the Low mmW Range: Comparison Between Multilayer Slab and Anthropomorphic Models

Wireless technologies spanning at the higher bands of microwave range (i.e., Ka-band) are gaining increasing importance in everyday life. Most recent wireless power transfer (WPT) applications work in the microwave and millimeter wave (mmW) frequencies, overlapping with the new frequency bands of the fifth-generation (5G) mobile technology. The spread of such novel electromagnetic (EM) sources raised the need to investigate their possible effects on population’s health. Numerical dosimetry is fundamental to assess the exposure of the human body. In the range 24–28 GHz, i.e., the low-band spectrum of the mmW, and bridge between microwaves and mmW, there is poor consensus on the best strategy to model the human body. This article proposes a comparison between the two numerical methodologies typically adopted for this frequency range: the use of multilayer planar slabs and realistic anthropomorphic numerical models. The aim is to highlight the advantages and limits of each method, by comparing EM exposure results obtained on the Virtual Population (ViP) model Duke with several multilayer planar slabs. The differences between the two methods are non-negligible, suggesting the need of further studies and the necessity of improving both modeling approaches, depending on the frequency of work and the investigated application.

Astrocyte Responses Influence Local Effects of Whole-Brain Magnetic Stimulation in Parkinsonian Rats.

Excessive glutamatergic transmission in the striatum is implicated in Parkinson's disease (PD) progression. Astrocytes maintain glutamate homeostasis, protecting from excitotoxicity through the glutamate-aspartate transporter (GLAST), whose alterations have been reported in PD. Noninvasive brain stimulation using intermittent theta-burst stimulation (iTBS) acts on striatal neurons and glia, inducing neuromodulatory effects and functional recovery in experimental parkinsonism. Because PD is associated with altered astrocyte function, we hypothesized that acute iTBS, known to rescue striatal glutamatergic transmission, exerts regional- and cell-specific effects through modulation of glial functions. 6-Hydroxydopamine-lesioned rats were exposed to acute iTBS, and the areas predicted to be more responsive by a biophysical, hyper-realistic computational model that faithfully reconstructs the experimental setting were analyzed. The effects of iTBS on glial cells and motor behavior were evaluated by molecular and morphological analyses, and CatWalk and Stepping test, respectively. As predicted by the model, the hippocampus, cerebellum, and striatum displayed a marked c-FOS activation after iTBS, with the striatum showing specific morphological and molecular changes in the astrocytes, decreased phospho-CREB levels, and recovery of GLAST. Striatal-dependent motor performances were also significantly improved. These data uncover an unknown iTBS effect on astrocytes, advancing the understanding of the complex mechanisms involved in TMS-mediated functional recovery. Data on numerical dosimetry, obtained with a degree of anatomical details never before considered and validated by the biological findings, provide a framework to predict the electric-field induced in different specific brain areas and associate it with functional and molecular changes. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.

Assessment of Children's Exposure to Intelligent Transport System 5.9 GHz Vehicular Connectivity Using Numerical Dosimetry.

This study investigates the radio-frequency electromagnetic field exposure (RF-EMF) levels in pedestrians generated by vehicular communication technology. We specifically investigated exposure levels in children of different ages and both genders. This study also compares the children's exposure levels generated by such technology with those of an adult investigated in our previous study. The exposure scenario consisted of a 3D-CAD model of a vehicle equipped with two vehicular antennas operating at 5.9 GHz, each fed with 1 W power. Four child models were analyzed near the front and back of the car. The RF-EMF exposure levels were expressed as the Specific Absorption Rate (SAR) calculated over the whole body and 10 g mass (SAR10g) of the skin and 1 g mass (SAR1g) of the eyes. The maximum SAR10g value of 9 mW/kg was found in the skin of the head of the tallest child. The maximum whole-body SAR was 0.18 mW/kg and was found in the tallest child. As a general result, it was found that children's exposure levels are lower than those of adults. All the SAR values are well below the limits recommended by the International Commission on Non-Ionizing Radiation Protection (ICNIRP) in the general population.

Development of anthropomorphic computational phantoms at the UFPE

To evaluate the amount of energy deposited in radiosensitive organs and tissues of the human body, when an anthropomorphic phantom is irradiated, researchers in numerical dosimetry use the so-called exposure computational models (ECMs). One can imagine an ECM as a virtual scene composed of a phantom in a mathematically defined position in relation to a radioactive source. The source in these ECMs produces the initial state of the simulation: the position, direction, and energy with which each particle enters the phantom are essential variables. For subsequent states of a particle history, robust Monte Carlo (MC) codes are used. For the subsequent states of a particle's history, robust Monte Carlo (MC) codes are used, which simulate the average free path that the particle performs without interacting, its interaction with the atoms in the medium and the amount of energy deposited per interaction. MC codes also evaluate normalization quantities, so the results are printed in text files in the form of conversion coefficients between the absorbed dose and the selected normalization quantity. From the 2000s, the authors have published ECMs where a voxel phantom is irradiated by photons in the environment of the MC code EGSnrc (EGS = Electron Gamma Shower; nrc = National Research Council Canada). The production of articles, dissertations and theses required the use of specific computational tools, such as the FANTOMAS, DIP (Digital Image Processing) and Monte Carlo applications, for the various steps of numerical dosimetry, which ranges from the preparation of input files to the execution from the ECM to the organization and graphical and numerical analysis of the results. This article reviews computational phantoms for dosimetry mainly those produced in DEN-UFPE dissertations and thesis.

Monte-Carlo Based Numerical Dosimetry in Reverberation Chamber Exposure Systems Employed for in-Vivo Rodent Bioassays

A Monte-Carlo based computational approach for the statistical characterization of the whole-body specific absorption rate (wbSAR) variability in large cohorts of rodents exposed to radio-frequency (RF) energy in reverberation chambers (RCs) is applied to adult male rat exposures illustrative of those in a US National Toxicology Program (NTP) cancer bioassay. A large number of 3D electromagnetic field realizations fulfilling Rayleigh fading properties were generated within an electrically-large volume representative of an ideal RC, yielding granular wbSAR distributions for an ensemble of 96 homogeneous rodent models with random mass distribution, postures, positions and orientations. Two case studies were addressed: a “momentary exposure” with each rat fixed in posture, position and orientation, and a “day-long exposure” in which the position, orientation and posture were varied randomly for each subsequent Rayleigh field realization. Over 500 and 2500 field realizations or “snapshots”, respectively, the rats’ instantaneous wbSARs, as well as their individual time-averaged wbSARs, were found to be well fit by lognormal distributions. The large variability in instantaneous wbSARs in the cohort was due in part to the inherent Rayleigh field variability in RCs (70-80%) and in part to weight, posture and position variations (20-30%), while the effect of cage location was found to be small over day-long exposures. Averaging the exposure over field realizations substantially reduces the range of wbSARs in the cohort. Hence, when RF-induced thermal effects are studied, the relevant exposure metric (wbSAR averaged over appropriate times) features a narrower range than instantaneous wbSAR, which is the relevant metric in studies dealing with non-thermal effects. Compared to previous studies, the present approach was found to be computationally more efficient enabling thus a Monte-Carlo analysis by varying concurrently the incident field and the animals posture, position, and orientation. In practice, it can inform the choice of wbSAR targets in rodent bioassay, allowing to identify possible dose-effect trends while avoiding undue thermal stress.

Estimation method for the anisotropic electrical conductivity of in vivo human muscles and fat between 10 kHz and 1 MHz

Objective. In low frequency dosimetry the variability in the electrical conductivity values assigned to body model tissues represents a major source of uncertainty. The aim of this study is to propose a method for estimating the conductivity of human anisotropic skeletal muscle and fat in vivo in the frequency range from 10 kHz to 1 MHz. Approach. A method based on bounded electrical impedance tomography was used. Bioimpedance measurements were performed on the legs of ten subjects. Anatomically realistic models of the legs were then created using magnetic resonance images. The inverse problem of the tissue conductivities was solved using the finite element method. The results were validated using resampling techniques. These findings were also used to study the effects of muscle anisotropy on magnetic field exposure. Main results. The estimated conductivities for anisotropic muscle were found to be in good agreement with values found in existing literature and the anisotropy was shown to decrease with increasing frequency, with the ratio of lateral to longitudinal conductivity increasing from 37% to 64%. The conductivity of fat was found to be almost a constant 0.07 S m−1 in the frequency range considered. Significance. The proposed method was shown to be a viable option when estimating in vivo conductivity of human tissue. The results can be used in numerical dosimetry calculations or as limits in future investigations studying conductivity with bioimpedance measurements.

Pilot study of a new methodology to study the development of the blue bottle fly (Calliphora vomitoria) under exposure to radio-frequency electromagnetic fields at 5.4 GHz

Purpose Exposure of insects to radio-frequency electromagnetic fields (RF-EMFs) can have developmental effects. However, there is currently no clear understanding of the exposure level that can lead to such effects. Therefore, the goal of this study was to, for the first time, study the development of the Blue Bottle Fly (Calliphora vomitoria, CV) under exposure to RF-EMFs at 5.4 GHz, using both numerical RF-EMF dosimetry with anatomically accurate 3 D models of insects and an RF-EMF exposure experiment. Materials and Methods CV was chosen as a model organism in this study because CV’s development can be influenced thermally and CV’s pupal stage presents a window of several days in which immobile pupae can be exposed to RF-EMFs. The 5.4 GHz frequency was used because it allowed us the license-free operation of the exposure setup. Numerical, EM simulations with 3 D anatomically accurate models of CV, obtained using micro-CT scanning, were used in this study. These simulations enable the estimation of the absorbed power and the whole-body averaged specific absorption rate in CV during RF exposure experiments. An experiment with three exposure conditions was designed and executed in which 400 pupae were split into an exposed group that was placed inside the TEM cell for 48 h and concurrent control. Two exposure conditions used RF-EMF input power into the TEM cell at 5.4 GHz on two different levels. One exposure condition was sham exposure. Electric field strength measurements were used to validate the proper functioning of the exposure setups and to quantify the RF-EMF exposure of the control groups. Results and conclusions All studied groups of pupae – exposed to RF-EMFs, sham, and control groups- showed similar (evolutions of) masses, lengths and diameters during their development. The total rate of pupal emergence was reduced in one of the studied RF-EMF exposures in comparison to its concurrent control, while the other RF-EMF exposure and the sham exposure did not alter the total rate of pupal emergence. The sham exposure and the lowest of the two studied RF-EMF exposure conditions (19.4 V/m) caused a median delay in pupal emergence of 4 and 8 hours, respectively, in comparison to concurrent control groups. The higher studied exposure of 55 V/m caused a median relative acceleration in the development of 8 h.

Quantification of Exposure Level in a Reverberation Chamber for a Large-Scale Animal Study

The US National Toxicology Program (NTP) conducted a long-term carcinogenic and toxicity study with small animals on the effects of mobile phone radiation at 900 MHz and 1900 MHz, respectively, and showed an increase in incidence of malignant schwannomas in the heart of male rats at very higher exposure levels. To verify and clarify the NTP study results, Japan and Korea are conducting a joint study using the same reverberation chamber type exposure system as in the NTP study. The purpose of this paper is to derive a quantitative relationship between the electric field strength in the reverberation chamber and the exposure level, i.e., the mean whole-body averaged specific absorption rate (WBA <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\_$</tex-math></inline-formula> SAR) of rats based on a large-scale finite difference time domain (FDTD) simulation. First, two FDTD simulation methods, one with a single plane wave incidence and the other with simultaneous plane wave incidence, were compared, and then the former method was adopted for convenience and saving calculation time. The derived quantitative relationship between the WBA <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\_$</tex-math></inline-formula> SAR and the electric field strength required to achieve the WBA <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\_$</tex-math></inline-formula> SAR consists of a one-dimensional approximation model which considers only the body mass of rats and a two-dimensional approximation model which considers not only the body mass but also the number of rats. The two approximation models cover the entire two-year exposure period, and the exposure level in the two-year long-term exposure experiment is being well controlled. Moreover, unlike dosimetry in the NTP study, an uncertainty analysis of numerical dosimetry was performed in more details, and the combined standard uncertainty in the WBA <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\_$</tex-math></inline-formula> SAR was found to be 16.1 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\%$</tex-math></inline-formula> .

Analysis of Numerical Artifacts Using Tetrahedral Meshes in Low Frequency Numerical Dosimetry

Anatomical realistic voxel models of human beings are commonly used in numerical dosimetry to evaluate the human exposure to low-frequency electromagnetic fields. The downside of these models is that they do not correctly reproduce the boundaries of curved surfaces. The stair-casing approximation errors introduce computational artifacts in the evaluation of the induced electric field and the use of post-processing filtering methods is essential to mitigate these errors. With a suitable exposure scenario, this paper shows that tetrahedral meshes make it possible to remove stair-casing errors. However, using tetrahedral meshes is not a sufficient condition to completely remove artifacts, because the quality of the tetrahedral mesh plays an important role. The analyses carried out show that in real exposure scenarios, other sources of artifacts cause peak values of the induced electric field even with regular meshes. In these cases, the adoption of filtering techniques cannot be avoided.

In Vivo Functional Ultrasound (fUS) Real-Time Imaging and Dosimetry of Mice Brain Under Radiofrequency Exposure.

This study aims to analyze in real-time the potential modifications induced by low-level continuous-wave and Global System for Mobile Communications radiofrequency (RF) exposure at 1.8 GHz on brain activation in anesthetized mice. A specific in vivo experimental setup consisting of a dipole antenna for the local exposure of the brain was fully characterized. A unique neuroimaging technique based on a functional ultrasound (fUS) probe was used to observe the areas of mice brain activation simultaneously to the RF exposure with unprecedented spatial and temporal resolution (~100 μm, 1 ms) following manual whisker stimulation using a brush. Numerical and experimental dosimetry was carried out to characterize the exposure and to guarantee the validity of the biological results. Our results show that the fUS probe can be efficiently used during in vivo exposure without interference with the dipole. In addition, we conclude that exposure to brain-averaged specific absorption rate levels of 2 and 6 W/kg does not introduce significant changes in the time course of the evoked fUS response in the left barrel field cortex. The proposed technique represents a valuable instrument for providing new insights into the possible effects induced on brain activation under RF exposure. For the first time, brain activity under mobile phone exposure was evaluated in vivo with fUS imaging, paving the way for more realistic exposure configurations, i.e.awake mice and new signals such as the 5 G networks. © 2022 Bioelectromagnetics Society.

Dosimetry of Various Human Bodies Exposed to Microwave Broadband Electromagnetic Pulses

In this paper, human exposures to ultra-wideband (UWB) electromagnetic (EM) pulses in the microwave region are assessed using a frequency-dependent FDTD scheme previously proposed by the authors. Complex permittivity functions of all biological tissues used in the numerical analyses are accurately expressed by the four-term Cole–Cole model. In our method, we apply the fast inverse Laplace transform to determine the time-domain impulse response, utilize the Prony method to find the Z-domain representation, and extract residues and poles for use in the FDTD formulation. Update equations for the electric field are then derived via the Z-transformation. Firstly, we perform reflection and transmission analyses of a multilayer composed of six different biological tissues and then confirm the validity of the proposed method by comparison with analytical results. Finally, numerical dosimetry of various human bodies exposed to EM pulses from the front in the microwave frequency range is performed, and the specific energy absorption is evaluated and compared with that prescribed in international guidelines.

Exposure Assessment of Array Antennas at 28 GHz Using Hybrid Spherical Near-Field Transformation and FDTD Method

To protect humans from overexposure to electromagnetic (EM) fields above 6 GHz, recently revised International Commission on Non-Ionizing Radiation Protection guidelines and IEEE standard specify the basic restrictions in terms of absorbed power density (APD) and epithelial power density, respectively. To assess the EM exposure resulting from the 5G mmWave array antenna, we adopted a hybrid method that combines spherical near-field measurements and numerical dosimetry with spherical near-field transformation. Four array antennas working at 28 GHz were used as the radiation sources. The incident power density (IPD), APD, and temperature rise in a stratified body model and anatomical partial body models corresponding to different body locations were evaluated for various antenna-body distances. The results were validated by comparison with the full-wave simulations. The differences in peak spatial-average IPD and APD between two methods are below 0.08 and 1.05 dB, respectively, for the simulated cases and generally decrease with the increasing antenna-body distance. The highest heating factor for the peak spatial-average APD was approximately 0.025°C/(W/m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ). The results also show that the differences in APD and temperature rise caused by various exposed body locations are marginal.

Chassis Influence on the Exposure Assessment of a Compact EV during WPT Recharging Operations

In this study, the external magnetic field emitted by a wireless power transfer (WPT) system and the internal electric field induced in human body models during recharging operations of a compact electric vehicle (EV) are evaluated. The magneticfield is calculated with a hybrid scheme coupling the boundary element method with the surface impedance boundary conditions in order to fit the multiscale open-boundary characteristics of the problem. A commercial software is then used to perform numerical dosimetry. Specifically, two realistic anatomical models, both in a driving position and in a standing posture, are considered, and the chassis of the EV is modeled either as a currently employed aluminum alloy and as a futuristic carbon fiber composite panel. Aligned and misaligned coil configurations of the WPT system are considered as well. The analysis of the obtained results shows that the International Commission on Non-Ionizing Radiation Protection (ICNIRP) reference levels are exceeded in the driving position, especially for the carbon fiber chassis, whereas the system is compliant with the basic restrictions, at least for the considered scenarios.

Possible effects of different doses of 2.1 GHz electromagnetic radiation on learning, and hippocampal levels of cholinergic biomarkers in Wistar rats

ABSTRACT The present study evaluated whether short-term exposure to different doses of 2.1 GHz radiofrequency electromagnetic radiation (RF-EMR) has different effects on rats’ behaviour and hippocampal levels of central cholinergic biomarkers. Animals were divided into three equal groups namely; group 1 was sham-exposed group, group 2–3 were exposed to 45 V/m and 65 V/m doses of 2.1 GHz frequency for 1 week respectively. Numerical dosimetry simulations were carried out. Object location and Y-maze were used as behavioural tasks. The protein and mRNA expression levels of AChE, ChAT, and VAChT, in the hippocampus were tested using Western Blotting and Real-Time PCR. The impairment performance of rats subjected to 65 V/m dose of 2.1 GHz RF-EMR in both object location and Y-maze tasks was observed. The hippocampal levels of AChE, ChAT, and VAChT, were significantly lower in rats exposed to 65 V/m dose of 2.1 GHz RF-EMR than others. The stronger effect of “65 V/m” dose on both rat’s hippocampal-dependent behavioural performances and hippocampal levels of cholinergic biomarkers may be due to the stronger effect of “65 V/m” dose where rats’ snouts were located at the nearest distance from the monopole antenna. Furthermore, the simulated SAR values were high for 65 V/m electric-field strengths. For the first time, we report the potential dose-dependent effects of short-term exposure to 2.1 GHz radiation on rat’s behavioural performances as well as hippocampal levels of cholinergic biomarkers. Further studies are needed to understand the mechanisms by which RF-EMR influences the function of the central cholinergic system in the brain.