We assessed potential ecotoxicity of lake sediments affected by biomining effluents in northeastern Finland. Growth, reproduction and behavior of the sediment-dwelling oligochaete Lumbriculus variegatus (Müller 1774) were used as ecotoxicity endpoints. Standardized chronic bioassays were used for growth and reproduction, and acute and chronic tests with Multispecies Freshwater Biomonitor (MFB) for behavior assessments. Sequential extractions were used to characterize metal bioavailability and exposure conditions in the sediments, which indicated mining-induced contamination gradients of S, Cu, Ni and U and also bioavailability gradients of S and Ni. Among the ecotoxicity endpoints, growth and reproduction responses of the standard bioassays appeared more sensitive than the behavioral responses at 21 d. In the two most mining-affected test sediments, mean number of worms and dry biomass decreased 35–42% and 46–51% in comparison to the reference sediment, respectively. The behavioral changes of worms, i.e. peristaltic and overall locomotory activity, decreased on average 20–70% and 2–61% at 21 d in the same sediments. However, these behavioral changes were observed at the onset of exposure indicating MFB technique is a suitable and rapid screening level ecotoxicity assessment tool.