Plant nitrogen concentration (PNC) is a traditional standard index to measure the nitrogen nutritional status of winter wheat. Rapid and accurate diagnosis of PNC performs an important role in mastering the growth status of winter wheat and guiding field precision fertilization. In this study, the in situ hyperspectral reflectance data were measured by handheld SVC HR−1024I (SVC) passive field spectroradiometer and PNC were determined by the modified Kjeldahl digestion method. Continuous wavelet transform (CWT), successive projection algorithm (SPA) and partial least square (PLS) regression were combined to construct an efficient method for estimating winter wheat PNC. The main objectives of this study were to (1) use CWT to extract various wavelet coefficients under different decomposition scales, (2) use SPA to screen sensitive wavelet coefficients as independent variables and combine with PLS regression to establish winter wheat PNC estimation models, respectively, and (3) compare the precision of PLS regression models to find a reliable model for estimating winter wheat PNC during the growing season. The results of this paper showed that properly increasing the decomposition scale of CWT could weaken the impact of high-frequency noise on the prediction model. The number of wavelet coefficients has been significantly reduced after screened by SPA. The PNC estimation model (CWT–Scale6–SPA–PLS) based on the wavelet coefficients of the sixth decomposition scale most accurately predicted the PNC (the determination coefficient of the calibration set (Rc2) was 0.85. Root mean square error of the calibration set (RMSEc) was 0.27. The determination coefficient of the validation set (Rv2) was 0.84. Root mean square error of the validation set (RMSEv) was 0.28 and relative prediction deviation (RPD) was 2.47). CWT-Scale6-SPA-PLS can be used to predict PNC. The optimal winter wheat PNC prediction model based on CWT proposed in this study is a reliable method for rapid and nondestructive monitoring of PNC and provides a new technical method for precision nitrogen management.
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