Number Of Blood Platelets Research Articles

Standardized bone marrow assessment, risk variables, and survival in dogs with myelodysplastic syndrome and acute myeloid leukemia.

Myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) are heterogeneous neoplasms of hematopoietic stem cells that are challenging to diagnose, differentiate, and prognosticate. Cytogenetic and mutational analyses are useful in humans but unavailable for dogs, where diagnosis and classification still rely largely on hematologic and morphologic assessment. The objectives of this study were to apply a classification scheme to myeloid neoplasms and to assess outcome in relation to predictor variables. Keyword search of a laboratory database, application of sequential exclusion criteria, and consensus from 3 reviewers yielded 70 cases of myeloid neoplasia with hematology results, and cytologic (11), histologic (14), or both (45) types of marrow specimens. Based on blast percentage and morphology, 42 cases were classified as MDS and 28 as AML. Dogs with MDS had significantly lower body weights, hemoglobin concentrations and blood blasts, and higher red blood cell size variability and platelet numbers than dogs with AML. Estimates of median survival using Kaplan-Meier curves for dogs with MDS and AML were 384 and 6 days, respectively (P < .001). The instantaneous risk of death for dogs with MDS was approximately 5× lower than that of dogs with AML. Significant predictor variables of survival were body weight, white blood cell count, platelet count, and percent blood blasts (P < .05). Hazard ratios (HRs) derived from best-fitting Cox regression models were 1.043, 0.998, and 1.061 for increased neutrophils, decreased platelets, and increased blood blasts, respectively. Findings from this study suggest that hematologic and morphologic variables are useful to predict outcomes in myeloid neoplasia.

Exploring Serum Copeptin and Hematological Profile: A Comparative Analysis after Intradermal versus Intramuscular Porcine Reproductive and Respiratory Syndrome Virus Vaccination in Piglets

This study aimed to investigate the impact of intradermal (ID) and intramuscular (IM) vaccination with a porcine reproductive and respiratory syndrome virus (PRRSV)-modified live vaccine (MLV) in piglets on serum copeptin levels and hematological profile. This study included 104 suckling piglets (2 weeks of age) from a commercial farrow-to-finish pig farm suffering from positive unstable PRRSV status. Animals were assigned to four groups, with two replicates (13 piglets/group/replicate); group A: IM vaccination with a PRRSV MLV vaccine, group B: ID vaccination with the same vaccine, group C: ID of Diluvac Forte, and group D: IM of Diluvac Forte. Blood samples were collected from the same three pigs/group/replicate at 4, 7, and 10 weeks of age. Blood samples were used for the performance of the complete blood count, and they were also examined by PCR for PRRSV and by ELISA for copeptin. No significant differences in serum copeptin levels and the number of blood cell counts (packed cell volume—PCV, numbers of white blood cells—WBCs, and platelets number—PLTs) were noticed in the same group over time and among groups. In conclusion, it seems that the vaccination against PRRSV does not affect the levels of the released copeptin. Based on our results, the measurement of serum copeptin could not be proposed as a potential stress biomarker in pigs.

The ameliorative effect on chemotherapy-induced injury and tumor immunosuppressive microenvironment of the polysaccharide from the rhizome of Menispermum dauricum DC

Combined medication has attracted increasing attention as an important treatment option for tumors due to the serious adverse effects of chemotherapy. In this study, as a new therapy strategy, a combination treatment of MDP (a polysaccharide from the rhizome of Menispermum dauricum DC.) with cyclophosphamide (CTX) was investigated. The results showed that combination treatment with MDP and CTX exerted a significantly synergistic anti-tumor effect in Lewis tumor-bearing mice, improved CTX-induced emaciation and hair loss, as well as increased the number of leukocytes, erythrocytes, hemoglobin, and platelets in the peripheral blood. In addition, compared with CTX alone, the thymus index and spleen index of the MDP + CTX group were increased, the number of CD3 + T cells, CD8 + T cells, white blood cells and B cells in spleen also increased significantly. MDP could also ameliorate the increase in liver and kidney index caused by CTX. In the Lewis lung cancer model, MDP showed a certain degree of anti-tumor effects, which may be related to its promotion of tumor-associated macrophages (TAMs) to M1 phenotype polarisation, enhancement of the number of T cells in tumor tissues and promotion of Th cells in tumor tissues to Th1 phenotype polarisation, thus alleviating the immunosuppressive microenvironment in tumor tissues. This study laid the foundation for the development of MDP as a polysaccharide drug for the treatment or adjuvant therapy of tumors and has important significance for the further clinical application of polysaccharides.

Purified Granulocyte Concentrates from Buffy Coats with Extended Storage Time

Background: Granulocyte concentrates (GCs) are usually prepared by single-donor apheresis after G-CSF pretreatment and have to be transfused within 24 h after cell collection because of the rapid decrease in pH and cell survival due to high lactate production by red blood cell contamination. GCs pooled from buffy coats of whole blood donations could improve the availability of these products. Methods to reduce red blood cell and platelet contamination may improve storability. We developed a manufacturing process for pooled GCs and investigated cell viability and functionality over time. Methods: Six ABO blood group-identical buffy coats were pooled. Subsequently, the red blood cells spontaneously sedimented after the addition of hydroxyethyl starch. The resulting leukocyte-enriched supernatant was washed twice with saline to reduce platelets and was resuspended in ABO-identical donor plasma. The leukocyte concentrate was transferred to a platelet storage bag and stored up to 72 h at 20–24°C w/o agitation. Cell count and viability, pH, blood gases, phagocytosis, and oxidative burst activity were monitored. Results: The number of red blood cells and platelets was reduced to 0.4% and 6.1% of the baseline levels. About 50% of the original present leukocytes could be extracted (n = 76). In the course of 72 h of storage, there were no significant changes in white blood cell counts (p = 0.12). The viability exceeded 98% during the entire period. The rate of granulocytes performing phagocytosis and oxidative burst remained above 95% anytime. Conclusion: GCs prepared from pooled buffy coats provide a precious alternative to granulocytes obtained from apheresis. Reduction of red blood cells and platelets by more than 90% extends the maximum shelf life of GCs from 24 h to 72 h. For a therapeutic dose of at least 1 × 1010 granulocytes, 15–20 buffy coats are required.

Evaluation of hematological and blood biochemical indices in cultured Nile tilapia (Oreochromis niloticus) as affected by using phage therapy against Pseudomonas aeruginosa

Abstract Pseudomonas spp. causes significant losses in aquaculture, consecutive use of antibiotics, and reveals bacterial resistance; therefore, therapeutic bacteriophages, commonly called phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms, including cultured fish. The novelty of current work is represented in examining the lytic activity of four phages and their combination compared to the antibiotic streptomycin on Pseudomonas aeruginosa-infected Nile tilapia (Oreochromis niloticus) while measuring the hematological and blood biochemical parameters as a response for phage therapy. This study evaluated the in vitro killing curve for each phage using a growth curve that measured the OD600 after a single phage suspension was combined with the host P. aeruginosa, considered the best multiplicity of infection (MOI) for each phage. A144 healthy fish were acclimatized in the laboratory and divided into six groups: control, P. aeruginosa-infected fish, streptomycin, phage Ps1, Ps2, both (Ps1 and Ps2), were added to the T3, T4, T5, and T6 groups, respectively. Our findings demonstrated that P. aeruginosa infection caused surface body hemorrhages, tail and fin rot, irritated skin, superficial ulcers, and 100% mortality through 14 days. P. aeruginosa caused a reduction in hemoglobin (Hb), red blood cells (RBCs), platelet number (PLt), and platelet crit (PCT) count, protein, albumin, and A/G ratio; however, an increase in hematocrit (Hct), red cell distribution width (RDW), PDW, MPV compared to other groups after three days of infection and the effects increased after 12 days post-infection. The fish vaccinated with P1 (T4) and P1+P2 (T6) showed enhanced levels of Hb, RBCs, PLt, PCt, protein, albumin and decreased levels of RDW, PDW, MPV, and liver and kidney enzymes with enhanced contents more than streptomycin and closer to the control group. The biochemical markers recorded significant changes indicating liver and kidney impairments due to the infection with P. aeruginosa. It can be concluded that P1 and P1+P2 combinations could be used as therapy in Pseudomonas-infected fish to enhance their blood parameters and performance.

New light on changes in the number and function of blood platelets stimulated by cocoa and its products.

Hyperactivation of blood platelets, one of the causes of heart attack, and other cardiovascular diseases (CVDs), is influenced by various dietary components, including phenolic compounds from vegetables, fruits, teas, wines, cocoa and its products, including chocolate. The present paper sheds new light on the effect of cocoa and its products, especially dark chocolate, on the number and function of blood platelets, and the anti-platelet activity of their constituent phenolic compounds. A review was performed of papers identified in various electronic databases, including PubMed, Science Direct, Scopus, Web of Knowledge, and Google Scholar, with the aim of determining whether their anti-platelet activity may serve as part of a sweet strategy in countering CVDs. Various studies demonstrate that cocoa consumption, especially in the form of dark chocolate, with a high flavanol concentration, has anti-platelet activity and may play a significant role in cardioprotection; they also note that cocoa consumption may be a good strategy in diminishing cardiovascular risk, including hyperactivation of blood platelets.

Genetic background of hematological parameters in Holstein cattle based on genome-wide association and RNA sequencing analyses

Hematological parameters refer to the assessment of changes in the number and distribution of blood cells, including leukocytes (LES), erythrocytes (ERS), and platelets (PLS), which are essential for the early diagnosis of hematological system disorders and other systemic diseases in livestock. In this context, the primary objectives of this study were to investigate the genomic background of 19 hematological parameters in Holstein cattle, focusing on LES, ERS, and PLS blood components. Genetic and phenotypic (co)variances of hematological parameters were calculated based on the Average Information Restricted Maximum Likelihood (AIREML) method and 1,610 genotyped individuals and 5,499 hematological parameter records from 4,543 cows. Furthermore, we assessed the genetic relationship between these hematological parameters and other economically important traits in dairy cattle breeding programs. We also carried out genome-wide association studies and candidate gene analyses. Blood samples from 21 primiparous cows were used to identify candidate genes further through RNA sequencing (RNA-seq) analyses. Hematological parameters generally exhibited low-to-moderate heritabilities ranging from 0.01 to 0.29, with genetic correlations between them ranging from -0.88 ± 0.09 (between mononuclear cell ratio and lymphocyte cell ratio) to 0.99 ± 0.01 (between white blood cell count and granulocyte cell count). Furthermore, low to moderate approximate genetic correlations between hematological parameters with one longevity, 4 fertility, and 5 health traits were observed. One-hundred-and-99 significant single nucleotide polymorphisms (SNP) located primarily on the Bos taurus autosomes (BTA) BTA4, BTA6, and BTA8 were associated with 16 hematological parameters. Based on the RNA-seq analyses, 6,687 genes were significantly downregulated and 4,119 genes were upregulated when comparing 2 groups of cows with high and low phenotypic values. By integrating genome-wide association studies (GWAS), RNA-seq, and previously published results, the main candidate genes associated with hematological parameters in Holstein cattle were ACRBP, ADAMTS3, CANT1, CCM2L, CNN3, CPLANE1, GPAT3, GRIP2, PLAGL2, RTL6, SOX4, WDFY3, and ZNF614. Hematological parameters are heritable and moderately to highly genetically correlated among themselves. The large number of candidate genes identified based on GWAS and RNA-seq indicate the polygenic nature and complex genetic determinism of hematological parameters in Holstein cattle.

Liposomes Enhance the Immunological Activity of Polygonatum Cyrtonema Hua Polysaccharides

Poor stability and difficult uptake of natural polysaccharides have been the main problems in their application. The purpose of this study was to optimize the preparation conditions of Polygonatum cyrtonema Hua polysaccharides liposomes (PCPL) and to investigate the immune enhancement activity of PCPL in vitro and in vivo, with a view to discovering new ways of natural polysaccharide application. The optimal preparation conditions of PCPL were as follows: the adding amount of Tween 80 of 0.5 %, the ultrasound time of 2 min and the ultrasound times of once. Under these conditions, the entrapment efficiency, drug loading rate and particle size of PCPL were 38.033 %±0.050, 2.172 %±0.003 and 146 nm, which indicated that PCPL with small particle size could be prepared by the reverse-phase evaporation method. Furthermore, PCPL promoted proliferation, phagocytosis, and secretion of nitric oxide and related cytokines in RAW264.7 cells. Moreover, PCPL improved spleen and thymus indices, increased the number or proportion of red blood cells, platelets, and lymphocytes in the blood, and ameliorated spleen and thymus atrophy in immunosuppressed mice. This study provides a new idea for applying Polygonatum cyrtonema Hua polysaccharides (PCP) and references for studying other polysaccharides.

The Relationship Of Lead (Pb) Levels With The Number Of Blood Platelets At SPBU Officers

Lead (Pb) is the main pollutant in the environment. Lead in fuel is an additive that is added to a gasoline mixture with the aim of increasing combustion so that vehicle performance increases. Lead is a type of heavy metal that can cause poisoning. Lead is easily absorbed in the body and can accumulate in human tissues. Lead can enter the human body through the respiratory system, orally, or through the skin surface. High lead levels in the blood can cause low platelets. This is because lead can interfere with the heme system in the blood, causing anemia. The aim of the study was to determine the relationship between lead (Pb) levels and blood platelet counts at gas stations in Mekikis Village. The research design in this study used a cross sectional approach. The research sample consisted of 10 gas station attendants. The sampling technique used was purposive sampling. Based on the examination, the average lead and platelet levels for gas station workers were 0.4344 ppm and 293,500 /UL respectively. Based on the results of the Spearman correlation test, a significant value of 0.391 was obtained where the sign value was &gt; 0.05, which means that there is no relationship between lead levels and the number of platelets in the blood of Mekikis Village gas station attendants.

Targeting the JAK2V617F Hematopoietic Stem Cell through Beta1 Integrin

The major barrier to progress in treatment of primary myelofibrosis (PMF) is the lack of effective therapies targeting the malignant JAK2 V617F hematopoietic stem cell (HSC), the source of disease progression and relapse. Expression of JAK2 V617F induces abnormal proliferation and differentiation of HSC, affecting all blood lineages but most prominently the erythro-megakaryocytic lineage, leading to clonal megakaryocyte proliferation, erythrocytosis and thrombocytosis. In our previously published work ( Matsuura et al, Blood 2020) we identified elevated expression of the α5 subunit of the α5β1 integrin receptor as a driver of JAK2 V617F megakaryocyte proliferation. Expression of β1 integrin subunit was similar in wild-type (WT) and JAK2 V617F megakaryocytes, but activation of β1 integrin, detected by the conformation-specific 9EG7 antibody, was elevated in JAK2 V617F megakaryocytes. Following up on those findings, we detected elevated β1 integrin activation in long-term hematopoietic stem cells (HSC LT, LSKCD48-CD150+) from JAK2 V617F transgenic mice ( Xing et al, Blood 2008) when compared to WT controls. To determine whether inhibition of β1 integrin in vivo reduces the number of HSC LT in JAK2 V617F transgenic mice, we examined HSC LT 24 hours after intravenous administration of 2mg/Kg of anti-β1 integrin inhibitory antibody HMβ1-1. This regimen led to a sharp reduction in number and percentage of HSC LT in JAK2 V617F transgenic mice, to 45% of control antibody-treated animals. The effect on WT HSC LT was negligible. HSC ST (LSKCD48-CD150-) and MPP2 (LSKCD48+CD150+) were also reduced, but the reduction was not statistically significant. MPP3/4 (LSKCD48+CD150-) and LSK were not affected. Megakaryocyte progenitors (LKCD41+CD150+) were significantly reduced. Inhibition of β1 integrin also sharply reduced peripheral blood platelet numbers in WT animals. The reduction in JAK2 V617F transgenic mice was not statistically significant. A longer observation time of 7 days demonstrated that while WT animals were able to mount a robust regenerative response with slight but significant increases in HSC LT and normalization of platelet numbers, in JAK2 V617F transgenic mice the number of HSC LT remained depressed, as did downstream stem/progenitor populations at various significance levels. To gain insight into the mechanisms that lead to reduction in HSC LT, we examined apoptosis, cell cycle and HSC mobilization, all processes known to be affected by β1 integrin. Although basal expression of the apoptotic marker annexin V was decreased in JAK2 V617F HSC LT compared to WT, no differences were detected upon administration of HMβ1-1 in both genotypes at 4 hours post-administration. Similarly, peripheral blood colony assays revealed a basally higher level of stem/progenitor mobilization in JAK2 V617F, which did not significantly increase upon HMβ1-1 administration. In contrast, the cell cycle profile, examined at 4 hours post-administration to account for the earliest effects, demonstrated a decrease in G0 phase cells and increase in S/G2/M cells indicating cell cycle induction and exit from quiescence in both WT and JAK2 V617F HSC LT. The results indicate that inhibition of β1 integrin disrupts the adhesion-mediated cell cycle suppression in both WT and JAK2 V617F HSC LT leading to exit from quiescence. However, the downstream consequences of cell division in HSC LT differ significantly, with an apparent loss of regenerative potential in JAK2 V617F HSC LT, at least at the observed timepoints. Longer observation times are required to determine whether continuous β1 integrin inhibition can lead to JAK2 V617F HSC LT exhaustion. More sensitive assays to monitor mobilization are warranted to quantify possible minute amounts of mobilized HSC LT. Our preclinical studies indicate that β1 integrin is an attractive target to specifically reduce the number of JAK2 V617F HSC LTin vivo. The role of β1 integrin in HSC LT has not been thoroughly studied due to lack of phenotype in β1 integrin-deficient HSC ( Brakebush et al, Immunity 2002). Our studies using the anti-β1 integrin antibody in WT HSC LT confirm previous findings. However, β1 integrin seems to be critical for maintenance of JAK2 V617F HSC LT, offering a possible therapeutic window. Understanding how β1 integrin supports quiescence in HSC LT will bring us a step closer to understanding the mechanisms that drive malignancy and fitness in JAK2 V617F HSC LT.

Effect of Drainage with Flax Oil on Weight and some Blood Characteristics During Late Pregnancy Stage of Awassi Ewes

The goal of this research was to determine the effects of various doses of flaxseed oil on ewe body weight, growth characteristics, and selected biochemical and hormonal blood characteristics. This research was carried out in the animal science department at the University of Tikrit’s College of Agriculture between October 6th, 2022, and September 1st, 2022. There were a total of 17 female Awassi sheep, ages 1-2, with an average live weight of 56.41 kg, and they were randomly assigned to one of three groups. To the ewes, flaxseed oil was administered at a rate of 0%, 6%, and 8% of the dry matter weight in the three different groups. The ewes were grazing daily, in addition to providing a supplementary diet at the level of 2.5% of body weight, consisting of 14.98 protein and 14.22 MJ/kg dry matter as energy. At the end of the experiment, there were no significant differences (P>0.05) between the treatments in ewes’ weights, the percentage of packed blood cells, the number of white blood cells and platelets, and the values of total protein, albumin, urea, and creatinine, while the control treatment was the least significant (P≤0.05). of the second and third treatments, which had the highest numbers of red blood cells (5.79, 6.61 and 7.72) 106cells/ml3 and hemoglobin (9.06, 9.80 and 10.20) mg/dl, respectively. While a significant decrease (P≤0.05) was, observe for the activity of AST, ALT and triglyceride enzymes for the third treatment compared to the control treatment. The third treatment was significantly superior (P≤0.05) in the value of blood globulin, glucose and cholesterol 1.90 g/dl, 66.66 and 84.00 mg/dl, respectively.

Correlation analysis of blood biochemical and morphological markers in small breeds dogs with obesity

Purpose : the study of biochemical and morphological indicators of the blood of dogs of small breeds with obesity depending on age, breed and weight. Materials and methods . The analysis of biochemical and morphological indicators of blood 21 dogs of small breeds in the summer-autumn period was analyzed. The age distribution was from 3 to 13 years. In the blood serum, the level of total protein, albumin, globulin, urea, creatinine, total calcium, phosphorus, the activity of aspartateataminostrostrostrase (asat), alanineine -orgrase (alat), alkaline phosphatase were determined. In stabilized blood, indicators of hematocrit, hemoglobin, the number of red blood cells, leukocytes and platelets were determined. The determination of blood indicators was carried out according to generally accepted methods. Results . Higher indicators of the activity of asat enzymes and alkaline phosphatase in the absence of a significant increase in the activity of Alat, the activity of which will increase primarily with the liver damage, were identified. When evaluating red blood indicators, the most visual change was a decrease in the level of hemoglobin and hematocrit, both with the course of age and with an increase in body weight. This may indicate inhibition of hematopoietic function in animals with increased fat mass of the body, possibly due to chronic prolonged inflammation as a result of the release of inflammatory adipokins with fat tissue. The chronic damaging effect of the presumably adipokins of adipose tissue, expressed in the non -specific increase in the activity of blood serum enzymes, anemia of chronic inflammation and moderate leukocytosis in the absence of obvious clinical signs. All of these factors have a negative impact on the work of all organs and systems, including reproductive, as well as significantly reduce the quality and life expectancy of dogs.

STUDY OF THE ROLE OF HEMOCOAGULATION DISBALANCE AS A KEY PATHOGENETIC PREDICTORS IN THE FORMATION OF SYSTEMIC DISORDERS IN EXPERIMENTAL PERITONITIS

Background. Peritonitis today continues to be one of the most difficult problems of both abdominal surgery and theoretical pathophysiology. One of the leading pathogenetic mechanisms of peritonitis is disorders in the hemostasis system and the development of dissemination vascular syndrome. Blockade of microcirculation caused by the development of dissemination vascular syndrome leads to tissue hypoxia and, as a result, to dysfunction of organs and body systems.&#x0D; Aim: is to study changes in vascular-platelet and coagulation hemostasis under conditions of experimental fecal peritonitis in rats.&#x0D; Materials and methods. Experimental studies were conducted on 24 non-linear laboratory rats, which were divided into 2 groups: 1 group – intact control (animals received distilled water), 2 group – animals of the control pathology group. According to the "Methodological recommendations for preclinical study of medicinal products", experimental peritonitis was studied on the model of V. A. Lazarenko. Indicators of vascular-platelet and coagulation hemostasis were determined according to generally accepted methods.&#x0D; Results. On the first day of experimental peritonitis, a sharp increase in the rate of blood clotting and changes in the quality properties of platelets were noted, affecting their aggregation ability and retraction of an already formed blood clot. On the 4th day of the experiment, changes in the vascular-platelet link were established, indicating the exhaustion of the platelet potential, due to a decrease in blood clotting time, platelet aggregation ability, and blood clot retraction against the background of a probable increase in the number of blood platelets, which probably by now had time "get" to the bloodstream from the depot. Changes in the coagulation hemostasis system against the background of experimental fecal peritonitis indicate the development of hypercoagulation already on the first day, which is replaced by a hypocoagulation state, which indicates the exhaustion of the enzymatic systems of blood coagulation potential (as early as on the 4th day), which lasted until the end of the experiment.&#x0D; Conclusion. In the development of disorders of the hemostasis system in fecal peritonitis, the key role belongs to both the internal and external mechanisms of activation of the blood coagulation system.

HEMOGRAM INDICATORS ASSESSMENT FOR EARLY PREDICTING THE MIXED INFECTION OF THE ERYTHEMA MIGRANS FORM OF LYME BORRELIOSIS AND TICK-BORNE ENCEPHALITIS

Background. It is difficult to confirm the diagnosis of the mixed infection of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis at the onset of the disease because of delayed seroconversion.&#x0D; Purpose. Assessment of hemogram predictors to develop a model for early diagnosis of the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis.&#x0D; Materials and methods. The study involved 21 patients with the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis (Group 1) and 25 patients with the erythema form of borreliosis monoinfection (Group 2), who were hospitalized no later than the 7th day of the disease. The hemogram parameters, the leukocyte intoxication index and the body resistance index were analyzed. ROC analysis was used. The logistic regression model was developed using STATISTICA 12.0 modules.&#x0D; Results. The Group 1 patients compared with Group 2 demonstrated significant increases in the levels of the leukocyte intoxication index, and neutrophils, but decreases in the levels of the body resistance index, eosinophils, basophils and platelets. The leukocyte intoxication index was a “very good” predictor of the mixed infection, and the body resistance index, the number of eosinophils, basophils, neutrophils and platelets in peripheral blood had a “good” predictive values. The model included the body resistance index, the number of eosinophils and basophils.&#x0D; Conclusion. The developed model had a “very good” predictive value for the mixed infection early diagnosis of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis.

Green Tea extrat Effect on number of blood Criteria of lab rabbits Afflicted whith Entamoeba histolytica parasite

The study includes the effect of alcoholic extract of green tea on the Newzealand white male rabbits putrefied by Entamoeba histolytica parasite which causes Amoebic dysentery disease. The study dependes on several criteria including the examination of variations in complete blood picture (C.B.P) presented by the whole and differential account of white and red blood cells, pressed red cells volume (P.C.V), hemoglobin (Hb) rate, and blood platelets number. The study concludes that variations in blood component occur when parasites enter and after the treatment with green tea alcoholic extract; where significant decrease result at level (P&lt;0.01) in the total amount of white cells and granular cells and insignificant decrease in lymphatic cells, and single cells and red blood cells. hemoglobin rate also increased as well These results show the efficiency of green tea alcoholic extract in improving blood standards through variations occurring in blood proportion.

Hemostasis of cows in the first three months of pregnancy

Obtaining healthy young animals and high productivity from cows depends on the physiological and biochemical status of the animal organism. A significant role in this process belongs to the system of hemostasis, the formation of the feto-placental complex, the maximum supply of nutrients and oxygen to the fetus. Platelet hemostasis of cows undergoes significant changes during pregnancy. They testify that during the period of bearing the fetus, the state of vascular and platelet hemostasis of the animal acquires the corresponding regularities in the dynamics. It was established that the period of fetal nidation is accompanied by a decrease in the number of blood platelets in the blood of cows by 1.16 times, compared to the indicator of non-calving animals (p < 0.05. in the blood was found to be 1.17 – 1.16 times (p < 0.05) less than in control cows. The number of platelets in the blood of cows from the first trimester of pregnancy to the end of the third trimester decreases by 1.17 (p < 0 .05), 1.28 (p<0.05) and 1.33 times (p<0.01), and 1.27 times (p<0.01) during the entire calving period. In calving cows at the end in the first trimester of pregnancy, the prothrombin time was 1.21 times (p<0.05), shorter, compared to this indicator of cows of the control group. In pregnant cows, the prothrombin time of hemostasis decreased from the first trimester of pregnancy. On average, over the entire period of pregnancy in cows, the prothrombin time was 1.34 times less than in non-calving cows (p<0.01). it is also shown by the prothrombin index of hemostasis of cows. In cows, the prothrombin index consistently decreased during the first trimester (p<0.05). We found significant changes in the content of fibrinogen in the blood of cows. In pregnant animals, the content of fibrinogen in the blood gradually increased and at the end of the first trimester of pregnancy was more than at the beginning of pregnancy. Changes in the dynamics of indicators of prothrombin and thrombin time of hemostasis, the content of fibrinogen in the blood of cows during this gestation period affect the properties of blood. By the end of the first trimester of pregnancy, the viscosity of the cows' blood increased, and the coagulation rate of the blood of pregnant cows increased.

VASCULAR-PLATELET HEMOSTASIS OF INJURED PATIENTS: PROSPECTIVE OBSERVATIONAL STUDY.

The aim: We study vascular-platelet hemostasis peculiarities in patients with severe trauma. Materials and methods: We included 50 patients, who were divided into control (n=15) and study (n=35) groups. The control group included patients without traumatic injuries, study group - patients with severe trauma. The study group was divided into the I subgroup (patients received 1 g tranexamic acid IV at the prehospital stage), and the II subgroup (1 g tranexamic acid IV after hospital admission). Results: The main changes in the I subgroup started on the 3rd day, while in the II subgroup - on the 1st day. Patients of both subgroups on the 1st and 3rd days had a normal number of platelets in venous blood, however, on the 3rd day, there was a decreasing level of discocytes whereas the level of discoechinocytes, spherocytes, spheroechinocytes, and the sum of active forms of platelets were increased in comparison with the control group (p<0.05). Conclusions: The changes in vascular-platelet hemostasis in patients appeared in the I subgroup on the 3rd day, while in the II subgroup - on the 1st day. For the I subgroup was the decreasing level of discocytes, whereas the level of discoechinocytes, spherocytes, spheroechinocytes, and the sum of active forms of platelets were increased. For the II subgroup on the 1st day, there was an increasing sum of active forms of platelets, on the 3rd day - the level of discocytes was decreased, and levels of discoechinocytes, spherocytes, spheroechinocytes, and the sum of active forms of platelets were increased.

Study the antioxidant effect of coconut oil in rabbits treated with sodium nitrate

The present study was conducted to explore the effect of different concentrations of coconut oil as an antioxidant with rate of (1.5, 2 ,2.5ml/kg) of body weight given orally and measure Hematological parameters (white blood cells,PCV , Hb and platelets) and biochemical parameters (cholesterol and its different kinds (Total cholesterol, HDL, LDL, VLDL), triglycerides) and liver enzymes (Superoxide dismutase , Glutathione) in rabbits that demonstrated sodium nitrate as oxidative material use concentration (75 mg / kg), and compared to all results with control group . The results showed a decrease in triglycerides, LDL , SOD and GSH , increase HDL cholesterol levels and a raise in the number of white blood cells, platelets and Hb level this results belong to increase the number of free radicals as a result of oxidative stress caused by the use of sodium nitrate.

Testing the Impact of Vibration on Changes in the Number of Erythrocytes and Platelet Counts in Whole Blood

Blood or plasma is transferred from the unit car to the Blood Donor Unit using a conventional cooler box without any tools to maintain the effect of vibration which is very possible for a decrease in blood quality. Examination carried out to determine the quality of blood through routine hematological examination by counting the number of erythrocytes and platelets. The purpose of this study was to determine the impact of vibration on the number of red blood cells and thrombocytes. The research design was a pre and post test with control group design. The sample size for each group was nine tubes of blood. Data was processed by one way ANOVA and tukeys HSD test. Results There was a significant difference in the number of erythrocytes between the group that was vibrated at a frequency of 11 HZ compared to the group that was vibrated at a frequency of 0 HZ and 5 HZ with p values 0.00 and 0.01, respectively. There was a significant difference in the number of platelets between the groups that were vibrated with a frequency of 11 HZ compared to the groups that were vibrated with a frequency of 0 HZ and 5 HZ with a p value of 0.00. It was concluded that vibrations exceeding 10 Hz could decrease the number of erythrocytes and platelets in whole blood

Testing the Impact of Vibration on Changes in the Number of Erythrocytes and Platelet Counts in Whole Blood

Blood or plasma is transferred from the unit car to the Blood Donor Unit using a conventional cooler box without any tools to maintain the effect of vibration which is very possible for a decrease in blood quality. Examination carried out to determine the quality of blood through routine hematological examination by counting the number of erythrocytes and platelets. The purpose of this study was to determine the impact of vibration on the number of red blood cells and thrombocytes. The research design was a pre and post test with control group design. The sample size for each group was nine tubes of blood. Data was processed by one way ANOVA and tukeys HSD test. Results There was a significant difference in the number of erythrocytes between the group that was vibrated at a frequency of 11 HZ compared to the group that was vibrated at a frequency of 0 HZ and 5 HZ with p values 0.00 and 0.01, respectively. There was a significant difference in the number of platelets between the groups that were vibrated with a frequency of 11 HZ compared to the groups that were vibrated with a frequency of 0 HZ and 5 HZ with a p value of 0.00. It was concluded that vibrations exceeding 10 Hz could decrease the number of erythrocytes and platelets in whole blood