Number Of Pathogenic Bacteria Research Articles

Chemical Composition and Toxicological Evaluation of Landfill Leachate from Białystok, Poland

Leachates from landfills constitute a very complex environmental matrix with potentially toxic properties for both the environment and humans. Due to this fact, toxicological tests were carried out on landfill leachate (LL) obtained from the local landfill in Hryniewicze (Poland). The analyses included physicochemical studies of leachates and their impact on soil and plants, and studies conducted in bacterial models and human cell models. The results obtained indicate high contents of metals and organic matter, especially dangerous PAHs, in the tested leachate. This results in the influence of LL on changes in the content of assimilation pigments and oxidative stress observed in plants grown in soil fed with leachates. The effect of leachate on the growth of Sporosarcina pasteurii, Staphylococcus aureus, Lactobacillus rhamnosus, Saccharomyces boulardii and Candida albicans varied depending on the strain and LL dose. A particularly significant increase in proliferation after exposure to LL was noted for S. aureus. In studies conducted on human cancer cell lines representing three types of glioblastomas and one type of colorectal adenocarcinoma, a particularly significant increase in the viability of cells treated with LL was noted for the DLD-1 cell line. The results obtained, especially the stimulation of the growth of cancer cells and an increase in the number of pathogenic bacteria, indicate the potential toxic properties of the tested leachates. This is confirmed by the high level of oxidative stress in plants. The results indicate the need for continuous monitoring of waste landfills and leachates generated there.

The Effect of Adding Xylanase Enzyme to Diets of Broilers Containing De-Hulled Sunflower Meal as a Substitute for Soybean Meal on Growth, Gastrointestinal Mass, Biology, and Intestinal Morphology, Blood Serum Biochemical Traits

This study was conducted in the Poultry Research Hall of the Department of Animal Production/College of Agriculture and Forestry/University of Mosul, with the aim of studying the effect of adding xylanase enzyme to broiler rations containing de-hulled sunflower meal instead of soybean meal on digestive mass, biology and morphology of the intestines, and serum biochemicals Traits. It used 360 non-sexed broiler chicks (Ross-308), with an average hatching weight of 41.50 grams. the chicks were randomly distributed in a factorial experiment with two factors (4×2) to eight feeding treatments, with three replicates for each treatment and 15 chicks for each replicate, homogeneous in their weights. And for a period of 42 days of age. The results indicated that the substitution of de-hulled sunflower meal by 33% did not have a significant effect on live body weight, feed consumption, intestinal morphology and biology, and blood serum biochemistry compared with the control treatment. While it was observed that a significant deterioration (P≤0.05) occurred in all of these characteristics when the substitution level was increased to 66 and 100%, these two treatments also caused an increase in the relative weight of the gizzard and small intestine and in the number of pathogenic bacteria (E. coli) compared with the control treatment. The results of the statistical analysis of the interference case showed a significant decrease (p≤ 0.05) for the partial and total substitution treatments (66 and 100% Dh-SFM with or without xylanase enzyme) in the live body weight, with a deterioration in the morphological characteristics of the jejunum segment and an increase in the relative weight of the gizzard. And small intestine compared with the negative and positive control treatments.

Physicochemical and Biological Characterization of Opuntia ficus-indica (L.) Mill. Seed Oil Collected from The Bordj Bou Arreridj Region, Algerian Highlands

Background: The prickly pear is well adapted to arid and semi-arid areas. It occupies an important part in the human diet and it is also used as fodder for livestock. It is an interesting plant due to the environmental conditions in which it grows and its resistance to extreme climatic conditions. The objectives of our study were to determine the biological effects of oil extracted from prickly pear seeds (Opuntia ficus-indica L. Mill.). Methods: The organoleptic characteristics and the physicochemical parameters of this oil were done. The antibacterial and antifungal effects of the oil were tested in vitro against certain numbers of pathogenic bacteria and fungi by the aromatogram method. The antioxydante activity and the GC-MS analysis were proceeded also in this study. Result: The results show that the bacterial strains Klebsiella pneumoniae and Enterococcus faecalis are the most sensitive to the acid oil of Opuntia ficus-indica (L.) Mill. with an inhibition diameter varying between 23 mm and 25 mm, respectively. On the other hand, prickly pear seed oil exhibits an antifungal effect mainly against Candida albicans (25 mm). The oil extracted was strongest active (IC50 = 0.55 mg/ml). This indicates that our oil exhibited high reducing activity. 18 components were found in prickly pear seeds oil after analysis by GC-MS, which are presented mainly by phthalic acid (45.04%), monounsaturated fatty acid (18.56%), polyunsaturated fatty acid (16.69%) and other components (19.71%). The oil of Opuntia ficus-indica (L.) Mill. seeds showed good biological activity grace to its richness in components.

Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR.

Pectobacterium brasiliense (Pbr) has caused significant economic losses in major vegetable production areas in Northern China by causing bacterial soft rot in cash crops such as potatoes and cucumbers. This study aimed to establish a PMA-qPCR detection method for Pbr by screening specific and sensitive primers based on the glu gene and the conserved region of the 23S rRNA gene. Based on the optimized PMA pretreatment conditions, a standard curve was designed and constructed for PMA-qPCR detection (y = -3.391x + 36.28; R2 = 0.99). The amplification efficiency reached 97%, and the lowest detection limit of viable cells was approximately 2 × 102 CFU·mL-1. The feasibility of the PMA-qPCR method was confirmed through a manually simulated viable/dead cell assay under various concentrations. The analysis of potato tubers and cucumber seeds revealed that nine naturally collected seed samples contained a range from 102 to 104 CFU·g-1 viable Pbr bacteria. Furthermore, the system effectively identified changes in the number of pathogenic bacteria in cucumber and potato leaves affected by soft rot throughout the disease period. Overall, the detection and prevention of bacterial soft rot caused by Pbr is crucial.

Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions.

Recently, studies have highlighted the potential danger for soil organisms posed by film-derived microplastics (MPs). However, the majority of those does not accurately reflect the field conditions and the degree of MP contamination that can be found in actual settings. To fill the gap between laboratory and field scenarios, the polyethylene (PE) plastic film was made into PE-MPs and aged. Toxicity and molecular mechanisms of pristine PE-MPs (PMPs) and aged PE-MPs (AMPs) with the concentration at 500 mg/kg of dry weight were determined after 14 days of exposure by measuring the oxidative stress, osmoregulation pressure, gut microbiota, and metabolic responses in earthworms under environmentally relevant conditions. Our research showed that, when compared to PMPs (13.13 ± 1.99 items/g), AMPs accumulated more (16.19 ± 8.47 items/g), caused more severe tissue lesions, and caused a higher increase of cell membrane osmotic pressure in earthworms' intestines. Furthermore, the proportion of probiotic bacteria Lactobacillus johnsonii in the gut bacterial communities was 24.26%, 23.26%, and 12.96%, while the proportion of pathogenic bacteria of the phylum Verrucomicrobia was 2.28%, 4.79%, and 10.39% in the control and PMP- and AMP-exposed earthworms, indicating that the decrease in number of probiotic bacteria and the increase in number of pathogenic bacteria were more pronounced in the gut of AMP- rather than PMP-exposed earthworms. Metabolomic analysis showed that AMP exposure reduced earthworm energy metabolites. Consequently, the constant need for energy may result in protein catabolism, which raises levels of some amino acids, disturbs normal cell homeostasis, causes changes of cell membrane osmolarity, and destroys the cell structure. Our studies showed that aged MPs, with the same characteristics as those found in the environment, have greater toxicity than pristine MPs. The results of this study broaden our understanding of the toxicological effects of MPs on soil organisms under environmentally relevant conditions.

Effect of adding two levels of dried Molokhia (Corchorus Olitorius L.) leaves to the diet on some physiological characteristics and bacterial numbers in the intestines of broilers

This study has tackled the poultry field of (Abu Ghraib site) related to the animal production Department/ College of Agricultural Engineering Sciences/ University of Baghdad for 42 days (from 15 Oct 2021 to 26 Nov 2021). The study aims to investigate the effect of adding the powder of Corchorus olitorius dried leaves to the diet for different periods on the productive performance of broilers. Three hundred fifteen broiler chicks are used (Ross 308 breed) regardless of gender, of one day age and an initial weight of 40 gm. The chicks are divided into seven coefficients and three replicators for each coefficient; each replicator includes 15 chicks. The chicks starting from age 1-21 days are fed with a (starter) chicken feed consisting of 3006.5 kilo calories/ Kg. Feed metabolized energy and 22% raw protein. Then, the chicks are fed with a (finisher) chicken feed from 22-42 days of age. The finisher comprises 3171.11-kilo calories/ Kg. of feed metabolized energy and 20.54 raw protein. Biochemical tests reveal no effect of the coefficients of adding powder of Corchorus olitorius L. dried leaves on the averages of glucose and phosphorus in blood serum. An improvement (P˂0.05) of T4 coefficient covering albumin, globulin, cholesterol, and whole averages of protein is shown. As for glutathione, all adding coefficients show improvements (P˂0.05) compared to the control coefficient. Coefficient T7 (5.47mg) shows a more significant increase, whereas the lowest is (4.14mg/ml). The results indicated that treatments of adding dried Molokhia leaves powder led to a significant (P < 0.05) increase in the number of Lactobacillus beneficial bacteria compared to the control treatment. In contrast, a significant (P < 0.05) increase was observed in the number of pathogenic bacteria, E. coli, for the control treatment compared to addition treatments for all periods. Keywords: chicken feed, dried leaves, molokhia, physiological traits, lactobacillus.

Design of Nanohydroxyapatite/Pectin Composite from Opuntia Ficus-Indica Cladodes for the Management of Microbial Infections.

Hydroxyapatite (HAp) attracts interest as a biomaterial for use in bone substitution or allografts. In the current work, biomaterial nanocomposites based on HAp and pectin were synthesized by using the double decomposition method, which involved using pectin extracted from fresh cladodes of the prickly pear, Opuntia ficus-indica. The crystallinity, purity, and several analytical techniques like Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy were used to understand the surface’s shape. The results revealed that the produced HAp/pectin nanoparticles are pure, spherical, and amorphous. The spectroscopic data indicated a substantial interaction between HAp and pectin, specifically between Ca (II) and pectin hydroxyl and carboxyl groups. The presence of pectin showed a noticeable influence on the prepared nanocomposite texture and porosity. We further assess the antibacterial and antifungal activity of the developed nanocomposite against a number of pathogenic bacteria and fungi, evaluated by the well diffusion method. In the absence of pectin, the XRD analysis revealed that the HAp nanoparticles had 10.93% crystallinity. When the pectin concentration reached 10 wt.%, it was reduced to approximately 7.29%. All synthesized nanocomposites demonstrated strong antimicrobial activity against both Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in addition to various fungi (e.g., Aspergillus fumigatus, Penicillium funiculosum, and Trichoderma viride). This study endorses the HAp/Pectin nanocomposite as an efficient antimicrobial material for biomedical advanced applications.

Sulfur-bridged pyrazole-pyrimidine hybrids as promising anti-tubercular agents: Synthesis, biological evaluation, molecular docking, and toxicity prediction

• Sulfur-bridged pyrazole-pyrimidine hybrids were synthesized. • In vitro anti-tubercular activity of some hybrids was compared to that of ethambutol. • The hybrids might target enzymes from arabinogalactan biosynthesis. • Satisfactory toxicity profiles were in silico predicted for the active hybrids. A series of 4,6-dimethyl-2-[(3,5-dimethyl-1-R-pyrazol-4-yl)sulfanyl]pyrimidines (3a-l) with good drug-likeness profiles were synthesized via cyclization of the key intermediate, 3-[(4,6-dimethylpyrimidin-2-yl)sulfanyl]-4-hydroxypent-3-en-2-one (4), with various hydrazines. Heterocycles 3a-l were evaluated for their biological activities against a number of pathogenic bacteria and fungi. Two of these compounds bearing 4-methylphenyl (3b) and 4-bromophenyl (3c) substituents at the pyrazole ring possessed a significant anti-tubercular effect with minimal inhibitory concentration (MIC) values of 3.13 and 6.25 μg/mL, respectively. These values were comparable to the MIC for ethambutol (5 μg/mL) used as the reference drug. As a molecular docking study revealed, heterocycles 3b and 3c might target some enzymes involved in the biosynthesis of arabinogalactan, an important component of the mycobacterial cell wall. Along with satisfactory in silico predicted toxicity data, the obtained results indicate that further search for potential anti-tubercular drugs among heterocycles 3a-l may be promising. .

Enhancement of growth, survival, immunity and disease resistance in Litopenaeus vannamei, by the probiotic, Lactobacillus plantarum Ep-M17

Green ecological prevention and control technology is a hot spot for aquatic disease research in recent years, and lactic acid bacteria is an important type of probiotic widely used in aquaculture. In this study, a strain of Lactobacillus plantarum Ep-M17 was isolated from the intestine of healthy grouper, which showed good antibacterial activity in vitro. To investigate the application prospects of Ep-M17 as a probiotic, we added it to the diet and fed Litopenaeus vannamei, and then detected its influence on the growth performance, survival rate, disease resistance, intestinal tissue structure, gene transcription, and the flora in the gut of shrimp. The results showed that feeding Ep-M17 increased the specific growth rate, reduced the feed conversion rate, improved the survival rate, and achieved a 76.9% relative protection rate after Vibrio parahaemolyticus E1 infection in shrimp. Histological examination displayed that Ep-M17-fed shrimp had a thick intestinal villi layer, which enhanced the protection against pathogen damage. It was also found that Ep-M17 significantly increased the activity levels of immune and digestion-related enzymes SOD, CAT, TRY, AKP, LIP, and AMS in the gut of shrimp, especially after V. parahaemolyticus E1 infection, these enzymes increased significantly higher than that of control. Transcriptome analysis revealed that Ep-M17 activated significantly differential expression of genes in immune, nutritional, metabolic, and Signal Transduction-related pathways in the gut of shrimp. In addition, Ep-M17 enriched the bacterial diversity of the shrimp gut, with a significant increase in many low-abundance bacterial species, a significant decrease in the number of pathogenic bacteria like Vibrio, and a significant increase in the number of beneficial bacteria. The above results evaluated that Ep-M17 as a potential probiotic can promote the growth and improve the disease resistance of shrimp by regulating the nutritional immune response and flora of the intestine.

Microbial assessment of water, sediment and tilapia fish of Lake Nasser, Egypt

ABSTRACT Lake Nasser is the main source of fresh water in Egypt. This study concerns microbial assessment of water, sediments and fish (Oreochromis niloticus) collected from 5 inlets or khors of Lake Nasser (Rahma, Garf Hussein, Korosko, Toushka and Abu Simbel). The results indicate that the water and sediment samples collected from the southern part of Lake Nasser (Toushka) recorded a higher microbial load than the northern part. The highest number of pathogenic bacteria was also recorded in the southern part of Lake Nasser (Toushka and Abu Simbel). Also a higher bacterial load in/on fish (O. niloticus) was recorded at Rahma and Toushka sites. In general, the results of this study show that Lake Nasser is exposed to pollution, especially in its southern part. Therefore, regular monitoring and assessment are necessary to maintain the quality of Lake Nasser.

Influence of green tea powder on the performance, nutrient utilisation, caecal microbiota profile and meat quality of broiler chickens

This study assessed the influence of green tea powder diet supplementation on performance, nutrient utilisation, caecal microbiota profile and meat quality in broiler chickens. A total of 144, one-day-old broiler (Ross 308) chicks were allocated to 18 cages (eight broilers/cage) which were randomly assigned to one of three dietary treatments: a wheat-based basal diet or supplemented with one of two types of green tea; normal (N-GT) or selenium-rich (Se-GT) tea, at an inclusion rate of 1%. Bird performance, nitrogen-corrected apparent metabolisable energy (AMEn), and total tract digestibility of fat and starch were measured on d 7, 21 and 35. Effects on microbiota profile on d 7, 21 and 35 were determined in a cohort study. Green tea supplementation reduced (P<0.05) the weight gain and feed intake but improved (P<0.05) feed efficiency. Supplementation with N-GT increased the AMEn on d 7 and 21, and with Se-GT on d 7 (P<0.05). AMEn increased with age for all treatments. Fat digestibility increased (P<0.05) in birds fed N-GT on d 21 and Se-GT on d 7 and 21. Starch digestibility increased (P<0.05) on d 21 with N-GT supplementation and on d 7 with Se-GT supplementation. Carcass and breast meat yields were unaffected (P<0.05) by the dietary treatments. The abdominal fat pad decreased (P<0.05) in the N-GT diet and numerically in the Se- GT diet. Drip loss was less in meat from birds (P<0.05) fed both green tea treatments. Cooking loss was reduced in the Se-GT treatment (P<0.05). Dietary inclusion of green tea powder positively influenced microbiota profile, with increased (P<0.05) numbers of beneficial bacteria (Lactobacillus spp. and Bifidobacterium spp.) and reduced (P<0.05) numbers of pathogenic bacteria (Clostridium spp. and Bacteroides spp.). Differences were observed between the two green tea types, with Se-GT being more beneficial than N-GT in the positive modulation of intestinal microbiota.

Biosensors, modern technology for the detection of cancer-associated bacteria.

Cancer is undoubtedly one of the major human challenges worldwide. A number of pathogenic bacteria are deemed to be potentially associated with the disease. Accordingly, accurate and specific identification of cancer-associated bacteria can play an important role in cancer control and prevention. A variety of conventional methods such as culture, serology, and molecular-based methods as well as PCR and real-time PCR have been adopted to identify bacteria. However, supply costs, machinery fees, training expenses, consuming time, and the need for advanced equipment are the main problems with the old methods. As a result, advanced and modern techniques are being developed to overcome the disadvantages of conventional methods. Biosensor technology is one of the innovative methods that has been the focus of researchers due to its numerous advantages. The main purpose of this study is to provide an overview of the latest developed biosensors for recognizing the paramount cancer-associated bacteria.

Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics.

Amebiasis is an intestinal disease transmitted by the protist parasite, Entamoeba histolytica. Lactobacillus acidophilus is a common inhabitant of healthy human gut and a probiotic that has antimicrobial properties against a number of pathogenic bacteria, fungi, and parasites. The aim of this study was to investigate the amebicide activity of L. acidophilus and its mechanisms. For this purpose, E. histolytica and L. acidophilus were co-incubated and the parasite’s viability was determined by eosin dye exclusion. The level of ozidized proteins (OXs) in the parasite was determined by resin-assisted capture RAC (OX–RAC). Incubation with L. acidophilus for two hours reduced the viability of E. histolytica trophozoites by 50%. As a result of the interaction with catalase, an enzyme that degrades hydrogen peroxide (H2O2) to water and oxygen, this amebicide activity is lost, indicating that it is mediated by H2O2 produced by L. acidophilus. Redox proteomics shows that L. acidophilus triggers the oxidation of many essential amebic enzymes such as pyruvate: ferredoxin oxidoreductase, the lectin Gal/GalNAc, and cysteine proteases (CPs). Further, trophozoites of E. histolytica incubated with L. acidophilus show reduced binding to mammalian cells. These results support L. acidophilus as a prophylactic candidate against amebiasis.

Effect of adaptogen lithium ascorbate on the microbiota of rumen in young ewes

Health and productivity of ruminants directly depend on the state of rumen and digestion in intestinal tract. Normal microflora protects the body from pathogenic microbes, stimulates the immune system, participates in metabolic reactions and plays an important role in energy metabolism. Disturbance of species composition of microflora in rumen under effect of various factors leads to a state of dysbiosis, disorders of products digestibility, changes in enzymatic processes and other disorders. Biologically active substances of an adaptogenic nature can show immunomodulatory and antimicrobial activity. Adaptogen lithium ascorbate has stress-protective, neurotrophic, neuroprotective, antioxidant, immunomodulatory properties. In this paper, using molecular genetic methods, the effect of lithium ascorbate on composition of rumen microbiota of sheep was studied for the first time. Composition of microbiota was determined by NGS sequencing method. In total, 37 phylums, 76 classes, 98 orders, 225 families and 894 species of microorganisms were identified. It has been determined that introduction of lithium ascorbate at a dose of 10 mg/kg of body weight into the main diet of sheep contributed to increase in proportion of cellulolytic bacteria from 73.6±1.6% to 75.4±0.9% and over 40% decrease in total number of infusoria in rumen content. Cellulolytic activity of rumen fluid in sheep of the experimental group increased by 38% compared to the animals of the control group. The number of pathogenic bacteria decreased from 2.7% in sheep of the control group to 1.6% in animals of the experimental group, while the content of beneficial bacilli increased from 0.3% to 0.5%, respectively. Content of undesirable and conditionally pathogenic microflora decreased in the sheep of experimental group. The obtained data confirm positive effect of adaptogen lithium ascorbate, introduced into diet at a dosage of 10 mg/kg of body weight, on qualitative and quantitative composition of microbiota in sheep rumen. Therefore, lithium ascorbate, a broad-spectrum adaptogen, can be recommended as a feed additive to the main diet of sheep to improve rumen digestion and increase productivity.

Effect of Xiaoning liquid on gut microbiota in asthmatic mice by 16S rDNA high-throughput sequencing

To investigate the effects of Xiaoning liquid on gut microbiota in mouse during asthma. A total of 60 mice were randomly and averagely assigned to healthy control group, control group, budesonide group, and Xiaoning liquid group. The later three groups were used to establish an Ovalbumin (OVA) asthma model. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification. Analyzing the structure of gut microbiota with OTU analysis, Shannon-Wiener, PCA, PCOA, etc. 16S rDNA high- throughput sequencing. The abundance and diversity of the gut microbiota in asthmatic mice increased, most obviously in the control group. The Bacteroidetes and Firmicutes levels increased in all asthmatic mice. The level of Bacteroides increased most obviously, making Bacteroides a useful marker of gut microbiota changes in asthmatic mice. The levels of Proteobacterium, Deferribacteraceae and Mucispirillum dropped significantly in the Xiaoning liquid group. Xiaoning liquid can reduce the species and numbers of pathogenic bacteria and restored the intestinal microecology of asthmatic mice. Xiaoning liquid has a positive effect on the function of gut microbiota.

Water quality assessment based on physico-chemical and biological analysis of Khari River

Throughout the world, the pollutants of various categories enter the water resources, including household, industrial and agricultural waste resulting in the reduction of water quality. As a result, the total hardness, alkalinity, and organic content of the water increase with the simultaneous increase in the number of pathogenic bacteria. As a result, water becomes unfit for common, industrial, and agricultural use. One way to determine water quality is based on an assessment through Physico-chemical and biological analysis. The purpose of this study was to determine the pH, dissolved oxygen (DO), nitrates, total dissolved solutes (TDS), and the density of coliform bacteria in the Khari River, Agra (India). This research was carried out using sampling at five stations with five points at each station in three seasons of the year. Analysis of the data included the quantitative analysis through the most probable number (MPN) method. The results showed that the average density of total coliform bacteria was out of the range for potable water. The total presence of coliform bacteria is dependent upon the presence of nitrates and total organic matter in the Khari River. The average total density of coliform bacteria and the water quality index obtained at each station does not meet the quality standard indicating the pollution status in Khari River is categorized as mild to moderate polluted.

Comparative Study of the Gut Microbiota Among Four Different Marine Mammals in an Aquarium.

Despite an increasing appreciation in the importance of host–microbe interactions in ecological and evolutionary processes, information on the gut microbial communities of some marine mammals is still lacking. Moreover, whether diet, environment, or host phylogeny has the greatest impact on microbial community structure is still unknown. To fill part of this knowledge gap, we exploited a natural experiment provided by an aquarium with belugas (Delphinapterus leucas) affiliated with family Monodontidae, Pacific white-sided dolphins (Lagenorhynchus obliquidens) and common bottlenose dolphin (Tursiops truncatus) affiliated with family Delphinidae, and Cape fur seals (Arctocephalus pusillus pusillus) affiliated with family Otariidae. Results show significant differences in microbial community composition of whales, dolphins, and fur seals and indicate that host phylogeny (family level) plays the most important role in shaping the microbial communities, rather than food and environment. In general, the gut microbial communities of dolphins had significantly lower diversity compared to that of whales and fur seals. Overall, the gut microbial communities were mainly composed of Firmicutes and Gammaproteobacteria, together with some from Bacteroidetes, Fusobacteria, and Epsilonbacteraeota. However, specific bacterial lineages were differentially distributed among the marine mammal groups. For instance, Lachnospiraceae, Ruminococcaceae, and Peptostreptococcaceae were the dominant bacterial lineages in the gut of belugas, while for Cape fur seals, Moraxellaceae and Bacteroidaceae were the main bacterial lineages. Moreover, gut microbial communities in both Pacific white-sided dolphins and common bottlenose dolphins were dominated by a number of pathogenic bacteria, including Clostridium perfringens, Vibrio fluvialis, and Morganella morganii, reflecting the poor health condition of these animals. Although there is a growing recognition of the role microorganisms play in the gut of marine mammals, current knowledge about these microbial communities is still severely lacking. Large-scale research studies should be undertaken to reveal the roles played by the gut microbiota of different marine mammal species.

ISOLATION AND GENETIC DETECTION OF MORAXELLA BOVIS FROM BOVINE KERATOCONJUNCTIVITIS IN BASRAH CITY

This study was aimed to determine the most sensitive isolation procedures and evaluate the genetic diversity of Moraxella bovis because there are large number of pathogenic bacteria and several other infectious agents such as virus and Mycoplasma have been isolated from the eyes infected with infectious bovine keratoconjunctivitis (IBK). This study included examination of (40) eye swabs, from cows from different ages and regions in Basrah city showed clinical signs of an ocular infection. The isolated bacteria that obtained in pure cultures were non-motile, catalase and oxidase positive, and a clear zone of β-hemolytic colonies were produced on blood agar. According to the growth characteristics, morphology, staining and biochemical tests, the isolated bacteria were identified initially as Moraxella spp., the genetic diversity among Moraxella spp. was assessed by 16S rRNA and sequencing as M. bovis. The study has also indicated that the isolates of M. bovis were resistant to tetracycline, chloramphenicol and erythromycin, However, they were sensitive to penicillin and gentamicin and has an intermediate sensitivity to streptomycin. This study concluded that PCR techniques and sequencing were verified to be most accurate tools to indicate the genetic diversity between Moraxella spp. in bovine keratoconjunctivitis.

Study on the implementation of different biofloc sedimentable solids in improving the water quality and survival rate of mud crab, Scylla paramamosain larvae culture

Microbial communities in biofloc technology (BFT) are responsible in minimalize water exchange and producing microbial proteins that are beneficial as a supplementals diet for mud crab, Scylla paramamosain larvae culture. Therefore, a study was conducted to determine the effect of bacterial communities in different sedimentable solids in order to improve the water quality and survival rate of S. paramamosain larvae culture. The results successfully identified that the number of pathogenic bacteria, Vibrio spp. decreased when the heterotrophic bacteria thrived in treatment tank with 4 ml/L sedimentable solids. The group of heterotrophic bacteria were able to adapt in developing their population while effectively uptake the ammonium and nitrite components. The ammonium and nitrite level in treatment tank depleting at an early stage of the experiment, and constantly low until the end of experiments (ammonium <0.20 mg/L, nitrite <0.10 mg/L). The survival rates of S. paramamosain larvae in both control and treatment tanks were identified <2.0%, however, sedimentable solids of 2 ml/L was identified suitable to be applied in crab larvae culture as it can help to reach megalopa stages within 17 days of culture period. Overall, it can be concluded that bacteria communities in biofloc are able to regulate nutrients load and maintaining water quality and also help in increased survival rate and development performance in the low sedimentable solids of biofloc culture treatment. Therefore, sedimentable solids of 2 ml/L is suggested to be potentially applied in S. paramamosain larvae cultures from this research finding.

Microbial pattern of urinary catheterization infection at Wahidin Sudirohusodo Hospital

Infectious disease still remains a serious problem in Indonesia and worldwide, it leads to use of uncontrollable anti-microbial treatment with cause to drug resistance. With unsupported condition for using a sensitivity test prior to antimicrobial treatment, a common guide for choosing an antimicrobial agent for infection of specific specimen is needed. The aims of this research is to determine the pattern of bacteria, more specific specimen is urine. This research was an observational Experimental consecutive sampling study, sample for this research were taken from inpatient room of Wahidin Sudirohusodo Hospital of Makassar during 2019-2020. The largest number of pathogenic bacteria that potentially becoming infection agent in inpatient room of Wahidin Sudirohusodo Makassar was E.coli, P.Aureginosa and Acinetobacter. Most of antimicrobial found in range of 61-70 years Old, and there was no significant relationship between sex and infections Sickness