Number Of Fruiting Bodies Research Articles

Microclimate and the spatio-temporal dynamics of natural production of two ectomycorrhizal fungi from the Guineo-Sudanian ecozone in Benin

The study aims to determine the relation between the micro-climate and the natural production of Amanita subviscosa, and Lactifluus gymnocarpoides, in the Guinea-Sudanian ecozone. Nine permanent plots of 50 ×50 m were installed in three different vegetations of the “Ouémé Supérieur” forest reserve in central Benin. The mycological surveys were carried out during three (3) mycological seasons, each from June to October at a frequency of two visits per week and per plot. The number of fruiting bodies and the fresh biomass were recorded. Microclimatic parameters (soil water content, air temperature, soil temperature, relative humidity and precipitation) were measured using a rain gauge and a HOBO micro station data logger installed at the middle of each plot. We tested several Generalized Linear Mixed Effects Models (GLMM) using the lme4 package to obtain the effect of recorded microclimate factor on the number of fruit bodies of each species and the Linear Mixed Effects Model (LMM) using the package nlme for fresh biomass. The study showed that soil water content is the main driver of fungal fruiting phenology and increased biomass production. The fruiting of A. subviscosa is positively affected by air temperature and soil water content (p = 0.0004) but negatively affected by relative air humidity. The fructification of L. gymnocarpoides is only positively affected by the air temperature and negatively affected by relative air humidity and soil water content. Soil water content significantly affects the fresh biomass of A. subviscosa (p≈0) while the fresh biomass of L. gymnocarpoides is significantly affected by soil water content and relative air humidity (p≈0). All species for their natural production have specific response at microclimate parameters variation.

Effect of Neem Leaf Powder Supplementation in Casing Mixture on the Growth and Yield Parameters of white Button Mushroom [Agaricus bisporus (Lange) Imbach

Green mould caused by Trichoderma harzianum is a severe threat of white button mushroom [Agaricus bisporus (Lange) Imbach] cultivation causing heavy crop losses. The present experiment was conducted to manage the green mould fungus Trichoderma harzianum using neem leaf extracts. In vitro, results showed maximum mycelial growth inhibition of 64.59 % in T. harzianum using neem leaf extract at 4.5% concentration. Neem leaf extracts at 4.5% concentration showed maximum efficacy against pathogens. Further, an In vivo experiment was conducted to evaluate neem leaf powder at different concentrations @0.5%, 1.5%, 2.5%, 3.5% and 4.5% supplemented in the casing mixture combination viz., Farm yard manure (FYM) + Cocopeat + Sawdust (1:1:1) to monitor the growth and yield aspects of white button mushroom. Among all treatments used in the study, the results revealed that minimum average time taken for completion of spawn run (15.14 days) and pinhead initiation (18.28 days) as well as maximum number of fruiting bodies (14.71), fruiting bodies weight (22.54g), stalk length (2.81 cm) stalk diameter (1.85 cm) and pileus diameter (5.64 cm), and were recorded in T2 [FYM + Cocopeat + Sawdust (1:1:1) + neem leaf powder @ 1.5%]. Results have shown that T2 [FYM + Cocopeat + Sawdust (1:1:1) + neem leaf powder @ 1.5%] had the highest yield (470.09 g) and biological efficiency (27.11%).

Enhancing oyster mushroom cultivation by chickpea and wheat straw substrate for sustainable agriculture

This study aimed to explore the potential utilization of agro-lignocellulosic waste materials for oyster mushroom cultivation, addressing the challenge of managing waste associated with these materials. Various combinations of Chickpea Straw (CS) and Wheat Straw (WS) at different ratios-100% CS, 75% CS + 25% WS, 50% CS + 50% WS, 25% CS + 75% WS, and 100% WS-were investigated as substrates for mushroom cultivation. The experiment followed a ccompletely rrandomised design with four replications, monitoring developmental phases, yield, and biological efficiency (BE). Results indicated that using 100% WS as a substrate resulted in the fastest mycelium growth (spawn run), completing in an average of 14.50 days, 17.20 days from spawning to pinhead formation, and 20.60 days to first harvest, with the highest number of fruiting bodies produced. Chickpea straw contributed to the highest stipe width (1.52 cm), while WS 100% had the highest average yield (997.28 g) and BE (99.73%). A mixture of 75% CS and 25% WS showed promising results in terms of fruiting bodies, yield, and BE after 100% WS. Consequently, the study recommends the use of these substrates to optimize Pleurotus ostreatus cultivation yield.

Exploring agricultural waste substrate for enhancement of <i>Pleurotus ostreatus</i> cultivation

The proximate composition of the organic waste substrates used in cultivating Pleurotus ostreatus was analyzed, and the results revealed significant variations in various nutritional components among the substrates. Palm Chaff had the highest ash content (7.14%), followed by Oil Palm Empty Bunch (5.08%), Yam Peel (4.29%), and Saw Dust (1.36%). This indicated that Palm Chaff was the richest in minerals, while sawdust had the lowest mineral content. In addition, Yam Peel had the highest moisture content (8.46%), followed by Palm Chaff (5.87%), Oil Palm Empty Bunch (4.62%), and Saw Dust (2.83%). This suggested that Saw Dust was the driest substrate. Furthermore, Oil Palm Empty Bunch had the highest crude lipid content (1.94%), followed by Palm Chaff (1.38%), Yam Peel (1.13%), and Saw Dust (0.04%). Moreover, Oil Palm Empty Bunch had the highest crude protein content (2.56%), followed by Yam Peel (2.1%) and Palm Chaff (2.02%). Saw Dust had the lowest crude protein content (1.74%). Besides, Oil Palm Empty Bunch had the highest crude fiber content (48.27%), followed by Palm Chaff (36.94%), Saw Dust (26.17%), and Yam Peel (14.36%). In addition, Saw Dust had the highest carbohydrate content (67.86%), followed by Yam Peel (69.64%) and Palm Chaff (46.65%). Oil Palm Empty Bunch had the lowest carbohydrate content (37.58%). These findings offered valuable insights into the suitability of each substrate for cultivating Pleurotus ostreatus Additionally, it enabled growers to make informed decisions about substrate selection based on the specific nutritional needs and growth requirements of the mushrooms. The impact of various organic waste substrates on the growth dynamics of Pleurotus ostreatus (oyster mushroom), revealed significant variations in several key parameters. In primordial Formation, the number of early-stage fruiting bodies formed varied between 12 and 36 across different substrate formulations. The highest primordial formation was observed in treatment A + B + C + D (36), while treatments involving individual substrates or their combinations had the lowest primordial formation (12). Furthermore, in Fruiting Body Production, the number of mature mushrooms produced varied across treatments. Treatment A + C resulted in the highest number of fruiting bodies (26), while treatments involving combinations of substrates (B + C + D, A + B + C, A + B + C + D, C + D) had the lowest number of fruiting bodies (3). Overall, the findings highlight the influence of specific substrate combinations on various aspects of mushroom growth dynamics. However, the choice of growth substrates did not significantly impact the overall weight and biological efficiency of Pleurotus ostreatus in this study. These results provide valuable insights for optimizing mushroom cultivation practices.

Pertumbuhan dan hasil jamur merang (volvariella volvacea) pada berbagai media tanam limbah pertanian

Straw mushrooms are a food ingredient that is much sought after by the public because they have a delicious taste and are beneficial for health with a complete nutritional co mposition consisting of carbohydrates (8.7%); protein (26.49%); fat (0.67%); calcium (0.75%); phosphorus (30%); potassium (44.2%) vitamins and minerals. For the growth of straw mushrooms, they need nutrients which can be fulfilled by the planting medium which can come from agricultural waste. This research aims to determine the growth and yield of straw mushrooms on various types of agricultural waste growing media. This research was designed using a Completely Randomized Design (CRD) with 4 treatment levels, namely: P0 = 50 kg rice straw, P1 = 50 kg reeds, P2 = 50 kg corn cobs, P3 = 50 kg sugarcane bagasse. The research was repeated 4 times to obtain 16 treatments. The results of the study showed that the effect of significant differences in treatment was only found in the observation parameter of the number of fruiting bodies of straw mushrooms, while the other parameters did not show significant differences. The results of the observations showed that the planting media treatment with rice straw gave the best results with a fruit body number of 63.5 fruit bodies, a fruit body diameter of 3.05 cm, a fruit body height of 4.42 cm and a fruit body wet weight of 898 gr. The time for pinheads to appear on rice straw planting media is faster than other planting media

Performance of Waste Paper as a Substrate for Oyster Mushroom (Pleurotus ostreatus L.) Cultivation

Purpose: Cultivation of Pleurotus spp. (Oyster mushroom) is economically feasible, exploiting its great capacity to degrade cellulose, lignin and hemicelluloses present in organic wastes. Waste paper is a very common pollutant thrown as garbage with no recycling plan in the environment. In view of the growing importance of food security and environmental sanitation this study was conducted to evaluate the performance of waste paper as a substrate for cultivation of Oyster mushroom (Pleurotus ostreatus L.), supplemented with sawdust and wheat bran. Methods: Pure culture of Pleurotus ostreatus was propagated using sterilized wheat grain as substrate for spawn preparation. The collected waste paper along with the saw dust and wheat bran was mixed as per the design [three different substrate mixtures/treatments (T-1, T-2 and T-3) were prepared], composted for a week, filled in polyethene bags and sterilized. Substrate bags were inoculated with Oyster spawn and incubated in a cultivation room with controlled conditions (light, temperature, humidity and ventilation). Data for growth parameters (number of days for primordia growth, number of fruiting bodies per bag, crop yield, biological efficiency and contamination rate) were recoded for each bag of each treatment, compared against the control group (records of evaluated parameters from the mushroom extension unit) and were analyzed statistically using two-factor Analysis of Variance (ANOVA) with replication technique. Results: Treatment-3 (waste paper supplied with saw dust and wheat bran) showed high performance in terms of mean crop yield (150.58 ± 1.2g) and BE (43.1 ± 1.1%); while Treatment-1(waste paper alone without any supplement) scores lower BE (17.3±2.1%). Based on the Analysis of Variance (ANOVA) this study shows that all the growth parameters were found significantly different at p-value (p < 0.01) considering each growth parameter among the treatments. Conclusion: Therefore, waste paper along with required percentage of sawdust and wheat bran can be used as alternative substrate for cultivation of this prized mushroom reducing substrate expenses, adding a new food recipe to nutritional cuisine and reducing waste paper pollution on the environment on the other hand.

The Effect of Adding Tea Dregs on The Growth of Brown Oyster Fungus (Pleurotus cystidiosus) on Sawdust Planting Media

The aim of the research was to determine the effect of adding tea dregs on the growth and the effective composition of tea dregs on the growth of brown oyster mushrooms (Pleurotus cystidiosus) on sawdust planting media. The type of research is quantitative with an experimental method using a Non-Factoral Completely Randomized Design (CRD) with 5 treatments and 4 replications. Data analysis in the study used parametric tests, namely the Anova test and LSD test as well as non-parametric tests, namely the Kruskal Wallis test and the Mann-Whitney test with SPSS. The results showed a significant effect on mycelium growth (Sig 0.014), number of fruit bodies (Sig 0.016), fruit stalk length (Sig 0.017), wet weight of harvest (Sig 0.000) and a significant effect on the age at which fruit bodies emerged (Sig 0.045) and had no significant on the diameter of the fruit cap (Sig 0.088) of brown oyster mushroom (Pleurotus cystidiosus). The best treatment for each observation parameter was mycelium growth in treatment P4 (19.3938 cm), age of emergence fruit bodies in treatment P0 (34.5 days), number of fruit bodies in treatment P4 (5.375), diameter of fruit cap in treatment P0 (12.3 cm), fruit stalk length in treatment P4 (10 cm), and wet harvest weight in treatment P4 (91 grams). The most effective treatment for the growth of brown oyster mushrooms ((Pleurotus cystidiosus) is P4 treatment with 25% (375 grams of Tea Dregs + 75% (1,125 grams of Swadust).

THE EFFECT OF USING SAGU DRUGS (Metroxylon sagu) AND DRIED BANANA LEAVES (Musa paradisiaca) ON THE GROWTH OF WHITE OYSTER MUSHROOM (Pleurotus ostreatus)

Oyster mushrooms (Pleurotus ostreatus) are wood fungi that grow sideways on rotting wood stems. Mushrooms take food that has been made by other dead organisms (saprophyte), because they do not have chlorophyll, all types of saprophyte, especially those that grow on wood, can be easily cultivated. White oyster mushroom cultivation is easy to do because it does not require expensive maintenance costs and does not require spacious land. The aim of this research was to test the effect of using sago dregs and dried banana leaves on the growth of white oyster mushrooms. The results of this research indicate that there are differences in the growth of white oyster mushrooms based on different concentrations of growing media. The average mycelium growth, stem height, pileus diameter, number of fruit bodies, wet weight and dry weight of the best white oyster mushrooms were found in media with a concentration of 100% sawdust, while pind heads appeared in a concentration of 100% sago dregs

Bioconversion of pineapple wastes for production of Pleurotus pulmonarius (gray oyster mushroom) and Pleurotus ostreatus (white oyster mushroom)

AbstractMushroom cultivation is currently being widely ventured by farmers in Malaysia due to its high profits in a short time and low production cost. Mushrooms can be grown on various substrates such as rice husk and stalks, coconut fiber, and sawdust. This study was performed to find out the ability of using pineapple leaves waste as a substrate for the cultivation of Pleurotus pulmonarius (gray oyster mushroom) and Pleurotus ostreatus (white oyster mushroom) and compare the results obtained with mushrooms grown using traditional substrates (sawdust). The mushroom bags were prepared using a dry and wet medium of pineapple leaves waste. The results revealed that 60% of dry and wet pineapple leaves waste produced the highest output of P. pulmonarius and P. ostreatus in comparison to other percentages. The growing of P. pulmonarius on dry pineapple leaves waste substrate revealed the maximum average weight and number of fruiting bodies while P. ostreatus showed the best growth performance in wet pineapple leaves waste substrate. The nutritional content of 60% dry and wet pineapple leaves waste for both P. pulmonarius and P. ostreatus shown superior value in percentages of moisture, protein, fat, and carbohydrate compared to control. In the meantime, the mineral analysis revealed that 60% of the dry and wet pineapple leaves waste had greater levels of Cu, P, and Pb than the control. These findings suggested that the pineapple leaves waste can become a great alternative substrate for both P. pulmonarius and P. ostreatus cultivation for better economic and environmental benefits.

Araucaria angustifolia seed coat waste reduction through its utilization in substrate diversification for Pleurotus djamor production

Mushrooms of the genus Pleurotus are the second most cultivated and consumed variety worldwide due to their nutritional profile and the efficient use of agricultural and forestry residues as substrates. In order to explore the efficiency of seed coat lignocellulose from Araucaria angustifolia (pine nut) seeds (PSC) as a substitute substrate (20 %, 40 %, 60 % or 80 %) of Eucalyptus sawdust for the production of Pleurotus djamor (PD) was estimated the number of fruiting bodies (NFB), biological yield (BY), biological efficiency (BE), and nutritional and mineral composition. The PSC was separated from the almond seed, dried, ground and used as an alternative substrate (0–80 %) to Eucalyptus sawdust (400 g), which was mixed with wheat bran (97 g) and CaCO3 (3 g). Due to a higher Carbon/Nitrogen ratio of a PSC substrate, it was better compatibility for a fungal enzymology of PD mushroom than E. urograndis sawdust substrate. The substrate enriched with 40 % PSC exhibited elevated ash content (minerals) and reduced moisture levels, higher carbon/nitrogen ratio, optimal conditions for NFB, and increased BY and BE. PD showed significant levels of dietary fiber and essential elements, including Cu, Fe, P, Mn, Mg, and Zn, with minimal fat content. Araucaria angustifolia waste reduction through its utilization in substrate diversification for PD production brings about favorable environmental and societal outcomes, improving both the mushroom yield and their nutritional content. This approach serves as an environmentally sustainable solution for pinhão seed coat waste and should be encouraged and extended for broader use.

ЗНАХІДКИ ГРИБІВ ЧЕРВОНОЇ КНИГИ УКРАЇНИ НА ТЕРИТОРІЇ НАЦІОНАЛЬНОГО ПРИРОДНОГО ПАРКУ "ХОЛОДНИЙ ЯР"

Background. In light of the global climate crisis and the military actions undertaken on Ukrainian territory, the conservation of biodiversity has assumed a heightened significance in recent years. A prerequisite for the conservation of fungi listed in the Red Data Book of Ukraine within protected areas is the research and inventory of their species composition within these areas. This is also the case for fungi found at Kholodnyi Yar National Nature Park, but detailed information about their habitat is currently absent in the literature. Methods. The material was collected by the route method. All finds were photographed without removal from nature. For some species, small pieces of fruiting bodies were selected for isolation into pure culture. To verify the species identification of Morchella crassipes samples, which were previously identified by using light microscopy based on morphological characteristics, molecular genetic methods were used, including DNA extraction, amplification of the marker sequence, sequencing, and BLAST analysis of the sequences. Results. The study, conducted between 2017 and 2024, revealed the presence of 12 species of rare fungi within the park. These included Amanita solitaria, Boletus aereus, Grifola frondosa, Helvella monachella, Hericium coralloides, H. erinaceus, Morchella crassipes, Mutinus caninus, Myriostoma coliforme, Plectania melastoma, Polyporus umbellatus, and Sparassis crispa. Two species, Boletus aereus and Myriostoma coliforme, are newly documented in the Right Bank Forest-Steppe. Six species were identified for the first time in the Cherkasy region (Boletus aereus, Helvella monachella, Hericium erinaceus, Myriostoma coliforme, Plectania melastoma, and Sparassis crispa). The article provides information about the locations where the fungi were discovered and includes original photographs. Additionally, it offers a summary of the distribution of these species in Ukraine. Сonclusions. The article presents for the first time generalized data on the distribution of fungi listed in the Red Data Book of Ukraine within the Kholodnyi Yar National Nature Park. The frequency of findings and the number of fruit bodies indicate irregular fruiting or collection of these fungal species by the population.

Identification and functional study of AA11 family polysaccharide monooxygenase genes in filamentous fungus Podospora anserina.

The lytic polysaccharide monooxygenase (LPMO) in the auxiliary active protein family (AA family) catalyzes the oxidative depolymerization of various refractory carbohydrates including cellulose, chitin and starch. While accumulating studies investigate the enzymology of LPMO, the research on the inactivation of LPMO genes has been rarely explored. In this study, five LPMO genes PaLPMO11A (Pa_4_4790), PaLPMO11B (Pa_1_5310), PaLPMO11C (Pa_2_7840), PaLPMO11D (Pa_2_8610) and PaLPMO11E (Pa_3_9420) of the AA11 family in the filamentous fungus Podospora anserina were knocked out by homologous recombination. Single mutants ΔPaLPMO11A (ΔA), ΔPaLPMO11B (ΔB), ΔPaLPMO11C (ΔC), ΔPaLPMO11D (ΔD) and ΔPaLPMO11E (ΔE) were constructed, and then all polygenic mutants were constructed via genetic crosses. The differences in the growth rate and sexual reproduction between wild type and mutant strains were observed on different carbon source media. The alteration of oxidative stress and cellulose degradation ability were found on DAB and NBT staining and cellulase activity determination. These results implicated that LPMO11 genes play a key role in the growth, development, and lignocellulose degradation of P. anserina. The results showed that the spore germination efficiency, growth rate and reproductive capacity of mutant strains including ΔBΔCΔE, ΔAΔBΔCΔE, ΔAΔCΔDΔE and ΔAΔBΔCΔDΔE was significantly decreased on different cellulose carbon sources and the remaining strains have no difference. The reduced utilization of various carbon sources, the growth rate, the spore germination rate, the number of fruiting bodies, the normal fruiting bodies, the shortened life span and the ability to degrade cellulose were found in strains which all five genes in the PaLPMO11 family were deleted. However, the strain still had 45% cellulase activity compared to wild type. These results suggest that LPMO11 genes may be involved in the growth and development, sexual reproduction, senescence and cellulose degradation of P. anserina. This study provides information for systematically elucidating the regulatory mechanism of lignocellulose degradation in filamentous fungus P. anserina.

Usability of Zea mays and Sorghum bicolor on the growth and yield of oyster mushrooms

In this research, three agricultural substrates, Zea mays, Sorghum bicolor Horse S, and Sorghum bicolor Giza 115, were evaluated as growing media in commercial mushroom cultivation. Zea mays waste had the highest moisture content with a relatively high nitrogen, phosphorus, potassium, protein, and energy value compared with Sorghum bicolor Horse S and Sorghum bicolor Giza 115, so the results indicate that Zea mays waste cultivated with different species of Pleurotus tested gave high-production oyster mushrooms with the highest biological efficiency and also a high total number of fruit bodies. P. floridans cultivated in Zea mays waste gave the best production by 182 gm, biological efficiency of 36.4%, and also a high number of fruit bodies (28) followed by P. pulmonarius on Sorghum bicolor Horse S waste, which gave production by 180 gm and also a high number of fruit bodies (27).

Utilization of Wastes Lemon Grass (Cymbopogon citratus) Substrates in the Cultivation of Oyster Mushroom: Modern View and Trends

Lemon grass wastes are abundant in both rural and urban areas and when carelessly disposed off to the environment by dumping or burning, they lead to environmental pollution, and consequently to health hazards. Growing of edible mushroom on these wastes can contribute to decrease in environment pollution. The aim of this study was conducted to compare the wastes lemon grass (cymbopogon citratus) substrates in the cultivation of Pleurotus sajor-caju and Pleurotus florida. Six substrate, namely, Trt1 (oil extracted lemongrass), Trt 2 (oil extracted lemon grass 40% + wheat straw 30%+ paddy straw 30%), Trt3 (oil extracted lemongrass 70% + wheat straw 30%), Trt4 (oil extracted lemon grass 50% + wheat straw 50%), Trt5 (oil extracted lemongrass 70% + paddy straw 30%) and Trt6 (oil extracted lemongrass 50% +paddy straw 50%) in the cultivation of PSC and PF. The results indicated that different substrate formulas gave a significant difference in complete full spawn run and days to pinhead formation were faster in Trt2 (15.5 and 13.70 days) and ( 18.40 and 17.40 days respectively) while Trt1 recorded the longest days (23.64 and 22.37) and (27.00 and 25.64 respectively) in PF and PSC. Number of fruiting bodies, fresh and dry weights of the mushroom was significantly higher in Trt2 than in the other substrates. The least number of fruiting bodies, fresh and dry weights were recorded in Trt1 of PF and PSC. The treatment Trt2 showed all over best result produce 884.37g.and 856.33g in PSC and PF. but as far as lemon grass (Trt1) alone and its combined effect all other treatments. Combination of lemon grass with wheat straw + paddy straw also showed significant produce while, using oil extracted lemongrass 100% does not show better results.

The Effect of Banana Stem Powder on the Productivity of the Brown Oyster Mushroom (Pleurotus cystidiosus)

Background: Brown Oyster Mushroom (Pleurotus cystidiosus) is a type of mushroom that has different advantages from other oyster mushrooms. Generally, farmers make planting media for brown oyster mushrooms made from sawdust of sengon wood. The availability of raw materials for sawdust of sengon wood is increasingly difficult to obtain. So we need other alternative materials as a source of nutrition, one of which is banana stem waste. This study aims to determine the effect of banana stem flour on the diameter of the cap, the number of fruiting bodies, and the wet weight of the fungus. Methods: This study used a completely randomized design (CRD) which consisted of one factor. This study used 4 treatments and 3 replications. Data was tested by quantitative descriptive analysis using the Analysis of Variance (ANOVA) test. Results: Banana stem flour has a significant effect on all parameters. The JM2 treatment resulted in a cap diameter of 10.3 cm, mushroom fruiting bodies of 10.6 and wet mushroom weight of 103.8 g. Conclusion: The addition of banana stem powder can increase productivity in the parameters of mushroom cap diameter, number of mushroom fruiting bodies, and wet weight of mushrooms, which can be used by readers or researchers. Utilization of waste such as sawdust of sengon wood and waste of banana fronds for the cultivation of brown oyster mushrooms is one way of handling plantation waste.

Commercial scale production of Yamabushitake mushroom (Hericium erinaceus (Bull.) Pers. 1797) using rubber and bamboo sawdust substrates in tropical regions

Yamabushitake (Hericium erinaceus) is one of the most sought out mushrooms that is widely used for both direct consumption and medicinal purposes. While its demand increases worldwide, cultivation of the mushroom is limited to temperate areas and its production in tropical regions has never been explored. The aim of this study was to test the utilization of rubber and bamboo sawdust, alone or as a substrate mixture, for industrial scale Yamabushitake mushroom production. Five substrate treatments with various ratios of the two sawdust were compared for their physicochemical properties in relation to mushroom productivity. The highest mushroom fresh and dry (113.22 and 23.25 g, respectively), biological efficiency (42.61%), and cap size (9.53 cm) were obtained from the substrates containing 100% rubber sawdust, with the mushroom yield decreasing proportional to the ratio of bamboo sawdust. The 100% rubber sawdust substrate provided a higher initial organic matter and carbon content together with C:N ratio at 63.2%, 36.7% and 65.48, respectively, whereas the 100% bamboo sawdust provided higher nitrogen content (1.03%), which was associated with lower mushroom yield but higher number of fruiting bodies. As in the 100% rubber sawdust substrate, a comparable mushroom yield and growth attributes were also obtained in the 3:1 rubber-bamboo sawdust mixture substrate. Principle component analysis of the measured variables indicated a strong influence of substrate C:N ratio before spawning and the change in substrate electrical conductivity and N content after cultivation to the variation in mushroom productivity among the treatments. The results demonstrate the applicability of rubber sawdust and its combination with up to 25% of bamboo sawdust for Yamabushitake mushroom cultivation and provide the basis for substrate optimization in the tropical Yamabushitake mushroom industry through a circular economy framework.

Performance of Edible Mushroom Varieties Under Different Agricultural Substrates

The study was conducted at Dr. Emilio B. Espinosa, Sr. Memorial State College of Agriculture and Technology (DEBESMSCAT) Cabitan, Mandaon, Masbate. The experimental design used was 3 x 7 CRD factorial with three varieties of mushrooms (Pink Oyster, White Abalone, and Milky Mushroom), seven agricultural substrates as treatments, Treatment A (70% rice straw and 30% sawdust), Treatment B (70% rice straw and 30% corn cob), Treatment C (70% rice straw and 30% Carbonized Rice Hull), Treatment D (100% rice straw), Treatment E (100% sawdust), Treatment F (100% corn cob) and Treatment G (100% Carbonized Rice Hull). The experiment was replicated three times with a total of 945 fruiting bags. Height, fresh weight, and the number of fruiting bodies are the data that was observed in the study. The result showed that in terms of growth performance, a nonsignificant result was observed in the height performance of the mushroom varieties inoculated in substrates with 70% rice straw, 30% corn cob, and 100% carbonized rice hull. Similarly, the number of mushroom fruiting bodies produced differed among mushroom varieties inoculated. Mushrooms inoculated in 100% carbonized rice hull attained more fruiting bodies than other tested varieties. Mushrooms inoculated in 100% corn cob produced a significantly higher number of mushroom fruiting bodies than other treatments. Finally, the weight of mushroom fruiting bodies produced in 70% rice straw and 30% carbonized rice hull applications was statistically significant for all agricultural substrates inoculated. The researcher concluded that using agricultural substrates other than carbonized rice is effective in mushroom production.

The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development of Flammulina filiformis.

Flammulina filiformis is one of the most commercially important edible fungi worldwide, with its nutritional value and medicinal properties. It becomes a good model species to study the tolerance of abiotic stress during mycelia growth in edible mushroom cultivation. Transcription factor Ste12 has been reported to be involved in the regulation of stress tolerance and sexual reproduction in fungi. In this study, identification and phylogenetic analysis of ste12-like was performed by bioinformatics methods. Four ste12-like overexpression transformants of F. filiformis were constructed by Agrobacterium tumefaciens-mediated transformation. Phylogenetic analysis showed that Ste12-like contained conserved amino acid sequences. All the overexpression transformants were more tolerant to salt stress, cold stress and oxidative stress than wild-type strains. In the fruiting experiment, the number of fruiting bodies of overexpression transformants increased compared with wild-type strains, but the growth rate of stipes slowed down. It suggested that gene ste12-like was involved in the regulation of abiotic stress tolerance and fruiting body development in F. filiformis.

Role of Mushroom Pathogen Trichoderma harzianum Rafai in the Reduction of Growth Yield, Nutrient Quality, and Morphometric Measurements of the Fruiting Body of Pleurotus ostreatus EM-1 From Seven Oyster Mushroom Cultivation Farms in Ghana.

The cultivation of edible mushrooms such as Pleurotus ostreatus commonly known as oyster mushroom has been undertaken by both commercial and cottage industry entrepreneurs in Ghana with appreciable success. However, the presence of competitors and potential pathogens in the substrate militate against increased yield and quality of fruiting bodies. The most prevalent pathogen Trichoderma harzianum in the compost for cultivating P. ostreatus is responsible for the green mould disease. In this study, in vitro pathological effects of spiking Triplochiton scleroxylon sawdust substrate with T. harzianum on pinhead emergence, percentage formation of fruiting bodies, weight of fruit body, yield, Biological Efficiency as well as morphometric data of stipe length, stipe diameter, cap diameter as criteria for reduced growth performance of P. ostreatus were investigated. The effect of the pathogen on the nutritional and elemental composition of the oyster mushroom were determined with the view of assessing the quantitative changes of nutritional and elemental composition of the mushroom using standard methods. Growth substrates inoculated with T. harzianum reduced pinhead formation; number of fruiting bodies formed, percentage conversion of pinheads, weight of fruiting bodies, and reduced morphometric measurements of stipe length, stipe diameter, cap diameter with all three flushes of the mushroom. Total yield of fruiting bodies in all three flushes in the T. harzianum-inoculated bags decreased by 67.4%, 68.5% and 83.3% respectively with the concomitant reduction of total yield by 52.3% and Biological Efficiency by 52.8%. Some of the control bags (uninoculated bags) also performed poorly as a result of contamination by T. harzianum, indicative of inadequate sterilization. Fruiting bodies of P. ostreatus grown in uninoculated compost contained Ca, Cu, Fe, K, Mg, Na, P, and Zn in contrast with the absence of Na, and Zn in the infected bags. There was a reduction in fat, crude fibre, crude protein, and carbohydrates of the fruiting bodies grown in the contaminated bags. On the other hand, total ash content was doubled in the carpophore from the T. harzianum spiked contaminated bags. The pathogen therefore has adverse effect on the production and marketing quality as well as the nutritional value of the fruiting bodies

ASSESSMENT OF GROWTH AND YIELD PARAMETERS OF MUSHROOM SPECIES (Pleurotus Eryngii and Pleurotus Ostreatus) AS INFLUENCED BY DIFFERENT SUBSTRATES

This experiment was carried out to determine the influence of three substrates on the growth and yield of Pleurotus eryngii and Pleurotus ostreatus in 2022 at the laboratory of Faculty of Agriculture, COOU, Igbariam, Anambra State. This study was designed out as a 3×2 factorial experiment in a completely randomized design. The substrates (rice bran, corn chaff, groundnut cake) were the main factor while the mushroom species (P. eryngii and P. ostreatus) were the sub-factor. Data were collected on spawn run period, pinhead initiation, total number of fruiting bodies, maximum and minimum weight of fruiting bodies, length and width of stalk, diameter of mushroom cap, yield of first three flushes, total yield and biological efficiency. The data collected above were subjected to analysis of variance. Significant means were separated using Fisher’s Least Significant Difference. The results obtained indicated that rice bran substrate gave the highest yield (10.70g), and biological efficiency (6.20%). This was followed by corn chaff while, groundnut cake had the lowest growth and yield values. The results also showed that P. ostreatus specie produced highest or maximum weight of fruiting bodies (4.88g), better cap diameter (3.10cm), stalk length (3.70cm), stalk width (1.20cm) and yield (40.67g) followed by P. eryngii.