Number Of Flowers Research Articles

Functional characters and ontogenetic structure of populations of Ziziphora clinopodioides (Lamiaceae) at the range border (Republic of Khakasia)

The object of this study is a characteristic subshrub of Khakasia, Ziziphora clinopodioides (Lamiaceae). The study of functional traits and the structure of the Z. clinopodioides populations at the eastern limit of its distribution (Republic of Khakasia) has been carried out for the first time. The materials were collected in 2022–2023 in true and meadow steppes of the forest-steppe belt. Environmental factors influencing functional traits and the ratio of individuals of different ontogenetic states have been identified. It has been established that disturbed steppes on sandy soils with the presence of stones and low moisture represent the ecological optimum for this species. Under these conditions, the indices of most of the studied functional traits (the number of vegetative shoots per 1 composite skeletal axis, the number of generative shoots with paracladia, the number of paracladia, the number of flowers in paracladia, the number of flowers in the main inflorescence) reach the highest values. In undisturbed steppes on sandy loam soils with moderate moisture, Z. clinopodioides is in unfavorable conditions, and develops individuals with minimal values of functional traits. All the studied populations are normal, complete or incomplete. Left-sided and bimodal ontogenetic spectra are formed in disturbed steppes on sandy soils with the presence of stones and low moisture; in the undisturbed steppe on sandy loam soil with moderate moisture, the spectrum type is bimodal. The density of individuals depends on the degree of soding in the community and anthropogenic load. With an increase in the total projective cover and in the absence of anthropogenic impact, the density of individuals decreases. The causes of restructuring of functional characters and changes in the ontogenetic spectrum of populations of Z. clinopodioides under the influence of various environmental factors at the range border are discussed.

Demonstration of Local Honeybee Flora “Mentesie” (<i>Becium grandflorum</i>) Propagation Techniques in Wag - Himra Zone, Ethiopia

The demonstration of local honeybee flora "Mentesie" (<i>Becium grandiflorum</i>) propagation techniques was carried out over two rainy seasons in the Gazgibla district, Debreweyla watershed closure area of the Northeastern Amhara region. The shrub has numerous benefits for the community, including honey production, fuel use, and soil and water conservation. However, it has been gradually declining. To preserve these valuable shrubs, various propagation techniques were tested. The objectives of the experiment were to demonstrate the propagation techniques of <i>Becium grandiflorum</i> to beekeepers and to generate wider demand for its plantation among beekeepers and extension workers. The experiment compared two selected propagation techniques with local farmers’ traditional planting practices, under the guidance of forestry researchers. The treatments included seeds and cuttings. Seeds were collected from mature fruits, and cuttings were taken early in the morning from natural, mature, young, and healthy mother plants at a height of 40 cm during 2020/21. These materials were packed in perforated polyethylene bags. The average mean of flower numbers, branch numbers, and canopy cover for the enhanced propagation techniques (seedlings with pots) were 4107, 21.81, and 92.5, respectively. These values were higher than those for the comparative propagation technique, which had flower numbers of 2462, branch numbers of 19.4, and a canopy cover of 92.5. There were significant differences (p<0.05) among the propagation techniques in terms of canopy cover, height, number of flowers, and branches per plant. Farmers recognized the higher flower biomass, canopy cover, and potential for greater plant height of the enhanced techniques, which are beneficial for bee forage and soil and water conservation. Therefore, planting seedlings using pots were found to be the best method for the wider production of <i>B. grandiflorum</i> in its natural growing areas.

A new species of Crinum (Amaryllidaceae) from the Eastern Ghats of Andhra Pradesh, India

Crinum andhricum (Amaryllidaceae), a new species from the Northern Eastern Ghats of Andhra Pradesh is described and illustrated. It is closely related to Crinum amoenum and C. stracheyi, but differs in its number of flowers per inflorescence, shape of the perianth lobes and filament length.

Optimizing Vegetative Growth and Flowering in Fuchsia x hybrida: The Role of Media, Pinching, and Growth Regulators

The present investigation was carried out at the experimental farms of Horticultural Regional training station and Krishi Vigyan Kendra at Kandaghat (Solan) Himachal Pradesh, India under polyhouse conditions. It was laid out in a Completely Randomized Design (CRD) with 24 combinations of growing media M1: Soil+ Sand+ FYM (1:1:1v/v), M2:Cocopeat+Soil+Sand+ Leafmould+FYM (2:1:1:1v/v), M3:Cocopeat+Perlite+Vermiculite+Vermicompost (2:1:1:1 v/v), M4:Cocopeat + Vermicompost + Leafmould (2:1:1 v/v), growth regulator [G1: Control (0 ppm), G2:Benzyl adenine (150 ppm),G3: CPPU (10 ppm)] and pinching methods [P1:Single pinch and P2:Double pinch] which were replicated thrice each having 10 pots accommodating one plant per pot. Plants were raised through terminal cutting and were planted in 6-inch size pots. Study revealed that maximum plant height (33.13 cm), plant spread (33.37 cm), length of side shoots (24.67 cm) and minimum number of days taken for visible bud formation (92.40 days), number of days taken for peak flowering (160.60 days) was recorded when the single pinched plants were grown in media consisting cocopeat + Vermicompost + Leafmould (2:1:1 v/v) and sprayed with 150 ppm of benzyl adenine. However, maximum number of side shoots (27.33), number of flowers per plant (55.67), number of flowers per plant open at a time (42.93), duration of flowering (24.67days) was recorded when double pinched plants were grown in growing media consisting of cocopeat + vermicompost + Leafmould (2:1:1 v/v) and were sprayed with 150 ppm of benzyl adenine. The maximum flower diameter (4.61 cm) was recorded when single pinched plants were grown in medium consisting cocopeat + vermicompost + leafmould (2:1:1 v/v) and sprayed with 10 ppm of CPPU. Based on the present investigation findings, double pinched plants of Fuchsia x hybrida grown in pots consisting of growing media i.e., cocopeat + vermicompost+ leafmould (2:1:1 v/v), sprayed with 150 ppm of benzyl adenine is recommended to obtain quantitative and attractive pot plants of Fuchsia x hybrida.

Unveiling the Spectacular Performance of Diverse Chrysanthemum Varieties in Flowering, Quality and Yield

A comprehensive research initiative conducted in Jhalawar 2019-20 focused on evaluating the genetic diversity and performance of various chrysanthemum varieties. The aim of this study was to evaluate the genetic variability and assess the varietal performance of chrysanthemum (Dendranthema grandiflora Tzvelev) under the specific agro-climatic conditions of Jhalawar. The study sought to identify high-performing varieties and quantify key genetic traits relevant to yield and quality characteristics, with the goal of supporting selection efforts for enhanced chrysanthemum production in the region. This investigation was conducted at the Floriculture and Landscaping block of the College of Horticulture and Forestry, Jhalawar, Rajasthan. The experimental work was carried out during the winter season of the year 2019-20, capitalizing on the local climate to evaluate varietal responses under Jhalawar conditions. The experiment employed a Randomized Block Design (RBD) with multiple replications to ensure robust data. Different chrysanthemum varieties were planted in designated plots, and observations on traits such as flower yield, flower quality, and growth parameters were recorded periodically. Statistical analysis, including ANOVA, was performed to detect significant differences among the varieties. This analysis helped in identifying high-performing varieties suited for Jhalawar conditions and provided insights into genetic variability for potential selection and breeding programs. Ten different genotypes were carefully chosen for the study, including Marigold, Poornima Pink, Dall White, Calcutta Shantini, Karnool, Poornima Red, Dall Yellow, Poornima White, Poornima Yellow and Dundi for in-depth analysis.A randomised block design with three replications was used in the experiment. Cultivar 'Calcutta Shantini' exhibited the maximum days taken to first flower bud emergence of plant (51.50 days) and the maximum number of flowers per plant (269.90). Conversely, cv. 'Dall Yellow' showed the shortest duration to fifty percent flowering (67.64 days). Notably, cv. 'Dundi' displayed the maximum flower diameter (7.47 cm), while cv. 'Poornima Pink' had the longest flower stalk (13.09 cm). Remarkably, cv. 'Marigold' demonstrated the highest flower fresh weight (63.67 gm), dry weight (14.41 gm), and shelf life (4.67 day).

The Effects of Tomato Intercropping with Medicinal Aromatic Plants Combined with Trichoderma Applications in Organic Cultivation

To increase biodiversity in tomato cultivation, two herbal aromatic plants, thyme (Thymus vulgaris) and basil (Ocimum basilicum L.), were introduced as companion plants. Their role was to improve crop plant growth and stress resistance. Moreover, the effect of the soil application of Trichoderma microbial preparations on tomato growth parameters and yield, in combination with companion plants, was studied. Ligno-cellulose multi-layer microcapsules with Trichoderma atroviride TRS14 spores (MIC14) and the commercial preparation Trianum G (TG) were used as microbial preparations. This experiment was carried out in a certified organic field. Tomato plants were intercropped with thyme or basil in the arrangement of two tomato rows alternating with one herbal row. In all intercropping arrangements and in the control (tomato plants grown without herbs), subplots were sectioned. The soil in the subplots was amended with the MIC14 and TG preparations used at a concentration of 104 spores g−1 of the soil and planted with tomato transplants. No control measures were applied during tomato growing, and the plants were naturally infected with late blight. Tomato plant growth parameters and yield were assessed, and late blight severity was monitored. The degree of soil colonization by Trichoderma fungi and the effect of these applications on soil microbial activity and biodiversity (dehydrogenases activity, EcoPlates AWCD, and Shannon index) were evaluated. The results clearly showed a significant influence of thyme and basil on tomato growth and yield in organic production. The cultivation of thyme adjacent to tomatoes had a beneficial effect on the development of the root system and the number of flowers and fruits on the crop plants. Basil, on the other hand, clearly decreased tomato yield and adversely affected the effect of Trichoderma applications by reducing root system development. Moreover, basil as a companion plant increased late blight symptoms. Both Trichoderma strains colonized soil, but they had no significant effect on the microbial activity or metabolic potential measured on the EcoPlates with the use of the BIOLOG system. However, a decrease in dehydrogenases activity was noted. In organic cultivation, the Trichoderma preparations used had no significant effect on tomato yield, opposite to its increase in integrated tomato production.

Triploid Formation and Heat Tolerance of Angelonia angustifolia with Various Ploidy Levels

Angelonia (Angelonia angustifolia), a popular potted flower and bedding plant, often experiences severe wilting or leaf drying during hot summers in tropical and subtropical regions. Polyploidization may enhance the heat tolerance of angelonia plants. In this study, diploid (2x) angelonia ‘Serena Purple’ and ‘Serena White’ were crossed with tetraploid (4x) lines to produce interploidy plants. More seed production was observed from 4x × 2x crosses than from 2x × 4x crosses. Despite observing triploid block in interploidy hybridization, triploid plants were successfully obtained from the interploidy seeds. Growth, flowering, and photosynthesis of diploid, triploid, and tetraploid plants were assessed at day/night temperatures of 30/25 °C and 25/20 °C. All angelonia plants exhibited increased height and stem diameter as well as reduced leaf SPAD-502 value, leaf thickness, flower number, flower length, net photosynthetic rate, and stomatal conductance at 30/25 °C compared with those at 25/20 °C. At 30/25 °C, triploid and tetraploid plants had higher leaf SPAD-502 values, leaf thickness, and net photosynthetic rates than those of diploid plants. Diploid plants had the most branches at 25/20 °C, but their branch and flower numbers decreased the most at 30/25 °C compared with those of triploid and tetraploid plants. Notably, at 30/25 °C, triploid plants exhibited the earliest f lowering and produced the most branches and flowers compared with those of diploid and tetraploid plants.

Inoculation of Arthrobacter sp. improves the growth of Acanthocalycium sp. and the fruits nutraceutical quality and the flowers longevity

Research objective: The aim of this work is on the inoculation in the growing medium of Arthrobacter sp. and its effects on the growth, flower quality and longevity of cactus plants. To this end, the study of biometric parameters, i.e. plants and flowers (height, weight, number and duration), as well as root growth (weight), and the presence of disease were quantified as measures of plant productivity and compared with those obtained from non-inoculated plants. The use of alginate microspheres as a means of bacterial inoculation was also considered. Materials and Methods: Seedlings of Acanthocalycium (2 years old) Ferrarii, A. Glaucum and A. Violaceum were immediately planted in pots after purchase, in substrate containing (sand 20%, pumice 20% and peat 60%; pH 7; electrical conductivity 0.7 dS/m; total porosity 80% (v/v). A randomised complete block design was applied for the experiments: the pots were randomly divided into two series, one series was inoculated with the bacteria and the other non-inoculated was used as a control. Each month, plant growth was assessed according to (1) plant height, (2) vegetative and root weight, (3) number of flowers per plant (4) flower duration. In addition, the occurrence of possible diseases, in particular Fusarium sp. and Verticillium sp., was assessed. Total flavonoids, total phenols and antioxidant activity were also analysed. Results and Discussion: The experiment showed that the use of Arthrobacter sp. can indeed significantly improve the vegetative and root growth of Acanthocalycium sp. cacti. In addition, there was a significant improvement in the number and floral longevity as well as in the phenol, flavonoid and antioxidant content of the fruits of the inoculated plants compared to the untreated control. The experiment also showed that mortality due to the pathogens Fusarium sp. and Verticillium sp. was significantly reduced in the treated plant. In agreement with these authors, we found that PGPB treatments increased agronomic parameters and improved Fusarium and Verticillium resistance. In general, the application of biostimulants can increase the synthesis of bioactive compounds in plants by increasing resistance to phytopathologies. Inoculation with Arthrobacter sp. led to a significant reduction in the number of dead plants with respect to the diseases analysed. As a result of the stimulating action demonstrated in Acanthocalycium, bioproducts suitable for nutraceutical purposes may be developed in the future. Therefore, new microbe-assisted technologies can help plants to resist stress conditions, improving their tolerance and productivity. Conclusions: Due to its ‘multifunctionality’, the genus Acanthocalycium is considered as one of the species of the future, for ornamental use and medicinal aspects, and new results may help reveal its potential in the context of the bio-economy and circular economy. As natural resources and cultural practices are crucial in defining the quality of flowers when destined for food/nutraceutical applications, the inoculation of Acanthocalycium with Arthrobacter sp. can be envisaged to provide better plant growth under conditions of environmental stress, or as a soil fertiliser, but also to improve the synthesis of natural products used for therapeutic applications.

Plant traits and seasonality shape coexistence and niche segregation patterns among spider species.

Understanding the mechanisms that enable species coexistence is a central question in ecology, as it helps to comprehend species diversity. One of the most common stabilizing mechanisms of coexistence is niche segregation, which can prevent the competitive exclusion of the fittest competitor. Niche segregation can manifest itself at various temporal and spatial scales, allowing provide essential insights into understanding the stabilizing mechanisms facilitating the coexistence of species. We assessed coexistence patterns among flower-dwelling spiders in two ways, in the first set of analyses, we investigated the factors influencing the quantity of spider individuals and species. The second approach we investigate the spatio-temporal segregation between species, effectively examining the coexistence patterns. We observed that the presence of inflorescences per plant, the number of flowers per inflorescence, and the presence of EFNs play a significant role in increasing spider abundance and richness. We find only a marginal seasonal effect, suggesting that spiders have constant access to resources throughout the year. Our niche overlap analysis demonstrated synchrony in the spatial occupation of niches by different spider species. The coexistence patterns appeared to be unaffected by the number of inflorescences. The greater number of inflorescences will enable a greater availability of niches, and consequently more abundance and richness of species of spiders the plant can sustain. Our results suggest that, to mitigate the adverse consequences of competitive interactions, spiders tend to adopt spatial partitioning as a strategy to facilitate the coexistence of spiders living in reproductive structures on plants in the Brazilian savanna.

Determination of Local Cherry Tomato Genotypes' Agromorphological Features in Türkiye and Kyrgyzstan

The purpose of this study was to ascertain some morphological traits and interrelationships of 36 genotypes of cherry tomatoes that were gathered from Turkey and Kyrgyzstan. Measurements and observations include flower number of cluster, leaf length, leaf width, leaf attitude, fruit color, fruit neck shape, fruit cross-sectional shape, fruit weight, fruit firmness, time of maturity, number of locules, thickness of pericarp, and total soluable solid content, in accordance with the guidelines of the International Union for the Protection of New Varieties of Plants (UPOV). The genotypes T40 (62.7 g), T24 (50.8 g), T107 (50 g), T103 (41.4 g), and T105 (40.8 g) had the highest average fruit weights, whereas T3 (9.4 g) and K1 (10.6 g) had the lowest fruit weights. The pericarp had an average thickness of 3.82 mm and an average of 2.49 locules. A spherical fruit cross-section was observed in 97.2% of genotypes. Genotype K2 had the highest total soluble solids content (TTSC%) at 9.4%, whereas genotype K13 had the lowest TTSC% at 3.1%. To measure the data, Principal Component Analysis (PCA) was used. Five separate principle component axes were identified via PCA, and these axes together account for 75.57% of the dataset's variation. These principal components' eigenvalues, which varied from 1.07 to 3.24, showed how much variance was accounted for by each component. The PCA results showed that the genotypes T107, T40, T105, and T87 performed better in PC1 in terms of the number of locules, fruit weight, fruit cross-sectional shape, and pericarp thickness characteristics. Fruit color was influenced by genotypes T90 and T142, fruit firmness by genotype T40, and total soluble solids content by genotype T98. The indigenous cherry tomato varieties from Turkey and Kyrgyzstan were shown to have a large amount of morphological variety, which may be useful for breeding initiatives in the future.

Potassium Fertilizer Application Rates for Fertigated Edamame Grown on Low-K Soils

Edamame, a legume consumed fresh as a vegetable, is highly nutritious, particularly protein-rich, and holds significant economic value. However, its cultivation faces challenges, especially on dry land, due to water scarcity and limited nutrient availability, particularly potassium (K). This study, which investigated the impact of potassium fertilization rate on edamame cultivation, underscores the need for further research. The study utilized a single factor, potassium fertilization rate, arranged in a completely randomized block design. Potassium rates consist of 0% X, 50% X, 100% X, 150% X, and 200% X, where X represents the recommended potassium fertilization rate according to the dry soil test device (DSTD) guidelines. Each treatment was replicated five times. Data were analyzed using ANOVA at a 5% significance level, and any significant effects were further examined using orthogonal polynomial and regression analysis. The results indicated that potassium fertilizer rates did not significantly affect edamame height, pod weight per plot, and marketable yield. However, the study identified the optimal potassium fertilizer rate, which was between 83%X and 119%X, equivalent to 83–119 kg.ha⁻¹ of KCl (50–72 kg.ha⁻¹ of K₂O). This range positively increased total branch yield, productive branches, number of flowers, pod weight per plant, number of pods per plant, and plant dry weight, producing a quadratic response pattern. The study recommends further research to optimize potassium fertilizer doses based on DSTD recommendations, particularly at a low K nutrient status, to maximize marketable yields through fertigation.

Assessment of Standard ChrysanthemumGenotypes for Morphological and Floral Attributes in Terai Region of West Bengal, India

Aims: Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the most economically important and economically favoured floricultural crops used as cut flower, loose flower, potted plants, bedding and border plants, making garlands, flower decoration, bracelet, venis and religious offerings. The present study aimed to evaluate and select suitable large flowering chrysanthemum cultivars for commercial cultivation in Terai region of West Bengal. Location of the Study: The experiment was conducted in the Instructional Farm, Department of Floriculture, Medicinal and Aromatic Plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya during the period of September to February of 2022-23 and 2023-24. Materials: Thirty five different cultivars of large flowering chrysanthemum were evaluated based on their growth and flowering parameters viz. Angel Bell, Autumn Blaze, Autumn Day Light, Bolare Deo, Cassa Granda Yellow, Chengis Khan, Coronation Gold, Cossak, Diamond Jubilee, Dignity, Dream Castle, Golden Yellow, Green Goddess, Innocence, J.S.Loyed, Jane Sharp, Jessie Hab Good, Kasturba Gandhi, Kenroken Pink, Kenroken White, Kikubiory, Lady Frank Klerk, Phil Houghton, Pink Cloud, Pinkling, Raja, Red Wine, Royal Prince, Royal Purple, Silk Brocade, Snowball, Sonar Bangla, Temptation, Willium Turner and Yellow Reonet. Methodology: One month old rooted cuttings were planted at a spacing of 40 cm X 30cm (Row to row 40cm and plant to plant 30cm) in 1.6m X 1.2 m raised bed. Total 16 plants were planted in one plot. Statistical Design: The experiment was laid out in completely randomized block design replicated thrice. Collected data was analyzed by one-way analysis of variance with the help of SPSS software. Results: Significant variation was observed among the genotypes. Maximum plant spread (N-S) of 36.33±5.90, 39.00±1.55 and 37.67±4.13 cm was observed in Snowball in the year 2022-23, 2023-24 and pooled respectively whereas minimum plant spread (N-S) of 16.42±1.73 cm was observed in Cassa Granda Yellow. The maximum number of flowers per bed 282.74, 291.34 and 287.04 was recorded in Pinkling during the first year, second year, and pooled data, respectively, while the minimum number of flowers per bed (140.60 and 149.55) was observed in Autumn Blaze during the first year pooled data, respectively while in second year Pink Cloud (145.40) recorded the minimum numbers of flowers per bed. Conclusion: From the study, it may be recommended that the cultivars like Snowball, Sonar Bangla, Pinkling, Temptation are suitable for cut flowers. The cultivars namely Silk Brocade, Pink Cloud, Diamond Jubilee, Corronation Gold, Innocence, Kenroken White and Kenroken Pink may be selected for pot plants and for garden display due to profuse uniform branching and blooming.

Growth and initiation of third stage of immature oil palm (Elaeis guineensis Jacq.) by giving oil palm frond compost and paclobutrazol

The third stage of immature oil palm is the phase where the plants enter preparation for flowering, so efforts are needed to initiate flowering, one of which is by using plant growth regulators (PGR) such as paclobutrazol. It is expected that the use of oil palm fronds as compost and application to oil palm plants can support sustainable oil palm management activities. Paclobutrazol, as a growth regulator, plays a role in stimulating the flowering of immature oil palm plants. This research aims to determine the response to the growth and development of immature oil palm plants in the third stage by administering palm frond compost and paclobutrazol at certain doses and concentrations. The experiment was carried out from June to December 2020 at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University. The experimental design used was a Randomized Block Design (RBD) with 12 treatments and three replications. The experimental results showed that the application of compost to oil palm fronds and paclobutrazol had a significant effect on plant height and stem girth, but there were no significant differences in the number of fronds, leaf area, chlorophyll index, and flowers. All treatments did not produce flower shoots but showed inhibition of vegetative growth in immature oil palm plants in the third stage. The application of 12.8 kg of oil palm frond compost and 150 ppm paclobutrazol had the best effect on the height and girth of the oil palm stem and had an effect that was not significantly different from other treatments on the parameters of a number of fronds, leaf area, leaf chlorophyll, and flower growth.

Difference in reproductive traits of two sympatric species of Stigmaphyllon (Malpighiaceae) throughout their distribution range: Are they context-dependent?

Populations of widely distributed plants may experience varying climatic or ecological conditions, leading to uneven evolution of reproductive traits. However, studies on reproductive biology usually focus on just one, or rarely two, sampling areas. In this study, we performed a geographical comparative analysis of the flowering pattern, pollination biology and breeding system of Stigmaphyllon bonariense and S. jatrophifolium (Malpighiaceae) throughout their natural distribution in Argentina, classifying them as ‘northern’ or ‘southern’ according to their geographic location. For this, we selected 4 populations for S. bonariense and 3 for S. jatrophifolium to characterized the flowering peak at both the individual (IFP) and population (PFP) levels, the floral display per inflorescence, the richness of Malpighiaceae species and their pollinators, and the stigmatic and body pollen loads of the pollinators. In addition, we performed manipulative experiments to determine the breeding system. Our results showed that the duration of flowering phenology and the floral display vary between northern and southern populations of both species. These traits include flowering duration and the number of flowers at anthesis per inflorescence (floral display). In addition, the pollination context exhibited latitudinal differences in species richness, phenology, body size of oil-collecting bees, and visitation frequency. Besides, the number of co-flowering Malpighiaceae species varied among populations. However, no considerable differences were observed among populations in terms of stigmatic and body pollen load in pollinators of both species. The breeding system was invariable and consistent in both species with self-incompatibility (SI). These findings suggest that certain reproductive traits of both Stigmaphyllon species are context-dependent and would allow the species maximise reproductive success in each population. Therefore, our results demonstrate that large-scale geographical studies (i.e., several populations with different climatic conditions) and the analysis of various phenological traits are necessary to better understand the reproductive biology of plant species.

Hengniu: Fast Bearing and High Yielding Coconut Varieties

Benefits of coconut varieties that stakeholders want are early bearing, high yielding, short stems, and slow growth for height. Most local tall coconut palms in Indonesia have stem height above 20 m, making it increasingly difficult to climb to harvest fruit or tapping sap thereby making the harvesting cost high. To breed varieties of desired characters, an evaluation trial was initiated in 2014 with BYD x MTT-S4, RAD x MTT-S4, and KHINA-1 as a control. The crossing was done in 2012 and seedlings planted in January 2014 at Mapanget Experimental Garden, Indonesian Palma Crops Research Institute, North Sulawesi. The study used a Randomized Block Design (RBD) of three types of coconut hybrids, four replications with a plot size of 16 trees. Morphological observations were carried out on stem, crown and leaf characters, inflorescences and flowers, nut production, fruit components, copra, oil content, and fatty acid composition. The results of ANOVA analysis and statistical tests obtained those vegetative characters, such as stem circumference and number of leaf, generative characters namely the number of bunches, first flowering, and fruit production of these three crosses at the age of 4 years after planting, did not show a significant difference. The first initial flowering was in the coconut hybrid of RBD x MTT-S4, which is 26 months, followed by BYD x MTT-S4 in 32 months and KHINA-1 in 36 months after planting. The results of the analysis of fruit and copra production at the age of 5 years showed a significant increase between hybrid coconuts. The highest to lowest fruit production was obtained in BYD x MTT-S4 hybrid coconut, RBD x MTT-S4, KHINA-1, which were 64 nuts, 44 nuts and 26 nuts /palm respectively, or estimated copra production was 2.26 tons, 1.45 tons and 0.88 tons copra/ha. At 6 years old the harvest of fruits from the three hybrid coconuts is obtained sequentially 118 nuts, 99 nuts and 94 nuts/palm. While estimation of copra yield per hectare is found the highest in BYD x MTT-S4 hybrid coconut is 3.86 ton/ha/year and this yield differently significant compare with RBD x MTT-S4 is found 3.04 ton/ha/year, and control hybrid of KHINA-1, which is about 2.74 ton/ha/year. Based on the production potential, hence the estimated optimum production when aged over 10 years can reach more than 5 tons/ha/year. The hybrid coconut variety BYD x MTT-S4 is released in October 2019 under the name HENGNIU.

Evaluation of Agronomic and Genetic Diversity in M2V1 Generation of Marigold (Tagetes erecta L.)

Marigold (Tagetes erecta L.) is an ornamental plant widely used as a potted or cut flower due to its vibrant colors and diverse flower shapes. High genetic diversity is essential for successful breeding programs aimed at developing superior varieties. Genetic information can be obtained by estimating variance components and heritability values, key parameters in plant breeding. A high heritability value suggests that genetic factors influence a plant's characteristics more than environmental factors. This study assessed the genetic diversity and performance of vegetative propagation from 2nd generation mutant plants (M2V1). The research was conducted at the Pasir Sarongge experimental field in Cianjur Regency from July to October 2023, using a randomized complete block design with 17 genotypes and three replicates. The genotypes included 14 from vegetative propagation of M2V1 mutant plants and three comparison varieties: “Maharani”, “BAIP 2”, and “BAIP 3”. Data analysis was performed using PKBT-STAT 3.1 software, followed by the honest significant difference (HSD) test at the 5% level. The results revealed significant differences among genotypes in plant height, stem diameter, canopy width, leaf width, flower weight, flower diameter, number of flowers per plant, total flower weight per plant, and flower stalk length. Floret types observed were either all ligulate or all tubuligulate, with flower colors ranging from orange to yellow to white. Traits such as canopy width, flower weight, flower diameter, and stalk length, which exhibited broad genetic diversity and high heritability, can be used for selection in future marigold breeding programs.

Contribution to the Study of Malagasy Orchids. XXXIX. Two New Species of Bulbophyllum from Madagascar

Two new species of Bulbophyllum Thouars, B. psittacinum Verlynde & Ramand. and B. vespertilioides Verlynde & Ramand. (Orchidaceae), are recognized and described. Both species are endemic to Madagascar and were found in the Ankerana forest, part of the Ankeniheny-Zahamena corridor (CAZ) protected area in the eastern escarpment forests. These two species belong to section Pantoblepharon Schltr.; B. psittacinum differs from the rest of the section by its conjoined lateral sepals and B. vespertilioides by its larger pseudobulbs, longer inflorescence, and number of flowers. Taxonomic treatments of both species with descriptions, illustrations, distribution, and habitat information as well as preliminary IUCN Red List assessments are provided.

Application of machine learning algorithms for predicting the life-long physiological effects of zinc oxide Micro/Nano particles on Carum copticum

Nanoparticles impose multidimensional effects on living cells that significantly vary among different studies. Machine learning (ML) methods are recommended to elucidate more consistence and predictable relations among the affected parameters. In this study, nine ML algorithms [Support-Vector Regression (SVR), Linear, Bagging, Stochastic Gradient Descent (SGD), Gaussian Process, Random Sample Consensus (RANSAC), Partial Least Squares (PLS), Kernel Ridge, and Random Forest] were applied to evaluate their efficiency in predicting the effects of zinc oxide nanoparticles (ZnO NPs: 0.5, 1, 5, 25, and 125 µM) and microparticles (ZnO MPs: 1, 5, 25, and 125 µM) on Carum copticum. The plant root/shoot biomass; number of leaves, branches, umbellates, and flowers; protein content; reducing sugars; phenolic compounds; chlorophylls (a, b, Total); carotenoids; anthocyanins; H2O2; proline; malondialdehyde (MDA); tissue zinc content; superoxide dismutase (SOD) activity; and media ΔpH were measured and considered input variables. All levels of ZnO MPs treatments increased growth parameters compared to the control (ZnSO4). The highest shoot/root fresh and dry mass were recorded at 5 µM ZnO MPs compared with the control. The root fresh/dry mass under ZnO NPs treatments was more sensitive than shoot parameters. The number of flowers increased by 134 and 79% in MPs and NPs treatments compared to the control, respectively. ZnO NPs reduced protein content by up to 81% in 125 µM NPs compared to ZnSO4. Reducing sugar content increased to 25, 40 and 36% in 5, 25, 125 µM MPs and 67, 68, 26, 26 and 21% in 0.5, 1, 5, 25 and 125 µM NPs treatments, respectively. The pH alteration was more significant under NPs and affected zinc uptake. All levels of ZnO NPs treatments increased growth parameters compared to the control. All ML algorithms showed varied efficiencies in predicting the nonlinear relationships among parameters, with higher efficiency in predicting the behavior of root and shoot dry mass, root fresh weight and number of flowers according to R2 index. The model obtained from SVR with the radial basis function (RBF) kernel was selected as a comprehensive model for predicting and determining the efficacy of the results.

Foraging behaviour and pollinator dynamics in lucerne crop of the Bundelkhand region: Implications for crop management in the subtropics

Abstract Pollination stands out as the paramount ecosystem service facilitated by insect pollinators, providing benefits to over three quarters of the world’s major crops. The conservation and effective management of honeybees constitute integral components for ensuring the sustainable productivity of cross-pollinated crops. The present study aimed to document the potential pollinators of lucerne in the Bundelkhand region in India, which is known for its subtropical climatic conditions. Further, the foraging behaviour, including foraging rate and speed, was meticulously documented. A total of 13 insect species from seven families across three insect orders were observed visiting lucerne flowers. Hymenopterans comprised the majority of the floral visitors (73.48%), followed by Lepidoptera (26.51%) during the study period. Among them, Apis dorsata (Hymenoptera: Apidae) emerged as the most predominant floral visitor, exhibiting peak activity at 12:00 h with a density of 7.85 bees/m2/10 min. Surprisingly, among the major floral visitors, A. florea has the highest mean foraging rate (number of flowers visited/minute/forager), i.e., 11.56, 11.59 and 10.79 bees/flowers/minute during the initial flowering stage, peak flowering stage and late flowering stage, respectively. However, the foraging speed (time spent by the forager on each flower) was longest in A. dorsata, i.e., 5.05, 3.78 and 5.41 s/flower during initial, peak and late flowering stages, respectively. The findings emphasize the importance of enhancing honeybee activity to boost lucerne seed yield. Further, the outcomes of the study can be leveraged to optimize honeybee colony management, scheduling field operations such as pesticide application and weeding during periods of minimal honeybee activity.

Effect of Drought Stress on Agronomic Traits and Total Leaf Proteins in Different Bottle Gourd [<i>Lagenaria siceraria</i> (Molina) Standl.] Genotypes

Breeding drought-tolerant genotypes using genetic and biochemical tools is an important mitigation strategy to improve stress response and yields in bottle gourd [<em>Lagenaria siceraria</em> (Molina) Standl.]. This current study evaluated the variations among bottle gourd genotypes for potential breeding purposes by establishing the relationship between agronomic traits and the protein profile required for the plants’ resilience against drought stress. The study assessed 12 bottle gourd accessions grown under non-stressed (NS) control conditions and different levels of drought stress (DS) induced by withholding irrigation for 7, 14, and 21 days, using a 12 × 2 × 3 factorial experiment in a randomized complete block design with 3 replicates. Agronomic traits such as the total number of male and female flowers per plant, sex ratio, fruit number and fruit yield per plant (FYPP), and total protein analysis were determined in bottle-gourd plants immediately after the period of stress. Results showed significant differences (<em>p</em><em> </em>< 0.001) among the genotypes × environmental effect for most agronomic traits except the number of days to first flower (DTFF). Among the genotypes, BG-70 and BG-78 recorded the highest FYPP under drought stress conditions, with BG-70 showing similar results even under NS conditions. A positive correlation was found among all the agronomic traits and the total protein contents of the genotypes, especially after 14 days of drought stress. Overall, the results implied that the significant improvements in agronomic traits and unique protein expressions observed in BG-70 and BG-78 potentially confer tolerance to drought stress. Moreover, the high and unique proteins found in all genotypes (BG-48, BG-58, BG-52, BG-70, BG-78, and BG-81) warrant further research on their interaction with the stress, especially when coupled with improved agronomic traits, which could assist in identifying drought stress tolerant genotypes.